SERCA1 ATPase Antibody (VE121G9)

SERCA1 ATPase Monoclonal Antibody for Western blot, IF, IHC, IHC (F)

>> See 1 other antibody for ATP2A1 (Sarcoplasmic or Endoplasmic Reticulum Calcium ATPase 1)
Synonyms:
Sarcoplasmic or Endoplasmic Reticulum Calcium ATPase 1
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: VE121G9
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt), Amphibian (Am), Canine (Ca), Rabbit (Rb)
Published Species Reactivity: Amphibian (Am), Canine (Ca), Human (Hu), Mouse (Ms), Porcine (Po), Rabbit (Rb), Rat (Rt)
Immunogen: Purified rabbit skeletal muscle sarcoplasmic reticulum.
Ordering Information
Pierce SERCA1 ATPase Antibody (VE121G9)
Product #
MA3-912
Size
100 µl
Price
$360.00
Purchase
Add SERCA1 ATPase Antibody (VE121G9) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:2,500
Immunofluorescence (IF) 1:500
Immunohistochemistry (IHC) 1:20
Immunohistochemistry (Frozen) (IHC (F)) Assay Dependent
Published Applications Dilution
Western Blot (WB) See publications below
Immunocytochemistry (ICC) See publications below
Immunohistochemistry (IHC) See publications below
Immunoprecipitation (IP) See publications below
ELISA (ELISA) See publications below
Blocking Assay (BLOCK) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Storage Buffer: ascites diluted in PBS
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
MA3-912 detects sarcoplasmic or endoplasmic reticulum calcium 1 (SERCA1) ATPase in canine, amphibian, human, mouse, rat, and rabbit tissues.

MA3-912 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~110 kDa protein representing SERCA1 ATPase in mouse muscle extracts. Immunofluorescence staining of SERCA1 ATPase in canine skeletal muscle with MA3-912 results in strong labeling of the entire type II (fast) myofiber. MA3-912 has also been shown to inhibit the crystallization of SERCA ATPase induced by vanadate.

The MA3-912 antigen is purified rabbit skeletal muscle sarcoplasmic reticulum. This antibody recognizes an epitope between amino acid residues 506 and the C-terminus of rabbit skeletal muscle ATPase, a region that is exposed in native sarcoplasmic reticulum.
General Information
ATP dependent calcium pumps are responsible, in part, for the maintenance of low cytoplasmic free calcium concentrations. The ATP pumps that reside in intracellular organelles are encoded by a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulum calcium ATPases (SERCA). The SERCA1 gene is exclusively expressed in type II (fast) skeletal muscle. The SERCA2 gene is subject to tissue dependent processing which is responsible for the generation of SERCA2a muscle-specific form expressed in type I (slow) skeletal, cardiac and smooth muscle and the SERCA2b isoform expressed in all cell types. The SERCA3 gene is not as well characterized and is found in non-muscle cells.
Product Images
  • SERCA1 ATPase Antibody (MA3-912) in IF
    SERCA1-ATPase_MA3-912_Immunofluorescence_A2058-Cells.jpg

    Immunofluorescence with anti-SERCA1 ATPase Monoclonal Antibody [VE121G9] (MA3-912) (SERCA1-ATPase_MA3-912_Immunofluorescence_A2058-Cells.jpg)

    Immunofluorescence with anti-SERCA1 ATPase Monoclonal Antibody [VE121G9] (MA3-912)

    Immunofluorescent analysis of SERCA1 ATPase using Anti-SERCA1 ATPase Monoclonal Antibody (VE121G9) (Product# MA3-912) shows staining in A2058 Cells. SERCA1 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing SERCA1 ATPase (Product# MA3-912) at a dilution of 1:20 over night at 4 ◦C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35503, Goat Anti-Mouse). Images were taken at 60X magnification.
  • SERCA1 ATPase Antibody (MA3-912) in IF
    SERCA1-ATPase_MA3-912_Immunofluorescence_Hela-Cells.jpg

    Immunofluorescence with anti-SERCA1 ATPase Monoclonal Antibody [VE121G9] (MA3-912) (SERCA1-ATPase_MA3-912_Immunofluorescence_Hela-Cells.jpg)

    Immunofluorescence with anti-SERCA1 ATPase Monoclonal Antibody [VE121G9] (MA3-912)

    Immunofluorescent analysis of SERCA1 ATPase using Anti-SERCA1 ATPase Monoclonal Antibody (VE121G9) (Product# MA3-912) shows staining in Hela Cells. SERCA1 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing SERCA1 ATPase (Product# MA3-912) at a dilution of 1:20 over night at 4 ◦C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35503, Goat Anti-Mouse). Images were taken at 60X magnification.
  • SERCA1 ATPase Antibody (MA3-912) in IF
    SERCA1-ATPase_MA3-912_Immunofluorescence_U251-Cells.jpg

    Immunofluorescence with anti-SERCA1 ATPase Monoclonal Antibody [VE121G9] (MA3-912) (SERCA1-ATPase_MA3-912_Immunofluorescence_U251-Cells.jpg)

    Immunofluorescence with anti-SERCA1 ATPase Monoclonal Antibody [VE121G9] (MA3-912)

    Immunofluorescent analysis of SERCA1 ATPase using Anti-SERCA1 ATPase Monoclonal Antibody (VE121G9) (Product# MA3-912) shows staining in U251 Cells. SERCA1 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing SERCA1 ATPase (Product# MA3-912) at a dilution of 1:20 over night at 4 ◦C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35503, Goat Anti-Mouse). Images were taken at 60X magnification.
  • SERCA1 ATPase Antibody (MA3-912) in IHC
    MA3-912_Immunohistochemistry_Heart tissue.jpg

    Immunohistochemistry with anti-SERCA1 ATPase Monoclonal Antibody [VE121G9] (MA3-912) (MA3-912_Immunohistochemistry_Heart tissue.jpg)

    Immunohistochemistry with anti-SERCA1 ATPase Monoclonal Antibody [VE121G9] (MA3-912)

    Immunohistochemistry was performed on normal deparaffinized Human heart tissue tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing SERCA1 ATPase (MA3-912) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  • SERCA1 ATPase Antibody (MA3-912) in IHC
    MA3-912_Immunohistochemistry_Skeletal muscle.jpg

    Immunohistochemistry with anti-SERCA1 ATPase Monoclonal Antibody [VE121G9] (MA3-912) (MA3-912_Immunohistochemistry_Skeletal muscle.jpg)

    Immunohistochemistry with anti-SERCA1 ATPase Monoclonal Antibody [VE121G9] (MA3-912)

    Immunohistochemistry was performed on normal deparaffinized Human skeletal muscle tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing SERCA1 ATPase (MA3-912) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Publications:
Western Blot
  Species / Dilution Summary
  Am / Not Cited
MA3-912 was used in western blot to investigate the low temperature molecular adaptation mechanism of the skeletal muscle SERCA 1 in the wood frog.

J Biol Chem. 2001 Feb 9;276(6):3911-9.
"Low temperature molecular adaptation of the skeletal muscle sarco(endo)plasmic reticulum Ca2+-ATPase 1 (SERCA 1) in the wood frog (Rana sylvatica)."
Author(s): Dode L, Van Baelen K, Wuytack F, Dean WL
Number of Citations: 1
(See PubMed article )
  Ca / 1:200
MA3-912 was used in western blot, blocking/activating experiment, ELISA to investigate the functional properties of three anti-calcium ATPase antibodies

Biochim Biophys Acta. 1992 Jan 31;1103(2):281-95.
"Immunological relatedness of the sarcoplasmic reticulum Ca(2+)-ATPase and the Na+,K(+)-ATPase."
Author(s): Molnar E, Varga S, Jona I, Seidler NW, Martonosi A
Number of Citations: 1
(See PubMed article )
  Hu / 1:2,500
MA3-912 was used in western blot to study how intermittent sprint training affects sarcoplasmic reticulum functions

Am J Physiol Regul Integr Comp Physiol. 2000 Jul;279(1):R152-60.
"Enhanced sarcoplasmic reticulum Ca(2+) release following intermittent sprint training."
Author(s): Ortenblad N, Lunde PK, Levin K, Andersen JL, Pedersen PK
Number of Citations: 1
(See PubMed article )
  Hu / 0
MA3-912 was used in western blot to investigate the influence of exercise on SERCA function in human muscle

J Appl Physiol. 2003 Jul;95(1):138-44.
"Paradoxical effects of prior activity on human sarcoplasmic reticulum Ca2+-ATPase response to exercise."
Author(s): Tupling AR, Green HJ, Roy BD, Grant S, Ouyang J
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
MA3-912 was used in western blot to investigate the importance of RyR2 complex formation in the development of a metabolic crisis in malignant hyperthermia

J Appl Physiol. 2004 Jan;96(1):11-8.
"Increased sensitivity of the ryanodine receptor to halothane-induced oligomerization in malignant hyperthermia-susceptible human skeletal muscle."
Author(s): Glover L, Heffron JJ, Ohlendieck K
Number of Citations: 1
(See PubMed article )
  Hu / 1:3000
MA3-912 was used in western blot to investigate the metabolic and sarcoplasmic reticulum Ca2+ cycling responses in human muscle following prolonged exercise

Can J Physiol Pharmacol. 2005 Jul;83(7):643-55.
"Metabolic and sarcoplasmic reticulum Ca2+ cycling responses in human muscle 4 days following prolonged exercise."
Author(s): Duhamel TA, Green HJ, Perco JG, Ouyang J
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
MA3-912 was used in western blot to study the correlation between muscle glycogen concentration with SR calcium regulation during prolonged moderate-intensity cycling exercise

Am J Physiol Regul Integr Comp Physiol. 2006 Oct;291(4):R1100-10.
"Manipulation of dietary carbohydrates after prolonged effort modifies muscle sarcoplasmic reticulum responses in exercising males."
Author(s): Duhamel TA, Perco JG, Green HJ
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
MA3-912 was used in western blot to evaluate the effect of diet and exercise on muscle sarcoplasmic reticulum responses

Am J Physiol Cell Physiol. 2006 Oct;291(4):C607-17.
"Comparative effects of a low-carbohydrate diet and exercise plus a low-carbohydrate diet on muscle sarcoplasmic reticulum responses in males."
Author(s): Duhamel TA, Green HJ, Perco JG, Ouyang J
Number of Citations: 1
(See PubMed article )
  Hu / 1:2,500
MA3-912 was used in western blot to study the impact of specific endurance training program on the expression of SERCA1 and SERCA2 proteins as well as on the myosin heavy chain composition in human vastus lateralis muscle

J Physiol Pharmacol. 2008 Sep;59(3):589-602.
"Training induced decrease in oxygen cost of cycling is accompanied by down-regulation of SERCA expression in human vastus lateralis muscle."
Author(s): Majerczak J, Karasinski J, Zoladz JA
Number of Citations: 0
(See PubMed article )
  Hu / 0
MA3-912 was used in western blot to study the exercise-related skeletal muscle calcium homeostasis in normal and diseased hearts

Med Sci Sports Exerc. 2010 May;42(5):847-55.
"Training effects on skeletal muscle calcium handling in human chronic heart failure."
Author(s): Munkvik M, Rehn TA, Slettaløkken G, Hasic A, Hallén J, Sjaastad I, Sejersted OM, Lunde PK
Number of Citations: 1
(See PubMed article )
  Hu / 1:2500
MA3-912 was used in western blot to investigate the effect of endurance training on muscle metabolic stability

Exp Physiol. 2012 Mar;97(3):386-99.
"Endurance training decreases the non-linearity in the oxygen uptake-power output relationship in humans."
Author(s): Majerczak J, Korostynski M, Nieckarz Z, Szkutnik Z, Duda K, Zoladz JA
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
MA3-912 was used in western blot to investigate the mechanism for the defects in muscular dysgenesis

J Biol Chem. 1989 Jan 25;264(3):1345-8.
"Specific absence of the alpha 1 subunit of the dihydropyridine receptor in mice with muscular dysgenesis."
Author(s): Knudson CM, Chaudhari N, Sharp AH, Powell JA, Beam KG, Campbell KP
Number of Citations: 21
(See PubMed article )
  Ms / 1:2,500
MA3-912 was used in western blot to investigate the role of thyroid hormone receptor (TR)-alpha1 and -beta on skeletal muscle function

Am J Physiol Regul Integr Comp Physiol. 2000 Mar;278(3):R598-603.
"Isometric force and endurance in soleus muscle of thyroid hormone receptor-alpha(1)- or -beta-deficient mice."
Author(s): Johansson C, Lännergren J, Lunde PK, Vennström B, Thorén P, Westerblad H
Number of Citations: 2
(See PubMed article )
  Ms / 1:2,000
MA3-912 was used in immunocytochemistry, immunoprecipitation and western blot to investigate the regulation of TGF-beta induced calcium influx by IP3RIII and its influence on actin cytoskeleton

Am J Physiol Renal Physiol. 2002 May;282(5):F910-20.
"TGF-beta-induced Ca(2+) influx involves the type III IP(3) receptor and regulates actin cytoskeleton."
Author(s): McGowan TA, Madesh M, Zhu Y, Wang L, Russo M, Deelman L, Henning R, Joseph S, Hajnoczky G, Sharma K
Number of Citations: 1
(See PubMed article )
  Ms / 1:2,500
MA3-912 was used in western blot to investigate the isometric force and endurance in the muscles of mice deficient in TRalpha1 or TRbeta.

J Physiol. 2003 Mar 15;547(Pt 3):789-96.
"Isometric force and endurance in skeletal muscle of mice devoid of all known thyroid hormone receptors."
Author(s): Johansson C, Lunde PK, Gothe S, Lannergren J, Westerblad H
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
MA3-912 was used in western blot to examine the effects of development and cardiac hypertrophy on the sarcolipin expression in heart.

J Biol Chem. 2003 Mar 14;278(11):9570-5.
"Atrial chamber-specific expression of sarcolipin is regulated during development and hypertrophic remodeling."
Author(s): Minamisawa S, Wang Y, Chen J, Ishikawa Y, Chien KR, Matsuoka R
Number of Citations: 11
(See PubMed article )
  Ms / 1:5,000
MA3-912 was used in western blot to study the role of the sodium/potassium-ATPase alpha2-subunit isoform during muscle contraction

Am J Physiol Cell Physiol. 2004 Nov;287(5):C1300-10.
"The Na(+)-K(+)-ATPase alpha2-subunit isoform modulates contractility in the perinatal mouse diaphragm."
Author(s): Radzyukevich TL, Moseley AE, Shelly DA, Redden GA, Behbehani MM, Lingrel JB, Paul RJ, Heiny JA
Number of Citations: 1
(See PubMed article )
  Ms / 0
MA3-912 was used in western blot to investigate the role of M-band region of titin in muscle contraction

J Mol Biol. 2009 Oct 16;393(1):10-26.
"Altered contractility of skeletal muscle in mice deficient in titin's M-band region."
Author(s): Ottenheijm CA, Hidalgo C, Rost K, Gotthardt M, Granzier H
Number of Citations: 1
(See PubMed article )
  Ms / 0
MA3-912 was used in western blot to conduct proteomic profiling of extraocular muscles in Duchenne muscular dystrophy mouse model

Biochem Biophys Res Commun. 2010 Jun 11;396(4):1024-9.
"Proteomic profiling of naturally protected extraocular muscles from the dystrophin-deficient mdx mouse."
Author(s): Lewis C, Ohlendieck K
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
MA3-912 was used in western blot to identify myotonia-specific changes in protein expression in several mouse models

Mol Biosyst. 2011 Aug;7(8):2480-9.
"Identification of secondary effects of hyperexcitability by proteomic profiling of myotonic mouse muscle."
Author(s): Staunton L, Jockusch H, Wiegand C, Albrecht T, Ohlendieck K
Number of Citations: 2
(See PubMed article )
  Ms / 0
MA3-912 was used in immunohistochemistry and western blot to investigate the role of SERCAs in muscle contractile function

J Physiol. 2011 Dec 15;589(Pt 24):6139-55.
"Slowed relaxation and preserved maximal force in soleus muscles of mice with targeted disruption of the Serca2 gene in skeletal muscle."
Author(s): Sjåland C, Lunde PK, Swift F, Munkvik M, Ericsson M, Lunde M, Boye S, Christensen G, Ellingsen Ø, Sejersted OM, Andersson KB
Number of Citations: 1
(See PubMed article )
  Po / 0
MA3-912 was used in western blot to investigate the skeletal muscle sarcoplasmic reticulum proteins through microarray analyses

Biochim Biophys Acta. 2006 Sep;1764(9):1429-35.
"Microarray profiling of skeletal muscle sarcoplasmic reticulum proteins."
Author(s): Schulz JS, Palmer N, Steckelberg J, Jones SJ, Zeece MG
Number of Citations: 1
(See PubMed article )
  Rb / 1:200
MA3-912 was used in western blot, blocking/activating experiment, ELISA to investigate the functional properties of three anti-calcium ATPase antibodies

Biochim Biophys Acta. 1992 Jan 31;1103(2):281-95.
"Immunological relatedness of the sarcoplasmic reticulum Ca(2+)-ATPase and the Na+,K(+)-ATPase."
Author(s): Molnar E, Varga S, Jona I, Seidler NW, Martonosi A
Number of Citations: 1
(See PubMed article )
  Rb / 1:1,000
MA3-912 was used in blocking/activating experiment, ELISA and western blot to investigate the sarcoplasmic reticulum calcium ATPase through monoclonal and polyclonal antibodies

Biochim Biophys Acta. 1990 Apr 13;1023(2):147-67.
"The binding of monoclonal and polyclonal antibodies to the Ca2(+)-ATPase of sarcoplasmic reticulum: effects on interactions between ATPase molecules."
Author(s): Molnar E, Seidler NW, Jona I, Martonosi AN
Number of Citations: 5
(See PubMed article )
  Rb / Not Cited
MA3-912 was used in western blot to investigate the mechanism for the defects in muscular dysgenesis

J Biol Chem. 1989 Jan 25;264(3):1345-8.
"Specific absence of the alpha 1 subunit of the dihydropyridine receptor in mice with muscular dysgenesis."
Author(s): Knudson CM, Chaudhari N, Sharp AH, Powell JA, Beam KG, Campbell KP
Number of Citations: 21
(See PubMed article )
  Rb / Not Cited
MA3-912 was used in western blot and immunoprecipitation to study the role of the NH2-terminal region of SERCA1a in SERCA1a protein folding and stabilization.

J Biol Chem. 1999 Aug 20;274(34):23910-5.
"Deletions or specific substitutions of a few residues in the NH(2)-terminal region (Ala(3) to Thr(9)) of sarcoplasmic reticulum Ca(2+)-ATPase cause inactivation and rapid degradation of the enzyme expressed in COS-1 cells."
Author(s): Daiho T, Yamasaki K, Suzuki H, Saino T, Kanazawa T
Number of Citations: 2
(See PubMed article )
  Rb / Not Cited
MA3-912 was used in western blot to study the exact disulfide structure of Cys876 and Cys888 of sarcoplasmic reticulum calcium-ATPase and to identify the possible role of the disulfide bond.

J Biol Chem. 2001 Aug 31;276(35):32771-8.
"Mutations of either or both Cys876 and Cys888 residues of sarcoplasmic reticulum Ca2+-ATPase result in a complete loss of Ca2+ transport activity without a loss of Ca2+-dependent ATPase activity. Role of the CYS876-CYS888 disulfide bond."
Author(s): Daiho T, Yamasaki K, Saino T, Kamidochi M, Satoh K, Iizuka H, Suzuki H
Number of Citations: 1
(See PubMed article )
  Rb / 0
MA3-912 was used in western blot to study the oligomerization of skeletal muscle sarcoplasmic reticulum calcium-ATPase

Biochemistry. 2008 Dec 23;47(51):13711-25.
"Intermolecular interactions in the mechanism of skeletal muscle sarcoplasmic reticulum Ca(2+)-ATPase (SERCA1): evidence for a triprotomer."
Author(s): Mahaney JE, Thomas DD, Farrance IK, Froehlich JP
Number of Citations: 1
(See PubMed article )
  Rt / 1:2,500
MA3-912 was used in western blot to study the contraction and intracellular calcium handling in rat skeletal muscle

Circ Res. 2001 Jun 22;88(12):1299-305.
"Contraction and intracellular Ca(2+) handling in isolated skeletal muscle of rats with congestive heart failure."
Author(s): Lunde PK, Dahlstedt AJ, Bruton JD, Lännergren J, Thorén P, Sejersted OM, Westerblad H
Number of Citations: 11
(See PubMed article )
  Rt / 1:4,000
MA3-912 was used in western blot to study the interaction between the anti-apoptotic protein Bcl-2 and the SERCA

Biochem J. 2004 Oct 15;383(Pt 2):361-70.
"Anti-apoptotic protein Bcl-2 interacts with and destabilizes the sarcoplasmic/endoplasmic reticulum Ca2+-ATPase (SERCA)."
Author(s): Dremina ES, Sharov VS, Kumar K, Zaidi A, Michaelis EK, Schöneich C
Number of Citations: 1
(See PubMed article )
  Rt / 0
MA3-912 was used in western blot to investigate the influence of Bcl-2 on SERCA displacement and activity

Biochemistry. 2006 Jan 10;45(1):175-84.
"Displacement of SERCA from SR lipid caveolae-related domains by Bcl-2: a possible mechanism for SERCA inactivation."
Author(s): Dremina ES, Sharov VS, Schöneich C
Number of Citations: 1
(See PubMed article )
  Rt / 0
MA3-912 was used in western blot to investigate the expression of sarcalumenin and related calcium-regulatory proteins in rodent skeletal muscle during aging

Exp Gerontol. 2008 Oct;43(10):958-61.
"Reduced expression of sarcalumenin and related Ca2+ -regulatory proteins in aged rat skeletal muscle."
Author(s): O'Connell K, Gannon J, Doran P, Ohlendieck K
Number of Citations: 1
(See PubMed article )
  Rt / 0
MA3-912 was used in immunohistochemistry and western blot to investigate mitochondrial proteomic change during skeletal muscle aging

Proteomics. 2009 Dec;9(24):5509-24.
"Proteomic DIGE analysis of the mitochondria-enriched fraction from aged rat skeletal muscle."
Author(s): O'Connell K, Ohlendieck K
Number of Citations: 1
(See PubMed article )
  Rt / 1:2500
MA3-912 was used in western blot to study the role of calcium-handling proteins in the mechanical performance of rat skeletal muscle

J Exp Biol. 2011 Nov 1;214(Pt 21):3542-8.
"Variation in expression of calcium-handling proteins is associated with inter-individual differences in mechanical performance of rat (Rattus norvegicus) skeletal muscle."
Author(s): James RS, Walter I, Seebacher F
Number of Citations: 1
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Ms / Not Cited
MA3-912 was used in immunocytochemistry, immunoprecipitation and western blot to investigate the regulation of TGF-beta induced calcium influx by IP3RIII and its influence on actin cytoskeleton

Am J Physiol Renal Physiol. 2002 May;282(5):F910-20.
"TGF-beta-induced Ca(2+) influx involves the type III IP(3) receptor and regulates actin cytoskeleton."
Author(s): McGowan TA, Madesh M, Zhu Y, Wang L, Russo M, Deelman L, Henning R, Joseph S, Hajnoczky G, Sharma K
Number of Citations: 1
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Ca / 1:1000
MA3-912 was used in immunohistochemistry to investigate the level of SERCA1 in dilated cardiomyopathy canine myocardium

J Vet Cardiol. 2010 Apr;12(1):17-23.
"Immunohistochemical evidence for expression of fast-twitch type sarco(endo)plasmic reticulum Ca2+ ATPase (SERCA1) in German shepherd dogs with dilated cardiomyopathy myocardium."
Author(s): Summerfield N, Peters ME, Hercock CA, Mobasheri A, Young IS
Number of Citations: 1
(See PubMed article )
  Ms / 1:500
MA3-912 was used in immunohistochemistry to study the dynamics of sarcoplasmic reticulum Ca2+ and cytosolic cAMP in mouse skeletal muscle

J Cell Biol. 2006 Apr 24;173(2):187-93.
"Direct in vivo monitoring of sarcoplasmic reticulum Ca2+ and cytosolic cAMP dynamics in mouse skeletal muscle."
Author(s): Rudolf R, Magalhães PJ, Pozzan T
Number of Citations: 28
(See PubMed article )
  Ms / 0
MA3-912 was used in immunohistochemistry and western blot to investigate the role of SERCAs in muscle contractile function

J Physiol. 2011 Dec 15;589(Pt 24):6139-55.
"Slowed relaxation and preserved maximal force in soleus muscles of mice with targeted disruption of the Serca2 gene in skeletal muscle."
Author(s): Sjåland C, Lunde PK, Swift F, Munkvik M, Ericsson M, Lunde M, Boye S, Christensen G, Ellingsen Ø, Sejersted OM, Andersson KB
Number of Citations: 1
(See PubMed article )
  Ms / 0
MA3-912 was used in immunohistochemistry to investigate the myofibrillar disorganization in the myopathy of camptocormia

Acta Neuropathol. 2012 Mar;123(3):419-32.
"Myofibrillar disorganization characterizes myopathy of camptocormia in Parkinson's disease."
Author(s): Wrede A, Margraf NG, Goebel HH, Deuschl G, Schulz-Schaeffer WJ
Number of Citations: 1
(See PubMed article )
  Rt / Not Cited
MA3-912 was used in immunohistochemistry to investigate the TRPC signalplex in rat ventricular myocytes

Am J Physiol Heart Circ Physiol. 2007 Feb;292(2):H874-83.
"TRPC3 channels colocalize with Na+/Ca2+ exchanger and Na+ pump in axial component of transverse-axial tubular system of rat ventricle."
Author(s): Goel M, Zuo CD, Sinkins WG, Schilling WP
Number of Citations: 1
(See PubMed article )
  Rt / 0
MA3-912 was used in immunohistochemistry and western blot to investigate mitochondrial proteomic change during skeletal muscle aging

Proteomics. 2009 Dec;9(24):5509-24.
"Proteomic DIGE analysis of the mitochondria-enriched fraction from aged rat skeletal muscle."
Author(s): O'Connell K, Ohlendieck K
Number of Citations: 1
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Ms / 1:500
MA3-912 was used in immunocytochemistry, immunoprecipitation and western blot to investigate the regulation of TGF-beta induced calcium influx by IP3RIII and its influence on actin cytoskeleton

Am J Physiol Renal Physiol. 2002 May;282(5):F910-20.
"TGF-beta-induced Ca(2+) influx involves the type III IP(3) receptor and regulates actin cytoskeleton."
Author(s): McGowan TA, Madesh M, Zhu Y, Wang L, Russo M, Deelman L, Henning R, Joseph S, Hajnoczky G, Sharma K
Number of Citations: 1
(See PubMed article )
  Rb / Not Cited
MA3-912 was used in western blot and immunoprecipitation to study the role of the NH2-terminal region of SERCA1a in SERCA1a protein folding and stabilization.

J Biol Chem. 1999 Aug 20;274(34):23910-5.
"Deletions or specific substitutions of a few residues in the NH(2)-terminal region (Ala(3) to Thr(9)) of sarcoplasmic reticulum Ca(2+)-ATPase cause inactivation and rapid degradation of the enzyme expressed in COS-1 cells."
Author(s): Daiho T, Yamasaki K, Suzuki H, Saino T, Kanazawa T
Number of Citations: 2
(See PubMed article )
ELISA
  Species / Dilution Summary
  Rb / 1:100
MA3-912 was used in western blot, blocking/activating experiment, ELISA to investigate the functional properties of three anti-calcium ATPase antibodies

Biochim Biophys Acta. 1992 Jan 31;1103(2):281-95.
"Immunological relatedness of the sarcoplasmic reticulum Ca(2+)-ATPase and the Na+,K(+)-ATPase."
Author(s): Molnar E, Varga S, Jona I, Seidler NW, Martonosi A
Number of Citations: 1
(See PubMed article )
  Rb / 1:1,000
MA3-912 was used in blocking/activating experiment, ELISA and western blot to investigate the sarcoplasmic reticulum calcium ATPase through monoclonal and polyclonal antibodies

Biochim Biophys Acta. 1990 Apr 13;1023(2):147-67.
"The binding of monoclonal and polyclonal antibodies to the Ca2(+)-ATPase of sarcoplasmic reticulum: effects on interactions between ATPase molecules."
Author(s): Molnar E, Seidler NW, Jona I, Martonosi AN
Number of Citations: 5
(See PubMed article )
Blocking Assay
  Species / Dilution Summary
  Rb / 1:10
MA3-912 was used in western blot, blocking/activating experiment, ELISA to investigate the functional properties of three anti-calcium ATPase antibodies

Biochim Biophys Acta. 1992 Jan 31;1103(2):281-95.
"Immunological relatedness of the sarcoplasmic reticulum Ca(2+)-ATPase and the Na+,K(+)-ATPase."
Author(s): Molnar E, Varga S, Jona I, Seidler NW, Martonosi A
Number of Citations: 1
(See PubMed article )
  Rb / Not Cited
MA3-912 was used in blocking/activating experiment, ELISA and western blot to investigate the sarcoplasmic reticulum calcium ATPase through monoclonal and polyclonal antibodies

Biochim Biophys Acta. 1990 Apr 13;1023(2):147-67.
"The binding of monoclonal and polyclonal antibodies to the Ca2(+)-ATPase of sarcoplasmic reticulum: effects on interactions between ATPase molecules."
Author(s): Molnar E, Seidler NW, Jona I, Martonosi AN
Number of Citations: 5
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific