Part of Thermo Fisher Scientific

Pregnancy-assoc. plasma protein A Monoclonal Antibody

Pregnancy-assoc. plasma protein A Antibody for ELISA, IHC, Western blot

Synonyms:
PAPP-A, Insulin-like growth factor-dependent IGF-binding protein 4 protease, IGF-dependent IGFBP-4 protease, IGFBP-4 ase
Entrez Gene ID:
(Human) 5069
UniProt ID:
Q13219
Details
Host / Isotype: Mouse / IgG2b
Class: Monoclonal
Type: Antibody
Species Reactivity: Human (Hu)
Immunogen: Purified human PAPP-A.
Ordering Information
Pierce Pregnancy-assoc. plasma protein A Monoclonal Antibody
Product Number Pkg. Size Price Purchase
BTE 004-09-02 200 µg $362.00
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Storage: 4º C
Form: 200 µg of Protein A/G purified antibody (1 mg/ml) in 0.01 M phosphate buffer, with 0.5 M NaCl and 15 mM sodium azide; pH 7.4.


Applications Dilution *
Immunohistochemistry (IHC) Assay dependent
ELISA (ELISA) 1:8,000
Western Blot (WB) Assay dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
BTE 004-09-02 detects Pregnancy-associated plasma protein A (PAPP-A) from human samples. Heat and SDS denatured PAPP-A is also recognized, but not
acid treated PAPP-A.

 BTE 004-09-02 has been successfully used in ELISA, Western blotting and Immunohistochemistry procedures. In sandwich ELISA the optimal combination is BTE 004-09-02 used as coating antibody and biotinylated ABS 006-24-02 used as detection antibody. BTE 004-09-02 can also be used as detection antibody in sandwich ELISA in combination with a polyclonal antibody towards PAPP-A in coat. BTE 004-09-02 detect both reduced and unreduced PAPP-A in Western Blotting, though reaction to unreduced is less than that of ABS 006-01-02 and ABS 006-24-02.

The BTE 004-09-02 immunogen is purified human PAPP-A.

The epitope of BTE 004-09-02 is different from ABS 006-01-02 and ABS 006-24-02. The epitope is located on the PAPP-A subunit of the heterotetramer.
General Information
Pregnancy-associated plasma protein-A (PAPP-A, Insulin-like growth factor binding protein-4 protease, PAPP-A-proMBP complex) is a large heterottetrameric glycoprotein of approximately 500 kDa, which was first discovered in serum from pregnant women. The hetreotetramer consists of two PAPP-A subunits and two proMBP subunits. Low maternal serum levels of PAPP-A in first trimester biochemical screening is used as a marker of Down’s syndrome (trisomy 21).
(This product is for In Vitro experimental use only. Not for resale without express authorization.)