RAD51 Antibody (51RAD01 (3C10))

RAD51 Monoclonal Antibody for Western blot, IF, ICC, IHC (P), IP

>> See 10 other antibodies for RAD51 (BRCA1/BRCA2-containing complex, subunit 5, DNA repair protein RAD51 homolog 1, RAD51 homolog A, RecA, E. coli, homolog of, RecA-like protein, recombination protein A)
Synonyms:
BRCA1/BRCA2-containing complex, subunit 5, DNA repair protein RAD51 homolog 1, RAD51 homolog A, RecA, E. coli, homolog of, RecA-like protein, recombination protein A
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG1, kappa
Class: Monoclonal
Type: Antibody
Clone: 51RAD01 (3C10)
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt)
Published Species Reactivity: Human (Hu), Not Applicable (N/A), Non-human primate (Nhp)
Immunogen: Recombinant Rad51 protein
Ordering Information
Pierce RAD51 Antibody (51RAD01 (3C10))
Product #
MA5-14419
Size
500 ul
Price
$335.00
Purchase
Add RAD51 Antibody (51RAD01 (3C10)) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:50-1:500
Immunofluorescence (IF) 1:10-1:100
Immunocytochemistry (ICC) 1:10-1:100
Immunohistochemistry (Paraffin) (IHC (P)) 2-4 µg/ml
Immunoprecipitation (IP) 2 µg/ml
Published Applications Dilution
Western Blot (WB) See publications below
Immunocytochemistry (ICC) See publications below
Immunohistochemistry (IHC) See publications below
Immunoprecipitation (IP) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 0.2mg/ml
Purification: Protein G
Storage Buffer: PBS, pH 7.4, with 0.2% BSA
Preservative: 0.09% sodium azide
Storage Conditions: 4° C
Product Specific Information
MA5-14419 targets RAD51 in IHC (P), IP, ICC/IF and WB applications and shows reactivity with Human, mouse, and Rat samples.

The MA5-14419 immunogen is recombinant Rad51 protein.
General Information
Rad51 (a eukaryotic homologue of Escherichia coli RecA) is similar to RecA, both biochemically and structurally: It promotes homologous pairing and strand exchange within a regular nucleoprotein filament. In yeast, the Rad51 family of related proteins also includes Rad55, Rad57 and Dmc1. In mammalian cells, seven genes in this family have been identified (HsRAD51, XRCC2, XRCC3, RAD51B/hREC2 and HsDMC1, RAD51C, and RAD 51D). Recent studies suggested that Rad51 is involved not only in homologous recombination but also in cell proliferation regulation.
Product Images
  • RAD51 Antibody (MA5-14419) in WB
    MA5-14419_WesternBlot.jpg

    Western Blot with anti-RAD51 Monoclonal Antibody [51RAD01 (3C10)] (MA5-14419) (MA5-14419_WesternBlot.jpg)

    Western Blot with anti-RAD51 Monoclonal Antibody [51RAD01 (3C10)] (MA5-14419)

    Western blot of RAD51 using RAD51 Monoclonal Antibody (MA5-14419) on HeLa Cells.
  • RAD51 Antibody (MA5-14419) in WB
    MA5-14419_WB.jpg

    Western Blot with anti-RAD51 Monoclonal Antibody [51RAD01 (3C10)] (MA5-14419) (MA5-14419_WB.jpg)

    Western Blot with anti-RAD51 Monoclonal Antibody [51RAD01 (3C10)] (MA5-14419)

    Western blot analysis of RAD51 was performed by loading 25 ug of Jurkat (lane 1), CEM (lane 2), mouse thymus (lane 3) and mouse testis (lane 4) onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a RAD51 monoclonal antibody (Product # MA5-14419) at a dilution of 1:100 (Jurkat, CEM, mouse thymus) and 1:50 (mouse testis) overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~37kDa.
  • RAD51 Antibody (MA5-14419) in IF
    MA5-14419_IF_NIH-3T3.jpg

    Immunofluorescence with anti-RAD51 Monoclonal Antibody [51RAD01 (3C10)] (MA5-14419) (MA5-14419_IF_NIH-3T3.jpg)

    Immunofluorescence with anti-RAD51 Monoclonal Antibody [51RAD01 (3C10)] (MA5-14419)

    Immunofluorescent analysis of RAD51 (green) showing staining in the cytoplasm and nucleus of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a RAD51 monoclonal antibody (Product # MA5-14419) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.
  • RAD51 Antibody (MA5-14419) in IF
    MA5-14419_IF_H1299.jpg

    Immunofluorescence with anti-RAD51 Monoclonal Antibody [51RAD01 (3C10)] (MA5-14419) (MA5-14419_IF_H1299.jpg)

    Immunofluorescence with anti-RAD51 Monoclonal Antibody [51RAD01 (3C10)] (MA5-14419)

    Immunofluorescent analysis of RAD51 (green) showing staining in the cytoplasm and nucleus of H1299 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a RAD51 monoclonal antibody (Product # MA5-14419) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Actin was stained using Alexa Fluor 554 (red) and nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.
  • RAD51 Antibody (MA5-14419) in IF
    MA5-14419_IF_Hela.jpg

    Immunofluorescence with anti-RAD51 Monoclonal Antibody [51RAD01 (3C10)] (MA5-14419) (MA5-14419_IF_Hela.jpg)

    Immunofluorescence with anti-RAD51 Monoclonal Antibody [51RAD01 (3C10)] (MA5-14419)

    Immunofluorescent analysis of RAD51 (green) showing staining in the cytoplasm and nucleus of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a RAD51 monoclonal antibody (Product # MA5-14419) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Actin was stained using Alexa Fluor 554 (red) and nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.
  • RAD51 Antibody (MA5-14419) in IHC
    MA5-14419_Immunohistochemistry.jpg

    Immunohistochemistry with anti-RAD51 Monoclonal Antibody [51RAD01 (3C10)] (MA5-14419) (MA5-14419_Immunohistochemistry.jpg)

    Immunohistochemistry with anti-RAD51 Monoclonal Antibody [51RAD01 (3C10)] (MA5-14419)

    Formalin-fixed, paraffin-embedded human testis stained with Rad 51 antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of germ cells.
  • RAD51 Antibody (MA5-14419) in IP
    MA5-14419_Immunoprecipitation.jpg

    Immunoprecipitation with anti-RAD51 Monoclonal Antibody [51RAD01 (3C10)] (MA5-14419) (MA5-14419_Immunoprecipitation.jpg)

    Immunoprecipitation with anti-RAD51 Monoclonal Antibody [51RAD01 (3C10)] (MA5-14419)

    Immunoprecipitation of RAD51 using RAD51 Monoclonal Antibody (MA5-14419) on Native Human LS174T Cells.
Publications:
Western Blot
  Species / Dilution Summary
  Hu / 2 ug/ml
MA5-14419 was used in western blot to compare the protein-protein interactions and nuclear localization signals of human and mouse BRCA2

J Biol Chem. 2001 Oct 5;276(40):37640-8.
"Analysis of murine Brca2 reveals conservation of protein-protein interactions but differences in nuclear localization signals."
Author(s): Sarkisian CJ, Master SR, Huber LJ, Ha SI, Chodosh LA
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
MA5-14419 was used in western blot to study the therapeutic efficacy of epidermal growth factor receptor signaling inhibitor erlotinib and its mechanism

Cancer Res. 2005 Apr 15;65(8):3328-35.
"Mechanisms of enhanced radiation response following epidermal growth factor receptor signaling inhibition by erlotinib (Tarceva)."
Author(s): Chinnaiyan P, Huang S, Vallabhaneni G, Armstrong E, Varambally S, Tomlins SA, Chinnaiyan AM, Harari PM
Number of Citations: 28
(See PubMed article )
  Hu / 0
MA5-14419 was used in western blot to study the effect of the glutathione levels on the interaction of radiation- and bleomycin-induced lesions

Mutagenesis. 2005 Sep;20(5):329-35.
"Interaction of radiation- and bleomycin-induced lesions and influence of glutathione level on the interaction."
Author(s): Dutta A, Chakraborty A, Saha A, Ray S, Chatterjee A
Number of Citations: 1
(See PubMed article )
  Nhp / 1:1000
MA5-14419 was used in immunocytochemistry and western blot to study the role of viral proteins and induction of the DNA repair response in the recruitment of cellular repair and recombination proteins to sites of HSV-1 replication

J Virol. 2004 May;78(9):4783-96.
"Recruitment of cellular recombination and repair proteins to sites of herpes simplex virus type 1 DNA replication is dependent on the composition of viral proteins within prereplicative sites and correlates with the induction of the DNA damage response."
Author(s): Wilkinson DE, Weller SK
Number of Citations: 71
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Hu / 1:100
MA5-14419 was used in immunocytochemistry to study the role of endopolyploid cells in the repair of DNA double strand breaks

J Cell Sci. 2003 Oct 15;116(Pt 20):4095-106.
"Endopolyploid cells produced after severe genotoxic damage have the potential to repair DNA double strand breaks."
Author(s): Ivanov A, Cragg MS, Erenpreisa J, Emzinsh D, Lukman H, Illidge TM
Number of Citations: 11
(See PubMed article )
  Hu / 1:100
MA5-14419 was used in immunocytochemistry to study the role of meiotic cohesin REC8 in chromosome segregation in gamma irradiation-induced endopolyploid tumour cells

Exp Cell Res. 2009 Sep 10;315(15):2593-603.
"The role of meiotic cohesin REC8 in chromosome segregation in gamma irradiation-induced endopolyploid tumour cells."
Author(s): Erenpreisa J, Cragg MS, Salmina K, Hausmann M, Scherthan H
Number of Citations: 3
(See PubMed article )
  N/A / 1:50
MA5-14419 was used in immunocytochemistry to study the essential role of centromere function in formation of the chromosome bouquet and inter-homolog recombination in Tetrahymena

J Cell Sci. 2012 Dec 1;125(Pt 23):5873-80.
"The Tetrahymena meiotic chromosome bouquet is organized by centromeres and promotes interhomolog recombination."
Author(s): Loidl J, Lukaszewicz A, Howard-Till RA, Koestler T
Number of Citations: 1
(See PubMed article )
  N/A / 1:50
MA5-14419 was used in immunocytochemistry to study the ability of a single Tetrahymena cohesin complex to perform both mitotic and meiotic functions

PLoS Genet. 2013 Mar;9(3):e1003418.
"A single cohesin complex performs mitotic and meiotic functions in the protist tetrahymena."
Author(s): Howard-Till RA, Lukaszewicz A, Novatchkova M, Loidl J
Number of Citations: 1
(See PubMed article )
  Nhp / 1:200
MA5-14419 was used in immunocytochemistry and western blot to study the role of viral proteins and induction of the DNA repair response in the recruitment of cellular repair and recombination proteins to sites of HSV-1 replication

J Virol. 2004 May;78(9):4783-96.
"Recruitment of cellular recombination and repair proteins to sites of herpes simplex virus type 1 DNA replication is dependent on the composition of viral proteins within prereplicative sites and correlates with the induction of the DNA damage response."
Author(s): Wilkinson DE, Weller SK
Number of Citations: 71
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Hu / 1:75
MA5-14419 was used in immunohistochemistry to study the involvement of human papillomavirus in oral atrophic lichen planus lesions

Oral Oncol. 2012 Oct;48(10):980-4.
"Human papillomavirus in oral atrophic lichen planus lesions."
Author(s): Mattila R, Rautava J, Syrjänen S
Number of Citations: 0
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Hu / Not Cited
MA5-14419 was used in immunoprecipitation to study the role of the Ref1-p53-Brca1 protein complex in seleno-L-methionine-mediated protection against DNA damage

Anticancer Res. 2006 Mar-Apr;26(2A):899-904.
"Selenium protection from DNA damage involves a Ref1/p53/Brca1 protein complex."
Author(s): Fischer JL, Lancia JK, Mathur A, Smith ML
Number of Citations: 11
(See PubMed article )
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