Parvalbumin Antibody

Parvalbumin Polyclonal Antibody for Western blot, IF, IHC (P), IP, ELISA

>> See 1 other antibody for PVALB (PV)
Synonyms:
PV
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Rat (Rt)
Published Species Reactivity: Human (Hu), Mouse (Ms), Not Applicable (N/A), Rat (Rt), Sea urchin (Su)
Immunogen: Purified parvalbumin from rat skeletal muscle.
Ordering Information
Pierce Parvalbumin Antibody
Product #
PA1-933
Size
100 µg
Price
$360.00
Purchase
Add Parvalbumin Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 0.1 µg/ml
Immunofluorescence (IF) 1:100-1:200
Immunohistochemistry (Paraffin) (IHC (P)) 1 µg/ml
Immunoprecipitation (IP) 5 µg/ml
ELISA (ELISA) Assay dependent
Published Applications Dilution
Western Blot (WB) See publications below
Immunohistochemistry (IHC) See publications below
ELISA (ELISA) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Lyophilized
Purification: Antigen affinity chromatography
Storage Buffer: PBS with 15mg/ml BSA
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA1-933 detects parvalbumin protein in human and rat samples.

PA1-933 has successfully been used in Western blot, ELISA, immunoprecipitation and immunohistochemical procedures. By Western blot, this antibody detects a 12 kDa protein representing parvalbumin from rat cerebellum.

Parvalbumin protein is relatively small and, therefore, it is recommended that the electrophoresis be performed using tricine-SDS-PAGE gels and transferred to a nylon membrane.

The PA1-933 immunizing protein corresponds to purified parvalbumin from rat skeletal muscle.
General Information
Parvalbumin (PV) is a calcium binding protein expressed in specific muscle fibers and fast-firing neurons. PV consists of a single, unbranched chain of linked amino acids and belongs to a larger group of EF hand proteins. Studies have demonstrated that parvalbumin acts in the decay of calcium in the contraction/ relaxation cycle of fast twitch muscles. This data has shown a positive correlation between the rate of relaxation and the concentration of parvalbumin.
Parvalbumin is also expressed in a specific population of GABAergic interneurones which are thought to play a role in maintaining the balance between excitation and inhibition in the cortex as well as the hippocampus. In amyotrophic lateral sclerosis (ALS) patents, parvalbumin immunoreactivity is specifically absent from neuron populations lost early in ALS.
Product Images
  • Parvalbumin Antibody (PA1-933) in WB
    PA1-933_1_07232010.jpg

    Western Blot with anti-Parvalbumin Polyclonal Antibody (PA1-933) (PA1-933_1_07232010.jpg)

    Western Blot with anti-Parvalbumin Polyclonal Antibody (PA1-933)

    Western blot of parvalbumin from rat cerebellum extract using PA1-933.
  • Parvalbumin Antibody (PA1-933) in IF
    Parvalbumin_PA1-933_Immunofluorescence_C6-Cells.jpg

    Immunofluorescence with anti-Parvalbumin Polyclonal Antibody (PA1-933) (Parvalbumin_PA1-933_Immunofluorescence_C6-Cells.jpg)

    Immunofluorescence with anti-Parvalbumin Polyclonal Antibody (PA1-933)

    Immunofluorescent analysis of Parvalbumin using Anti-Parvalbumin Polyclonal Antibody (Product# PA1-933) shows staining in C6 Cells. Parvalbumin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Parvalbumin (Product# PA1-933) at a dilution of 1:100 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552, Goat Anti-Rabbit). Images were taken at 60X magnification.
  • Parvalbumin Antibody (PA1-933) in IF
    Parvalbumin_PA1-933_Immunofluorescence_Hela-Cells.jpg

    Immunofluorescence with anti-Parvalbumin Polyclonal Antibody (PA1-933) (Parvalbumin_PA1-933_Immunofluorescence_Hela-Cells.jpg)

    Immunofluorescence with anti-Parvalbumin Polyclonal Antibody (PA1-933)

    Immunofluorescent analysis of Parvalbumin using Anti-Parvalbumin Polyclonal Antibody (Product# PA1-933) shows staining in Hela Cells. Parvalbumin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Parvalbumin (Product# PA1-933) at a dilution of 1:100 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552, Goat Anti-Rabbit). Images were taken at 60X magnification.
  • Parvalbumin Antibody (PA1-933) in IF
    Parvalbumin_PA1-933_Immunofluorescence_U251-Cells.jpg

    Immunofluorescence with anti-Parvalbumin Polyclonal Antibody (PA1-933) (Parvalbumin_PA1-933_Immunofluorescence_U251-Cells.jpg)

    Immunofluorescence with anti-Parvalbumin Polyclonal Antibody (PA1-933)

    Immunofluorescent analysis of Parvalbumin using Anti-Parvalbumin Polyclonal Antibody (Product# PA1-933) shows staining in U251 Cells. Parvalbumin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Parvalbumin (Product# PA1-933) at a dilution of 1:200 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552, Goat Anti-Rabbit). Images were taken at 60X magnification.
  • Parvalbumin Antibody (PA1-933) in IHC
    PA1-933_IHC_Cerebellum_Tissue.jpg

    Immunohistochemistry with anti-Parvalbumin Polyclonal Antibody (PA1-933) (PA1-933_IHC_Cerebellum_Tissue.jpg)

    Immunohistochemistry with anti-Parvalbumin Polyclonal Antibody (PA1-933)

    Immunohistochemistry was performed on normal biopsies of deparaffinized Human cerebellum tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a rabbit polyclonal antibody recognizing Parvalbumin (PA1-933) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  • Parvalbumin Antibody (PA1-933) in IHC
    PA1-933_IHC_skeletal_muscle.jpg

    Immunohistochemistry with anti-Parvalbumin Polyclonal Antibody (PA1-933) (PA1-933_IHC_skeletal_muscle.jpg)

    Immunohistochemistry with anti-Parvalbumin Polyclonal Antibody (PA1-933)

    Immunohistochemistry was performed on normal biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing Parvalbumin (PA1-933) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  • Parvalbumin Antibody (PA1-933) in IHC
    PA1-933_IHC_Tonsil_Tissue.jpg

    Immunohistochemistry with anti-Parvalbumin Polyclonal Antibody (PA1-933) (PA1-933_IHC_Tonsil_Tissue.jpg)

    Immunohistochemistry with anti-Parvalbumin Polyclonal Antibody (PA1-933)

    Immunohistochemistry was performed on normal biopsies of deparaffinized Human tonsil tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a rabbit polyclonal antibody recognizing Parvalbumin (PA1-933) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Publications:
Western Blot
  Species / Dilution Summary
  Hu / 0.05 ug/ml
PA1-933 was used in ELISA and western blot to compare the expression levels of parvalbumin in parahippocampal gyrus in Alzheimer disease patients and normal people

J Neurol Sci. 1992 Jul;110(1-2):57-61.
"Immunoreactive parvalbumin concentrations in parahippocampal gyrus decrease in patients with Alzheimer's disease."
Author(s): Inaguma Y, Shinohara H, Inagaki T, Kato K
Number of Citations: 2
(See PubMed article )
  Rt / 0.06 ug/ml
PA1-933 was used in ELISA and western blot to investigate tissue distribution of parvalbumin in rat and its changes during development

Biochim Biophys Acta. 1991 Sep 2;1075(1):68-74.
"Sensitive immunoassay for rat parvalbumin: tissue distribution and developmental changes."
Author(s): Inaguma Y, Kurobe N, Shinohara H, Kato K
Number of Citations: 3
(See PubMed article )
  Rt / 1:200
PA1-933 was used in western blot to study the effects of ageing on muscle quality and sarcoplasmic reticulum Ca(2+) release.

Acta Physiol (Oxf). 2011 Mar;201(3):391-403.
"Ageing, but not yet senescent, rats exhibit reduced muscle quality and sarcoplasmic reticulum function."
Author(s): Russ DW, Grandy JS, Toma K, Ward CW
Number of Citations: 3
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Ms / Not Cited
PA1-933 was used in immunohistochemistry to study the neuronal plasticity in murine barrel cortex during development

Eur J Neurosci. 2009 Dec 3;30(11):2053-63.
"Parvalbumin-containing neurons, perineuronal nets and experience-dependent plasticity in murine barrel cortex."
Author(s): Nowicka D, Soulsby S, Skangiel-Kramska J, Glazewski S
Number of Citations: 5
(See PubMed article )
  N/A / Not Cited
PA1-933 was used in immunohistochemistry to study the expression and localization of members of the calbindin subfamily in the sea cucumber

PLoS One. 2012;7(3):e32689.
"Calbindin-D32k is localized to a subpopulation of neurons in the nervous system of the sea cucumber Holothuria glaberrima (Echinodermata)."
Author(s): Díaz-Balzac CA, Lázaro-Peña MI, García-Rivera EM, González CI, García-Arrarás JE
Number of Citations: 0
(See PubMed article )
  Rt / Not Cited
PA1-933 was used in immunohistochemistry to detect the expression of Nogo-A protein after ischemic stroke in rat

Stroke. 2008 Jul;39(7):2091-8.
"Nogo-A expression after focal ischemic stroke in the adult rat."
Author(s): Cheatwood JL, Emerick AJ, Schwab ME, Kartje GL
Number of Citations: 1
(See PubMed article )
  Rt / 1:1000
PA1-933 was used in immunohistochemistry to investigate the changes of endonuclease G in thalamic reticular nucleus neurons after ischemia

Exp Brain Res. 2008 Sep;190(1):81-9.
"Endonuclease G expression in thalamic reticular nucleus after global cerebral ischemia."
Author(s): Nielsen M, Zimmer J, Diemer NH
Number of Citations: 1
(See PubMed article )
  Rt / 1:100
PA1-933 was used in immunohistochemistry to investigate the effect of growth factors on neuron proliferation and growth after ischemia

J Neurosurg. 2010 Oct;113(4):835-50.
"Induction of striatal neurogenesis and generation of region-specific functional mature neurons after ischemia by growth factors. Laboratory investigation."
Author(s): Yoshikawa G, Momiyama T, Oya S, Takai K, Tanaka J, Higashiyama S, Saito N, Kirino T, Kawahara N
Number of Citations: 0
(See PubMed article )
  Rt / Not Cited
PA1-933 was used in immunohistochemistry to study the ataxic Guillain-Barr syndrome and acute sensory ataxic neuropathy

J Neurol Neurosurg Psychiatry. 2011 Mar;82(3):294-9.
"Ataxic Guillain-Barré syndrome and acute sensory ataxic neuropathy form a continuous spectrum."
Author(s): Ito M, Matsuno K, Sakumoto Y, Hirata K, Yuki N
Number of Citations: 1
(See PubMed article )
  Su / 0.2 ug/ml
PA1-933 was used in immunohistochemistry to investigate the central nervous system of sea cucumber

Glia. 2010 Oct;58(13):1581-93.
"Organization of glial cells in the adult sea cucumber central nervous system."
Author(s): Mashanov VS, Zueva OR, Garcia-Arraras JE
Number of Citations: 1
(See PubMed article )
ELISA
  Species / Dilution Summary
  Hu / Not Cited
PA1-933 was used in ELISA and western blot to compare the expression levels of parvalbumin in parahippocampal gyrus in Alzheimer disease patients and normal people

J Neurol Sci. 1992 Jul;110(1-2):57-61.
"Immunoreactive parvalbumin concentrations in parahippocampal gyrus decrease in patients with Alzheimer's disease."
Author(s): Inaguma Y, Shinohara H, Inagaki T, Kato K
Number of Citations: 2
(See PubMed article )
  Rt / Not Cited
PA1-933 was used in ELISA and western blot to investigate tissue distribution of parvalbumin in rat and its changes during development

Biochim Biophys Acta. 1991 Sep 2;1075(1):68-74.
"Sensitive immunoassay for rat parvalbumin: tissue distribution and developmental changes."
Author(s): Inaguma Y, Kurobe N, Shinohara H, Kato K
Number of Citations: 3
(See PubMed article )
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