Presenilin 1 Antibody (APS 11)

Presenilin 1 Monoclonal Antibody for Western blot, IF, ICC, IHC (P), IHC (PFA), IP, ELISA

>> See 6 other antibodies for PSEN1 (PS1, AD3, Alzheimer Disease 3, FAD, Homo Sapiens Clone CC44 Senilin 1, PSEN1, PSNL1, S182 Protein, Senilin 1)
Synonyms:
PS1, AD3, Alzheimer Disease 3, FAD, Homo Sapiens Clone CC44 Senilin 1, PSEN1, PSNL1, S182 Protein, Senilin 1
Entrez Gene ID:
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: APS 11
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt), Non-human primate (Nhp)
Published Species Reactivity: Human (Hu)
Immunogen: Synthetic peptide corresponding to residues C H(21) L S N T V R S Q N D N R E(34) of human PS1.
Ordering Information
Pierce Presenilin 1 Antibody (APS 11)
Product #
MA1-751
Size
200 µg
Price
$360.00
Purchase
Add Presenilin 1 Antibody (APS 11) to your cart.
Tested Applications Dilution *
Western Blot (WB) 12 ug/ml
Immunofluorescence (IF) 0.75 ug/ml
Immunocytochemistry (ICC) Assay dependent
Immunohistochemistry (Paraffin) (IHC (P)) 0.75 ug/ml
Immunohistochemistry (PFA fixed) (IHC (PFA)) Assay Dependent
Immunoprecipitation (IP) Assay Dependent
ELISA (ELISA) 4.5 ug/ml
Published Applications Dilution
Western Blot (WB) See publications below
Immunocytochemistry (ICC) See publications below
ELISA (ELISA) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Protein G
Storage Buffer: PBS with 1mg/ml BSA
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
MA1-751 detects presenilin 1 protein (PS1) from mouse, rat, human, and nonhuman primate samples. No cross-reactivity is seen with presenilin 2.

MA1-751 has successfully been used immunofluorescence, immunocytochemistry, immunohistochemistry, Western blot , and ELISA procedures. By Western blot, this antibody detects an ~28 kDa protein representing PS1 N-terminus cleavage product from ST15 cell lysate transfected with human PS1. In formalin-fixed, paraffin embedded sections of human brain, this antibody showed strong staining of both the plaque core and dystrophic neurites.

The MA1-751 immunogen is a synthetic peptide corresponding to residues C H(21) L S N T V R S Q N D N R E(34) of human PS1.

General Information
Familial Alzheimer’s disease is often characterized by an early (<60 years of age) and rapid deterioration of the central nervous system. The symptoms are caused by the abnormal buildup of senile plaques composed of the 42 residue amyloid-beta peptide. This peptide is the result of the amyloid precursor protein being cleaved by the presenilin-gamma-secretase complex. Recent studies have linked mutations in presenilin 1 (PS1) and presenilin 2 (PS2) to a rapid increase in plaque accumulation.

PS1 and PS2 are integral membrane proteins that contain 6-8 transmembrane domains and are predominantly localized within the endoplasmic reticulum and Golgi of neurons within the brain. Within the transmembrane regions of PS1 and PS2 there is over 60% homology, with the largest divergence found at the N-terminus and large loop region.
Product Images
  • Presenilin 1 Antibody (MA1-751) in ICC
    PSEN1_Antibody_MA1-751_Immunocytochemistry(ICC).jpg

    Immunocytochemistry with anti-Presenilin 1 Monoclonal Antibody [APS 11] (MA1-751) (PSEN1_Antibody_MA1-751_Immunocytochemistry(ICC).jpg)

    Immunocytochemistry with anti-Presenilin 1 Monoclonal Antibody [APS 11] (MA1-751)

    Immunocytochemical staining of PS1 in mouse fibroblasts using MA1-751.
  • Presenilin 1 Antibody (MA1-751) in IHC
    MA1-751_IHC_Brain_Tissue.jpg

    Immunohistochemistry with anti-Presenilin 1 Monoclonal Antibody [APS 11] (MA1-751) (MA1-751_IHC_Brain_Tissue.jpg)

    Immunohistochemistry with anti-Presenilin 1 Monoclonal Antibody [APS 11] (MA1-751)

    Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Presenilin 1 (MA1-751) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  • Presenilin 1 Antibody (MA1-751) in IHC
    MA1-751_IHC_Liver_Tissue.jpg

    Immunohistochemistry with anti-Presenilin 1 Monoclonal Antibody [APS 11] (MA1-751) (MA1-751_IHC_Liver_Tissue.jpg)

    Immunohistochemistry with anti-Presenilin 1 Monoclonal Antibody [APS 11] (MA1-751)

    Immunohistochemistry was performed on normal biopsies of deparaffinized Human liver tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Presenilin 1 (MA1-751) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Publications:
Western Blot
  Species / Dilution Summary
  Hu / 4 ug/ml
MA1-751 was used in ELISA, immunocytochemistry and western blot to study the presenilin 1 and presenilin 2 expression and processing by monoclonal antibodies

J Neurosci Res. 1999 May 15;56(4):405-19.
"Analysis of presenilin 1 and presenilin 2 expression and processing by newly developed monoclonal antibodies."
Author(s): Diehlmann A, Ida N, Weggen S, Grünberg J, Haass C, Masters CL, Bayer TA, Beyreuther K
Number of Citations: 3
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Hu / 0.05 ug/ul
MA1-751 was used in ELISA, immunocytochemistry and western blot to study the presenilin 1 and presenilin 2 expression and processing by monoclonal antibodies

J Neurosci Res. 1999 May 15;56(4):405-19.
"Analysis of presenilin 1 and presenilin 2 expression and processing by newly developed monoclonal antibodies."
Author(s): Diehlmann A, Ida N, Weggen S, Grünberg J, Haass C, Masters CL, Bayer TA, Beyreuther K
Number of Citations: 3
(See PubMed article )
ELISA
  Species / Dilution Summary
  Hu / 3 ug/ml
MA1-751 was used in ELISA, immunocytochemistry and western blot to study the presenilin 1 and presenilin 2 expression and processing by monoclonal antibodies

J Neurosci Res. 1999 May 15;56(4):405-19.
"Analysis of presenilin 1 and presenilin 2 expression and processing by newly developed monoclonal antibodies."
Author(s): Diehlmann A, Ida N, Weggen S, Grünberg J, Haass C, Masters CL, Bayer TA, Beyreuther K
Number of Citations: 3
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific