RANKL / CD254 Antibody (12A668)

RANKL / CD254 Monoclonal Antibody for Western blot, IF, ICC, IHC (P), FACS

>> See 12 other antibodies for TNFSF11 (RP11-86N24.2, CD254, ODF, OPGL, OPTB2, RANKL, TRANCE, hRANKL2, sOdf, TNF-related activation-induced cytokine, TNFSF11, TRANCE)
RP11-86N24.2, CD254, ODF, OPGL, OPTB2, RANKL, TRANCE, hRANKL2, sOdf, TNF-related activation-induced cytokine, TNFSF11, TRANCE
Entrez Gene ID:
Host / Isotype: Mouse / IgG1, kappa
Class: Monoclonal
Type: Antibody
Clone: 12A668
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt)
Immunogen: A bacterially expressed fusion protein containing amino acid residues 1-317 of mouse RANKL was used as immunogen.
Ordering Information
Pierce RANKL / CD254 Antibody (12A668)
Product #
100 ug
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Tested Applications Dilution *
Western Blot (WB) 0.5-2 µg/ml
Immunofluorescence (IF) Assay-Dependent
Immunocytochemistry (ICC) Assay-Dependent
Immunohistochemistry (Paraffin) (IHC (P)) 5 µg/ml
Flow Cytometry (FACS) Assay-Dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 0.5mg/ml
Purification: Protein G
Storage Buffer: PBS with 0.05% BSA
Preservative: 0.05% sodium azide
Storage Conditions: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Specific Information
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Suggested positive control: activated T cells or RAW, antigen standard for TNFSF11 (transient overexpression lysate).
General Information
RANKL, or Trance, is a member of tumor necrosis factor family. Trance (TNF-related activation-induced cytokine receptor) or RANKL {receptor activator of NF-kB (RANK) ligand} has been implicated in interactions between T cells and DCs (1-2). RANKL binds to RANK on DCs and upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in DC survival (2). RANKL is also important in T- and B-cell maturation (3). As OPGL/ODF (Osteoprotegerin Ligand/Osteoclast differentiation factor), the same protein can both activate mature osteoclasts and mediate osteoclastogenesis (4). RANKL deficient mice show severe osteoporesis and complete absence of osteoclasts as a result of lack of osteogenesis (3).
Product Images
  • RANKL / CD254 Antibody (MA5-16156) in WB

    Western Blot with anti-RANKL / CD254 Monoclonal Antibody [12A668] (MA5-16156) (anti-CD254-RANKL--Monoclonal-Antibody-MA5-16156-WB.jpg)

    Western Blot with anti-RANKL / CD254 Monoclonal Antibody [12A668] (MA5-16156)

    Western blot analysis of a partial recombinant Trance protein probed with 2 ug/ml of a CD254/RANKL monoclonal antibody (Product # MA5-16156).
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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