* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
PBS, pH 7.4,
0.09% sodium azide
Product Specific Information
PA5-16400 targets Human IgD (delta-Heavy Chain) in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-16400 immunogen is igD isolated from a pool of normal human sera.
Thermo Scientific Pierce Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
Human IgD (Heavy chain) Secondary Antibody (PA5-16400) in WB PA5-16400_WB.jpg
Western Blot with anti-Human IgD (Heavy chain) Polyclonal Secondary Antibody (PA5-16400)
Western blot analysis of Human IgD was performed by loading 25 ug of Ramos cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a Rabbit anti-Human IgD (delta-Heavy Chain) secondary antibody (Product # PA5-16400) at a dilution of 1:200 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~50-75 kDa.
Human IgD (Heavy chain) Secondary Antibody (PA5-16400) in IHC PA5-16400_Immunohistochemistry.jpg
Immunohistochemistry with anti-Human IgD (Heavy chain) Polyclonal Secondary Antibody (PA5-16400)