Phospholamban Antibody (2D12)

Phospholamban Monoclonal Antibody for Western blot, IF, ICC, IHC (P), IP

>> See 3 other antibodies for PLN (PLB, Cardiac phospholamban. PLN)
Synonyms:
PLB, Cardiac phospholamban. PLN
Details
Host / Isotype: Mouse / IgG2a
Class: Monoclonal
Type: Antibody
Clone: 2D12
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt), Canine (Ca), Chicken (Ck), Guinea Pig (GP), Ovine (Ov), Porcine (Po), Rabbit (Rb)
Published Species Reactivity: Canine (Ca), Guinea Pig (GP), Human (Hu), Mouse (Ms), Porcine (Po), Rabbit (Rb), Rodent (Ro), Rat (Rt)
Immunogen: Synthetic peptide corresponding to residues D(2) K V Q Y L T R S A I R R A S T I E M P Q Q A R(25) of canine PLB.
Ordering Information
Pierce Phospholamban Antibody (2D12)
Product #
MA3-922
Size
100 µl
Price
$360.00
Purchase
Add Phospholamban Antibody (2D12) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:500-1:5000
Immunofluorescence (IF) 1:10-1:100
Immunocytochemistry (ICC) 1:10-1:100
Immunohistochemistry (Paraffin) (IHC (P)) 1:200
Immunoprecipitation (IP) Assay dependent
Published Applications Dilution
Western Blot (WB) See publications below
Immunocytochemistry (ICC) See publications below
Immunohistochemistry (IHC) See publications below
Blocking Assay (BLOCK) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Storage Buffer: ascites diluted in PBS
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
MA3-922 detects phospholamban (PLB) in rabbit, human, pig, sheep, chicken, canine, guinea porcine, rat, and mouse.

MA3-922 has been successfully used in Western blot, immunofluorescence, immunohistochemistry and immunoprecipitation procedures. By Western blot, this antibody detects a 25 kDa protein under non-reducing conditions representing the pentameric form of PLB and a 5 kDa protein under reducing conditions representing the monomeric form of PLB in canine cardiac extracts. Immunofluorescence staining of PLB in rat papillary muscle tissue with MA3-922 results in diffuse staining of the network sarcoplasmic reticulum (SR). MA3-922 can also be used to increase the calcium uptake in ventricular SR vesicles analogous to the phosphorylation of PLB following adrenergic stimulation. This antibody is recommended for immunofluorescent staining of tissues.

The MA3-922 immunogen is a synthetic peptide corresponding to residues D(2) K V Q Y L T R S A I R R A S T I E M P Q Q A R(25) of canine PLB. This antibody recognizes an epitope between amino acid residues 9-17 of canine PLB.
General Information
Phospholamban (PLB) is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. The cytoplasmic domain (residues 1-25) contains the phosphorylation sites and is highly basic and readily cleaved by proteases; whereas the transmembrane domain (residues 25-52) is mostly hydrophobic, protease resistant and stabilizes the pentamer. Following adrenergic stimulation of cardiac muscle, PLB is phosphorylated at Ser16 and at Thr17 which is correlated with stimulation of calcium transport activity across the SR membrane and relaxation of cardiac fibers
Product Images
  • Phospholamban Antibody (MA3-922) in WB
    MA3-922_WB.jpg

    Western Blot with anti-Phospholamban Monoclonal Antibody [2D12] (MA3-922) (MA3-922_WB.jpg)

    Western Blot with anti-Phospholamban Monoclonal Antibody [2D12] (MA3-922)

    Western blot analysis of Phospholamban was performed by loading 25 ug of human heart (lane 1) and C2C12 (lane 2) onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a Phospholamban monoclonal antibody (Product # MA3-922) at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~25kDa.
  • Phospholamban Antibody (MA3-922) in IF
    MA3-922_IF_human_heart_tissue.jpg

    Immunofluorescence with anti-Phospholamban Monoclonal Antibody [2D12] (MA3-922) (MA3-922_IF_human_heart_tissue.jpg)

    Immunofluorescence with anti-Phospholamban Monoclonal Antibody [2D12] (MA3-922)

    Immunofluorescent analysis of Phospholamban (green) showing staining in the cytoplasm of human heart tissue (right) compared to a negative control without primary antibody (left). Formalin-fixed tissue was permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Tissue was probed with a Phospholamban monoclonal antibody (Product # MA3-922) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Tissue was washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.
  • Phospholamban Antibody (MA3-922) in IF
    MA3-922_IF_mouse_heart_tissue.jpg

    Immunofluorescence with anti-Phospholamban Monoclonal Antibody [2D12] (MA3-922) (MA3-922_IF_mouse_heart_tissue.jpg)

    Immunofluorescence with anti-Phospholamban Monoclonal Antibody [2D12] (MA3-922)

    Immunofluorescent analysis of Phospholamban (green) showing staining in the cytoplasm of mouse heart tissue (right) compared to a negative control without primary antibody (left). Formalin-fixed tissue was permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Tissue was probed with a Phospholamban monoclonal antibody (Product # MA3-922) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Tissue was washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.
  • Phospholamban Antibody (MA3-922) in IF
    MA3-922_IF_C2C12.jpg

    Immunofluorescence with anti-Phospholamban Monoclonal Antibody [2D12] (MA3-922) (MA3-922_IF_C2C12.jpg)

    Immunofluorescence with anti-Phospholamban Monoclonal Antibody [2D12] (MA3-922)

    Immunofluorescent analysis of Phospholamban (green) showing staining in the cytoplasm of C2C12 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Phospholamban monoclonal antibody (Product # MA3-922) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Actin was stained using Alexa Fluor 554 (red) and nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.
  • Phospholamban Antibody (MA3-922) in IHC
    MA3-922_Immunohistochemistry_Heart tissue.jpg

    Immunohistochemistry with anti-Phospholamban Monoclonal Antibody [2D12] (MA3-922) (MA3-922_Immunohistochemistry_Heart tissue.jpg)

    Immunohistochemistry with anti-Phospholamban Monoclonal Antibody [2D12] (MA3-922)

    Immunohistochemistry was performed on normal deparaffinized Human heart tissue tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Phospholamban (MA3-922) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  • Phospholamban Antibody (MA3-922) in IHC
    MA3-922_Immunohistochemistry_Skeletal muscle.jpg

    Immunohistochemistry with anti-Phospholamban Monoclonal Antibody [2D12] (MA3-922) (MA3-922_Immunohistochemistry_Skeletal muscle.jpg)

    Immunohistochemistry with anti-Phospholamban Monoclonal Antibody [2D12] (MA3-922)

    Immunohistochemistry was performed on normal deparaffinized Human skeletal muscle tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Phospholamban (MA3-922) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Publications:
Western Blot
  Species / Dilution Summary
  Ca / 1:500
MA3-922 was used in western blot to study the expression of the phospholamban protein in stimulated muscles and the corresponding functions

J Biol Chem. 1992 Dec 25;267(36):26056-61.
"Phospholamban expressed in slow-twitch and chronically stimulated fast-twitch muscles minimally affects calcium affinity of sarcoplasmic reticulum Ca(2+)-ATPase."
Author(s): Briggs FN, Lee KF, Wechsler AW, Jones LR
Number of Citations: 9
(See PubMed article )
  Ca / Not Cited
MA3-922 was used in western blot to investigate how coordinated expression of SERCA2a and phospholamban is achieved during skeletal muscle phenotype switching

J Biol Chem. 1995 May 12;270(19):11619-22.
"Transcriptional regulation of phospholamban gene and translational regulation of SERCA2 gene produces coordinate expression of these two sarcoplasmic reticulum proteins during skeletal muscle phenotype switching."
Author(s): Hu P, Yin C, Zhang KM, Wright LD, Nixon TE, Wechsler AS, Spratt JA, Briggs FN
Number of Citations: 3
(See PubMed article )
  Ca / Not Cited
MA3-922 was used in western blot and blocking/activating experiment to investigate the mechanism for the effect of phospholamban on calcium transport ATPase

J Biol Chem. 1993 Jun 5;268(16):11486-8.
"Residues 2-25 of phospholamban are insufficient to inhibit Ca2+ transport ATPase of cardiac sarcoplasmic reticulum."
Author(s): Jones LR, Field LJ
Number of Citations: 4
(See PubMed article )
  Ca / Not Cited
MA3-922 was used in blocking/activating experiment and western blot to investigate the mechanism for the effect of phospholamban on cardiac calcium ATPase

J Biol Chem. 1996 Jun 21;271(25):14964-70.
"Purified, reconstituted cardiac Ca2+-ATPase is regulated by phospholamban but not by direct phosphorylation with Ca2+/calmodulin-dependent protein kinase."
Author(s): Reddy LG, Jones LR, Pace RC, Stokes DL
Number of Citations: 9
(See PubMed article )
  Ca / Not Cited
MA3-922 was used in western blot to demonstrate that the mechanisms of altered excitation-contraction coupling in canine are similar to those reported in human heart failure

Circ Res. 1999 Mar 19;84(5):562-70.
"Mechanisms of altered excitation-contraction coupling in canine tachycardia-induced heart failure, I: experimental studies."
Author(s): O'Rourke B, Kass DA, Tomaselli GF, Kääb S, Tunin R, Marbán E
Number of Citations: 55
(See PubMed article )
  Ca / 1:5,000
MA3-922 was used in western blot to investigate the role of RyRs, SERCA2a, and phospholamban (PLB) in canine and human heart failure

Circ Res. 2002 Nov 29;91(11):1015-22.
"Abnormal Ca2+ release, but normal ryanodine receptors, in canine and human heart failure."
Author(s): Jiang MT, Lokuta AJ, Farrell EF, Wolff MR, Haworth RA, Valdivia HH
Number of Citations: 34
(See PubMed article )
  Ca / 1:5,000
MA3-922 was used in western blot to examine the regional changes in protein expression during heart failure

Circulation. 2003 Aug 26;108(8):929-32.
"Regional alterations in protein expression in the dyssynchronous failing heart."
Author(s): Spragg DD, Leclercq C, Loghmani M, Faris OP, Tunin RS, DiSilvestre D, McVeigh ER, Tomaselli GF, Kass DA
Number of Citations: 1
(See PubMed article )
  Ca / 1:2000
MA3-922 was used in western blot to study the effect of earlier tachycardia on infarct size

J Mol Cell Cardiol. 2003 Dec;35(12):1429-37.
"Effect of tachycardia on myocardial sarcoplasmic reticulum and Ca2+ dynamics: a mechanism for preconditioning?"
Author(s): Domenech RJ, Sánchez G, Donoso P, Parra V, Macho P
Number of Citations: 1
(See PubMed article )
  Hu / 1:500
MA3-922 was used in western blot to investigate the expression of SERCA2 ATPase, calsequestrin and phospholamban in nonfailing and failing human left ventricular myocardium

Circulation. 1994 Aug;90(2):653-7.
"Ca(2+)-transporting ATPase, phospholamban, and calsequestrin levels in nonfailing and failing human myocardium."
Author(s): Movsesian MA, Karimi M, Green K, Jones LR
Number of Citations: 11
(See PubMed article )
  Hu / Not Cited
MA3-922 was used in western blot to study the role of mAKAP and ryanodine receptor in cardiomyocyte nuclear envelope signalling.

J Cell Sci. 2001 Sep;114(Pt 17):3167-76.
"mAKAP and the ryanodine receptor are part of a multi-component signaling complex on the cardiomyocyte nuclear envelope."
Author(s): Kapiloff MS, Jackson N, Airhart N
Number of Citations: 14
(See PubMed article )
  Hu / Not Cited
MA3-922 was used in western blot to demonstrate the role of type 1 phosphatase in heart function.

Mol Cell Biol. 2002 Jun;22(12):4124-35.
"Type 1 phosphatase, a negative regulator of cardiac function."
Author(s): Carr AN, Schmidt AG, Suzuki Y, del Monte F, Sato Y, Lanner C, Breeden K, Jing SL, Allen PB, Greengard P, Yatani A, Hoit BD, Grupp IL, Hajjar RJ, DePaoli-Roach AA, Kranias EG
Number of Citations: 26
(See PubMed article )
  Hu / 1:5,000
MA3-922 was used in western blot to investigate the role of RyRs, SERCA2a, and phospholamban (PLB) in canine and human heart failure

Circ Res. 2002 Nov 29;91(11):1015-22.
"Abnormal Ca2+ release, but normal ryanodine receptors, in canine and human heart failure."
Author(s): Jiang MT, Lokuta AJ, Farrell EF, Wolff MR, Haworth RA, Valdivia HH
Number of Citations: 34
(See PubMed article )
  Hu / 1:5,000
MA3-922 was used in western blot to demonstrate that CaMKII phosphorylation could regulate the cardiac ryanodine receptor and increase RyR2 calcium sensitivity

Circ Res. 2004 Apr 2;94(6):e61-70.
"Ca2+/calmodulin-dependent protein kinase II phosphorylation regulates the cardiac ryanodine receptor."
Author(s): Wehrens XH, Lehnart SE, Reiken SR, Marks AR
Number of Citations: 1
(See PubMed article )
  Hu / 1:500
MA3-922 was used in western blot to study the functions of hESC-CMs in intracellular calcium handling and the importance of SR in the contraction process

Stem Cells. 2006 Feb;24(2):236-45.
"Functional properties of human embryonic stem cell-derived cardiomyocytes: intracellular Ca2+ handling and the role of sarcoplasmic reticulum in the contraction."
Author(s): Dolnikov K, Shilkrut M, Zeevi-Levin N, Gerecht-Nir S, Amit M, Danon A, Itskovitz-Eldor J, Binah O
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
MA3-922 was used in western blot to investigate the molecular basis of the aberrant calcium handling during heart failure.

Am J Physiol Heart Circ Physiol. 2007 Mar;292(3):H1607-18.
"Cellular and molecular determinants of altered Ca2+ handling in the failing rabbit heart: primary defects in SR Ca2+ uptake and release mechanisms."
Author(s): Armoundas AA, Rose J, Aggarwal R, Stuyvers BD, O'rourke B, Kass DA, Marbán E, Shorofsky SR, Tomaselli GF, William Balke C
Number of Citations: 1
(See PubMed article )
  Hu / 1:2000
MA3-922 was used in western blot to evaluate a proteomic method for heart membrane proteins

Proteomics. 2008 Sep;8(18):3895-905.
"Nonionic detergent phase extraction for the proteomic analysis of heart membrane proteins using label-free LC-MS."
Author(s): Donoghue PM, Hughes C, Vissers JP, Langridge JI, Dunn MJ
Number of Citations: 1
(See PubMed article )
  Hu / 1:2500
MA3-922 was used in western blot to investigate the different phenotypes between idiopathic dilated cardiomyopathy and ischemic heart disease

J Muscle Res Cell Motil. 2010 Dec;31(4):289-301.
"More severe cellular phenotype in human idiopathic dilated cardiomyopathy compared to ischemic heart disease."
Author(s): Hamdani N, Borbély A, Veenstra SP, Kooij V, Vrydag W, Zaremba R, Dos Remedios C, Niessen HW, Michel MC, Paulus WJ, Stienen GJ, van der Velden J
Number of Citations: 3
(See PubMed article )
  Ms / Not Cited
MA3-922 was used in western blot and blocking/activating experiment to investigate the mechanism for the effect of phospholamban on calcium transport ATPase

J Biol Chem. 1993 Jun 5;268(16):11486-8.
"Residues 2-25 of phospholamban are insufficient to inhibit Ca2+ transport ATPase of cardiac sarcoplasmic reticulum."
Author(s): Jones LR, Field LJ
Number of Citations: 4
(See PubMed article )
  Ms / 1:1200
MA3-922 was used in western blot to study the regulatory role of phosphorylation status on calcium handling in mouse fast- and slow-twitch skeletal muscle fibers

Am J Physiol. 1997 Dec;273(6 Pt 1):C1915-24.
"Regulation of Ca2+ handling by phosphorylation status in mouse fast- and slow-twitch skeletal muscle fibers."
Author(s): Liu Y, Kranias EG, Schneider MF
Number of Citations: 6
(See PubMed article )
  Ms / Not Cited
MA3-922 was used in western blot to investigate the mechanism underlying depressed cardiac function caused by PKC beta 2-mediated phosphorylation

J Clin Invest. 1998 Jul 1;102(1):72-8.
"In vivo phosphorylation of cardiac troponin I by protein kinase Cbeta2 decreases cardiomyocyte calcium responsiveness and contractility in transgenic mouse hearts."
Author(s): Takeishi Y, Chu G, Kirkpatrick DM, Li Z, Wakasaki H, Kranias EG, King GL, Walsh RA
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
MA3-922 was used in western blot to investigate the inhibitory activity of N27A mutant form by using transgenic mice with cardiac-specific overexpression of mutant phospholamban.

J Biol Chem. 2000 Apr 7;275(14):10538-44.
"Cardiac-specific overexpression of a superinhibitory pentameric phospholamban mutant enhances inhibition of cardiac function in vivo."
Author(s): Zhai J, Schmidt AG, Hoit BD, Kimura Y, MacLennan DH, Kranias EG
Number of Citations: 11
(See PubMed article )
  Ms / Not Cited
MA3-922 was used in western blot to investigate the role of phospholamban (PLB) in the development of cardiomyopathy and early mortality

Circulation. 2001 Feb 13;103(6):889-96.
"Interactions between phospholamban and beta-adrenergic drive may lead to cardiomyopathy and early mortality."
Author(s): Dash R, Kadambi V, Schmidt AG, Tepe NM, Biniakiewicz D, Gerst MJ, Canning AM, Abraham WT, Hoit BD, Liggett SB, Lorenz JN, Dorn GW 2nd, Kranias EG
Number of Citations: 7
(See PubMed article )
  Ms / Not Cited
MA3-922 was used in western blot to detect the expression changes of phospholamban in mices treated with ryanodine

Am J Physiol Heart Circ Physiol. 2001 May;280(5):H2046-52.
"Impaired sarcoplasmic reticulum function leads to contractile dysfunction and cardiac hypertrophy."
Author(s): Meyer M, Trost SU, Bluhm WF, Knot HJ, Swanson E, Dillmann WH
Number of Citations: 0
(See PubMed article )
  Ms / Not Cited
MA3-922 was used in western blot to study the effects of increase in L-type calcium channels (LCC) density on calcium signaling in cardiac myocytes overexpressing the alpha (1) subunit of LCC

Circ Res. 2002 Feb 8;90(2):174-81.
"Ca(2+) signaling in cardiac myocytes overexpressing the alpha(1) subunit of L-type Ca(2+) channel."
Author(s): Song LS, Guia A, Muth JN, Rubio M, Wang SQ, Xiao RP, Josephson IR, Lakatta EG, Schwartz A, Cheng H
Number of Citations: 5
(See PubMed article )
  Ms / Not Cited
MA3-922 was used in western blot to investigate the effects of overexpression of the SR calcium pump (SERCA2a) on myocardial contractility in diabetic cardiomyopathy

Diabetes. 2002 Apr;51(4):1166-71.
"Overexpression of the sarcoplasmic reticulum Ca(2+)-ATPase improves myocardial contractility in diabetic cardiomyopathy."
Author(s): Trost SU, Belke DD, Bluhm WF, Meyer M, Swanson E, Dillmann WH
Number of Citations: 17
(See PubMed article )
  Ms / 1:1000
MA3-922 was used in western blot to investigate the calcium handling in cardiomyocytes throughout murine development

Life Sci. 2002 Aug 2;71(11):1279-92.
"Developmental changes of Ca(2+) handling in mouse ventricular cells from early embryo to adulthood."
Author(s): Liu W, Yasui K, Opthof T, Ishiki R, Lee JK, Kamiya K, Yokota M, Kodama I
Number of Citations: 6
(See PubMed article )
  Ms / Not Cited
MA3-922 was used in western blot to study junctin's functions in heart

FASEB J. 2002 Aug;16(10):1310-2.
"Cardiac remodeling and atrial fibrillation in transgenic mice overexpressing junctin."
Author(s): Hong CS, Cho MC, Kwak YG, Song CH, Lee YH, Lim JS, Kwon YK, Chae SW, Kim DH
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
MA3-922 was used in western blot to investigate the contractility of hearts expressing SERCA1a ex vivo and in vivo as well as their response to beta-adrenergic stimulation

Am J Physiol Heart Circ Physiol. 2002 Sep;283(3):H958-65.
"Altered dose response to beta-agonists in SERCA1a-expressing hearts ex vivo and in vivo."
Author(s): Huke S, Prasad V, Nieman ML, Nattamai KJ, Grupp IL, Lorenz JN, Periasamy M
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
MA3-922 was used in western blot to investigate the role of the calcium/calmodulin-dependent kinase II pathway in cardiac disease.

J Biol Chem. 2003 Jul 4;278(27):25063-71.
"Targeted inhibition of Ca2+/calmodulin-dependent protein kinase II in cardiac longitudinal sarcoplasmic reticulum results in decreased phospholamban phosphorylation at threonine 17."
Author(s): Ji Y, Li B, Reed TD, Lorenz JN, Kaetzel MA, Dedman JR
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
MA3-922 was used in western blot to demonstrate the protective effect of nitric oxide on cardiac SL NOSs and sodium/potassium-ATPase against ischemia-induced inactivation.

J Biol Chem. 2003 Oct 24;278(43):41798-803.
"Nitric oxide protects cardiac sarcolemmal membrane enzyme function and ion active transport against ischemia-induced inactivation."
Author(s): Xu KY, Kuppusamy SP, Wang JQ, Li H, Cui H, Dawson TM, Huang PL, Burnett AL, Kuppusamy P, Becker LC
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
MA3-922 was used in western blot to characterize the alterations in sarcoplasmic reticulum calcium homostasis in Ras-induced hypertrophic cardiomyopathy heart

Am J Physiol Heart Circ Physiol. 2004 Jan;286(1):H424-33.
"Sarcoplasmic reticulum calcium defect in Ras-induced hypertrophic cardiomyopathy heart."
Author(s): Zheng M, Dilly K, Dos Santos Cruz J, Li M, Gu Y, Ursitti JA, Chen J, Ross J Jr, Chien KR, Lederer JW, Wang Y
Number of Citations: 1
(See PubMed article )
  Ms / 1:20,000
MA3-922 was used in western blot to investigate the effect of Akt on cardiac morphology and cardiac function.

J Biol Chem. 2003 Nov 28;278(48):47622-8.
"Mechanism of enhanced cardiac function in mice with hypertrophy induced by overexpressed Akt."
Author(s): Kim YK, Kim SJ, Yatani A, Huang Y, Castelli G, Vatner DE, Liu J, Zhang Q, Diaz G, Zieba R, Thaisz J, Drusco A, Croce C, Sadoshima J, Condorelli G, Vatner SF
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
MA3-922 was used in western blot to investigate the effect of chronically decreased SERCA2 calcium pump activity on heart failure in mice

Am J Physiol Heart Circ Physiol. 2004 Mar;286(3):H1146-53.
"Accelerated onset of heart failure in mice during pressure overload with chronically decreased SERCA2 calcium pump activity."
Author(s): Schultz Jel J, Glascock BJ, Witt SA, Nieman ML, Nattamai KJ, Liu LH, Lorenz JN, Shull GE, Kimball TR, Periasamy M
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
MA3-922 was used in western blot to elucidate the functional role of PKA-mediated phosphorylation of cTnI both in vivo and in vitro

Circ Res. 2004 Mar 5;94(4):496-504.
"Frequency- and afterload-dependent cardiac modulation in vivo by troponin I with constitutively active protein kinase A phosphorylation sites."
Author(s): Takimoto E, Soergel DG, Janssen PM, Stull LB, Kass DA, Murphy AM
Number of Citations: 1
(See PubMed article )
  Ms / 1:5,000
MA3-922 was used in western blot to study the role of the sodium/potassium-ATPase alpha2-subunit isoform during muscle contraction

Am J Physiol Cell Physiol. 2004 Nov;287(5):C1300-10.
"The Na(+)-K(+)-ATPase alpha2-subunit isoform modulates contractility in the perinatal mouse diaphragm."
Author(s): Radzyukevich TL, Moseley AE, Shelly DA, Redden GA, Behbehani MM, Lingrel JB, Paul RJ, Heiny JA
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
MA3-922 was used in western blot to investigate the effects of overexpression of SERCA2a on cardiac function and calcium handling in mice

Am J Physiol Heart Circ Physiol. 2004 Nov;287(5):H2164-72.
"Doxycycline inducible expression of SERCA2a improves calcium handling and reverts cardiac dysfunction in pressure overload-induced cardiac hypertrophy."
Author(s): Suarez J, Gloss B, Belke DD, Hu Y, Scott B, Dieterle T, Kim YK, Valencik ML, McDonald JA, Dillmann WH
Number of Citations: 1
(See PubMed article )
  Ms / 0
MA3-922 was used in western blot to investigate the influence of phosphorylation on specific protein interaction with their cognate antibodies

J Mol Cell Cardiol. 2004 Sep;37(3):795-9.
"Phosphorylation-status of phospholamban and calsequestrin modifies their affinity towards commonly used antibodies."
Author(s): Huke S, Periasamy M
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
MA3-922 was used in western blot to investigate the effect of chronic ventricular myocyte-specific overexpression of angiotensin II type 2 receptor on myocyte contractile function

Am J Physiol Heart Circ Physiol. 2005 Jan;288(1):H317-27.
"Chronic ventricular myocyte-specific overexpression of angiotensin II type 2 receptor results in intrinsic myocyte contractile dysfunction."
Author(s): Nakayama M, Yan X, Price RL, Borg TK, Ito K, Sanbe A, Robbins J, Lorell BH
Number of Citations: 1
(See PubMed article )
  Ms / 0
MA3-922 was used in western blot to study the role of thyroid hormone receptor alpha1 in calcium homeostasis and cardiomyocyte function

J Mol Cell Cardiol. 2005 Apr;38(4):655-63.
"Impaired Ca2+ handling and contraction in cardiomyocytes from mice with a dominant negative thyroid hormone receptor alpha1."
Author(s): Tavi P, Sjögren M, Lunde PK, Zhang SJ, Abbate F, Vennström B, Westerblad H
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
MA3-922 was used in western blot to investigate the activation of intracellular signaling pathways by graded degrees of hemodynamic stress

Physiol Genomics. 2005 Sep 21;23(1):18-27.
"Differential activation of stress-response signaling in load-induced cardiac hypertrophy and failure."
Author(s): Rothermel BA, Berenji K, Tannous P, Kutschke W, Dey A, Nolan B, Yoo KD, Demetroulis E, Gimbel M, Cabuay B, Karimi M, Hill JA
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
MA3-922 was used in western blot to investigate the relationship between left ventricular adenylyl cyclase type VI content and mortality after myocardial infarction

Circulation. 2006 Aug 1;114(5):388-96.
"Increased cardiac adenylyl cyclase expression is associated with increased survival after myocardial infarction."
Author(s): Takahashi T, Tang T, Lai NC, Roth DM, Rebolledo B, Saito M, Lew WY, Clopton P, Hammond HK
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
MA3-922 was used in western blot to investigate the thermogenic mechanism of the white adipose tissue in cold-acclimated Ucp1-/- mice.

J Biol Chem. 2006 Oct 20;281(42):31894-908.
"UCP1-independent thermogenesis in white adipose tissue of cold-acclimated Ucp1-/- mice."
Author(s): Ukropec J, Anunciado RP, Ravussin Y, Hulver MW, Kozak LP
Number of Citations: 1
(See PubMed article )
  Ms / 1:2000
MA3-922 was used in immunocytochemistry and western blot to study the expression, localization and turnover of phospholamban and Ca(2+)-ATPase during myoblast differentiation

Am J Physiol Cell Physiol. 2007 Jun;292(6):C2084-94.
"Cellular trafficking of phospholamban and formation of functional sarcoplasmic reticulum during myocyte differentiation."
Author(s): Stenoien DL, Knyushko TV, Londono MP, Opresko LK, Mayer MU, Brady ST, Squier TC, Bigelow DJ
Number of Citations: 8
(See PubMed article )
  Ms / Not Cited
MA3-922 was used in western blot to study how the myosin regulatory light chain E22K mutation affects its functions

FASEB J. 2007 Dec;21(14):3974-85.
"Myosin regulatory light chain E22K mutation results in decreased cardiac intracellular calcium and force transients."
Author(s): Szczesna-Cordary D, Jones M, Moore JR, Watt J, Kerrick WG, Xu Y, Wang Y, Wagg C, Lopaschuk GD
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
MA3-922 was used in western blot to investigate the role of G alpha o on calcium cycling and contractile function in heart

Am J Physiol Heart Circ Physiol. 2008 Mar;294(3):H1335-47.
"Enhanced calcium cycling and contractile function in transgenic hearts expressing constitutively active G alpha o* protein."
Author(s): Zhu M, Gach AA, Liu G, Xu X, Lim CC, Zhang JX, Mao L, Chuprun K, Koch WJ, Liao R, Koren G, Blaxall BC, Mende U
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
MA3-922 was used in western blot to investigate the structural and functional consequences of the R33Q homozygous mutation in cardiac calsequestrin in a homozygous CASQ2 (R33Q/R33Q) knock in mouse model

Circ Res. 2008 Aug 1;103(3):298-306.
"Unexpected structural and functional consequences of the R33Q homozygous mutation in cardiac calsequestrin: a complex arrhythmogenic cascade in a knock in mouse model."
Author(s): Rizzi N, Liu N, Napolitano C, Nori A, Turcato F, Colombi B, Bicciato S, Arcelli D, Spedito A, Scelsi M, Villani L, Esposito G, Boncompagni S, Protasi F, Volpe P, Priori SG
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
MA3-922 was used in western blot to study the role of AE3 and NKCC1 in the expression and localization of cardiac phosphatases.

J Biol Chem. 2008 Nov 14;283(46):31303-14.
"Impaired cardiac contractility in mice lacking both the AE3 Cl-/HCO3- exchanger and the NKCC1 Na+-K+-2Cl- cotransporter: effects on Ca2+ handling and protein phosphatases."
Author(s): Prasad V, Bodi I, Meyer JW, Wang Y, Ashraf M, Engle SJ, Doetschman T, Sisco K, Nieman ML, Miller ML, Lorenz JN, Shull GE
Number of Citations: 1
(See PubMed article )
  Ms / 1:500
MA3-922 was used in western blot to study the phenotype of transgenic rats overexpressing sodium/calcium exchanger .

Am J Physiol Heart Circ Physiol. 2009 Aug;297(2):H590-601.
"Induced overexpression of Na+/Ca2+ exchanger transgene: altered myocyte contractility, [Ca2+]i transients, SR Ca2+ contents, and action potential duration."
Author(s): Wang J, Chan TO, Zhang XQ, Gao E, Song J, Koch WJ, Feldman AM, Cheung JY
Number of Citations: 1
(See PubMed article )
  Ms / 1:1000
MA3-922 was used in western blot to identify therapeutic targets in ventricular arrhythmia and calcium dysregulation

J Cardiovasc Electrophysiol. 2011 Mar;22(3):316-24.
"Prevention of ventricular arrhythmia and calcium dysregulation in a catecholaminergic polymorphic ventricular tachycardia mouse model carrying calsequestrin-2 mutation."
Author(s): Alcalai R, Wakimoto H, Arad M, Planer D, Konno T, Wang L, Seidman JG, Seidman CE, Berul CI
Number of Citations: 0
(See PubMed article )
  Ms / Not Cited
MA3-922 was used in western blot to study the signal transduction pathway of urocortin 2 in mouse ventricular myocytes

Br J Pharmacol. 2011 Jan;162(2):544-56.
"cAMP- and Ca²(+) /calmodulin-dependent protein kinases mediate inotropic, lusitropic and arrhythmogenic effects of urocortin 2 in mouse ventricular myocytes."
Author(s): Yang LZ, Kockskämper J, Khan S, Suarez J, Walther S, Doleschal B, Unterer G, Khafaga M, Mächler H, Heinzel FR, Dillmann WH, Pieske B, Spiess J
Number of Citations: 1
(See PubMed article )
  Ms / 1:1000
MA3-922 was used in western blot to investigate the involvement of ryanodine receptor phosphorylation in ventricular arrhythmias

Circulation. 2010 Dec 21;122(25):2669-79.
"Ryanodine receptor phosphorylation by calcium/calmodulin-dependent protein kinase II promotes life-threatening ventricular arrhythmias in mice with heart failure."
Author(s): van Oort RJ, McCauley MD, Dixit SS, Pereira L, Yang Y, Respress JL, Wang Q, De Almeida AC, Skapura DG, Anderson ME, Bers DM, Wehrens XH
Number of Citations: 10
(See PubMed article )
  Ms / 1:500
MA3-922 was used in western blot to investigate the mechanism for the decrease of the TT LTCC current density in failing ventricular myocytes

Am J Physiol Heart Circ Physiol. 2011 Mar;300(3):H978-88.
"Decrease in the density of t-tubular L-type Ca2+ channel currents in failing ventricular myocytes."
Author(s): Horiuchi-Hirose M, Kashihara T, Nakada T, Kurebayashi N, Shimojo H, Shibazaki T, Sheng X, Yano S, Hirose M, Hongo M, Sakurai T, Moriizumi T, Ueda H, Yamada M
Number of Citations: 1
(See PubMed article )
  Ms / 1:500
MA3-922 was used in western blot to study the effects on Ca(2+) metabolism of therapeutic blockade of angiotensin II type 1 receptors.

Life Sci. 2011 Mar 28;88(13-14):578-85.
"Angiotensin receptor blockade improves the net balance of cardiac Ca(2+) handling-related proteins in sympathetic hyperactivity-induced heart failure."
Author(s): Ferreira JC, Moreira JB, Campos JC, Pereira MG, Mattos KC, Coelho MA, Brum PC
Number of Citations: 5
(See PubMed article )
  Ms / 1:500
MA3-922 was used in immunohistochemistry and western blot to identify the influence of hyperglycemia on ventricular function following myocardial infarction

Cardiovasc Diabetol. 2011 Apr 6;10():26.
"Hyperglycemia can delay left ventricular dysfunction but not autonomic damage after myocardial infarction in rodents."
Author(s): Rodrigues B, Rosa KT, Medeiros A, Schaan BD, Brum PC, De Angelis K, Irigoyen MC
Number of Citations: 8
(See PubMed article )
  Ms / 0
MA3-922 was used in western blot to investigate the role of SERCAs in muscle contractile function

J Physiol. 2011 Dec 15;589(Pt 24):6139-55.
"Slowed relaxation and preserved maximal force in soleus muscles of mice with targeted disruption of the Serca2 gene in skeletal muscle."
Author(s): Sjåland C, Lunde PK, Swift F, Munkvik M, Ericsson M, Lunde M, Boye S, Christensen G, Ellingsen Ø, Sejersted OM, Andersson KB
Number of Citations: 1
(See PubMed article )
  Ms / 1:10000
MA3-922 was used in western blot to study the effects on murine heart structure, function and molecular biology of lifelong exposure to polyphenol A

Toxicol Sci. 2013 May;133(1):174-85.
"Lifelong exposure to bisphenol a alters cardiac structure/function, protein expression, and DNA methylation in adult mice."
Author(s): Patel BB, Raad M, Sebag IA, Chalifour LE
Number of Citations: 1
(See PubMed article )
  Ms / 1:1000
MA3-922 was used in western blot to study the deleterious effect of pharmacological inhibition of thyroid hormone receptor alpha1 on post-ischemic cardiac function in a murine model of myocardial infarction

Mol Cell Biochem. 2013 Jul;379(1-2):97-105.
"Inhibition of thyroid hormone receptor ?1 impairs post-ischemic cardiac performance after myocardial infarction in mice."
Author(s): Mourouzis I, Kostakou E, Galanopoulos G, Mantzouratou P, Pantos C
Number of Citations: 2
(See PubMed article )
  Po / Not Cited
MA3-922 was used in western blot to study the mechanism underlying the early impairment of LV beta-adrenergic responsiveness after myocardial infarction in vivo

Circ Res. 2004 Nov 26;95(11):e85-95.
"Alterations in myofilament function contribute to left ventricular dysfunction in pigs early after myocardial infarction."
Author(s): van der Velden J, Merkus D, Klarenbeek BR, James AT, Boontje NM, Dekkers DH, Stienen GJ, Lamers JM, Duncker DJ
Number of Citations: 1
(See PubMed article )
  Po / 1:500
MA3-922 was used in western blot to study the functions of hESC-CMs in intracellular calcium handling and the importance of SR in the contraction process

Stem Cells. 2006 Feb;24(2):236-45.
"Functional properties of human embryonic stem cell-derived cardiomyocytes: intracellular Ca2+ handling and the role of sarcoplasmic reticulum in the contraction."
Author(s): Dolnikov K, Shilkrut M, Zeevi-Levin N, Gerecht-Nir S, Amit M, Danon A, Itskovitz-Eldor J, Binah O
Number of Citations: 1
(See PubMed article )
  Rb / Not Cited
MA3-922 was used in western blot to study the expression of Ca(2+) regulatory membrane protein isoforms in skeletal, cardiac and neonatal muscle fibres

Biochim Biophys Acta. 2000 Jun 1;1466(1-2):151-68.
"Comparative analysis of the isoform expression pattern of Ca(2+)-regulatory membrane proteins in fast-twitch, slow-twitch, cardiac, neonatal and chronic low-frequency stimulated muscle fibers."
Author(s): Froemming GR, Murray BE, Harmon S, Pette D, Ohlendieck K
Number of Citations: 7
(See PubMed article )
  Rb / Not Cited
MA3-922 was used in western blot and immunohistochemistry to investigate the specific role of S100A1 in the regulation of myocardial contractility.

Proc Natl Acad Sci U S A. 2001 Nov 20;98(24):13889-94.
"S100A1: a regulator of myocardial contractility."
Author(s): Most P, Bernotat J, Ehlermann P, Pleger ST, Reppel M, Börries M, Niroomand F, Pieske B, Janssen PM, Eschenhagen T, Karczewski P, Smith GL, Koch WJ, Katus HA, Remppis A
Number of Citations: 1
(See PubMed article )
  Rb / Not Cited
MA3-922 was used in western blot to study the interaction of increased SERCA2a activity with beta-adrenoceptor stimulation in rabbit myocytes

Am J Physiol Heart Circ Physiol. 2002 Dec;283(6):H2450-7.
"Interaction between increased SERCA2a activity and beta -adrenoceptor stimulation in adult rabbit myocytes."
Author(s): Chaudhri B, Del Monte F, Hajjar RJ, Harding SE
Number of Citations: 1
(See PubMed article )
  Rb / Not Cited
MA3-922 was used in western blot to investigate the molecular basis of the aberrant calcium handling during heart failure.

Am J Physiol Heart Circ Physiol. 2007 Mar;292(3):H1607-18.
"Cellular and molecular determinants of altered Ca2+ handling in the failing rabbit heart: primary defects in SR Ca2+ uptake and release mechanisms."
Author(s): Armoundas AA, Rose J, Aggarwal R, Stuyvers BD, O'rourke B, Kass DA, Marbán E, Shorofsky SR, Tomaselli GF, William Balke C
Number of Citations: 1
(See PubMed article )
  Rb / 1:1000
MA3-922 was used in western blot to investigate the therapeutic effectiveness of NADPH oxidase inhibitor apocynin against heart failure in rabbits

Mol Cell Biochem. 2010 Oct;343(1-2):143-53.
"NADPH oxidase inhibition ameliorates cardiac dysfunction in rabbits with heart failure."
Author(s): Liu Y, Huang H, Xia W, Tang Y, Li H, Huang C
Number of Citations: 1
(See PubMed article )
  Rb / Not Cited
MA3-922 was used in western blot to study the ability of estradiol to promote and progesterone to protect against sudden cardiac death in a rabbit model of long QT type 2

Heart Rhythm. 2012 May;9(5):823-32.
"Estradiol promotes sudden cardiac death in transgenic long QT type 2 rabbits while progesterone is protective."
Author(s): Odening KE, Choi BR, Liu GX, Hartmann K, Ziv O, Chaves L, Schofield L, Centracchio J, Zehender M, Peng X, Brunner M, Koren G
Number of Citations: 1
(See PubMed article )
  Ro / Not Cited
MA3-922 was used in western blot to study mammalian hibernators' inborn cardioprotective functions during hibernation

Am J Physiol Heart Circ Physiol. 2004 Jun;286(6):H2219-28.
"Insights into cardioprotection obtained from study of cellular Ca2+ handling in myocardium of true hibernating mammals."
Author(s): Yatani A, Kim SJ, Kudej RK, Wang Q, Depre C, Irie K, Kranias EG, Vatner SF, Vatner DE
Number of Citations: 1
(See PubMed article )
  Rt / 1:3,000
MA3-922 was used in western blot to investigate the effect of sprint training on calcium regulation and sarcoplasmic reticulum (SR) function in postinfarction rat myocytes

J Appl Physiol. 2000 Jul;89(1):38-46.
"Sprint training normalizes Ca(2+) transients and SR function in postinfarction rat myocytes."
Author(s): Zhang LQ, Zhang XQ, Ng YC, Rothblum LI, Musch TI, Moore RL, Cheung JY
Number of Citations: 2
(See PubMed article )
  Rt / 1:1,000
MA3-922 was used in western blot to investigate the relationship of altered sarcoplasmic reticulum (SR) protein expression with late-onset dysfunction in diabetic rats

Am J Physiol Heart Circ Physiol. 2001 Sep;281(3):H1137-47.
"Altered SR protein expression associated with contractile dysfunction in diabetic rat hearts."
Author(s): Zhong Y, Ahmed S, Grupp IL, Matlib MA
Number of Citations: 5
(See PubMed article )
  Rt / 1:1,000
MA3-922 was used in western blot to study the possible contribution of defects in intracellular calcium signaling cardiomyopathy in streptozotocin (STZ)-induced diabetic rats

Am J Physiol Heart Circ Physiol. 2002 Oct;283(4):H1398-408.
"Defective intracellular Ca(2+) signaling contributes to cardiomyopathy in Type 1 diabetic rats."
Author(s): Choi KM, Zhong Y, Hoit BD, Grupp IL, Hahn H, Dilly KW, Guatimosim S, Lederer WJ, Matlib MA
Number of Citations: 1
(See PubMed article )
  Rt / 1:500
MA3-922 was used in western blot to study the possible relationship between SR calcium loading after isoproterenol and gender as well as its correlation with gender differences in ischemia-reperfusion injury

Am J Physiol Heart Circ Physiol. 2003 Dec;285(6):H2657-62.
"Gender differences in sarcoplasmic reticulum calcium loading after isoproterenol."
Author(s): Chen J, Petranka J, Yamamura K, London RE, Steenbergen C, Murphy E
Number of Citations: 1
(See PubMed article )
  Rt / 0
MA3-922 was used in western blot to evaluate the S100A1 gene transfer for engineering cardiac grafts

J Gene Med. 2004 Apr;6(4):387-94.
"S100A1 gene transfer: a strategy to strengthen engineered cardiac grafts."
Author(s): Remppis A, Pleger ST, Most P, Lindenkamp J, Ehlermann P, Schweda C, Löffler E, Weichenhan D, Zimmermann W, Eschenhagen T, Koch WJ, Katus HA
Number of Citations: 1
(See PubMed article )
  Rt / 1:500
MA3-922 was used in western blot to demonstrate the effect of ACVI on has cAMP-independent gene transcription.

J Biol Chem. 2004 Sep 10;279(37):38797-802.
"Adenylyl cyclase type VI gene transfer reduces phospholamban expression in cardiac myocytes via activating transcription factor 3."
Author(s): Gao MH, Tang T, Guo T, Sun SQ, Feramisco JR, Hammond HK
Number of Citations: 1
(See PubMed article )
  Rt / 1:1,000
MA3-922 was used in western blot to investigate the effects of parvalbumin (Parv) expression and sarco (endo) plasmic reticulum calcium-ATPase (SERCA2a) overexpression in the presence of beta-adrenergic stimulation

Am J Physiol Heart Circ Physiol. 2005 Feb;288(2):H601-12.
"Genetic manipulation of calcium-handling proteins in cardiac myocytes. I. Experimental studies."
Author(s): Coutu P, Metzger JM
Number of Citations: 1
(See PubMed article )
  Rt / 1:500
MA3-922 was used in western blot to investigate the effects of mechanical unloading on cardiomyocytes from doxorubicin-induced cardiomyopathy

J Am Coll Cardiol. 2004 Dec 7;44(11):2239-46.
"Mechanical unloading improves intracellular Ca2+ regulation in rats with doxorubicin-induced cardiomyopathy."
Author(s): Takaseya T, Ishimatsu M, Tayama E, Nishi A, Akasu T, Aoyagi S
Number of Citations: 1
(See PubMed article )
  Rt / 1:2,000
MA3-922 was used in western blot to study the mechanism for the cardioprotective effect of protein kinase C

Am J Physiol Heart Circ Physiol. 2005 Dec;289(6):H2484-90.
"Protein kinase C and preconditioning: role of the sarcoplasmic reticulum."
Author(s): Yamamura K, Steenbergen C, Murphy E
Number of Citations: 1
(See PubMed article )
  Rt / 1:1,000
MA3-922 was used in western blot to study the mechanism for cardiac contractility regulation

J Biol Chem. 2006 Dec 15;281(50):38599-608.
"Identification of a novel phosphorylation site in protein phosphatase inhibitor-1 as a negative regulator of cardiac function."
Author(s): Rodriguez P, Mitton B, Waggoner JR, Kranias EG
Number of Citations: 1
(See PubMed article )
  Rt / 1:1,000
MA3-922 was used in western blot to investigate the role of thyroid hormone on cardiac remodeling and myocardial performance in rat

Eur J Cardiothorac Surg. 2007 Aug;32(2):333-9.
"Thyroid hormone attenuates cardiac remodeling and improves hemodynamics early after acute myocardial infarction in rats."
Author(s): Pantos C, Mourouzis I, Markakis K, Dimopoulos A, Xinaris C, Kokkinos AD, Panagiotou M, Cokkinos DV
Number of Citations: 1
(See PubMed article )
  Rt / 1:3,000
MA3-922 was used in western blot to study how 5-HT affects intracellular calcium handling

Am J Physiol Heart Circ Physiol. 2007 Oct;293(4):H2367-76.
"Serotonin increases L-type Ca2+ current and SR Ca2+ content through 5-HT4 receptors in failing rat ventricular cardiomyocytes."
Author(s): Birkeland JA, Swift F, Tovsrud N, Enger U, Lunde PK, Qvigstad E, Levy FO, Sejersted OM, Sjaastad I
Number of Citations: 1
(See PubMed article )
  Rt / 1:5,000
MA3-922 was used in western blot and immunohistochemistry to study the alteration of calcineurin and protein kinase C signaling functions by thapsigargin and phenylephrine in cardiac myocytes.

Am J Physiol Cell Physiol. 2009 May;296(5):C992-C1002.
"Effects of thapsigargin and phenylephrine on calcineurin and protein kinase C signaling functions in cardiac myocytes."
Author(s): Prasad AM, Inesi G
Number of Citations: 1
(See PubMed article )
  Rt / 1:5000
MA3-922 was used in western blot to study the role of succinate in calcium homeostasis and cardiomyocyte viability

Cell Calcium. 2010 Jan;47(1):37-46.
"Succinate modulates Ca(2+) transient and cardiomyocyte viability through PKA-dependent pathway."
Author(s): Aguiar CJ, Andrade VL, Gomes ER, Alves MN, Ladeira MS, Pinheiro AC, Gomes DA, Almeida AP, Goes AM, Resende RR, Guatimosim S, Leite MF
Number of Citations: 3
(See PubMed article )
  Rt / Not Cited
MA3-922 was used in western blot to investigate the mechanism of intracellular calcium dysregulation caused by endotoxin

Crit Care Med. 2010 Oct;38(10):2031-6.
"Caspase-dependent protein phosphatase 2A activation contributes to endotoxin-induced cardiomyocyte contractile dysfunction."
Author(s): Neviere R, Hassoun SM, Decoster B, Bouazza Y, Montaigne D, Maréchal X, Marciniak C, Marchetti P, Lancel S
Number of Citations: 2
(See PubMed article )
  Rt / Not Cited
MA3-922 was used in western blot to investigate the effect of calcium buffering on extraocular muscles

Exp Eye Res. 2010 Nov;91(5):613-22.
"Superior calcium homeostasis of extraocular muscles."
Author(s): Zeiger U, Mitchell CH, Khurana TS
Number of Citations: 1
(See PubMed article )
  Rt / 1:1000
MA3-922 was used in western blot to investigate the effect of thyroid hormone on remodelling the diabetic myocardium

Mol Cell Biochem. 2010 Dec;345(1-2):161-9.
"Thyroid hormone can favorably remodel the diabetic myocardium after acute myocardial infarction."
Author(s): Kalofoutis C, Mourouzis I, Galanopoulos G, Dimopoulos A, Perimenis P, Spanou D, Cokkinos DV, Singh J, Pantos C
Number of Citations: 1
(See PubMed article )
  Rt / 0
MA3-922 was used in western blot to investigate the effect of cerebral ischemia on myocardial dysfunction and calcium homeostasis

Cell Physiol Biochem. 2010;26(3):421-30.
"Cerebral ischemia elicits aberration in myocardium contractile function and intracellular calcium handling."
Author(s): Sun L, Ai J, Wang N, Zhang R, Li J, Zhang T, Wu W, Hang P, Lu Y, Yang B
Number of Citations: 1
(See PubMed article )
  Rt / 1:2000
MA3-922 was used in immunocytochemistry and western blot to investigate the effect of calcineurin A subunit on SERCA2 expression in myocytes

Am J Physiol Heart Circ Physiol. 2011 Jan;300(1):H173-80.
"Silencing calcineurin A subunit reduces SERCA2 expression in cardiac myocytes."
Author(s): Prasad AM, Inesi G
Number of Citations: 1
(See PubMed article )
  Rt / 1 ug/ml
MA3-922 was used in western blot to investigate the effect of deficiency of ovarian sex hormones on myocardial contractility in rats after ovariectomy

Reprod Biol Endocrinol. 2011 Apr 23;9():54.
"Myocardial contractility is preserved early but reduced late after ovariectomy in young female rats."
Author(s): Paigel AS, Ribeiro RF Jr, Fernandes AA, Targueta GP, Vassallo DV, Stefanon I
Number of Citations: 1
(See PubMed article )
  Rt / 1:500
MA3-922 was used in western blot to investigate the relationship between baroreflex dysfunction and heart diastolic function in rodents

J Card Fail. 2011 Jun;17(6):519-25.
"Baroreflex sensitivity impairment is associated with cardiac diastolic dysfunction in rats."
Author(s): Mostarda C, Moraes-Silva IC, Moreira ED, Medeiros A, Piratello AC, Consolim-Colombo FM, Caldini EG, Brum PC, Krieger EM, Irigoyen MC
Number of Citations: 1
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Ca / Not Cited
MA3-922 was used in immunocytochemistry to investigate the use of the baculovirus/High-Five insect cell system for the recombinant expression of Ca(2+)-ATPase and phospholamban

Protein Expr Purif. 2004 Mar;34(1):56-67.
"Improved expression and characterization of Ca2+-ATPase and phospholamban in High-Five cells."
Author(s): Waggoner JR, Huffman J, Griffith BN, Jones LR, Mahaney JE
Number of Citations: 4
(See PubMed article )
  Hu / 1:2,500
MA3-922 was used in immunocytochemistry to investigate that phospholamban may a good good therapeutic target in human heart failure.

Circulation. 2002 Feb 26;105(8):904-7.
"Targeting phospholamban by gene transfer in human heart failure."
Author(s): del Monte F, Harding SE, Dec GW, Gwathmey JK, Hajjar RJ
Number of Citations: 13
(See PubMed article )
  Ms / Not Cited
MA3-922 was used in immunocytochemistry and western blot to study the expression, localization and turnover of phospholamban and Ca(2+)-ATPase during myoblast differentiation

Am J Physiol Cell Physiol. 2007 Jun;292(6):C2084-94.
"Cellular trafficking of phospholamban and formation of functional sarcoplasmic reticulum during myocyte differentiation."
Author(s): Stenoien DL, Knyushko TV, Londono MP, Opresko LK, Mayer MU, Brady ST, Squier TC, Bigelow DJ
Number of Citations: 8
(See PubMed article )
  Po / Not Cited
MA3-922 was used in immunocytochemistry to evaluate the effect of a novel patch method for myocardial repair

Am J Physiol Heart Circ Physiol. 2004 Aug;287(2):H501-11.
"Autologous stem cell transplantation for myocardial repair."
Author(s): Liu J, Hu Q, Wang Z, Xu C, Wang X, Gong G, Mansoor A, Lee J, Hou M, Zeng L, Zhang JR, Jerosch-Herold M, Guo T, Bache RJ, Zhang J
Number of Citations: 1
(See PubMed article )
  Rt / 1:2000
MA3-922 was used in immunocytochemistry and western blot to investigate the effect of calcineurin A subunit on SERCA2 expression in myocytes

Am J Physiol Heart Circ Physiol. 2011 Jan;300(1):H173-80.
"Silencing calcineurin A subunit reduces SERCA2 expression in cardiac myocytes."
Author(s): Prasad AM, Inesi G
Number of Citations: 1
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Hu / Not Cited
MA3-922 was used in immunohistochemistry to investigate the myogenic differentiation of human mesenchymal stem cells (hMSCs) in the adult murine heart

Circulation. 2002 Jan 1;105(1):93-8.
"Human mesenchymal stem cells differentiate to a cardiomyocyte phenotype in the adult murine heart."
Author(s): Toma C, Pittenger MF, Cahill KS, Byrne BJ, Kessler PD
Number of Citations: 81
(See PubMed article )
  Hu / 1:50
MA3-922 was used in immunohistochemistry to investigate the immunohistochemical features of hexagonally cross-linked tubular arrays myopathy

Neuromuscul Disord. 2010 May;20(5):326-9.
"Calsequestrin and junctin immunoreactivity in hexagonally cross-linked tubular arrays myopathy."
Author(s): Di Blasi C, Blasevich F, Bellafiore E, Mottarelli E, Gibertini S, Zanotti S, Saredi S, Mantegazza R, Morandi L, Mora M
Number of Citations: 0
(See PubMed article )
  Ms / 1:500
MA3-922 was used in immunohistochemistry and western blot to identify the influence of hyperglycemia on ventricular function following myocardial infarction

Cardiovasc Diabetol. 2011 Apr 6;10():26.
"Hyperglycemia can delay left ventricular dysfunction but not autonomic damage after myocardial infarction in rodents."
Author(s): Rodrigues B, Rosa KT, Medeiros A, Schaan BD, Brum PC, De Angelis K, Irigoyen MC
Number of Citations: 8
(See PubMed article )
  Rb / 1:250
MA3-922 was used in immunohistochemistry to study the localization and function of the nitric oxide synthase in heart.

Proc Natl Acad Sci U S A. 1999 Jan 19;96(2):657-62.
"Nitric oxide synthase in cardiac sarcoplasmic reticulum."
Author(s): Xu KY, Huso DL, Dawson TM, Bredt DS, Becker LC
Number of Citations: 35
(See PubMed article )
  Rt / 35 ug/ml
MA3-922 was used in immunohistochemistry to check the existence of ryanodine receptor in junctional SR and corbular SR

J Cell Biol. 1993 Feb;120(4):969-80.
"The Ca2+-release channel/ryanodine receptor is localized in junctional and corbular sarcoplasmic reticulum in cardiac muscle."
Author(s): Jorgensen AO, Shen AC, Arnold W, McPherson PS, Campbell KP
Number of Citations: 31
(See PubMed article )
  Rt / 1:5,000
MA3-922 was used in western blot and immunohistochemistry to investigate the specific role of S100A1 in the regulation of myocardial contractility.

Proc Natl Acad Sci U S A. 2001 Nov 20;98(24):13889-94.
"S100A1: a regulator of myocardial contractility."
Author(s): Most P, Bernotat J, Ehlermann P, Pleger ST, Reppel M, Börries M, Niroomand F, Pieske B, Janssen PM, Eschenhagen T, Karczewski P, Smith GL, Koch WJ, Katus HA, Remppis A
Number of Citations: 1
(See PubMed article )
  Rt / 1:5,000
MA3-922 was used in western blot and immunohistochemistry to study the alteration of calcineurin and protein kinase C signaling functions by thapsigargin and phenylephrine in cardiac myocytes.

Am J Physiol Cell Physiol. 2009 May;296(5):C992-C1002.
"Effects of thapsigargin and phenylephrine on calcineurin and protein kinase C signaling functions in cardiac myocytes."
Author(s): Prasad AM, Inesi G
Number of Citations: 1
(See PubMed article )
Blocking Assay
  Species / Dilution Summary
  Ca / Not Cited
MA3-922 was used in blocking/activating experiment to investigate the role of phospholamban in calcium transport in skeletal and cardiac sarcoplasmic reticulum

J Biol Chem. 1993 Aug 15;268(23):17018-25.
"Comparative studies of cardiac and skeletal sarcoplasmic reticulum ATPases. Effect of a phospholamban antibody on enzyme activation by Ca2+."
Author(s): Cantilina T, Sagara Y, Inesi G, Jones LR
Number of Citations: 15
(See PubMed article )
  Ca / Not Cited
MA3-922 was used in blocking/activating experiment and western blot to investigate the mechanism for the effect of phospholamban on cardiac calcium ATPase

J Biol Chem. 1996 Jun 21;271(25):14964-70.
"Purified, reconstituted cardiac Ca2+-ATPase is regulated by phospholamban but not by direct phosphorylation with Ca2+/calmodulin-dependent protein kinase."
Author(s): Reddy LG, Jones LR, Pace RC, Stokes DL
Number of Citations: 9
(See PubMed article )
  GP / Not Cited
MA3-922 was used in blocking/activating experiment to investigate the effect of phospholamban on the regulation of calcium uptake in myocytes of mammals with beta-adrenergic stimulation

Am J Physiol. 1991 Oct;261(4 Pt 2):H1344-9.
"Phospholamban mediates the beta-adrenergic-enhanced Ca2+ uptake in mammalian ventricular myocytes."
Author(s): Sham JS, Jones LR, Morad M
Number of Citations: 18
(See PubMed article )
  Ms / 0.26 uM
MA3-922 was used in western blot and blocking/activating experiment to investigate the mechanism for the effect of phospholamban on calcium transport ATPase

J Biol Chem. 1993 Jun 5;268(16):11486-8.
"Residues 2-25 of phospholamban are insufficient to inhibit Ca2+ transport ATPase of cardiac sarcoplasmic reticulum."
Author(s): Jones LR, Field LJ
Number of Citations: 4
(See PubMed article )
  Rb / Not Cited
MA3-922 was used in blocking/activating experiment to investigate the role of phospholamban in calcium transport in skeletal and cardiac sarcoplasmic reticulum

J Biol Chem. 1993 Aug 15;268(23):17018-25.
"Comparative studies of cardiac and skeletal sarcoplasmic reticulum ATPases. Effect of a phospholamban antibody on enzyme activation by Ca2+."
Author(s): Cantilina T, Sagara Y, Inesi G, Jones LR
Number of Citations: 15
(See PubMed article )
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