* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Antigen affinity chromatography
PBS, pH 7,
1% BSA, 20% glycerol
-20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA5-28321 targets Protein C in IF, IHC (P), and WB applications and shows reactivity with Human samples.
The PA5-28321 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 236 of Human Protein C.
The PROC gene encodes protein C (EC 126.96.36.199), a vitamin K-dependent plasma glycoprotein that is a key component of the anticoagulant system. Protein C is cleaved to its activated form, 'activated protein C' (APC) on endothelial cells by the thrombin-thrombomodulin complex (MIM 176930; MIM 188040) and then acts as a serine protease to degrade the activated forms of coagulation factors V (F5; MIM 612309) and VIII (F8; see MIM 306700). Protein S (PROS1; MIM 176880), also a vitamin K-dependent plasma protein, functions as a cofactor to activated protein C.
Protein C Antibody (PA5-28321) in WB PA5-28321_WB.jpg
Western Blot with anti-Protein C Polyclonal Antibody (PA5-28321)
Western blot analysis of Protein C using 30µg of A) HepG2 and B) HCT116 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Protein C polyclonal antibody (Product # PA5-28321) at a dilution of 1:1000.
Protein C Antibody (PA5-28321) in IF PA5-28321_IF.jpg
Immunofluorescence with anti-Protein C Polyclonal Antibody (PA5-28321)