SAP102 Antibody

SAP102 Polyclonal Antibody for Western blot, IHC (F)

>> See 1 other antibody for Dlg3 (Synapse-Associated Protein 102, Discs large homolog 3, Neuroendocrine-DLG, XLMR)
Synonyms:
Synapse-Associated Protein 102, Discs large homolog 3, Neuroendocrine-DLG, XLMR
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Rat (Rt)
Published Species Reactivity: Mouse (Ms), Rat (Rt)
Immunogen: Synthetic peptide corresponding to residues G(83) P V P P K P V P G K N T P K L N G(100) of rat SAP102.
Ordering Information
Pierce SAP102 Antibody
Product #
PA1-045
Size
100 µg
Price
$316.00
Purchase
Add SAP102 Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 3 µg/ml
Immunohistochemistry (Frozen) (IHC (F)) 10 µg/ml
Published Applications Dilution
Western Blot (WB) See publications below
Immunocytochemistry (ICC) See publications below
Immunohistochemistry (IHC) See publications below
Immunoprecipitation (IP) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Antigen affinity chromatography
Storage Buffer: PBS with 1mg/ml BSA
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA1-045 detects synapse-associated protein 102 (SAP102) from rat tissues.

PA1-045 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects an ~102 kDa protein representing SAP102 in rat brain homogenate. Immunohistochemical staining of SAP102 in rat hippocampus yields a pattern consistent with post-synaptic localization.

The PA1-045 immunogen is a synthetic peptide corresponding to residues G(83) P V P P K P V P G K N T P K L N G(100) of rat SAP102. PA1-045 immunizing peptide (Cat. # PEP-023) is available for use in neutralization and control experiments.

General Information
Synapse-Associated Protein 102 (SAP102) is one of a family of plasma membrane-associated proteins found in synaptic junctions. Like other members of the family, SAP102 has three ~90 amino acid repeats called PDZ domains followed by an SH3 domain and a yeast guanylate kinase homology (GuK) domain. It is hypothesized that PDZ-domain interactions play a role in receptor and channel clustering which contributes to neuronal plasticity.

SAP102 is believed to participate in the clustering of certain proteins, including NMDA receptors, shaker-type potassium channels at the synaptic membrane in CNS neurons. NMDA receptors and Shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif. Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits) may interact with SAP102 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with SAP102 in vitro through a pseudo-homotypic PDZ-PDZ interaction.
Product Images
  • SAP102 Antibody (PA1-045) in WB
    PA1-045_1_07232010.jpg

    Western Blot with anti-SAP102 Polyclonal Antibody (PA1-045) (PA1-045_1_07232010.jpg)

    Western Blot with anti-SAP102 Polyclonal Antibody (PA1-045)

    Western blot detection of SAP102 on extract from COS cells transfected with rat SAP102 using PA1-045.
  • SAP102 Antibody (PA1-045) in IHC
    pa1-045_2_07232010.jpg

    Immunohistochemistry with anti-SAP102 Polyclonal Antibody (PA1-045) (pa1-045_2_07232010.jpg)

    Immunohistochemistry with anti-SAP102 Polyclonal Antibody (PA1-045)

    Immunohistochemical staining of Ammon's Horn and the dentate gyrus of the rat hippocampus using PA1-045.
Publications:
Western Blot
  Species / Dilution Summary
  Ms / 1:1000
PA1-045 was used in western blot to study the role of Cln8 gene in hippocampal excitabilit and the glutamatergic transmission system

J Neurosci Res. 2012 Jun;90(6):1148-58.
"Increased [³H]D-aspartate release and changes in glutamate receptor expression in the hippocampus of the mnd mouse."
Author(s): Bigini P, Milanese M, Gardoni F, Longhi A, Bonifacino T, Barbera S, Fumagalli E, Di Luca M, Mennini T, Bonanno G
Number of Citations: 0
(See PubMed article )
  Rt / Not Cited
PA1-045 was used in immunohistochemistry and western blot to investigate the association of SAP102 with NMDAR complex in rats

Neuron. 1996 Aug;17(2):255-65.
"SAP102, a novel postsynaptic protein that interacts with NMDA receptor complexes in vivo."
Author(s): Müller BM, Kistner U, Kindler S, Chung WJ, Kuhlendahl S, Fenster SD, Lau LF, Veh RW, Huganir RL, Gundelfinger ED, Garner CC
Number of Citations: 35
(See PubMed article )
  Rt / Not Cited
PA1-045 was used in western blot to investigate the interaction between the alpha2beta1 guanylyl cyclase isoform and PSD-95 in rat brain.

J Biol Chem. 2001 Nov 30;276(48):44647-52.
"Guanylyl cyclase/PSD-95 interaction: targeting of the nitric oxide-sensitive alpha2beta1 guanylyl cyclase to synaptic membranes."
Author(s): Russwurm M, Wittau N, Koesling D
Number of Citations: 1
(See PubMed article )
  Rt / Not Cited
PA1-045 was used in immunoprecipitation and western blot to investigate the roles of SAP97 and CaMKII in the location of Kv4.2

J Biol Chem. 2007 Sep 28;282(39):28691-9.
"SAP97 directs the localization of Kv4.2 to spines in hippocampal neurons: regulation by CaMKII."
Author(s): Gardoni F, Mauceri D, Marcello E, Sala C, Di Luca M, Jeromin A
Number of Citations: 1
(See PubMed article )
  Rt / 1:80
PA1-045 was used in immunocytochemistry and western blot to investigate the effect of different NMDAR subtypes on distinct synapses in the retinal circuitry

J Neurosci. 2009 Apr 1;29(13):4274-86.
"Subunit- and pathway-specific localization of NMDA receptors and scaffolding proteins at ganglion cell synapses in rat retina."
Author(s): Zhang J, Diamond JS
Number of Citations: 1
(See PubMed article )
  Rt / 1:1000
PA1-045 was used in western blot to investigate the effect of dopamine depletion on synaptic function and plasticity

J Neurosci. 2010 Oct 20;30(42):14182-93.
"Distinct levels of dopamine denervation differentially alter striatal synaptic plasticity and NMDA receptor subunit composition."
Author(s): Paillé V, Picconi B, Bagetta V, Ghiglieri V, Sgobio C, Di Filippo M, Viscomi MT, Giampà C, Fusco FR, Gardoni F, Bernardi G, Greengard P, Di Luca M, Calabresi P
Number of Citations: 4
(See PubMed article )
  Rt / Not Cited
PA1-045 was used in western blot to investigate the role of NADPH oxidase in the oxidative damage of postsynaptic density after transient cerebral ischemia and reperfusion

Mol Cell Neurosci. 2011 Mar;46(3):681-8.
"NADPH oxidase-mediated oxidative damage to proteins in the postsynaptic density after transient cerebral ischemia and reperfusion."
Author(s): Murotomi K, Takagi N, Takeo S, Tanonaka K
Number of Citations: 2
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Ms / Not Cited
PA1-045 was used in immunocytochemistry to study the relationship between neuronal potassium channels and PSD-95.

J Cell Biol. 2002 Nov 25;159(4):663-72.
"Clustering of neuronal potassium channels is independent of their interaction with PSD-95."
Author(s): Rasband MN, Park EW, Zhen D, Arbuckle MI, Poliak S, Peles E, Grant SG, Trimmer JS
Number of Citations: 1
(See PubMed article )
  Rt / 1:150
PA1-045 was used in immunocytochemistry and western blot to investigate the effect of different NMDAR subtypes on distinct synapses in the retinal circuitry

J Neurosci. 2009 Apr 1;29(13):4274-86.
"Subunit- and pathway-specific localization of NMDA receptors and scaffolding proteins at ganglion cell synapses in rat retina."
Author(s): Zhang J, Diamond JS
Number of Citations: 1
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Rt / Not Cited
PA1-045 was used in immunohistochemistry and western blot to investigate the association of SAP102 with NMDAR complex in rats

Neuron. 1996 Aug;17(2):255-65.
"SAP102, a novel postsynaptic protein that interacts with NMDA receptor complexes in vivo."
Author(s): Müller BM, Kistner U, Kindler S, Chung WJ, Kuhlendahl S, Fenster SD, Lau LF, Veh RW, Huganir RL, Gundelfinger ED, Garner CC
Number of Citations: 35
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Rt / Not Cited
PA1-045 was used in immunoprecipitation and western blot to investigate the roles of SAP97 and CaMKII in the location of Kv4.2

J Biol Chem. 2007 Sep 28;282(39):28691-9.
"SAP97 directs the localization of Kv4.2 to spines in hippocampal neurons: regulation by CaMKII."
Author(s): Gardoni F, Mauceri D, Marcello E, Sala C, Di Luca M, Jeromin A
Number of Citations: 1
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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