Part of Thermo Fisher Scientific

Ryanodine Receptor Monoclonal Antibody (C3-33)

Ryanodine Receptor Antibody for FACS, ICC, IF, IHC (F), IP, Western blot

Synonyms:
RyR
Entrez Gene ID:
(Human) 6261, (Murine) 20190, (Rat) 686059
UniProt ID:
P21817
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: C3-33
Species Reactivity: Human (Hu) Murine (Ms) Rat (Rt) Canine (Ca) Rabbit (Rb) Chicken (Ck) Amphibian (Am) Fish (Fs) Guinea Pig (GP)
Immunogen: Canine cardiac ryanodine receptor.
Ordering Information
Pierce Ryanodine Receptor Monoclonal Antibody (C3-33)
Product Number Pkg. Size Price Purchase
MA3-916 100 µg $357.00
Add Ryanodine Receptor Monoclonal Antibody (C3-33) to your cart.


Storage: -20º C, Avoid Freeze/Thaw Cycles
Form: 100 µg of antibody (1 mg/ml) in PBS-diluted ascites with 0.05% sodium azide.


Applications Dilution *
Immunocytochemistry (ICC) 1:50
Flow Cytometry (FACS) Assay dependent
Immunoprecipitation (IP) Assay dependent
Immunohistochemistry (Frozen) (IHC (F)) 1 µg/ml
Western Blot (WB) 1 µg/ml
Immunofluorescence (IF) Assay dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
MA3-916 detects ryanodine receptor 2 (RyR-2) and weakly detects RyR-1 in amphibian, canine, chicken, fish, guinea pig and rat tissues.

MA3-916 has been successfully used in Western blot, immunohistochemical, immunofluorescence, immunocytochemistry and immunoprecipitation procedures. By Western blot, this antibody detects a 565 kDa protein representing the ryanodine receptor. In non-mammalian vertebrates, a doublet is seen at 565 kDa representing the alpha and beta isoforms of the receptor. Immunohistochemical staining of RyR in rat cardiac tissue with MA3-916 results in intense staining of the myofiber, which is consistent with sarcoplasmic reticulum localization.

The MA3-916 antigen is canine cardiac ryanodine receptor.

Figure 1 shows immunolocalization of RyR1 in rat brain hippocampus using MA3-916.
General Information
The ryanodine receptor (RyR) is the channel responsible for the release of calcium from the sarcoplasmic reticulum (SR) in muscle cells and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains; the remainder of the protein, termed the "foot" region is located in the cytoplasm between the T-tubule and the SR. The mammalian RyR is the product of three different genes: RyR-1, which is expressed predominantly in skeletal muscle and areas of the brain, RyR-2, which is expressed predominantly in the heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain, and RyR-3 which is found in smooth muscle and the brain (striatum, thalamus and hippocampus).
Product Images
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  • IF using MA3-916
    Immunofluorescence with anti-Ryanodine Receptor Monoclonal Antibody [C3-33] (MA3-916)

    Immunofluorescence with anti-Ryanodine Receptor Monoclonal Antibody [C3-33] (MA3-916)

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PubMed References:
ELISA
  Species / Dilution Summary
  Rb / Not Cited
 MA3-916 was used in ELISA and western blot to investigate the accessibility of RyR1 domains to specific antibodies
J Biol Chem. 1995 May 12;270(19):11338-47.
"Lumenal sites and C terminus accessibility of the skeletal muscle calcium release channel (ryanodine receptor)."
Author(s): Grunwald R, Meissner G
Number of Citations: 14
(See PubMed article )
  Rb / Not Cited
 MA3-916 was used in ELISA and western blot to study the role of a highly conserved sequence of the calcium release channel in ryanodine and caffeine binding and activation.
J Biol Chem. 2001 Aug 24;276(34):31760-71.
"Functional characterization of mutants in the predicted pore region of the rabbit cardiac muscle Ca(2+) release channel (ryanodine receptor isoform 2)."
Author(s): Du GG, Guo X, Khanna VK, MacLennan DH
Number of Citations: 1
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Ca / 1:500
 MA3-916 was used in immunocytochemistry to determine whether IP3Rs contribute to the generation of wide long lasting perinuclear calcium release events in canine Purkinje cells
J Mol Cell Cardiol. 2008 Aug;45(2):176-84.
"Wide long lasting perinuclear Ca2+ release events generated by an interaction between ryanodine and IP3 receptors in canine Purkinje cells."
Author(s): Hirose M, Stuyvers B, Dun W, Ter Keurs H, Boyden PA
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
 MA3-916 was used in immunocytochemistry to demonstrate the different properties of RyR2 and RyR1 are retained in the recombinant expressed proteins
J Biol Chem. 1998 Dec 11;273(50):33259-66.
"Characterization of recombinant rabbit cardiac and skeletal muscle Ca2+ release channels (ryanodine receptors) with a novel [3H]ryanodine binding assay."
Author(s): Du GG, Imredy JP, MacLennan DH
Number of Citations: 15
(See PubMed article )
  Hu / Not Cited
 MA3-916 was used in immunocytochemistry to investigate the role of RyR2 in beta-cell survival in vitro and its mechanism .
J Biol Chem. 2004 Jun 4;279(23):24794-802.
"RyR2 and calpain-10 delineate a novel apoptosis pathway in pancreatic islets."
Author(s): Johnson JD, Han Z, Otani K, Ye H, Zhang Y, Wu H, Horikawa Y, Misler S, Bell GI, Polonsky KS
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
 MA3-916 was used in immunocytochemistry to investigate the role of RyR during cardiomyocyte development.
Proc Natl Acad Sci U S A. 2002 Jul 9;99(14):9225-30.
"The ryanodine receptor modulates the spontaneous beating rate of cardiomyocytes during development."
Author(s): Yang HT, Tweedie D, Wang S, Guia A, Vinogradova T, Bogdanov K, Allen PD, Stern MD, Lakatta EG, Boheler KR
Number of Citations: 1
(See PubMed article )
  Ms / 1:50
 MA3-916 was used in western blot and immunocytochemistry to investigate the differential distribution of inositol (1,4,5)-trisphosphate receptors and calcium signaling in rod bipolar cells.
Invest Ophthalmol Vis Sci. 2005 Jan;46(1):292-8.
"Differentially distributed IP3 receptors and Ca2+ signaling in rod bipolar cells."
Author(s): Koulen P, Wei J, Madry C, Liu J, Nixon E
Number of Citations: 1
(See PubMed article )
  Ms / 10 ng/ul
 MA3-916 was used in western blot and immunocytochemistry to study the expression of ryanodine receptors in mouse airway smooth muscle cells.
J Biol Chem. 2005 Jul 15;280(28):26287-94.
"Ryanodine receptors in muscarinic receptor-mediated bronchoconstriction."
Author(s): Du W, Stiber JA, Rosenberg PB, Meissner G, Eu JP
Number of Citations: 1
(See PubMed article )
  Ms / 1:200
 MA3-916 was used in immunocytochemistry and western blot to identify the function of intracellular calciumchannels on the regulation of specific cellular functions in mouse lacrimal gland acinar cells
Open Ophthalmol J. 2007 Dec 4;1():8-16.
"Identification and functional distribution of intracellular ca channels in mouse lacrimal gland acinar cells."
Author(s): Medina-Ortiz WE, Gregg EV, Brun-Zinkernagel AM, Koulen P
Number of Citations: 1
(See PubMed article )
  Po / Not Cited
 MA3-916 was used in immunocytochemistry to study the distribution of SR calcium release in pig ventricular myocytes
Circ Res. 2002 Nov 29;91(11):1023-30.
"Spatial and temporal inhomogeneities during Ca2+ release from the sarcoplasmic reticulum in pig ventricular myocytes."
Author(s): Heinzel FR, Bito V, Volders PG, Antoons G, Mubagwa K, Sipido KR
Number of Citations: 4
(See PubMed article )
  Rt / Not Cited
 MA3-916 was used in immunocytochemistry to investigate the subcellular localization of A-kinase anchoring protein 100 (AKAP100) in the adult rat heart
J Cell Biol. 1998 Jul 27;142(2):511-22.
"A-kinase anchoring protein 100 (AKAP100) is localized in multiple subcellular compartments in the adult rat heart."
Author(s): Yang J, Drazba JA, Ferguson DG, Bond M
Number of Citations: 16
(See PubMed article )
  Rt / 1:100
 MA3-916 was used in immunocytochemistry to investigate the local sarcoplasmic reticulum calcium signalling initiation in cerebral arteries
Circ Res. 1998 Nov 30;83(11):1104-14.
"Ontogeny of local sarcoplasmic reticulum Ca2+ signals in cerebral arteries: Ca2+ sparks as elementary physiological events."
Author(s): Gollasch M, Wellman GC, Knot HJ, Jaggar JH, Damon DH, Bonev AD, Nelson MT
Number of Citations: 15
(See PubMed article )
  Rt / 1:150
 MA3-916 was used in immunocytochemistry to investigate the change of intracellular calcium during the process of RASMC proliferation
Arterioscler Thromb Vasc Biol. 2000 May;20(5):1225-35.
"Intracellular Ca(2+) handling in vascular smooth muscle cells is affected by proliferation."
Author(s): Vallot O, Combettes L, Jourdon P, Inamo J, Marty I, Claret M, Lompré AM
Number of Citations: 11
(See PubMed article )
  Rt / Not Cited
 MA3-916 was used in immunocytochemistry, immunoprecipitation and western blot to study the role of mAKAP and ryanodine receptor in cardiomyocyte nuclear envelope signalling.
J Cell Sci. 2001 Sep;114(Pt 17):3167-76.
"mAKAP and the ryanodine receptor are part of a multi-component signaling complex on the cardiomyocyte nuclear envelope."
Author(s): Kapiloff MS, Jackson N, Airhart N
Number of Citations: 14
(See PubMed article )
  Rt / 1:500
 MA3-916 was used in immunocytochemistry to investigate the role of distinct subcellular location of the calcium-binding protein S100A1 in cardiac calcium-handling
J Cell Sci. 2005 Jan 15;118(Pt 2):421-31.
"Distinct subcellular location of the Ca2+-binding protein S100A1 differentially modulates Ca2+-cycling in ventricular rat cardiomyocytes."
Author(s): Most P, Boerries M, Eicher C, Schweda C, Völkers M, Wedel T, Söllner S, Katus HA, Remppis A, Aebi U, Koch WJ, Schoenenberger CA
Number of Citations: 1
(See PubMed article )
  Rt / Not Cited
 MA3-916 was used in immunocytochemistry to investigate the localization of caveolin-3
Biophys J. 2005 Sep;89(3):1893-901.
"Caveolin-3 is adjacent to a group of extradyadic ryanodine receptors."
Author(s): Scriven DR, Klimek A, Asghari P, Bellve K, Moore ED
Number of Citations: 1
(See PubMed article )
  Rt / 1:100
 MA3-916 was used in immunocytochemistry and immunohistochemistry to investigate the localizeion of RyR2 clusters in rat ventricular and atrial myocytes in three dimensions
Biophys J. 2006 Jul 1;91(1):1-13.
"Three-dimensional distribution of ryanodine receptor clusters in cardiac myocytes."
Author(s): Chen-Izu Y, McCulle SL, Ward CW, Soeller C, Allen BM, Rabang C, Cannell MB, Balke CW, Izu LT
Number of Citations: 1
(See PubMed article )
  Rt / 1:100
 MA3-916 was used in western blot and immunocytochemistry to study excitation-contraction coupling mechanisms in striated and smooth muscles.
J Biol Chem. 2006 Oct 6;281(40):30143-51.
"Excitation-contraction coupling in airway smooth muscle."
Author(s): Du W, McMahon TJ, Zhang ZS, Stiber JA, Meissner G, Eu JP
Number of Citations: 1
(See PubMed article )
  Rt / Not Cited
 MA3-916 was used in immunocytochemistry to study the functions of ryanodine receptor 2 in rat ventricular cells.
J Physiol. 2007 Aug 15;583(Pt 1):251-69.
"Functional groups of ryanodine receptors in rat ventricular cells."
Author(s): Lukyanenko V, Ziman A, Lukyanenko A, Salnikov V, Lederer WJ
Number of Citations: 1
(See PubMed article )
  Rt / Not Cited
 MA3-916 was used in immunocytochemistry and immunohistochemistry to study the relationship between intracellular calcium release via RyR2 with hypertension-induced cardiac hypertrophy and heart failure
Am J Physiol Heart Circ Physiol. 2007 Oct;293(4):H2409-17.
"Phosphorylation of RyR2 and shortening of RyR2 cluster spacing in spontaneously hypertensive rat with heart failure."
Author(s): Chen-Izu Y, Ward CW, Stark W Jr, Banyasz T, Sumandea MP, Balke CW, Izu LT, Wehrens XH
Number of Citations: 1
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Am / Not Cited
 MA3-916 was used in immunohistochemistry, western blot and immunoprecipitation to investigate and characterize RyR isoforms in different animal species
Am J Physiol. 1992 Aug;263(2 Pt 1):C365-72.
"Amphibian ryanodine receptor isoforms are related to those of mammalian skeletal or cardiac muscle."
Author(s): Lai FA, Liu QY, Xu L, el-Hashem A, Kramarcy NR, Sealock R, Meissner G
Number of Citations: 22
(See PubMed article )
  Ca / Not Cited
 MA3-916 was used in immunohistochemistry to characterize the cardioprotective drug K201 .
Circulation. 2006 Jan 17;113(2):246-51.
"New cardioprotective agent K201 is natriuretic and glomerular filtration rate enhancing."
Author(s): Lisy O, Burnett JC Jr
Number of Citations: 1
(See PubMed article )
  Ck / 0.1 ug/ml
 MA3-916 was used in immunohistochemistry and western blot to investigate the expression and distribution of ryanodine receptor in avian cardiac muscle
J Biol Chem. 1994 Jan 21;269(3):1627-34.
"Extended junctional sarcoplasmic reticulum of avian cardiac muscle contains functional ryanodine receptors."
Author(s): Junker J, Sommer JR, Sar M, Meissner G
Number of Citations: 13
(See PubMed article )
  Hu / Not Cited
 MA3-916 was used in immunohistochemistry and western blot to examine the expression of isoforms of SR calcium-release channel in the human heart and quantify the corresponding expression changes due to cardiac failure
Circulation. 2001 Jun 5;103(22):2739-44.
"Increased expression of isoform 1 of the sarcoplasmic reticulum Ca(2+)-release channel in failing human heart."
Author(s): Münch G, Bölck B, Sugaru A, Brixius K, Bloch W, Schwinger RH
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
 MA3-916 was used in immunohistochemistry to study the expression of ryanodine receptor clusters in rat and human cardiac myocytes
Proc Natl Acad Sci U S A. 2007 Sep 18;104(38):14958-63.
"Analysis of ryanodine receptor clusters in rat and human cardiac myocytes."
Author(s): Soeller C, Crossman D, Gilbert R, Cannell MB
Number of Citations: 1
(See PubMed article )
  Ms / 1:100
 MA3-916 was used in immunohistochemistry to suggest that ET-1 activation of calcium sparks is mediated via the ETA receptor-PLC-IP3 pathway
Am J Physiol Lung Cell Mol Physiol. 2003 Sep;285(3):L680-90.
"ET-1 activates Ca2+ sparks in PASMC: local Ca2+ signaling between inositol trisphosphate and ryanodine receptors."
Author(s): Zhang WM, Yip KP, Lin MJ, Shimoda LA, Li WH, Sham JS
Number of Citations: 1
(See PubMed article )
  Rb / Not Cited
 MA3-916 was used in immunohistochemistry and western blot to investigate the distribution of sarcolemmal DHPR, RyR and triadin in adult rabbit ventricle and atrium
J Cell Biol. 1995 May;129(3):673-82.
"Immunolocalization of sarcolemmal dihydropyridine receptor and sarcoplasmic reticular triadin and ryanodine receptor in rabbit ventricle and atrium."
Author(s): Carl SL, Felix K, Caswell AH, Brandt NR, Ball WJ Jr, Vaghy PL, Meissner G, Ferguson DG
Number of Citations: 47
(See PubMed article )
  Rb / 1:100-1:200
 MA3-916 was used in immunohistochemistry and western blot to study the localization of DHPR and RyR in rabbit myocarial cells during ontogeny
Am J Physiol Heart Circ Physiol. 2000 Jul;279(1):H202-9.
"Colocalization of dihydropyridine and ryanodine receptors in neonate rabbit heart using confocal microscopy."
Author(s): Sedarat F, Xu L, Moore ED, Tibbits GF
Number of Citations: 8
(See PubMed article )
  Rt / 1-5 ug/ml
 MA3-916 was used in immunohistochemistry to study the distribution and expression level of the cardiac RyR isoform in mammalian brain
Biochem J. 1992 Dec 1;288 ( Pt 2)():553-64.
"Expression of a cardiac Ca(2+)-release channel isoform in mammalian brain."
Author(s): Lai FA, Dent M, Wickenden C, Xu L, Kumari G, Misra M, Lee HB, Sar M, Meissner G
Number of Citations: 11
(See PubMed article )
  Rt / 1 ug/ml
 MA3-916 was used in immunohistochemistry and western blot to investigate the expression and distribution of ryanodine receptor in avian cardiac muscle
J Biol Chem. 1994 Jan 21;269(3):1627-34.
"Extended junctional sarcoplasmic reticulum of avian cardiac muscle contains functional ryanodine receptors."
Author(s): Junker J, Sommer JR, Sar M, Meissner G
Number of Citations: 13
(See PubMed article )
  Rt / 1:200
 MA3-916 was used in immunocytochemistry and immunohistochemistry to investigate the localizeion of RyR2 clusters in rat ventricular and atrial myocytes in three dimensions
Biophys J. 2006 Jul 1;91(1):1-13.
"Three-dimensional distribution of ryanodine receptor clusters in cardiac myocytes."
Author(s): Chen-Izu Y, McCulle SL, Ward CW, Soeller C, Allen BM, Rabang C, Cannell MB, Balke CW, Izu LT
Number of Citations: 1
(See PubMed article )
  Rt / Not Cited
 MA3-916 was used in immunohistochemistry and western blot to investigate the TRPC signalplex in rat ventricular myocytes
Am J Physiol Heart Circ Physiol. 2007 Feb;292(2):H874-83.
"TRPC3 channels colocalize with Na+/Ca2+ exchanger and Na+ pump in axial component of transverse-axial tubular system of rat ventricle."
Author(s): Goel M, Zuo CD, Sinkins WG, Schilling WP
Number of Citations: 1
(See PubMed article )
  Rt / 1:200
 MA3-916 was used in immunocytochemistry and immunohistochemistry to study the relationship between intracellular calcium release via RyR2 with hypertension-induced cardiac hypertrophy and heart failure
Am J Physiol Heart Circ Physiol. 2007 Oct;293(4):H2409-17.
"Phosphorylation of RyR2 and shortening of RyR2 cluster spacing in spontaneously hypertensive rat with heart failure."
Author(s): Chen-Izu Y, Ward CW, Stark W Jr, Banyasz T, Sumandea MP, Balke CW, Izu LT, Wehrens XH
Number of Citations: 1
(See PubMed article )
  Rt / Not Cited
 MA3-916 was used in immunohistochemistry to study the expression of ryanodine receptor clusters in rat and human cardiac myocytes
Proc Natl Acad Sci U S A. 2007 Sep 18;104(38):14958-63.
"Analysis of ryanodine receptor clusters in rat and human cardiac myocytes."
Author(s): Soeller C, Crossman D, Gilbert R, Cannell MB
Number of Citations: 1
(See PubMed article )
  Rt / Not Cited
 MA3-916 was used in immunohistochemistry to study the mechanism for the calcium transport between reticulum subdomains and the mitochondria
J Biol Chem. 2008 Nov 21;283(47):32771-80.
"Physical coupling supports the local Ca2+ transfer between sarcoplasmic reticulum subdomains and the mitochondria in heart muscle."
Author(s): García-Pérez C, Hajnóczky G, Csordás G
Number of Citations: 1
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Ca / 0.010-0.5 mg/ml
 MA3-916 was used in immunohistochemistry, western blot and immunoprecipitation to investigate and characterize RyR isoforms in different animal species
Am J Physiol. 1992 Aug;263(2 Pt 1):C365-72.
"Amphibian ryanodine receptor isoforms are related to those of mammalian skeletal or cardiac muscle."
Author(s): Lai FA, Liu QY, Xu L, el-Hashem A, Kramarcy NR, Sealock R, Meissner G
Number of Citations: 22
(See PubMed article )
  Ca / 1:1,000
 MA3-916 was used in western blot and immunoprecipitation to investigate the effect of polycystin-2 on ryanodine receptor-dependent calcium signaling.
Proc Natl Acad Sci U S A. 2007 Apr 10;104(15):6454-9.
"Regulation of ryanodine receptor-dependent calcium signaling by polycystin-2."
Author(s): Anyatonwu GI, Estrada M, Tian X, Somlo S, Ehrlich BE
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
 MA3-916 was used in immunocytochemistry, immunoprecipitation and western blot to study the role of mAKAP and ryanodine receptor in cardiomyocyte nuclear envelope signalling.
J Cell Sci. 2001 Sep;114(Pt 17):3167-76.
"mAKAP and the ryanodine receptor are part of a multi-component signaling complex on the cardiomyocyte nuclear envelope."
Author(s): Kapiloff MS, Jackson N, Airhart N
Number of Citations: 14
(See PubMed article )
  Ms / Not Cited
 MA3-916 was used in immunoprecipitation to investigate the role of inositol 1,4,5-trisphosphate during the regulation of Ca2.
J Biol Chem. 2002 Jan 11;277(2):1340-8.
"Phosphorylation of inositol 1,4,5-trisphosphate receptors in parotid acinar cells. A mechanism for the synergistic effects of cAMP on Ca2+ signaling."
Author(s): Bruce JI, Shuttleworth TJ, Giovannucci DR, Yule DI
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
 MA3-916 was used in immunoprecipitation and western blot to study the effect of the phosphorylation of ryanodine receptor
Circ Res. 2006 Aug 18;99(4):398-406.
"Ca2+/Calmodulin-dependent protein kinase II phosphorylation of ryanodine receptor does affect calcium sparks in mouse ventricular myocytes."
Author(s): Guo T, Zhang T, Mestril R, Bers DM
Number of Citations: 1
(See PubMed article )
  Ms / 1:1,000
 MA3-916 was used in western blot and immunoprecipitation to investigate the effect of polycystin-2 on ryanodine receptor-dependent calcium signaling.
Proc Natl Acad Sci U S A. 2007 Apr 10;104(15):6454-9.
"Regulation of ryanodine receptor-dependent calcium signaling by polycystin-2."
Author(s): Anyatonwu GI, Estrada M, Tian X, Somlo S, Ehrlich BE
Number of Citations: 1
(See PubMed article )
  Rb / 0.010-0.5 mg/ml
 MA3-916 was used in immunohistochemistry, western blot and immunoprecipitation to investigate and characterize RyR isoforms in different animal species
Am J Physiol. 1992 Aug;263(2 Pt 1):C365-72.
"Amphibian ryanodine receptor isoforms are related to those of mammalian skeletal or cardiac muscle."
Author(s): Lai FA, Liu QY, Xu L, el-Hashem A, Kramarcy NR, Sealock R, Meissner G
Number of Citations: 22
(See PubMed article )
  Rb / Not Cited
 MA3-916 was used in immunoprecipitation to investigate the relationship between endogenous CaMKII with RyR2
Biochem J. 2004 Jan 15;377(Pt 2):357-66.
"Calcium/calmodulin-dependent protein kinase IIdelta associates with the ryanodine receptor complex and regulates channel function in rabbit heart."
Author(s): Currie S, Loughrey CM, Craig MA, Smith GL
Number of Citations: 1
(See PubMed article )
Western Blot
  Species / Dilution Summary
  Am / 1:10
 MA3-916 was used in immunohistochemistry, western blot and immunoprecipitation to investigate and characterize RyR isoforms in different animal species
Am J Physiol. 1992 Aug;263(2 Pt 1):C365-72.
"Amphibian ryanodine receptor isoforms are related to those of mammalian skeletal or cardiac muscle."
Author(s): Lai FA, Liu QY, Xu L, el-Hashem A, Kramarcy NR, Sealock R, Meissner G
Number of Citations: 22
(See PubMed article )
  Am / Not Cited
 MA3-916 was used in western blot to purify RyR from stomach smooth muscle cells of the toad Bufo marinus and study its functional properties
Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):3294-8.
"Evidence for a Ca(2+)-gated ryanodine-sensitive Ca2+ release channel in visceral smooth muscle."
Author(s): Xu L, Lai FA, Cohn A, Etter E, Guerrero A, Fay FS, Meissner G
Number of Citations: 11
(See PubMed article )
  Ca / 1:10
 MA3-916 was used in immunohistochemistry, western blot and immunoprecipitation to investigate and characterize RyR isoforms in different animal species
Am J Physiol. 1992 Aug;263(2 Pt 1):C365-72.
"Amphibian ryanodine receptor isoforms are related to those of mammalian skeletal or cardiac muscle."
Author(s): Lai FA, Liu QY, Xu L, el-Hashem A, Kramarcy NR, Sealock R, Meissner G
Number of Citations: 22
(See PubMed article )
  Ca / Not Cited
 MA3-916 was used in western blot to purify RyR from stomach smooth muscle cells of the toad Bufo marinus and study its functional properties
Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):3294-8.
"Evidence for a Ca(2+)-gated ryanodine-sensitive Ca2+ release channel in visceral smooth muscle."
Author(s): Xu L, Lai FA, Cohn A, Etter E, Guerrero A, Fay FS, Meissner G
Number of Citations: 11
(See PubMed article )
  Ca / Not Cited
 MA3-916 was used in immunohistochemistry and western blot to investigate the expression and distribution of ryanodine receptor in avian cardiac muscle
J Biol Chem. 1994 Jan 21;269(3):1627-34.
"Extended junctional sarcoplasmic reticulum of avian cardiac muscle contains functional ryanodine receptors."
Author(s): Junker J, Sommer JR, Sar M, Meissner G
Number of Citations: 13
(See PubMed article )
  Ca / Not Cited
 MA3-916 was used in western blot and immunoprecipitation to investigate the effect of polycystin-2 on ryanodine receptor-dependent calcium signaling.
Proc Natl Acad Sci U S A. 2007 Apr 10;104(15):6454-9.
"Regulation of ryanodine receptor-dependent calcium signaling by polycystin-2."
Author(s): Anyatonwu GI, Estrada M, Tian X, Somlo S, Ehrlich BE
Number of Citations: 1
(See PubMed article )
  Ck / Not Cited
 MA3-916 was used in immunohistochemistry and western blot to investigate the expression and distribution of ryanodine receptor in avian cardiac muscle
J Biol Chem. 1994 Jan 21;269(3):1627-34.
"Extended junctional sarcoplasmic reticulum of avian cardiac muscle contains functional ryanodine receptors."
Author(s): Junker J, Sommer JR, Sar M, Meissner G
Number of Citations: 13
(See PubMed article )
  Hu / Not Cited
 MA3-916 was used in immunohistochemistry and western blot to examine the expression of isoforms of SR calcium-release channel in the human heart and quantify the corresponding expression changes due to cardiac failure
Circulation. 2001 Jun 5;103(22):2739-44.
"Increased expression of isoform 1 of the sarcoplasmic reticulum Ca(2+)-release channel in failing human heart."
Author(s): Münch G, Bölck B, Sugaru A, Brixius K, Bloch W, Schwinger RH
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
 MA3-916 was used in immunocytochemistry, immunoprecipitation and western blot to study the role of mAKAP and ryanodine receptor in cardiomyocyte nuclear envelope signalling.
J Cell Sci. 2001 Sep;114(Pt 17):3167-76.
"mAKAP and the ryanodine receptor are part of a multi-component signaling complex on the cardiomyocyte nuclear envelope."
Author(s): Kapiloff MS, Jackson N, Airhart N
Number of Citations: 14
(See PubMed article )
  Hu / Not Cited
 MA3-916 was used in western blot to investigate the molecular basis of the aberrant calcium handling during heart failure.
Am J Physiol Heart Circ Physiol. 2007 Mar;292(3):H1607-18.
"Cellular and molecular determinants of altered Ca2+ handling in the failing rabbit heart: primary defects in SR Ca2+ uptake and release mechanisms."
Author(s): Armoundas AA, Rose J, Aggarwal R, Stuyvers BD, O'rourke B, Kass DA, Marbán E, Shorofsky SR, Tomaselli GF, William Balke C
Number of Citations: 1
(See PubMed article )
  Ms / 1:1,000
 MA3-916 was used in western blot to investigate the compensatory mechanisms for the hyperdynamic function of phospholamban-deficient mouse hearts
Circ Res. 1996 Dec;79(6):1064-76.
"Compensatory mechanisms associated with the hyperdynamic function of phospholamban-deficient mouse hearts."
Author(s): Chu G, Luo W, Slack JP, Tilgmann C, Sweet WE, Spindler M, Saupe KW, Boivin GP, Moravec CS, Matlib MA, Grupp IL, Ingwall JS, Kranias EG
Number of Citations: 9
(See PubMed article )
  Ms / Not Cited
 MA3-916 was used in western blot to demonstrate the effect of protamine on the ryanodine receptor and its potential as a ryanodine receptor inhibitor.
Mol Biol Cell. 2000 Jul;11(7):2213-9.
"Reversible block of the calcium release channel/ryanodine receptor by protamine, a heparin antidote."
Author(s): Koulen P, Ehrlich BE
Number of Citations: 4
(See PubMed article )
  Ms / Not Cited
 MA3-916 was used in western blot to demonstrate the vasopressin effect on aquaporin-2 trafficking in the renal collecting duct.
J Biol Chem. 2000 Nov 24;275(47):36839-46.
"Regulation of aquaporin-2 trafficking by vasopressin in the renal collecting duct. Roles of ryanodine-sensitive Ca2+ stores and calmodulin."
Author(s): Chou CL, Yip KP, Michea L, Kador K, Ferraris JD, Wade JB, Knepper MA
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
 MA3-916 was used in western blot to demonstrate the protective effect of nitric oxide on cardiac SL NOSs and sodium/potassium-ATPase against ischemia-induced inactivation.
J Biol Chem. 2003 Oct 24;278(43):41798-803.
"Nitric oxide protects cardiac sarcolemmal membrane enzyme function and ion active transport against ischemia-induced inactivation."
Author(s): Xu KY, Kuppusamy SP, Wang JQ, Li H, Cui H, Dawson TM, Huang PL, Burnett AL, Kuppusamy P, Becker LC
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
 MA3-916 was used in western blot to investigate the role of SC35 in the heart
EMBO J. 2004 Feb 25;23(4):885-96.
"Dilated cardiomyopathy caused by tissue-specific ablation of SC35 in the heart."
Author(s): Ding JH, Xu X, Yang D, Chu PH, Dalton ND, Ye Z, Yeakley JM, Cheng H, Xiao RP, Ross J, Chen J, Fu XD
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
 MA3-916 was used in western blot to conduct proteomic profiling in phospholamban mutant mice.
Proc Natl Acad Sci U S A. 2004 Feb 24;101(8):2241-6.
"Identification of biochemical adaptations in hyper- or hypocontractile hearts from phospholamban mutant mice by expression proteomics."
Author(s): Pan Y, Kislinger T, Gramolini AO, Zvaritch E, Kranias EG, MacLennan DH, Emili A
Number of Citations: 4
(See PubMed article )
  Ms / 1:1,000
 MA3-916 was used in western blot and immunocytochemistry to investigate the differential distribution of inositol (1,4,5)-trisphosphate receptors and calcium signaling in rod bipolar cells.
Invest Ophthalmol Vis Sci. 2005 Jan;46(1):292-8.
"Differentially distributed IP3 receptors and Ca2+ signaling in rod bipolar cells."
Author(s): Koulen P, Wei J, Madry C, Liu J, Nixon E
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
 MA3-916 was used in western blot and immunocytochemistry to study the expression of ryanodine receptors in mouse airway smooth muscle cells.
J Biol Chem. 2005 Jul 15;280(28):26287-94.
"Ryanodine receptors in muscarinic receptor-mediated bronchoconstriction."
Author(s): Du W, Stiber JA, Rosenberg PB, Meissner G, Eu JP
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
 MA3-916 was used in western blot to investigate the important role of junctophilins in neural excitability and plasticity
Proc Natl Acad Sci U S A. 2006 Jul 11;103(28):10811-6.
"Functional uncoupling between Ca2+ release and afterhyperpolarization in mutant hippocampal neurons lacking junctophilins."
Author(s): Moriguchi S, Nishi M, Komazaki S, Sakagami H, Miyazaki T, Masumiya H, Saito SY, Watanabe M, Kondo H, Yawo H, Fukunaga K, Takeshima H
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
 MA3-916 was used in immunoprecipitation and western blot to study the effect of the phosphorylation of ryanodine receptor
Circ Res. 2006 Aug 18;99(4):398-406.
"Ca2+/Calmodulin-dependent protein kinase II phosphorylation of ryanodine receptor does affect calcium sparks in mouse ventricular myocytes."
Author(s): Guo T, Zhang T, Mestril R, Bers DM
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
 MA3-916 was used in western blot to investigate the role of HRCBP in sarcoplasmic reticulum (SR) calcium handling
Mol Cell Biol. 2006 Dec;26(24):9315-26.
"Increased susceptibility to isoproterenol-induced cardiac hypertrophy and impaired weight gain in mice lacking the histidine-rich calcium-binding protein."
Author(s): Jaehnig EJ, Heidt AB, Greene SB, Cornelissen I, Black BL
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
 MA3-916 was used in western blot and immunoprecipitation to investigate the effect of polycystin-2 on ryanodine receptor-dependent calcium signaling.
Proc Natl Acad Sci U S A. 2007 Apr 10;104(15):6454-9.
"Regulation of ryanodine receptor-dependent calcium signaling by polycystin-2."
Author(s): Anyatonwu GI, Estrada M, Tian X, Somlo S, Ehrlich BE
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
 MA3-916 was used in western blot to investigate the structural and functional consequences of the R33Q homozygous mutation in cardiac calsequestrin in a homozygous CASQ2 (R33Q/R33Q) knock in mouse model
Circ Res. 2008 Aug 1;103(3):298-306.
"Unexpected structural and functional consequences of the R33Q homozygous mutation in cardiac calsequestrin: a complex arrhythmogenic cascade in a knock in mouse model."
Author(s): Rizzi N, Liu N, Napolitano C, Nori A, Turcato F, Colombi B, Bicciato S, Arcelli D, Spedito A, Scelsi M, Villani L, Esposito G, Boncompagni S, Protasi F, Volpe P, Priori SG
Number of Citations: 1
(See PubMed article )
  Ms / 1:200
 MA3-916 was used in immunocytochemistry and western blot to identify the function of intracellular calciumchannels on the regulation of specific cellular functions in mouse lacrimal gland acinar cells
Open Ophthalmol J. 2007 Dec 4;1():8-16.
"Identification and functional distribution of intracellular ca channels in mouse lacrimal gland acinar cells."
Author(s): Medina-Ortiz WE, Gregg EV, Brun-Zinkernagel AM, Koulen P
Number of Citations: 1
(See PubMed article )
  Ms / 1:1,000
 MA3-916 was used in western blot to study the phenotype of transgenic rats overexpressing sodium/calcium exchanger .
Am J Physiol Heart Circ Physiol. 2009 Aug;297(2):H590-601.
"Induced overexpression of Na+/Ca2+ exchanger transgene: altered myocyte contractility, [Ca2+]i transients, SR Ca2+ contents, and action potential duration."
Author(s): Wang J, Chan TO, Zhang XQ, Gao E, Song J, Koch WJ, Feldman AM, Cheung JY
Number of Citations: 1
(See PubMed article )
  Ms / 1:1,000
 MA3-916 was used in western blot to investigate the role of ryanodine receptor in the modulation of myocytes induced by NOS1
J Physiol. 2010 Aug 1;588(Pt 15):2905-17.
"Regulation of myocyte contraction via neuronal nitric oxide synthase: role of ryanodine receptor S-nitrosylation."
Author(s): Wang H, Viatchenko-Karpinski S, Sun J, Györke I, Benkusky NA, Kohr MJ, Valdivia HH, Murphy E, Györke S, Ziolo MT
Number of Citations: 1
(See PubMed article )
  Po / Not Cited
 MA3-916 was used in western blot to study the role of ryanodine receptors from sea urchin eggs.
J Physiol. 1998 Jul 1;510 ( Pt 1)():155-64.
"Detection and functional characterization of ryanodine receptors from sea urchin eggs."
Author(s): Lokuta AJ, Darszon A, Beltrán C, Valdivia HH
Number of Citations: 1
(See PubMed article )
  Rb / Not Cited
 MA3-916 was used in immunohistochemistry and western blot to investigate the distribution of sarcolemmal DHPR, RyR and triadin in adult rabbit ventricle and atrium
J Cell Biol. 1995 May;129(3):673-82.
"Immunolocalization of sarcolemmal dihydropyridine receptor and sarcoplasmic reticular triadin and ryanodine receptor in rabbit ventricle and atrium."
Author(s): Carl SL, Felix K, Caswell AH, Brandt NR, Ball WJ Jr, Vaghy PL, Meissner G, Ferguson DG
Number of Citations: 47
(See PubMed article )
  Rb / Not Cited
 MA3-916 was used in ELISA and western blot to investigate the accessibility of RyR1 domains to specific antibodies
J Biol Chem. 1995 May 12;270(19):11338-47.
"Lumenal sites and C terminus accessibility of the skeletal muscle calcium release channel (ryanodine receptor)."
Author(s): Grunwald R, Meissner G
Number of Citations: 14
(See PubMed article )
  Rb / Not Cited
 MA3-916 was used in western blot to study the role of ryanodine receptors from sea urchin eggs.
J Physiol. 1998 Jul 1;510 ( Pt 1)():155-64.
"Detection and functional characterization of ryanodine receptors from sea urchin eggs."
Author(s): Lokuta AJ, Darszon A, Beltrán C, Valdivia HH
Number of Citations: 1
(See PubMed article )
  Rb / Not Cited
 MA3-916 was used in western blot to investigate the expression and function of the cardiac muscle ryanodine receptor calcium release channel in Chinese hamster ovary cells
Biophys J. 1999 Aug;77(2):808-16.
"Expression and functional characterization of the cardiac muscle ryanodine receptor Ca(2+) release channel in Chinese hamster ovary cells."
Author(s): Bhat MB, Hayek SM, Zhao J, Zang W, Takeshima H, Wier WG, Ma J
Number of Citations: 1
(See PubMed article )
  Rb / Not Cited
 MA3-916 was used in western blot to investigate the role of long-chain acylcarnitine on caclium efflux from the sarcoplasmic reticulum
J Cardiovasc Pharmacol. 2000 Jul;36(1):14-21.
"Long-chain acylcarnitine induces Ca2+ efflux from the sarcoplasmic reticulum."
Author(s): Yamada KA, Kanter EM, Newatia A
Number of Citations: 2
(See PubMed article )
  Rb / Not Cited
 MA3-916 was used in immunohistochemistry and western blot to study the localization of DHPR and RyR in rabbit myocarial cells during ontogeny
Am J Physiol Heart Circ Physiol. 2000 Jul;279(1):H202-9.
"Colocalization of dihydropyridine and ryanodine receptors in neonate rabbit heart using confocal microscopy."
Author(s): Sedarat F, Xu L, Moore ED, Tibbits GF
Number of Citations: 8
(See PubMed article )
  Rb / Not Cited
 MA3-916 was used in ELISA and western blot to study the role of a highly conserved sequence of the calcium release channel in ryanodine and caffeine binding and activation.
J Biol Chem. 2001 Aug 24;276(34):31760-71.
"Functional characterization of mutants in the predicted pore region of the rabbit cardiac muscle Ca(2+) release channel (ryanodine receptor isoform 2)."
Author(s): Du GG, Guo X, Khanna VK, MacLennan DH
Number of Citations: 1
(See PubMed article )
  Rb / Not Cited
 MA3-916 was used in western blot to investigate the molecular basis of the aberrant calcium handling during heart failure.
Am J Physiol Heart Circ Physiol. 2007 Mar;292(3):H1607-18.
"Cellular and molecular determinants of altered Ca2+ handling in the failing rabbit heart: primary defects in SR Ca2+ uptake and release mechanisms."
Author(s): Armoundas AA, Rose J, Aggarwal R, Stuyvers BD, O'rourke B, Kass DA, Marbán E, Shorofsky SR, Tomaselli GF, William Balke C
Number of Citations: 1
(See PubMed article )
  Rb / Not Cited
 MA3-916 was used in western blot to suggest that IP3R-dependent calcium release has positive inotropic effects on ECC by facilitating calcium release
Am J Physiol Heart Circ Physiol. 2008 Feb;294(2):H596-604.
"IP3 receptor-dependent Ca2+ release modulates excitation-contraction coupling in rabbit ventricular myocytes."
Author(s): Domeier TL, Zima AV, Maxwell JT, Huke S, Mignery GA, Blatter LA
Number of Citations: 1
(See PubMed article )
  Rt / Not Cited
 MA3-916 was used in western blot to demonstrate that MC possess elements of a novel ADP-ribosyl cyclase signaling pathway regulated by proinflammatory cytokines and steroid superfamily hormones.
Am J Physiol Renal Physiol. 2001 Jul;281(1):F91-F102.
"cADP-ribose/ryanodine channel/Ca2+-release signal transduction pathway in mesangial cells."
Author(s): Yusufi AN, Cheng J, Thompson MA, Dousa TP, Warner GM, Walker HJ, Grande JP
Number of Citations: 1
(See PubMed article )
  Rt / Not Cited
 MA3-916 was used in immunocytochemistry, immunoprecipitation and western blot to study the role of mAKAP and ryanodine receptor in cardiomyocyte nuclear envelope signalling.
J Cell Sci. 2001 Sep;114(Pt 17):3167-76.
"mAKAP and the ryanodine receptor are part of a multi-component signaling complex on the cardiomyocyte nuclear envelope."
Author(s): Kapiloff MS, Jackson N, Airhart N
Number of Citations: 14
(See PubMed article )
  Rt / Not Cited
 MA3-916 was used in western blot to investigate the dysfunction of RyR3 in diabetic rat hearts
Mol Pharmacol. 2001 Dec;60(6):1356-64.
"Ryanodine receptor dysfunction in hearts of streptozotocin-induced diabetic rats."
Author(s): Bidasee KR, Dinçer UD, Besch HR Jr
Number of Citations: 3
(See PubMed article )
  Rt / Not Cited
 MA3-916 was used in western blot to study the effect of chronic diabetes on RyR2 glycation
Diabetes. 2003 Jul;52(7):1825-36.
"Chronic diabetes increases advanced glycation end products on cardiac ryanodine receptors/calcium-release channels."
Author(s): Bidasee KR, Nallani K, Yu Y, Cocklin RR, Zhang Y, Wang M, Dincer UD, Besch HR Jr
Number of Citations: 6
(See PubMed article )
  Rt / Not Cited
 MA3-916 was used in western blot to study the functional properties of TRD in myocardial intracellular Ca handling
Circ Res. 2005 Apr 1;96(6):651-8.
"Triadin overexpression stimulates excitation-contraction coupling and increases predisposition to cellular arrhythmia in cardiac myocytes."
Author(s): Terentyev D, Cala SE, Houle TD, Viatchenko-Karpinski S, Gyorke I, Terentyeva R, Williams SC, Gyorke S
Number of Citations: 1
(See PubMed article )
  Rt / 1:100
 MA3-916 was used in western blot and immunocytochemistry to study excitation-contraction coupling mechanisms in striated and smooth muscles.
J Biol Chem. 2006 Oct 6;281(40):30143-51.
"Excitation-contraction coupling in airway smooth muscle."
Author(s): Du W, McMahon TJ, Zhang ZS, Stiber JA, Meissner G, Eu JP
Number of Citations: 1
(See PubMed article )
  Rt / Not Cited
 MA3-916 was used in immunohistochemistry and western blot to investigate the TRPC signalplex in rat ventricular myocytes
Am J Physiol Heart Circ Physiol. 2007 Feb;292(2):H874-83.
"TRPC3 channels colocalize with Na+/Ca2+ exchanger and Na+ pump in axial component of transverse-axial tubular system of rat ventricle."
Author(s): Goel M, Zuo CD, Sinkins WG, Schilling WP
Number of Citations: 1
(See PubMed article )
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