* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Antigen affinity chromatography
0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Product Specific Information
RSPO4 is a member of the R-spondin family of proteins that share a common domain organization consisting of a signal peptide, cysteine-rich/furin-like domain, thrombospondin domain and a C-terminal basic region. The encoded protein may be involved in activation of Wnt/beta-catenin signaling pathways. Mutations in this gene are associated with anonychia congenital. Alternate splicing results in multiple transcript variants.
RSPO4 Antibody (PA5-25615) in WB RSPO4-_Antibody_PA5-25615_WB_1_112013.jpg
Western Blot with anti-RSPO4 Polyclonal Antibody (PA5-25615)
Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human testis tissue using a RSPO4 polyclonal antibody (Product # PA5-25615), followed by HRP-conjugated secondary antibody and DAB staining.
RSPO4 Antibody (PA5-25615) in FACS RSPO4-_Antibody_PA5-25615_FC_1_112013.jpg
Flow Cytometry with anti-RSPO4 Polyclonal Antibody (PA5-25615)
Flow cytometry analysis of Jurkat cells using a RSPO4 polyclonal antibody (Product # PA5-25615) (bottom) compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
(This product is for In Vitro experimental use only. Not for resale without express authorization.)