Presenilin 2 Antibody (APS 26)

Presenilin 2 Monoclonal Antibody for Western blot, IF, ICC, IHC, IP, ELISA

>> See 2 other antibodies for PSEN2 (PS2, AD4, Alzheimer disease 4, PS2, PSEN 2, STM2)
Synonyms:
PS2, AD4, Alzheimer disease 4, PS2, PSEN 2, STM2
Entrez Gene ID:
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: APS 26
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt), Non-human primate (Nhp)
Published Species Reactivity: Human (Hu)
Immunogen: Synthetic peptide corresponding to residues C L(317) P Y D P E M E E D S Y D S F G E P(334) of human PS2.
Ordering Information
Pierce Presenilin 2 Antibody (APS 26)
Product #
MA1-754
Size
200 µg
Price
$316.00
Purchase
Add Presenilin 2 Antibody (APS 26) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:100-1:1000
Immunofluorescence (IF) 0.75 ug/ml
Immunocytochemistry (ICC) Assay dependent
Immunohistochemistry (IHC) 1:20
Immunoprecipitation (IP) Assay Dependent
ELISA (ELISA) 4.5 ug/ml
Published Applications Dilution
Western Blot (WB) See publications below
Immunocytochemistry (ICC) See publications below
Immunoprecipitation (IP) See publications below
ELISA (ELISA) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Protein G
Storage Buffer: PBS with 1mg/ml BSA
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
MA1-754 detects presenilin 2 protein (PS2) from rat, mouse, human, and nonhuman primate samples. No cross-reactivity is seen with presenilin 1.

MA1-754 has successfully been used in immuno-fluorescence, immunocytochemistry, Western blot and ELISA procedures. By Western blot, this antibody detects an ~20 kDa protein representing PS2 CT (C-terminal fragment) and the ~45 kDa full-length PS2 from transfected COS-7 cells. In 4% paraformaldehyde fixed RAW cells, MA1-754 showed specific Golgi and ER labeling.

The MA1-754 immunogen is a synthetic peptide corresponding to residues C L(317) P Y D P E M E E D S Y D S F G E P(334) of human PS2.
General Information
Familial Alzheimer’s disease is often characterized by an early (<60 years of age) and rapid deterioration of the central nervous system. The symptoms are caused by the abnormal buildup of senile plaques composed of the 42 residue amyloid-beta peptide. This peptide is the result of the amyloid precursor protein being cleaved by the presenilin-gamma-secretase complex. Recent studies have linked mutations in presenilin 1 (PS1) and presenilin 2 (PS2) to a rapid increase in plaque accumulation.

PS1 and PS2 are integral membrane proteins that contain 6-8 transmembrane domains and are predominantly localized within the endoplasmic reticulum and Golgi of neurons within the brain. Within the transmembrane regions of PS1 and PS2 there is over 60% homology, with the largest divergence found at the N-terminus and large loop region.
Product Images
  • Presenilin 2 Antibody (MA1-754) in WB
    MA1-754_WB.jpg

    Western Blot with anti-Presenilin 2 Monoclonal Antibody [APS 26] (MA1-754) (MA1-754_WB.jpg)

    Western Blot with anti-Presenilin 2 Monoclonal Antibody [APS 26] (MA1-754)

    Western blot analysis of Presenilin 2 was performed by loading 25 ug of human brain (lane 1) and L929 (lane 2) onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a Presenilin 2 monoclonal antibody (Product # MA1-754) at a dilution of 1:200 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~20kDa.
  • Presenilin 2 Antibody (MA1-754) in IF
    Presenilin-2_MA1-754_Immunofluorescence_A2058-Cells.jpg

    Immunofluorescence with anti-Presenilin 2 Monoclonal Antibody [APS 26] (MA1-754) (Presenilin-2_MA1-754_Immunofluorescence_A2058-Cells.jpg)

    Immunofluorescence with anti-Presenilin 2 Monoclonal Antibody [APS 26] (MA1-754)

    Immunofluorescent analysis of Presenilin 2 using Anti-Presenilin 2 Monoclonal Antibody (APS 26) (Product# MA1-754) shows staining in A2058 Cells. Presenilin 2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Presenilin 2 (Product# MA1-754) at a dilution of 1:20 over night at 4 ◦C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35503, Goat Anti-Mouse). Images were taken at 60X magnification.
  • Presenilin 2 Antibody (MA1-754) in IF
    Presenilin-2_MA1-754_Immunofluorescence_Hela-Cells.jpg

    Immunofluorescence with anti-Presenilin 2 Monoclonal Antibody [APS 26] (MA1-754) (Presenilin-2_MA1-754_Immunofluorescence_Hela-Cells.jpg)

    Immunofluorescence with anti-Presenilin 2 Monoclonal Antibody [APS 26] (MA1-754)

    Immunofluorescent analysis of Presenilin 2 using Anti-Presenilin 2 Monoclonal Antibody (APS 26) (Product# MA1-754) shows staining in Hela Cells. Presenilin 2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Presenilin 2 (Product# MA1-754) at a dilution of 1:20 over night at 4 ◦C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35503, Goat Anti-Mouse). Images were taken at 60X magnification.
  • Presenilin 2 Antibody (MA1-754) in ICC
    Psen2-Antibody-MA1-754-Immunocytochemistry-ICC.jpg

    Immunocytochemistry with anti-Presenilin 2 Monoclonal Antibody [APS 26] (MA1-754) (Psen2-Antibody-MA1-754-Immunocytochemistry-ICC.jpg)

    Immunocytochemistry with anti-Presenilin 2 Monoclonal Antibody [APS 26] (MA1-754)

    Immunocytochemical staining of PS2 in mouse fibroblasts using MA1-754.
  • Presenilin 2 Antibody (MA1-754) in IHC
    MA1-754_IHC_Kidney_tissue.jpg

    Immunohistochemistry with anti-Presenilin 2 Monoclonal Antibody [APS 26] (MA1-754) (MA1-754_IHC_Kidney_tissue.jpg)

    Immunohistochemistry with anti-Presenilin 2 Monoclonal Antibody [APS 26] (MA1-754)

    Immunohistochemistry was performed on normal biopsies of deparaffinized Human kidney tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Presenilin 2 (MA1-754) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Publications:
Western Blot
  Species / Dilution Summary
  Hu / 4 ug/ml
MA1-754 was used in ELISA, immunocytochemistry and western blot to study the presenilin 1 and presenilin 2 expression and processing by monoclonal antibodies

J Neurosci Res. 1999 May 15;56(4):405-19.
"Analysis of presenilin 1 and presenilin 2 expression and processing by newly developed monoclonal antibodies."
Author(s): Diehlmann A, Ida N, Weggen S, Grünberg J, Haass C, Masters CL, Bayer TA, Beyreuther K
Number of Citations: 3
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Hu / 0.05 ug/ul
MA1-754 was used in ELISA, immunocytochemistry and western blot to study the presenilin 1 and presenilin 2 expression and processing by monoclonal antibodies

J Neurosci Res. 1999 May 15;56(4):405-19.
"Analysis of presenilin 1 and presenilin 2 expression and processing by newly developed monoclonal antibodies."
Author(s): Diehlmann A, Ida N, Weggen S, Grünberg J, Haass C, Masters CL, Bayer TA, Beyreuther K
Number of Citations: 3
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Hu / Not Cited
MA1-754 was used in immunoprecipitation to investigate the impacts of caspase activation on heterodimeric presenilin 1 complex and the interaction between PS1 and beta-catenin

J Biol Chem. 1998 Dec 18;273(51):33909-14.
"Abrogation of the presenilin 1/beta-catenin interaction and preservation of the heterodimeric presenilin 1 complex following caspase activation."
Author(s): Tesco G, Kim TW, Diehlmann A, Beyreuther K, Tanzi RE
Number of Citations: 1
(See PubMed article )
ELISA
  Species / Dilution Summary
  Hu / 3 ug/ml
MA1-754 was used in ELISA, immunocytochemistry and western blot to study the presenilin 1 and presenilin 2 expression and processing by monoclonal antibodies

J Neurosci Res. 1999 May 15;56(4):405-19.
"Analysis of presenilin 1 and presenilin 2 expression and processing by newly developed monoclonal antibodies."
Author(s): Diehlmann A, Ida N, Weggen S, Grünberg J, Haass C, Masters CL, Bayer TA, Beyreuther K
Number of Citations: 3
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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