RSPO4 Antibody

RSPO4 Polyclonal Antibody for Western blot, IHC, FACS

Synonyms:
C20orf182, CRISTIN4, R-spondin family, member 4, R-spondin-4, hRspo4, roof plate-specific spondin-4
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu)
Immunogen: KLH conjugated synthetic peptide between 199-228 amino acids from the C-terminal region of human RSPO4
Ordering Information
Pierce RSPO4 Antibody
Product #
PA5-25615
Size
400 ul
Price
$316.00
Purchase
Add RSPO4 Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:1000
Immunohistochemistry (IHC) 1:50-1:100
Flow Cytometry (FACS) 1:10-1:50
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: Lot-specific
Purification: Antigen affinity chromatography
Storage Buffer: PBS
Preservative: 0.09% sodium azide
Storage Conditions: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Product Specific Information
General Information
RSPO4 is a member of the R-spondin family of proteins that share a common domain organization consisting of a signal peptide, cysteine-rich/furin-like domain, thrombospondin domain and a C-terminal basic region. The encoded protein may be involved in activation of Wnt/beta-catenin signaling pathways. Mutations in this gene are associated with anonychia congenital. Alternate splicing results in multiple transcript variants.
Product Images
  • RSPO4 Antibody (PA5-25615) in WB
    RSPO4-_Antibody_PA5-25615_WB_1_112013.jpg

    Western Blot with anti-RSPO4 Polyclonal Antibody (PA5-25615) (RSPO4-_Antibody_PA5-25615_WB_1_112013.jpg)

    Western Blot with anti-RSPO4 Polyclonal Antibody (PA5-25615)

    Western blot analysis in Jurkat cell lysates (15ug per lane) using a RSPO4 polyclonal antibody (Product # PA5-25615).
  • RSPO4 Antibody (PA5-25615) in IHC
    RSPO4-_Antibody_PA5-25615_IHC_1_112013.jpg

    Immunohistochemistry with anti-RSPO4 Polyclonal Antibody (PA5-25615) (RSPO4-_Antibody_PA5-25615_IHC_1_112013.jpg)

    Immunohistochemistry with anti-RSPO4 Polyclonal Antibody (PA5-25615)

    Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human testis tissue using a RSPO4 polyclonal antibody (Product # PA5-25615), followed by HRP-conjugated secondary antibody and DAB staining.
  • RSPO4 Antibody (PA5-25615) in FACS
    RSPO4-_Antibody_PA5-25615_FC_1_112013.jpg

    Flow Cytometry with anti-RSPO4 Polyclonal Antibody (PA5-25615) (RSPO4-_Antibody_PA5-25615_FC_1_112013.jpg)

    Flow Cytometry with anti-RSPO4 Polyclonal Antibody (PA5-25615)

    Flow cytometry analysis of Jurkat cells using a RSPO4 polyclonal antibody (Product # PA5-25615) (bottom) compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific