PTEN Antibody (1B8)

PTEN Monoclonal Antibody for Western blot, IF, ICC, FACS

>> See 8 other antibodies for PTEN (Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, MMAC1, TEP1, Phosphatase and tensin homolog)
Synonyms:
Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, MMAC1, TEP1, Phosphatase and tensin homolog
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: 1B8
Tested Species Reactivity: Human (Hu), Mouse (Ms)
Immunogen: Purified recombinant fragment of PTEN expressed in E. Coli.
Ordering Information
Pierce PTEN Antibody (1B8)
Product #
MA5-15560
Size
100 ul
Price
$316.00
Purchase
Add PTEN Antibody (1B8) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1/500 - 1/2000
Immunofluorescence (IF) 1:200-1:1000
Immunocytochemistry (ICC) 1:200-1:1000
Flow Cytometry (FACS) 1/200 - 1/400
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Purification: Protein G
Storage Buffer: PBS with 50% glycerol
Preservative: 0.03% sodium azide
Storage Conditions: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Specific Information
MA5-15560 targets PTEN in ICC, IF, FACS, and WB applications and shows reactivity with Human and mouse samples.

The MA5-15560 immunogen is purified recombinant fragment of PTEN expressed in E. Coli.

MA5-15560 detects PTEN which has a predicted molecular weight of approximately 54kDa.
General Information
This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway.
Product Images
  • PTEN Antibody (MA5-15560) in WB
    PTEN_Antibody_MA5-15560_Westernblot1-1252013.jpg

    Western Blot with anti-PTEN Monoclonal Antibody [1B8] (MA5-15560) (PTEN_Antibody_MA5-15560_Westernblot1-1252013.jpg)

    Western Blot with anti-PTEN Monoclonal Antibody [1B8] (MA5-15560)

    Western blot analysis of PTEN using PTEN monoclonal antibody (Product # MA5-15560) in HeLa (1) and NIH/3T3 (2) cell lysate.
  • PTEN Antibody (MA5-15560) in IF
    PTEN_Antibody_MA5-15560_Immunofluorescence1-1252013.jpg

    Immunofluorescence with anti-PTEN Monoclonal Antibody [1B8] (MA5-15560) (PTEN_Antibody_MA5-15560_Immunofluorescence1-1252013.jpg)

    Immunofluorescence with anti-PTEN Monoclonal Antibody [1B8] (MA5-15560)

    Immunofluorescence analysis of HeLa (left) and HepG2 (right) cells using PTEN monoclonal antibody (Product # MA5-15560) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with DY-554 phalloidin.
  • PTEN Antibody (MA5-15560) in FACS
    PTEN_Antibody_MA5-15560_FlowCytometry-1252013.jpg

    Flow Cytometry with anti-PTEN Monoclonal Antibody [1B8] (MA5-15560) (PTEN_Antibody_MA5-15560_FlowCytometry-1252013.jpg)

    Flow Cytometry with anti-PTEN Monoclonal Antibody [1B8] (MA5-15560)

    Flow cytometric analysis of HeLa cells using PTEN monoclonal antibody (Product # MA5-15560) (right) and negative control (left).
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific