Rabbit anti-Human IgE (Heavy chain) Secondary Antibody

Human IgE (Heavy chain) Polyclonal Secondary Antibody for IHC (P), FACS

Details
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Secondary Antibody
Label: Unconjugated
Tested Species Reactivity: Human (Hu)
Immunogen: IgE isolated from a pool of normal human sera
Ordering Information
Pierce Rabbit anti-Human IgE (Heavy chain) Secondary Antibody
Product #
PA5-16396
Size
500 ul
Price
$140.00
Purchase
Add Rabbit anti-Human IgE (Heavy chain) Secondary Antibody to your cart.
Tested Applications Dilution *
Immunohistochemistry (Paraffin) (IHC (P)) 1:500-1:1000
Flow Cytometry (FACS) 1:10
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Purification: purified
Storage Buffer: PBS, pH 7.4, with 0.2% BSA
Preservative: 0.09% sodium azide
Storage Conditions: 4° C
Product Specific Information
PA5-16396 targets Human IgE (epsilon-Heavy Chain) in IHC (P) and FACS applications and shows reactivity with Human samples.

The PA5-16396 immunogen is igE isolated from a pool of normal human sera.
General Information
Thermo Scientific Pierce Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
Product Images
  • Human IgE (Heavy chain) Secondary Antibody (PA5-16396) in IHC
    PA5-16396_Immunohistochemistry.jpg

    Immunohistochemistry with anti-Human IgE (Heavy chain) Polyclonal Secondary Antibody (PA5-16396) (PA5-16396_Immunohistochemistry.jpg)

    Immunohistochemistry with anti-Human IgE (Heavy chain) Polyclonal Secondary Antibody (PA5-16396)

    Formalin-fixed, paraffin-embedded human tonsil stained with IgE antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of IgEecreting cells.
  • Human IgE (Heavy chain) Secondary Antibody (PA5-16396) in FACS
    PA5-16396_FACS_Ramos_20130708132045.jpg

    Flow Cytometry with anti-Human IgE (Heavy chain) Polyclonal Secondary Antibody (PA5-16396) (PA5-16396_FACS_Ramos_20130708132045.jpg)

    Flow Cytometry with anti-Human IgE (Heavy chain) Polyclonal Secondary Antibody (PA5-16396)

    Flow cytometry analysis of in Ramos cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a Rabbit anti-Human IgE secondary antibody (Product # PA5-16396) at a dilution of 1:10 for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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