Part of Thermo Fisher Scientific

SERCA1 ATPase Monoclonal Antibody (VE121G9)

SERCA1 ATPase Antibody for IF, Western blot

Synonyms:
Sarcoplasmic or Endoplasmic Reticulum Calcium ATPase 1
Entrez Gene ID:
(Human) 487
UniProt ID:
O14983
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: VE121G9
Species Reactivity: Human (Hu) Murine (Ms) Rat (Rt) Canine (Ca) Rabbit (Rb) Amphibian (Am)
Immunogen: Purified rabbit skeletal muscle sarcoplasmic reticulum.
Ordering Information
Pierce SERCA1 ATPase Monoclonal Antibody (VE121G9)
Product Number Pkg. Size Price Purchase
MA3-912 100 µl $357.00
Add SERCA1 ATPase Monoclonal Antibody (VE121G9) to your cart.


Storage: -20º C, Avoid Freeze/Thaw Cycles
Form: 100 µl of PBS-diluted ascites containing 0.05% sodium azide.


Applications Dilution *
Western Blot (WB) 1:2,500
Immunofluorescence (IF) 1:500
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
MA3-912 detects sarcoplasmic or endoplasmic reticulum calcium 1 (SERCA1) ATPase in canine, amphibian, human, mouse, rat, and rabbit tissues.

MA3-912 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~110 kDa protein representing SERCA1 ATPase in mouse muscle extracts. Immunofluorescence staining of SERCA1 ATPase in canine skeletal muscle with MA3-912 results in strong labeling of the entire type II (fast) myofiber. MA3-912 has also been shown to inhibit the crystallization of SERCA ATPase induced by vanadate.

The MA3-912 antigen is purified rabbit skeletal muscle sarcoplasmic reticulum. This antibody recognizes an epitope between amino acid residues 506 and the C-terminus of rabbit skeletal muscle ATPase, a region that is exposed in native sarcoplasmic reticulum.
General Information
ATP dependent calcium pumps are responsible, in part, for the maintenance of low cytoplasmic free calcium concentrations. The ATP pumps that reside in intracellular organelles are encoded by a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulum calcium ATPases (SERCA). The SERCA1 gene is exclusively expressed in type II (fast) skeletal muscle. The SERCA2 gene is subject to tissue dependent processing which is responsible for the generation of SERCA2a muscle-specific form expressed in type I (slow) skeletal, cardiac and smooth muscle and the SERCA2b isoform expressed in all cell types. The SERCA3 gene is not as well characterized and is found in non-muscle cells.
PubMed References:
Blocking Assay
  Species / Dilution Summary
  Rb / 1:10
 MA3-912 was used in western blot, blocking/activating experiment, ELISA to investigate the functional properties of three anti-calcium ATPase antibodies
Biochim Biophys Acta. 1992 Jan 31;1103(2):281-95.
"Immunological relatedness of the sarcoplasmic reticulum Ca(2+)-ATPase and the Na+,K(+)-ATPase."
Author(s): Molnar E, Varga S, Jona I, Seidler NW, Martonosi A
Number of Citations: 1
(See PubMed article )
  Rb / Not Cited
 MA3-912 was used in blocking/activating experiment, ELISA and western blot to investigate the sarcoplasmic reticulum calcium ATPase through monoclonal and polyclonal antibodies
Biochim Biophys Acta. 1990 Apr 13;1023(2):147-67.
"The binding of monoclonal and polyclonal antibodies to the Ca2(+)-ATPase of sarcoplasmic reticulum: effects on interactions between ATPase molecules."
Author(s): Molnar E, Seidler NW, Jona I, Martonosi AN
Number of Citations: 5
(See PubMed article )
ELISA
  Species / Dilution Summary
  Rb / 1:100
 MA3-912 was used in western blot, blocking/activating experiment, ELISA to investigate the functional properties of three anti-calcium ATPase antibodies
Biochim Biophys Acta. 1992 Jan 31;1103(2):281-95.
"Immunological relatedness of the sarcoplasmic reticulum Ca(2+)-ATPase and the Na+,K(+)-ATPase."
Author(s): Molnar E, Varga S, Jona I, Seidler NW, Martonosi A
Number of Citations: 1
(See PubMed article )
  Rb / 1:1,000
 MA3-912 was used in blocking/activating experiment, ELISA and western blot to investigate the sarcoplasmic reticulum calcium ATPase through monoclonal and polyclonal antibodies
Biochim Biophys Acta. 1990 Apr 13;1023(2):147-67.
"The binding of monoclonal and polyclonal antibodies to the Ca2(+)-ATPase of sarcoplasmic reticulum: effects on interactions between ATPase molecules."
Author(s): Molnar E, Seidler NW, Jona I, Martonosi AN
Number of Citations: 5
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Ms / Not Cited
 MA3-912 was used in immunocytochemistry, immunoprecipitation and western blot to investigate the regulation of TGF-beta induced calcium influx by IP3RIII and its influence on actin cytoskeleton
Am J Physiol Renal Physiol. 2002 May;282(5):F910-20.
"TGF-beta-induced Ca(2+) influx involves the type III IP(3) receptor and regulates actin cytoskeleton."
Author(s): McGowan TA, Madesh M, Zhu Y, Wang L, Russo M, Deelman L, Henning R, Joseph S, Hajnoczky G, Sharma K
Number of Citations: 1
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Rt / Not Cited
 MA3-912 was used in immunohistochemistry to investigate the TRPC signalplex in rat ventricular myocytes
Am J Physiol Heart Circ Physiol. 2007 Feb;292(2):H874-83.
"TRPC3 channels colocalize with Na+/Ca2+ exchanger and Na+ pump in axial component of transverse-axial tubular system of rat ventricle."
Author(s): Goel M, Zuo CD, Sinkins WG, Schilling WP
Number of Citations: 1
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Ms / 1:500
 MA3-912 was used in immunocytochemistry, immunoprecipitation and western blot to investigate the regulation of TGF-beta induced calcium influx by IP3RIII and its influence on actin cytoskeleton
Am J Physiol Renal Physiol. 2002 May;282(5):F910-20.
"TGF-beta-induced Ca(2+) influx involves the type III IP(3) receptor and regulates actin cytoskeleton."
Author(s): McGowan TA, Madesh M, Zhu Y, Wang L, Russo M, Deelman L, Henning R, Joseph S, Hajnoczky G, Sharma K
Number of Citations: 1
(See PubMed article )
  Rb / Not Cited
 MA3-912 was used in western blot and immunoprecipitation to study the role of the NH2-terminal region of SERCA1a in SERCA1a protein folding and stabilization.
J Biol Chem. 1999 Aug 20;274(34):23910-5.
"Deletions or specific substitutions of a few residues in the NH(2)-terminal region (Ala(3) to Thr(9)) of sarcoplasmic reticulum Ca(2+)-ATPase cause inactivation and rapid degradation of the enzyme expressed in COS-1 cells."
Author(s): Daiho T, Yamasaki K, Suzuki H, Saino T, Kanazawa T
Number of Citations: 2
(See PubMed article )
Western Blot
  Species / Dilution Summary
  Am / Not Cited
 MA3-912 was used in western blot to investigate the low temperature molecular adaptation mechanism of the skeletal muscle SERCA 1 in the wood frog.
J Biol Chem. 2001 Feb 9;276(6):3911-9.
"Low temperature molecular adaptation of the skeletal muscle sarco(endo)plasmic reticulum Ca2+-ATPase 1 (SERCA 1) in the wood frog (Rana sylvatica)."
Author(s): Dode L, Van Baelen K, Wuytack F, Dean WL
Number of Citations: 1
(See PubMed article )
  Ca / 1:200
 MA3-912 was used in western blot, blocking/activating experiment, ELISA to investigate the functional properties of three anti-calcium ATPase antibodies
Biochim Biophys Acta. 1992 Jan 31;1103(2):281-95.
"Immunological relatedness of the sarcoplasmic reticulum Ca(2+)-ATPase and the Na+,K(+)-ATPase."
Author(s): Molnar E, Varga S, Jona I, Seidler NW, Martonosi A
Number of Citations: 1
(See PubMed article )
  Hu / 1:2,500
 MA3-912 was used in western blot to study how intermittent sprint training affects sarcoplasmic reticulum functions
Am J Physiol Regul Integr Comp Physiol. 2000 Jul;279(1):R152-60.
"Enhanced sarcoplasmic reticulum Ca(2+) release following intermittent sprint training."
Author(s): Ortenblad N, Lunde PK, Levin K, Andersen JL, Pedersen PK
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
 MA3-912 was used in western blot to investigate the importance of RyR2 complex formation in the development of a metabolic crisis in malignant hyperthermia
J Appl Physiol. 2004 Jan;96(1):11-8.
"Increased sensitivity of the ryanodine receptor to halothane-induced oligomerization in malignant hyperthermia-susceptible human skeletal muscle."
Author(s): Glover L, Heffron JJ, Ohlendieck K
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
 MA3-912 was used in western blot to study the correlation between muscle glycogen concentration with SR calcium regulation during prolonged moderate-intensity cycling exercise
Am J Physiol Regul Integr Comp Physiol. 2006 Oct;291(4):R1100-10.
"Manipulation of dietary carbohydrates after prolonged effort modifies muscle sarcoplasmic reticulum responses in exercising males."
Author(s): Duhamel TA, Perco JG, Green HJ
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
 MA3-912 was used in western blot to evaluate the effect of diet and exercise on muscle sarcoplasmic reticulum responses
Am J Physiol Cell Physiol. 2006 Oct;291(4):C607-17.
"Comparative effects of a low-carbohydrate diet and exercise plus a low-carbohydrate diet on muscle sarcoplasmic reticulum responses in males."
Author(s): Duhamel TA, Green HJ, Perco JG, Ouyang J
Number of Citations: 1
(See PubMed article )
  Hu / 1:2,500
 MA3-912 was used in western blot to study the impact of specific endurance training program on the expression of SERCA1 and SERCA2 proteins as well as on the myosin heavy chain composition in human vastus lateralis muscle
J Physiol Pharmacol. 2008 Sep;59(3):589-602.
"Training induced decrease in oxygen cost of cycling is accompanied by down-regulation of SERCA expression in human vastus lateralis muscle."
Author(s): Majerczak J, Karasinski J, Zoladz JA
Number of Citations: 0
(See PubMed article )
  Ms / Not Cited
 MA3-912 was used in western blot to investigate the mechanism for the defects in muscular dysgenesis
J Biol Chem. 1989 Jan 25;264(3):1345-8.
"Specific absence of the alpha 1 subunit of the dihydropyridine receptor in mice with muscular dysgenesis."
Author(s): Knudson CM, Chaudhari N, Sharp AH, Powell JA, Beam KG, Campbell KP
Number of Citations: 21
(See PubMed article )
  Ms / 1:2,500
 MA3-912 was used in western blot to investigate the role of thyroid hormone receptor (TR)-alpha1 and -beta on skeletal muscle function
Am J Physiol Regul Integr Comp Physiol. 2000 Mar;278(3):R598-603.
"Isometric force and endurance in soleus muscle of thyroid hormone receptor-alpha(1)- or -beta-deficient mice."
Author(s): Johansson C, Lännergren J, Lunde PK, Vennström B, Thorén P, Westerblad H
Number of Citations: 2
(See PubMed article )
  Ms / 1:2,000
 MA3-912 was used in immunocytochemistry, immunoprecipitation and western blot to investigate the regulation of TGF-beta induced calcium influx by IP3RIII and its influence on actin cytoskeleton
Am J Physiol Renal Physiol. 2002 May;282(5):F910-20.
"TGF-beta-induced Ca(2+) influx involves the type III IP(3) receptor and regulates actin cytoskeleton."
Author(s): McGowan TA, Madesh M, Zhu Y, Wang L, Russo M, Deelman L, Henning R, Joseph S, Hajnoczky G, Sharma K
Number of Citations: 1
(See PubMed article )
  Ms / 1:2,500
 MA3-912 was used in western blot to investigate the isometric force and endurance in the muscles of mice deficient in TRalpha1 or TRbeta.
J Physiol. 2003 Mar 15;547(Pt 3):789-96.
"Isometric force and endurance in skeletal muscle of mice devoid of all known thyroid hormone receptors."
Author(s): Johansson C, Lunde PK, Gothe S, Lannergren J, Westerblad H
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
 MA3-912 was used in western blot to examine the effects of development and cardiac hypertrophy on the sarcolipin expression in heart.
J Biol Chem. 2003 Mar 14;278(11):9570-5.
"Atrial chamber-specific expression of sarcolipin is regulated during development and hypertrophic remodeling."
Author(s): Minamisawa S, Wang Y, Chen J, Ishikawa Y, Chien KR, Matsuoka R
Number of Citations: 11
(See PubMed article )
  Ms / 1:5,000
 MA3-912 was used in western blot to study the role of the sodium/potassium-ATPase alpha2-subunit isoform during muscle contraction
Am J Physiol Cell Physiol. 2004 Nov;287(5):C1300-10.
"The Na(+)-K(+)-ATPase alpha2-subunit isoform modulates contractility in the perinatal mouse diaphragm."
Author(s): Radzyukevich TL, Moseley AE, Shelly DA, Redden GA, Behbehani MM, Lingrel JB, Paul RJ, Heiny JA
Number of Citations: 1
(See PubMed article )
  Rb / 1:200
 MA3-912 was used in western blot, blocking/activating experiment, ELISA to investigate the functional properties of three anti-calcium ATPase antibodies
Biochim Biophys Acta. 1992 Jan 31;1103(2):281-95.
"Immunological relatedness of the sarcoplasmic reticulum Ca(2+)-ATPase and the Na+,K(+)-ATPase."
Author(s): Molnar E, Varga S, Jona I, Seidler NW, Martonosi A
Number of Citations: 1
(See PubMed article )
  Rb / 1:1,000
 MA3-912 was used in blocking/activating experiment, ELISA and western blot to investigate the sarcoplasmic reticulum calcium ATPase through monoclonal and polyclonal antibodies
Biochim Biophys Acta. 1990 Apr 13;1023(2):147-67.
"The binding of monoclonal and polyclonal antibodies to the Ca2(+)-ATPase of sarcoplasmic reticulum: effects on interactions between ATPase molecules."
Author(s): Molnar E, Seidler NW, Jona I, Martonosi AN
Number of Citations: 5
(See PubMed article )
  Rb / Not Cited
 MA3-912 was used in western blot to investigate the mechanism for the defects in muscular dysgenesis
J Biol Chem. 1989 Jan 25;264(3):1345-8.
"Specific absence of the alpha 1 subunit of the dihydropyridine receptor in mice with muscular dysgenesis."
Author(s): Knudson CM, Chaudhari N, Sharp AH, Powell JA, Beam KG, Campbell KP
Number of Citations: 21
(See PubMed article )
  Rb / Not Cited
 MA3-912 was used in western blot and immunoprecipitation to study the role of the NH2-terminal region of SERCA1a in SERCA1a protein folding and stabilization.
J Biol Chem. 1999 Aug 20;274(34):23910-5.
"Deletions or specific substitutions of a few residues in the NH(2)-terminal region (Ala(3) to Thr(9)) of sarcoplasmic reticulum Ca(2+)-ATPase cause inactivation and rapid degradation of the enzyme expressed in COS-1 cells."
Author(s): Daiho T, Yamasaki K, Suzuki H, Saino T, Kanazawa T
Number of Citations: 2
(See PubMed article )
  Rb / Not Cited
 MA3-912 was used in western blot to study the exact disulfide structure of Cys876 and Cys888 of sarcoplasmic reticulum calcium-ATPase and to identify the possible role of the disulfide bond.
J Biol Chem. 2001 Aug 31;276(35):32771-8.
"Mutations of either or both Cys876 and Cys888 residues of sarcoplasmic reticulum Ca2+-ATPase result in a complete loss of Ca2+ transport activity without a loss of Ca2+-dependent ATPase activity. Role of the CYS876-CYS888 disulfide bond."
Author(s): Daiho T, Yamasaki K, Saino T, Kamidochi M, Satoh K, Iizuka H, Suzuki H
Number of Citations: 1
(See PubMed article )
  Rt / 1:2,500
 MA3-912 was used in western blot to study the contraction and intracellular calcium handling in rat skeletal muscle
Circ Res. 2001 Jun 22;88(12):1299-305.
"Contraction and intracellular Ca(2+) handling in isolated skeletal muscle of rats with congestive heart failure."
Author(s): Lunde PK, Dahlstedt AJ, Bruton JD, Lännergren J, Thorén P, Sejersted OM, Westerblad H
Number of Citations: 11
(See PubMed article )
  Rt / 1:4,000
 MA3-912 was used in western blot to study the interaction between the anti-apoptotic protein Bcl-2 and the SERCA
Biochem J. 2004 Oct 15;383(Pt 2):361-70.
"Anti-apoptotic protein Bcl-2 interacts with and destabilizes the sarcoplasmic/endoplasmic reticulum Ca2+-ATPase (SERCA)."
Author(s): Dremina ES, Sharov VS, Kumar K, Zaidi A, Michaelis EK, Schöneich C
Number of Citations: 1
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)