Part of Thermo Fisher Scientific

Rapsyn Monoclonal Antibody (1234)

Rapsyn Antibody for IF, Western blot

Synonyms:
43 kDa receptor-associated protein of the synapse, RAPsyn, Acetylcholine receptor-associated 43 kDa protein, 43 kDa postsynaptic protein
Entrez Gene ID:
(Murine) 19400
UniProt ID:
P12672
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: 1234
Species Reactivity: Murine (Ms) Rat (Rt) Chicken (Ck) Amphibian (Am) Fish (Fs)
Immunogen: Whole purified rapsyn from Torpedo californica electric organ postsynaptic membrane.
Ordering Information
Pierce Rapsyn Monoclonal Antibody (1234)
Product Number Pkg. Size Price Purchase
MA1-746 100 µg $299.00
Add Rapsyn Monoclonal Antibody (1234) to your cart.


Storage: -20º C, Avoid Freeze/Thaw Cycles
Form: 100 ug of protein G purified antibody (1 mg/ml) in PBS containing 1 mg/ml BSA and 0.05% sodium azide.


Applications Dilution *
Western Blot (WB) 0.5 ug/ml
Immunofluorescence (IF) 2 ug/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
MA1-746 detects rapysn from mouse, rat, chicken, amphibian and fish samples.

MA1-746 has been used in immunochemistry procedures using cryosections, immunocytochemistry, immunofluorescence and Western Blot procedures. By Western blot, this antibody detects a 48 kDa protein representing rapsyn from Torpedo californica electrocyte cell extracts.

The MA1-746 immunogen is whole purified rapsyn from Torpedo californica electric organ postsynaptic membrane.
General Information
Nicotinic acetylcholine receptors (AChRs) are organized into high-density postsynaptic clusters at the motor endplate that are critical for efficient synaptic transmission. The organization of AChR’s is carried out by the 43 kDa cytoplasmic protein rapsyn. Studies have indicated that mutations which impair the activity of rapsyn can lead to the formation of congenital myasthenic syndrome (CMS). CMS is characterized by poor development of the postsynaptic region.
PubMed References:
Immunocytochemistry
  Species / Dilution Summary
  Am / Not Cited
 MA1-746 was used in immunocytochemistry to investigate the regulation of AChR cluster dispersal and formation by PTPase
J Cell Biol. 1998 Jun 29;141(7):1613-24.
"A role of tyrosine phosphatase in acetylcholine receptor cluster dispersal and formation."
Author(s): Dai Z, Peng HB
Number of Citations: 5
(See PubMed article )
  Ms / Not Cited
 MA1-746 was used in immunocytochemistry to investigate the functional properties of dystroglycans in acetylcholine receptors clustering and the assembly of basement membrane
J Cell Biol. 2001 Feb 5;152(3):435-50.
"The dystroglycan complex is necessary for stabilization of acetylcholine receptor clusters at neuromuscular junctions and formation of the synaptic basement membrane."
Author(s): Jacobson C, Côté PD, Rossi SG, Rotundo RL, Carbonetto S
Number of Citations: 24
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Ch / 1:200
 MA1-746 was used in immunohistochemistry, immunoprecipitation and western blot to investigate the localization of rapsyn in comparison with those of AChR and sodium/potassium ATPase in Torpedo electrocyte
J Neurosci. 2000 Jan 15;20(2):521-8.
"The myristoylated protein rapsyn is cotargeted with the nicotinic acetylcholine receptor to the postsynaptic membrane via the exocytic pathway."
Author(s): Marchand S, Bignami F, Stetzkowski-Marden F, Cartaud J
Number of Citations: 3
(See PubMed article )
  Ms / Not Cited
 MA1-746 was used in immunohistochemistry to investigate the possible regulation of acetylcholine receptor clustering by rapsyn at interneuronal synapses
J Neurosci. 1998 Jun 1;18(11):4166-76.
"Rapsyn clusters neuronal acetylcholine receptors but is inessential for formation of an interneuronal cholinergic synapse."
Author(s): Feng G, Steinbach JH, Sanes JR
Number of Citations: 7
(See PubMed article )
  Rt / Not Cited
 MA1-746 was used in immunohistochemistry to investigate the effect of neural agrin on the expression of postsynaptic protein components
J Neurosci. 1997 Sep 1;17(17):6534-44.
"Neural agrin induces ectopic postsynaptic specializations in innervated muscle fibers."
Author(s): Meier T, Hauser DM, Chiquet M, Landmann L, Ruegg MA, Brenner HR
Number of Citations: 16
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Ch / Not Cited
 MA1-746 was used in immunohistochemistry, immunoprecipitation and western blot to investigate the localization of rapsyn in comparison with those of AChR and sodium/potassium ATPase in Torpedo electrocyte
J Neurosci. 2000 Jan 15;20(2):521-8.
"The myristoylated protein rapsyn is cotargeted with the nicotinic acetylcholine receptor to the postsynaptic membrane via the exocytic pathway."
Author(s): Marchand S, Bignami F, Stetzkowski-Marden F, Cartaud J
Number of Citations: 3
(See PubMed article )
Western Blot
  Species / Dilution Summary
  Ch / Not Cited
 MA1-746 was used in western blot to investigate the interaction between beta-dystroglycan and rapsyn
J Biol Chem. 1998 May 1;273(18):11321-6.
"Evidence for in situ and in vitro association between beta-dystroglycan and the subsynaptic 43K rapsyn protein. Consequence for acetylcholine receptor clustering at the synapse."
Author(s): Cartaud A, Coutant S, Petrucci TC, Cartaud J
Number of Citations: 17
(See PubMed article )
  Ch / 1:10,000
 MA1-746 was used in immunohistochemistry, immunoprecipitation and western blot to investigate the localization of rapsyn in comparison with those of AChR and sodium/potassium ATPase in Torpedo electrocyte
J Neurosci. 2000 Jan 15;20(2):521-8.
"The myristoylated protein rapsyn is cotargeted with the nicotinic acetylcholine receptor to the postsynaptic membrane via the exocytic pathway."
Author(s): Marchand S, Bignami F, Stetzkowski-Marden F, Cartaud J
Number of Citations: 3
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)