* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Antigen affinity chromatography
0.05% sodium azide
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Specific Information
PA5-23079 reacts with SDHB in human and mouse samples. The target sequence has 92% identity to rat, cow and pig, 85% identity to Xenopus and 84% identity to Zebrafish.
PA5-23079 has been successfully used in Western Blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry and Immunohistochemistry-Paraffin applications.
The PA5-23079 immunogen is a genomic peptide made to an N-terminal region of the human SDHB protein (within residues 1-150).
Part of the succinate dehydrogenase complex, SDHB is a mitochondrial protein that interfaces with the catalysis of succinate dehydrogenase. In many gastrointestinal tumors, SDHB is irregularly expressed, and leads to mutational activation of KIT. This may be as a result of elevated oxidative stress caused by mitochondrial dysfunction. In Dutch populations, there is a higher prevalence of mutations in the SDHB gene, and a higher overall level of hereditary paraganglioma and pheochromocytoma syndromes than in other Europeans. Similar trends in mutations in this gene seem to be related to atherosclerosis in American populations. Overall, the SDHB protein effects multiple cellular metabolism processes and it is not surprisingly linked to metabolism-related cellular dysfunctions.
SDHB Antibody (PA5-23079) in WB PA5-23079_WesternBlot.jpg
Western Blot with anti-SDHB Polyclonal Antibody (PA5-23079)