* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
0.03% sodium azide
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Specific Information
MA5-15560 targets PTEN in ICC, IF, FACS, and WB applications and shows reactivity with Human and mouse samples.
The MA5-15560 immunogen is purified recombinant fragment of PTEN expressed in E. Coli.
MA5-15560 detects PTEN which has a predicted molecular weight of approximately 54kDa.
This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway.
PTEN Antibody (MA5-15560) in WB PTEN_Antibody_MA5-15560_Westernblot1-1252013.jpg
Western Blot with anti-PTEN Monoclonal Antibody [1B8] (MA5-15560)
Immunofluorescence analysis of HeLa (left) and HepG2 (right) cells using PTEN monoclonal antibody (Product # MA5-15560) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with DY-554 phalloidin.
PTEN Antibody (MA5-15560) in FACS PTEN_Antibody_MA5-15560_FlowCytometry-1252013.jpg
Flow Cytometry with anti-PTEN Monoclonal Antibody [1B8] (MA5-15560)