Parkin Antibody

Parkin Polyclonal Antibody for Western blot, IHC

>> See 12 other antibodies for PARK2 (Ubiquitin E3 ligase PRKN, Parkinson juvenile disease protein 2, Parkinson disease protein 2, Prkn, Park2)
Synonyms:
Ubiquitin E3 ligase PRKN, Parkinson juvenile disease protein 2, Parkinson disease protein 2, Prkn, Park2
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Rat (Rt)
Published Species Reactivity: Human (Hu), Rat (Rt)
Immunogen: Synthetic Peptide: C I(298) K E L H H F R I L G E E Q Y N(313)
Ordering Information
Pierce Parkin Antibody
Product #
PA1-751
Size
100 µg
Price
$316.00
Purchase
Add Parkin Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 0.5 µg/ml
Immunohistochemistry (IHC) 1.0 µg/ml
Published Applications Dilution
Western Blot (WB) See publications below
Immunoprecipitation (IP) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Antigen affinity chromatography
Storage Buffer: PBS with 1mg/ml BSA
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA1-751 detects recombinant human parkin.

PA1-751 has been successfully used in Western blot and immunohistochemical procedures. By Western blot, this antibody detects an ~52 kDa protein representing recombinant human parkin.

The PA1-751 immunizing peptide corresponds to amino acid residues 298-313 from human parkin. This sequence is completely conserved between human, mouse and rat. PA1-751 immunizing peptide (Cat. # PEP-159) is available for use in neutralization and control experiments.

General Information
Parkinson’s Disease, the second most common neurodegenerative disease after Alzheimer’s Disease, is characterized by the loss of dopaminergic neurons and the presence of Lewy bodies (comprised of alpha-synuclein and parkin inclusions). Autosomal Recessive Juvenile Parkinsonism (AR-JP) is a recently described form of Parkinson’s Disease that has been linked to a gene that codes for parkin. Parkin, a 52 kDa protein, has a suggested role in the ubiquitin/proteasome pathway for protein degradation. The amino terminus bears sequence homology to ubiquitin while functionally it acts as a RING-type ubiquitin protein ligase (E3) that coordinates the transfer of ubiquitin to substrate proteins, thus targeting them for degradation by the proteasome.

Studies show that parkin interacts with and ubiquitinates the alpha-synuclein interacting protein, synphilin-1. Recent analysis of parkin mutations in AR-JP patients reveals that parkin becomes functionally inactive as an E3 enzyme. This suggests that the loss of function mutation within parkin results in a failure of ubiquitin/proteasome-mediated proteolysis of synphilin-1 and the accumulation of proteins within the cell resulting in neuronal cell death.
Product Images
  • Parkin Antibody (PA1-751) in IHC
    Park2_Antibody_PA1-751_Immunohistochemical(IHC).jpg

    Immunohistochemistry with anti-Parkin Polyclonal Antibody (PA1-751) (Park2_Antibody_PA1-751_Immunohistochemical(IHC).jpg)

    Immunohistochemistry with anti-Parkin Polyclonal Antibody (PA1-751)

    Immunolocalization of parkin in the pedunculopontine tegmental nucleus in rat brain using PA1-751.
Publications:
Western Blot
  Species / Dilution Summary
  Rt / Not Cited
PA1-751 was used in immunoprecipitation and western blot to investigate the interaction between parkin and CASK and their distribution in cultured cortical neurons and rat brain

J Biol Chem. 2002 Jan 4;277(1):486-91.
"Parkin and CASK/LIN-2 associate via a PDZ-mediated interaction and are co-localized in lipid rafts and postsynaptic densities in brain."
Author(s): Fallon L, Moreau F, Croft BG, Labib N, Gu WJ, Fon EA
Number of Citations: 1
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Hu / Not Cited
PA1-751 was used in immunoprecipitation to study the role of parkin ubiquitinated synphilin-1 in lewy-body formation in Parkinson disease

Nat Med. 2001 Oct;7(10):1144-50.
"Parkin ubiquitinates the alpha-synuclein-interacting protein, synphilin-1: implications for Lewy-body formation in Parkinson disease."
Author(s): Chung KK, Zhang Y, Lim KL, Tanaka Y, Huang H, Gao J, Ross CA, Dawson VL, Dawson TM
Number of Citations: 1
(See PubMed article )
  Rt / Not Cited
PA1-751 was used in immunoprecipitation and western blot to investigate the interaction between parkin and CASK and their distribution in cultured cortical neurons and rat brain

J Biol Chem. 2002 Jan 4;277(1):486-91.
"Parkin and CASK/LIN-2 associate via a PDZ-mediated interaction and are co-localized in lipid rafts and postsynaptic densities in brain."
Author(s): Fallon L, Moreau F, Croft BG, Labib N, Gu WJ, Fon EA
Number of Citations: 1
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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