* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Antigen affinity chromatography
0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Product Specific Information
This antibody is predicted to react with bovine, chicken, hamster, mouse, rat and Xenopus based on sequence homology.
RPS13, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S15P family of ribosomal proteins. It is located in the cytoplasm. The protein has been shown to bind to the 5.8S rRNA in rat. The gene product of the E. coli ortholog (ribosomal protein S15) functions at early steps in ribosome assembly. This gene is co-transcribed with two U14 small nucleolar RNA genes, which are located in its third and fifth introns. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
RPS13 Antibody (PA5-24732) in WB RPS13_Antibody_PA5-24732_WB_1_112013.jpg
Western Blot with anti-RPS13 Polyclonal Antibody (PA5-24732)
Immunohistochemistry analysis in formalin-fixed, paraffin-embedded breast carcinoma using a RPS13 polyclonal antibody (Product # PA5-24732), followed by HRP-conjugated secondary antibody and DAB staining.
RPS13 Antibody (PA5-24732) in FACS RPS13_Antibody_PA5-24732_FC_1_112013.jpg
Flow Cytometry with anti-RPS13 Polyclonal Antibody (PA5-24732)
Flow cytometry analysis of NCI-H460 cells using a RPS13 polyclonal antibody (Product # PA5-24732) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
(This product is for In Vitro experimental use only. Not for resale without express authorization.)