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Ryanodine Receptor Monoclonal Antibody (34C)

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Ryanodine Receptor Antibody for IF, IHC (F), IP, Western blot

>> See 2 other antibodies for RYR1 (RyR)

Synonyms:

RyR

Entrez Gene ID:

(Human) 6261, (Murine) 20190, (Rat) 686059

UniProt ID:

P21817

Details

Host / Isotype:	Mouse / IgG1
Class:	Monoclonal
Type:	Antibody
Clone:	34C
Species Reactivity:	Human (Hu) Murine (Ms) Mustelid (Md) Rat (Rt) Canine (Ca) Rabbit (Rb) Non-human primate (Nhp) Bovine (Bv) Ovine (Ov) Amphibian (Am) Fish (Fs)
Immunogen:	Partially purified chicken pectoral muscle ryanodine receptor.

Ordering Information
Pierce Ryanodine Receptor Monoclonal Antibody (34C)
Product Number	Pkg. Size	Price	Purchase

MA3-925	100 µl	$357.00	Qty:

Storage:	-20º C, Avoid Freeze/Thaw Cycles
Form:	100 µl of purified IgG1 Antibody (1mg/ml) containing 0.05% sodium azide.

Applications	Dilution *

Immunofluorescence (IF)	Assay dependent
Western Blot (WB)	1:5,000
Immunoprecipitation (IP)	Assay dependent
Immunohistochemistry (Frozen) (IHC (F))	1:1,000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Product Specific Information

MA3-925 detects ryanodine receptor (RyR)-1 and RyR-2 isoforms in amphibian, bovine, canine, fish, human, non-human primate, mouse, rabbit, mink, rat and sheep tissues. In chickens this antibody detects the alpha, beta and cardiac isoforms. This antibody also detects RyR-3 in mouse cells. In frog, MA3-925 detects the alpha and beta isoforms and in fish it detects the alpha isoform.

MA3-925 has been successfully used in Western blot, immunohistochemistry, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects a 565 kDa protein representing RyR from rat skeletal muscle extracts. In non-mammalian vertebrates, a doublet is seen at 565 kDa representing the alpha and beta isoforms of the receptor. Immunohistochemical staining of RyR in chicken brain with MA3-925 results in intense staining of cerebellar Purkinje neurons.

The MA3-925 antigen is partially purified chicken pectoral muscle ryanodine receptor.

General Information

The ryanodine receptor (RyR) is the channel responsible for calcium release from muscle cell sarcoplasmic reticulum (SR) and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains. The remainder of the protein, termed the "foot" region, is located in the cytoplasm between the transverse tubule and the SR.

Mammalian RyR isoforms are the product of three different genes: RyR-1 is expressed predominantly in skeletal muscle and areas of the brain; RyR-2 is expressed predominantly in heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain; and RyR-3 is found in smooth muscle and the brain (striatum, thalamus and hippocampus). In non-mammalian vertebrates, the RyR isoforms are termed alpha, beta and cardiac which correlate loosely to the mammalian RyR-1, RyR-3 and RyR-2 isoforms respectively.

PubMed References:

BLOCK (1)
ICC (9)
IHC (6)
IP (11)
WB (41)

Blocking Assay
	Species / Dilution	Summary
	Rb / Not Cited	MA3-925 was used in blocking/activating experiment, immunoprecipitation and western blot to investigate the mechanism for the interaction between RyR and triadin J Biol Chem. 1994 Dec 30;269(52):33028-34. "Molecular interaction between ryanodine receptor and glycoprotein triadin involves redox cycling of functionally important hyperreactive sulfhydryls." Author(s): Liu G, Pessah IN Number of Citations: 8 (See PubMed article )

Immunocytochemistry
	Species / Dilution	Summary
	Ck / 1:100	MA3-925 was used in immunocytochemistry to demonstrate the effect of ryanodine receptor 1 on muscle fiber type specification. J Cell Sci. 2004 Dec 1;117(Pt 25):6175-83. "Inhibition of ryanodine receptor 1 in fast skeletal muscle fibers induces a fast-to-slow muscle fiber type transition." Author(s): Jordan T, Jiang H, Li H, DiMario JX Number of Citations: 1 (See PubMed article )
	Hu / 1:200	MA3-925 was used in immunocytochemistry to suggest a role for metabotropic glutamate receptor mGlu5 in intranuclear calcium signaling pathways. J Biol Chem. 2003 Jul 25;278(30):28210-9. "Activation of metabotropic glutamate receptor mGlu5 on nuclear membranes mediates intranuclear Ca2+ changes in heterologous cell types and neurons." Author(s): O'Malley KL, Jong YJ, Gonchar Y, Burkhalter A, Romano C Number of Citations: 1 (See PubMed article )
	Hu / Not Cited	MA3-925 was used in immunocytochemistry to investigate the role of RyR2 in beta-cell survival in vitro and its mechanism . J Biol Chem. 2004 Jun 4;279(23):24794-802. "RyR2 and calpain-10 delineate a novel apoptosis pathway in pancreatic islets." Author(s): Johnson JD, Han Z, Otani K, Ye H, Zhang Y, Wu H, Horikawa Y, Misler S, Bell GI, Polonsky KS Number of Citations: 1 (See PubMed article )
	Hu / 1:100	MA3-925 was used in western blot and immunocytochemistry to investigate the roles of local calcium derangements in the specific cellular phenotypes of malignant hyperthermia and central core disease mutations. J Biol Chem. 2005 Apr 15;280(15):15380-9. "Ca2+ signaling in HEK-293 and skeletal muscle cells expressing recombinant ryanodine receptors harboring malignant hyperthermia and central core disease mutations." Author(s): Brini M, Manni S, Pierobon N, Du GG, Sharma P, MacLennan DH, Carafoli E Number of Citations: 1 (See PubMed article )
	Ms / 1:1,000	MA3-925 was used in western blot, immunoprecipitation and immunocytochemistry to study the role of presenilin 1 mutations in Alzheimer disease pathogenesis . J Biol Chem. 2000 Jun 16;275(24):18195-200. "Presenilin-1 mutations increase levels of ryanodine receptors and calcium release in PC12 cells and cortical neurons." Author(s): Chan SL, Mayne M, Holden CP, Geiger JD, Mattson MP Number of Citations: 1 (See PubMed article )
	Ms / 1:800	MA3-925 was used in immunocytochemistry to study the organization of dopaminergic interplexiform processes in the outer layer of rodent retinas and possible corresponding functions J Comp Neurol. 2008 Sep 10;510(2):158-74. "Anatomical and neurochemical characterization of dopaminergic interplexiform processes in mouse and rat retinas." Author(s): Witkovsky P, Gábriel R, Krizaj D Number of Citations: 1 (See PubMed article )
	Ms / 1:100	MA3-925 was used in immunocytochemistry and western blot to identify the function of intracellular calciumchannels on the regulation of specific cellular functions in mouse lacrimal gland acinar cells Open Ophthalmol J. 2007 Dec 4;1():8-16. "Identification and functional distribution of intracellular ca channels in mouse lacrimal gland acinar cells." Author(s): Medina-Ortiz WE, Gregg EV, Brun-Zinkernagel AM, Koulen P Number of Citations: 1 (See PubMed article )
	Po / 1:500	MA3-925 was used in immunocytochemistry to investigate the effects of calcium release from intracellular stores on somatodendritic DA release in SNc J Neurosci. 2009 May 20;29(20):6568-79. "Mobilization of calcium from intracellular stores facilitates somatodendritic dopamine release." Author(s): Patel JC, Witkovsky P, Avshalumov MV, Rice ME Number of Citations: 1 (See PubMed article )
	Rb / Not Cited	MA3-925 was used in immunocytochemistry to investigate the translational diffusion of globular proteins in the cytoplasm of cultured muscle cells Biophys J. 2000 Feb;78(2):901-7. "Translational diffusion of globular proteins in the cytoplasm of cultured muscle cells." Author(s): Arrio-Dupont M, Foucault G, Vacher M, Devaux PF, Cribier S Number of Citations: 1 (See PubMed article )

Immunohistochemistry
	Species / Dilution	Summary
	Ck / Not Cited	MA3-925 was used in immunohistochemistry, immunoprecipitation and western blot to investigate the distribution of InsP3 and RyR receptors in avian cerebellar Purkinje neurons J Cell Biol. 1991 Jun;113(5):1145-57. "Ryanodine and inositol trisphosphate receptors coexist in avian cerebellar Purkinje neurons." Author(s): Walton PD, Airey JA, Sutko JL, Beck CF, Mignery GA, Südhof TC, Deerinck TJ, Ellisman MH Number of Citations: 27 (See PubMed article )
	Hu / 1:100	MA3-925 was used in immunohistochemistry to study the localization and function of the nitric oxide synthase in heart. Proc Natl Acad Sci U S A. 1999 Jan 19;96(2):657-62. "Nitric oxide synthase in cardiac sarcoplasmic reticulum." Author(s): Xu KY, Huso DL, Dawson TM, Bredt DS, Becker LC Number of Citations: 35 (See PubMed article )
	Ms / 1:2	MA3-925 was used in immunohistochemistry to investigate the calcium release apparatus in skeletal muscle J Cell Biol. 1998 Feb 23;140(4):831-42. "Role of ryanodine receptors in the assembly of calcium release units in skeletal muscle." Author(s): Protasi F, Franzini-Armstrong C, Allen PD Number of Citations: 28 (See PubMed article )
	Ms / 1:100	MA3-925 was used in immunohistochemistry to locate neuregulin receptor ErbB2 at T-tubule in cardiac and skeletal muscle J Histochem Cytochem. 2005 Jan;53(1):87-91. "Neuregulin receptor ErbB2 localization at T-tubule in cardiac and skeletal muscle." Author(s): Ueda H, Oikawa A, Nakamura A, Terasawa F, Kawagishi K, Moriizumi T Number of Citations: 1 (See PubMed article )
	Rt / 1:150	MA3-925 was used in immunohistochemistry to study the cellular distribution of RyR in rat cardiac hypertrophy Circulation. 1998 Dec 1;98(22):2477-86. "Cellular distribution of Ca2+ pumps and Ca2+ release channels in rat cardiac hypertrophy induced by aortic stenosis." Author(s): Anger M, Lompré AM, Vallot O, Marotte F, Rappaport L, Samuel JL Number of Citations: 0 (See PubMed article )
	Rt / 1:500	MA3-925 was used in immunohistochemistry to investigate the role of the tumor necrosis factor during the central vagal afferent signaling process. J Neurosci. 2006 Dec 6;26(49):12642-6. "Tumor necrosis factor potentiates central vagal afferent signaling by modulating ryanodine channels." Author(s): Rogers RC, Van Meter MJ, Hermann GE Number of Citations: 1 (See PubMed article )

Immunoprecipitation
	Species / Dilution	Summary
	Am / Not Cited	MA3-925 was used in immunoprecipitation and western blot to investigate triad junctional foot protein isoforms in mammalian and nonmammalian vertebrate skeletal muscles Biophys J. 1991 Jun;59(6):1153-63. "Nonmammalian vertebrate skeletal muscles express two triad junctional foot protein isoforms." Author(s): Olivares EB, Tanksley SJ, Airey JA, Beck CF, Ouyang Y, Deerinck TJ, Ellisman MH, Sutko JL Number of Citations: 1 (See PubMed article )
	Ck / Not Cited	MA3-925 was used in immunohistochemistry, immunoprecipitation and western blot to investigate the distribution of InsP3 and RyR receptors in avian cerebellar Purkinje neurons J Cell Biol. 1991 Jun;113(5):1145-57. "Ryanodine and inositol trisphosphate receptors coexist in avian cerebellar Purkinje neurons." Author(s): Walton PD, Airey JA, Sutko JL, Beck CF, Mignery GA, Südhof TC, Deerinck TJ, Ellisman MH Number of Citations: 27 (See PubMed article )
	Ck / Not Cited	MA3-925 was used in immunoprecipitation and western blot to investigate triad junctional foot protein isoforms in mammalian and nonmammalian vertebrate skeletal muscles Biophys J. 1991 Jun;59(6):1153-63. "Nonmammalian vertebrate skeletal muscles express two triad junctional foot protein isoforms." Author(s): Olivares EB, Tanksley SJ, Airey JA, Beck CF, Ouyang Y, Deerinck TJ, Ellisman MH, Sutko JL Number of Citations: 1 (See PubMed article )
	Ck / Not Cited	MA3-925 was used in immunoprecipitation and western blot to characterize two triad junctional foot protein isoforms and investigate their distribution in mature chicken fast twitch skeletal muscle J Biol Chem. 1990 Aug 25;265(24):14187-94. "Identification and localization of two triad junctional foot protein isoforms in mature avian fast twitch skeletal muscle." Author(s): Airey JA, Beck CF, Murakami K, Tanksley SJ, Deerinck TJ, Ellisman MH, Sutko JL Number of Citations: 36 (See PubMed article )
	Ck / Not Cited	MA3-925 was used in immunoprecipitation and western blot to investigate the distribution of ryanodine receptor isoforms in chicken skeletal and cardiac muscles Biochemistry. 1993 Jun 8;32(22):5739-45. "Three ryanodine receptor isoforms exist in avian striated muscles." Author(s): Airey JA, Grinsell MM, Jones LR, Sutko JL, Witcher D Number of Citations: 7 (See PubMed article )
	Ms / Not Cited	MA3-925 was used in western blot, immunoprecipitation and immunocytochemistry to study the role of presenilin 1 mutations in Alzheimer disease pathogenesis . J Biol Chem. 2000 Jun 16;275(24):18195-200. "Presenilin-1 mutations increase levels of ryanodine receptors and calcium release in PC12 cells and cortical neurons." Author(s): Chan SL, Mayne M, Holden CP, Geiger JD, Mattson MP Number of Citations: 1 (See PubMed article )
	Ms / Not Cited	MA3-925 was used in immunoprecipitation to study the RyR phosphorylation in PLB-KO mouse ventricular myocytes Circ Res. 2002 Feb 22;90(3):309-16. "Protein kinase A phosphorylation of the ryanodine receptor does not affect calcium sparks in mouse ventricular myocytes." Author(s): Li Y, Kranias EG, Mignery GA, Bers DM Number of Citations: 29 (See PubMed article )
	Rb / Not Cited	MA3-925 was used in blocking/activating experiment, immunoprecipitation and western blot to investigate the mechanism for the interaction between RyR and triadin J Biol Chem. 1994 Dec 30;269(52):33028-34. "Molecular interaction between ryanodine receptor and glycoprotein triadin involves redox cycling of functionally important hyperreactive sulfhydryls." Author(s): Liu G, Pessah IN Number of Citations: 8 (See PubMed article )
	Rb / 1:50	MA3-925 was used in immunoprecipitation to investigate the function of mastoparan in heavy fraction of SR of rabbit skeletal muscles Mol Pharmacol. 2000 Jun;57(6):1235-42. "Identification of a 97-kDa mastoparan-binding protein involving in Ca(2+) release from skeletal muscle sarcoplasmic reticulum." Author(s): Hirata Y, Nakahata N, Ohizumi Y Number of Citations: 3 (See PubMed article )
	Rt / Not Cited	MA3-925 was used in immunoprecipitation and western blot to characterize cardiac type 2 inositol 1,4,5-trisphosphate receptor. J Biol Chem. 2005 Apr 22;280(16):15912-20. "Cardiac type 2 inositol 1,4,5-trisphosphate receptor: interaction and modulation by calcium/calmodulin-dependent protein kinase II." Author(s): Bare DJ, Kettlun CS, Liang M, Bers DM, Mignery GA Number of Citations: 1 (See PubMed article )
	Rt / Not Cited	MA3-925 was used in immunoprecipitation and western blot to investigate the phosphorylation of Ryanodine receptor (RyR) in rat cardiomyocytes Biochem Biophys Res Commun. 2008 Nov 7;376(1):80-5. "Ryanodine receptor phosphorylation at Serine 2030, 2808 and 2814 in rat cardiomyocytes." Author(s): Huke S, Bers DM Number of Citations: 1 (See PubMed article )

Western Blot
	Species / Dilution	Summary
	Am / Not Cited	MA3-925 was used in immunoprecipitation and western blot to investigate triad junctional foot protein isoforms in mammalian and nonmammalian vertebrate skeletal muscles Biophys J. 1991 Jun;59(6):1153-63. "Nonmammalian vertebrate skeletal muscles express two triad junctional foot protein isoforms." Author(s): Olivares EB, Tanksley SJ, Airey JA, Beck CF, Ouyang Y, Deerinck TJ, Ellisman MH, Sutko JL Number of Citations: 1 (See PubMed article )
	Bv / Not Cited	MA3-925 was used in western blot to study how IL-4 inhibits calcium transients in bovine trachealis cells FASEB J. 2006 Jan;20(1):154-6. "IL-4 inhibits calcium transients in bovine trachealis cells by a ryanodine receptor-dependent mechanism." Author(s): Ethier MF, Madison JM Number of Citations: 1 (See PubMed article )
	Ca / 1:5,000	MA3-925 was used in western blot to investigate the role of RyRs, SERCA2a, and phospholamban (PLB) in canine and human heart failure Circ Res. 2002 Nov 29;91(11):1015-22. "Abnormal Ca2+ release, but normal ryanodine receptors, in canine and human heart failure." Author(s): Jiang MT, Lokuta AJ, Farrell EF, Wolff MR, Haworth RA, Valdivia HH Number of Citations: 34 (See PubMed article )
	Ck / Not Cited	MA3-925 was used in immunohistochemistry, immunoprecipitation and western blot to investigate the distribution of InsP3 and RyR receptors in avian cerebellar Purkinje neurons J Cell Biol. 1991 Jun;113(5):1145-57. "Ryanodine and inositol trisphosphate receptors coexist in avian cerebellar Purkinje neurons." Author(s): Walton PD, Airey JA, Sutko JL, Beck CF, Mignery GA, Südhof TC, Deerinck TJ, Ellisman MH Number of Citations: 27 (See PubMed article )
	Ck / Not Cited	MA3-925 was used in immunoprecipitation and western blot to characterize two triad junctional foot protein isoforms and investigate their distribution in mature chicken fast twitch skeletal muscle J Biol Chem. 1990 Aug 25;265(24):14187-94. "Identification and localization of two triad junctional foot protein isoforms in mature avian fast twitch skeletal muscle." Author(s): Airey JA, Beck CF, Murakami K, Tanksley SJ, Deerinck TJ, Ellisman MH, Sutko JL Number of Citations: 36 (See PubMed article )
	Ck / Not Cited	MA3-925 was used in immunoprecipitation and western blot to investigate the distribution of ryanodine receptor isoforms in chicken skeletal and cardiac muscles Biochemistry. 1993 Jun 8;32(22):5739-45. "Three ryanodine receptor isoforms exist in avian striated muscles." Author(s): Airey JA, Grinsell MM, Jones LR, Sutko JL, Witcher D Number of Citations: 7 (See PubMed article )
	Hu / Not Cited	MA3-925 was used in western blot to study the role of skeletal muscle type RYR in calcium signaling in human B lymphocytes J Biol Chem. 1999 Feb 26;274(9):5995-6002. "Skeletal muscle type ryanodine receptor is involved in calcium signaling in human B lymphocytes." Author(s): Sei Y, Gallagher KL, Basile AS Number of Citations: 7 (See PubMed article )
	Hu / 1:5,000	MA3-925 was used in western blot to study how intermittent sprint training affects sarcoplasmic reticulum functions Am J Physiol Regul Integr Comp Physiol. 2000 Jul;279(1):R152-60. "Enhanced sarcoplasmic reticulum Ca(2+) release following intermittent sprint training." Author(s): Ortenblad N, Lunde PK, Levin K, Andersen JL, Pedersen PK Number of Citations: 1 (See PubMed article )
	Hu / Not Cited	MA3-925 was used in western blot to study the effects of increase in L-type calcium channels density on calcium signaling in cardiac myocytes overexpressing the alpha1 subunit of LCC Circ Res. 2002 Feb 8;90(2):174-81. "Ca(2+) signaling in cardiac myocytes overexpressing the alpha(1) subunit of L-type Ca(2+) channel." Author(s): Song LS, Guia A, Muth JN, Rubio M, Wang SQ, Xiao RP, Josephson IR, Lakatta EG, Schwartz A, Cheng H Number of Citations: 5 (See PubMed article )
	Hu / Not Cited	MA3-925 was used in western blot to investigate the function of three ryanodine receptor isoforms during the formation of the functional heteromeric channels. J Biol Chem. 2002 Nov 1;277(44):41778-85. "Isoform-dependent formation of heteromeric Ca2+ release channels (ryanodine receptors)." Author(s): Xiao B, Masumiya H, Jiang D, Wang R, Sei Y, Zhang L, Murayama T, Ogawa Y, Lai FA, Wagenknecht T, Chen SR Number of Citations: 1 (See PubMed article )
	Hu / 1:5,000	MA3-925 was used in western blot to investigate the role of RyRs, SERCA2a, and phospholamban (PLB) in canine and human heart failure Circ Res. 2002 Nov 29;91(11):1015-22. "Abnormal Ca2+ release, but normal ryanodine receptors, in canine and human heart failure." Author(s): Jiang MT, Lokuta AJ, Farrell EF, Wolff MR, Haworth RA, Valdivia HH Number of Citations: 34 (See PubMed article )
	Hu / Not Cited	MA3-925 was used in western blot to study the effect of FKBP12 on the activity of the type-3 ryanodine receptor. J Cell Sci. 2004 Mar 1;117(Pt 7):1129-37. "The 12 kDa FK506-binding protein, FKBP12, modulates the Ca(2+)-flux properties of the type-3 ryanodine receptor." Author(s): Van Acker K, Bultynck G, Rossi D, Sorrentino V, Boens N, Missiaen L, De Smedt H, Parys JB, Callewaert G Number of Citations: 1 (See PubMed article )
	Hu / Not Cited	MA3-925 was used in western blot and immunocytochemistry to investigate the roles of local calcium derangements in the specific cellular phenotypes of malignant hyperthermia and central core disease mutations. J Biol Chem. 2005 Apr 15;280(15):15380-9. "Ca2+ signaling in HEK-293 and skeletal muscle cells expressing recombinant ryanodine receptors harboring malignant hyperthermia and central core disease mutations." Author(s): Brini M, Manni S, Pierobon N, Du GG, Sharma P, MacLennan DH, Carafoli E Number of Citations: 1 (See PubMed article )
	Hu / Not Cited	MA3-925 was used in western blot to investigate the association of mutant ATX3 with the type 1 inositol 1,4,5-trisphosphate receptor and its effect on calcium signalling in spinocerebellar ataxia type 3. J Neurosci. 2008 Nov 26;28(48):12713-24. "Deranged calcium signaling and neurodegeneration in spinocerebellar ataxia type 3." Author(s): Chen X, Tang TS, Tu H, Nelson O, Pook M, Hammer R, Nukina N, Bezprozvanny I Number of Citations: 1 (See PubMed article )
	Hu / Not Cited	MA3-925 was used in western blot to investigate the gene expression of calcium-handling proteins in Tako-Tsubo cardiomyopathy Eur Heart J. 2009 Sep;30(17):2155-64. "Abnormalities in intracellular Ca2+ regulation contribute to the pathomechanism of Tako-Tsubo cardiomyopathy." Author(s): Nef HM, Möllmann H, Troidl C, Kostin S, Voss S, Hilpert P, Behrens CB, Rolf A, Rixe J, Weber M, Hamm CW, Elsässer A Number of Citations: 1 (See PubMed article )
	Ms / 1:5,000	MA3-925 was used in western blot to investigate the role of thyroid hormone receptor (TR)-alpha1 and -beta on skeletal muscle function Am J Physiol Regul Integr Comp Physiol. 2000 Mar;278(3):R598-603. "Isometric force and endurance in soleus muscle of thyroid hormone receptor-alpha(1)- or -beta-deficient mice." Author(s): Johansson C, Lännergren J, Lunde PK, Vennström B, Thorén P, Westerblad H Number of Citations: 2 (See PubMed article )
	Ms / Not Cited	MA3-925 was used in western blot, immunoprecipitation and immunocytochemistry to study the role of presenilin 1 mutations in Alzheimer disease pathogenesis . J Biol Chem. 2000 Jun 16;275(24):18195-200. "Presenilin-1 mutations increase levels of ryanodine receptors and calcium release in PC12 cells and cortical neurons." Author(s): Chan SL, Mayne M, Holden CP, Geiger JD, Mattson MP Number of Citations: 1 (See PubMed article )
	Ms / Not Cited	MA3-925 was used in western blot to study the mechanism for calcium activation of the mouse cardiac ryanodine receptor. J Gen Physiol. 2001 Jul;118(1):33-44. "Molecular basis of Ca(2)+ activation of the mouse cardiac Ca(2)+ release channel (ryanodine receptor)." Author(s): Li P, Chen SR Number of Citations: 18 (See PubMed article )
	Ms / Not Cited	MA3-925 was used in western blot to study the effects of increase in L-type calcium channels density on calcium signaling in cardiac myocytes overexpressing the alpha1 subunit of LCC Circ Res. 2002 Feb 8;90(2):174-81. "Ca(2+) signaling in cardiac myocytes overexpressing the alpha(1) subunit of L-type Ca(2+) channel." Author(s): Song LS, Guia A, Muth JN, Rubio M, Wang SQ, Xiao RP, Josephson IR, Lakatta EG, Schwartz A, Cheng H Number of Citations: 5 (See PubMed article )
	Ms / Not Cited	MA3-925 was used in western blot to study junctin's functions in heart FASEB J. 2002 Aug;16(10):1310-2. "Cardiac remodeling and atrial fibrillation in transgenic mice overexpressing junctin." Author(s): Hong CS, Cho MC, Kwak YG, Song CH, Lee YH, Lim JS, Kwon YK, Chae SW, Kim DH Number of Citations: 1 (See PubMed article )
	Ms / 1:1,000	MA3-925 was used in western blot to study the regulation of NFAT functions through neuromuscular stimulation in rodent skeletal muscles. Proc Natl Acad Sci U S A. 2004 Jun 22;101(25):9387-92. "TRPC3 channels confer cellular memory of recent neuromuscular activity." Author(s): Rosenberg P, Hawkins A, Stiber J, Shelton JM, Hutcheson K, Bassel-Duby R, Shin DM, Yan Z, Williams RS Number of Citations: 1 (See PubMed article )
	Ms / Not Cited	MA3-925 was used in western blot to investigate the important role of junctophilins in neural excitability and plasticity Proc Natl Acad Sci U S A. 2006 Jul 11;103(28):10811-6. "Functional uncoupling between Ca2+ release and afterhyperpolarization in mutant hippocampal neurons lacking junctophilins." Author(s): Moriguchi S, Nishi M, Komazaki S, Sakagami H, Miyazaki T, Masumiya H, Saito SY, Watanabe M, Kondo H, Yawo H, Fukunaga K, Takeshima H Number of Citations: 1 (See PubMed article )
	Ms / 1:100	MA3-925 was used in immunocytochemistry and western blot to identify the function of intracellular calciumchannels on the regulation of specific cellular functions in mouse lacrimal gland acinar cells Open Ophthalmol J. 2007 Dec 4;1():8-16. "Identification and functional distribution of intracellular ca channels in mouse lacrimal gland acinar cells." Author(s): Medina-Ortiz WE, Gregg EV, Brun-Zinkernagel AM, Koulen P Number of Citations: 1 (See PubMed article )
	Ms / Not Cited	MA3-925 was used in western blot to demonstrate that presenilins play an important role in neuronal ER calcium homeostasis J Neurosci. 2010 Jun 23;30(25):8566-80. "Role of presenilins in neuronal calcium homeostasis." Author(s): Zhang H, Sun S, Herreman A, De Strooper B, Bezprozvanny I Number of Citations: 1 (See PubMed article )
	Po / 1:1,1000	MA3-925 was used in western blot to characterize the channels formed by a member of the transient receptor potential family of ion channels polycystin-2. J Biol Chem. 2005 Aug 19;280(33):29488-93. "Organic cation permeation through the channel formed by polycystin-2." Author(s): Anyatonwu GI, Ehrlich BE Number of Citations: 1 (See PubMed article )
	Rb / Not Cited	MA3-925 was used in blocking/activating experiment, immunoprecipitation and western blot to investigate the mechanism for the interaction between RyR and triadin J Biol Chem. 1994 Dec 30;269(52):33028-34. "Molecular interaction between ryanodine receptor and glycoprotein triadin involves redox cycling of functionally important hyperreactive sulfhydryls." Author(s): Liu G, Pessah IN Number of Citations: 8 (See PubMed article )
	Rb / Not Cited	MA3-925 was used in western blot to investigate the major tryptic fragments of RyR and their relationships with the insoluble membrane fraction and sedimentation behavior J Biol Chem. 1993 Oct 25;268(30):22642-9. "Positioning of major tryptic fragments in the Ca2+ release channel (ryanodine receptor) resulting from partial digestion of rabbit skeletal muscle sarcoplasmic reticulum." Author(s): Chen SR, Airey JA, MacLennan DH Number of Citations: 5 (See PubMed article )
	Rb / 1:5,000	MA3-925 was used in western blot to study the structure and function of type 3 ryanodine receptor (RyR3) in rabbit diaphragm J Biol Chem. 1999 Jun 11;274(24):17297-308. "Further characterization of the type 3 ryanodine receptor (RyR3) purified from rabbit diaphragm." Author(s): Murayama T, Oba T, Katayama E, Oyamada H, Oguchi K, Kobayashi M, Otsuka K, Ogawa Y Number of Citations: 12 (See PubMed article )
	Rb / 1:1,000	MA3-925 was used in western blot to investigate the role of long-chain acylcarnitine on caclium efflux from the sarcoplasmic reticulum J Cardiovasc Pharmacol. 2000 Jul;36(1):14-21. "Long-chain acylcarnitine induces Ca2+ efflux from the sarcoplasmic reticulum." Author(s): Yamada KA, Kanter EM, Newatia A Number of Citations: 2 (See PubMed article )
	Rb / Not Cited	MA3-925 was used in western blot to investigate the Three-dimensional structure of the recombinant type 3 ryanodine receptor Proc Natl Acad Sci U S A. 2001 May 22;98(11):6104-9. "Three-dimensional reconstruction of the recombinant type 3 ryanodine receptor and localization of its amino terminus." Author(s): Liu Z, Zhang J, Sharma MR, Li P, Chen SR, Wagenknecht T Number of Citations: 1 (See PubMed article )
	Rb / Not Cited	MA3-925 was used in western blot to investigate the effect of S-glutathionylation and S-nitrosylation on the modulation of RyR1 channels by Mg2+ and calcium. J Biol Chem. 2003 Oct 31;278(44):42927-35. "S-glutathionylation decreases Mg2+ inhibition and S-nitrosylation enhances Ca2+ activation of RyR1 channels." Author(s): Aracena P, Sánchez G, Donoso P, Hamilton SL, Hidalgo C Number of Citations: 1 (See PubMed article )
	Rt / 1:2,500	MA3-925 was used in western blot to investigate the possible relationship between the restoration of the SR calcium-ATPase through adenoviral gene transfer in the aging heart and the normalization of diastolic function in vivo Circulation. 2000 Feb 22;101(7):790-6. "Restoration of diastolic function in senescent rat hearts through adenoviral gene transfer of sarcoplasmic reticulum Ca(2+)-ATPase." Author(s): Schmidt U, del Monte F, Miyamoto MI, Matsui T, Gwathmey JK, Rosenzweig A, Hajjar RJ Number of Citations: 10 (See PubMed article )
	Rt / Not Cited	MA3-925 was used in western blot to demonstrate that MC possess elements of a novel ADP-ribosyl cyclase signaling pathway regulated by proinflammatory cytokines and steroid superfamily hormones. Am J Physiol Renal Physiol. 2001 Jul;281(1):F91-F102. "cADP-ribose/ryanodine channel/Ca2+-release signal transduction pathway in mesangial cells." Author(s): Yusufi AN, Cheng J, Thompson MA, Dousa TP, Warner GM, Walker HJ, Grande JP Number of Citations: 1 (See PubMed article )
	Rt / 1:5,000	MA3-925 was used in western blot to study the contraction and intracellular calcium handling in rat skeletal muscle Circ Res. 2001 Jun 22;88(12):1299-305. "Contraction and intracellular Ca(2+) handling in isolated skeletal muscle of rats with congestive heart failure." Author(s): Lunde PK, Dahlstedt AJ, Bruton JD, Lännergren J, Thorén P, Sejersted OM, Westerblad H Number of Citations: 11 (See PubMed article )
	Rt / 1:700	MA3-925 was used in western blot to study the possible contribution of defects in intracellular calcium signaling cardiomyopathy in streptozotocin-induced diabetic rats Am J Physiol Heart Circ Physiol. 2002 Oct;283(4):H1398-408. "Defective intracellular Ca(2+) signaling contributes to cardiomyopathy in Type 1 diabetic rats." Author(s): Choi KM, Zhong Y, Hoit BD, Grupp IL, Hahn H, Dilly KW, Guatimosim S, Lederer WJ, Matlib MA Number of Citations: 1 (See PubMed article )
	Rt / 1:3,000	MA3-925 was used in western blot to investigate the relationship between ryanodine receptor calcium release and impaired myocardial function FASEB J. 2003 Aug;17(11):1517-9. "Defects in ryanodine receptor calcium release in skeletal muscle from post-myocardial infarct rats." Author(s): Ward CW, Reiken S, Marks AR, Marty I, Vassort G, Lacampagne A Number of Citations: 1 (See PubMed article )
	Rt / Not Cited	MA3-925 was used in western blot to investigaet the ability of S100A2 gene transfer to rescue failing myocardium J Clin Invest. 2004 Dec;114(11):1550-63. "Cardiac adenoviral S100A1 gene delivery rescues failing myocardium." Author(s): Most P, Pleger ST, Völkers M, Heidt B, Boerries M, Weichenhan D, Löffler E, Janssen PM, Eckhart AD, Martini J, Williams ML, Katus HA, Remppis A, Koch WJ Number of Citations: 1 (See PubMed article )
	Rt / 1:500	MA3-925 was used in western blot to investigate the effects of mechanical unloading on cardiomyocytes from doxorubicin-induced cardiomyopathy J Am Coll Cardiol. 2004 Dec 7;44(11):2239-46. "Mechanical unloading improves intracellular Ca2+ regulation in rats with doxorubicin-induced cardiomyopathy." Author(s): Takaseya T, Ishimatsu M, Tayama E, Nishi A, Akasu T, Aoyagi S Number of Citations: 1 (See PubMed article )
	Rt / Not Cited	MA3-925 was used in immunoprecipitation and western blot to characterize cardiac type 2 inositol 1,4,5-trisphosphate receptor. J Biol Chem. 2005 Apr 22;280(16):15912-20. "Cardiac type 2 inositol 1,4,5-trisphosphate receptor: interaction and modulation by calcium/calmodulin-dependent protein kinase II." Author(s): Bare DJ, Kettlun CS, Liang M, Bers DM, Mignery GA Number of Citations: 1 (See PubMed article )
	Rt / 1:3,330	MA3-925 was used in western blot to investigate the role of HSP70 on calcium homeostasis in rat ventricular myocytes subjected to simulated ischemia Am J Physiol Cell Physiol. 2006 Feb;290(2):C583-91. "Further study on the role of HSP70 on Ca2+ homeostasis in rat ventricular myocytes subjected to simulated ischemia." Author(s): Liu J, Kam KW, Borchert GH, Kravtsov GM, Ballard HJ, Wong TM Number of Citations: 1 (See PubMed article )
	Rt / Not Cited	MA3-925 was used in immunoprecipitation and western blot to investigate the phosphorylation of Ryanodine receptor (RyR) in rat cardiomyocytes Biochem Biophys Res Commun. 2008 Nov 7;376(1):80-5. "Ryanodine receptor phosphorylation at Serine 2030, 2808 and 2814 in rat cardiomyocytes." Author(s): Huke S, Bers DM Number of Citations: 1 (See PubMed article )

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