PSAP Antibody (4D5F4)

PSAP Monoclonal Antibody for Western blot, IF, ICC, IHC, FACS

>> See 3 other antibodies for PSAP
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: 4D5F4
Tested Species Reactivity: Human (Hu)
Immunogen: Purified recombinant fragment of human PSAP (amino acids: 325-524 ) expressed in E. Coli.
Ordering Information
Pierce PSAP Antibody (4D5F4)
Product #
MA5-17159
Size
100 ug
Price
$310.00
Purchase
Add PSAP Antibody (4D5F4) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:500 - 1:2000
Immunofluorescence (IF) 1:50 - 500
Immunocytochemistry (ICC) 1:50-1:500
Immunohistochemistry (IHC) 1:200 - 1:1000
Flow Cytometry (FACS) 1:200 - 1:400
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: purified
Storage Buffer: PBS with 0.5% proprietary stabilizer
Preservative: 0.05% sodium azide
Storage Conditions: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Specific Information
MA5-17159 targets PSAP in FACS, ICC, IHC, IF and WB applications and shows reactivity with Human samples.

The MA5-17159 immunogen is purified recombinant fragment of human PSAP (amino acids: 325-524 ) expressed in E. Coli.

MA5-17159 detects PSAP which has a predicted molecular weight of approximately 58.1kDa.
General Information
This gene encodes a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in this gene have been associated with Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy. Alternative splicing results in multiple transcript variants encoding different isoforms.
Product Images
  • PSAP Antibody (MA5-17159) in WB
    PSAP_Antibody_MA5-17159_Westernblot1-1252013.jpg

    Western Blot with anti-PSAP Monoclonal Antibody [4D5F4] (MA5-17159) (PSAP_Antibody_MA5-17159_Westernblot1-1252013.jpg)

    Western Blot with anti-PSAP Monoclonal Antibody [4D5F4] (MA5-17159)

    Western blot analysis of PSAP using a PSAP monoclonal antibody (Product # MA5-17159) against a human PSAP recombinant protein.
  • PSAP Antibody (MA5-17159) in WB
    PSAP_Antibody_MA5-17159_Westernblot2-1252013.jpg

    Western Blot with anti-PSAP Monoclonal Antibody [4D5F4] (MA5-17159) (PSAP_Antibody_MA5-17159_Westernblot2-1252013.jpg)

    Western Blot with anti-PSAP Monoclonal Antibody [4D5F4] (MA5-17159)

    Western blot analysis of PSAP using PSAP monoclonal antibody (Product # MA5-17159) in HEK293 (1) and PSAP (AA: 325-524) human IgG Fc transfected HEK293 (2) cell lysate.
  • PSAP Antibody (MA5-17159) in IF
    PSAP_Antibody_MA5-17159_Immunofluorescence1-1252013.jpg

    Immunofluorescence with anti-PSAP Monoclonal Antibody [4D5F4] (MA5-17159) (PSAP_Antibody_MA5-17159_Immunofluorescence1-1252013.jpg)

    Immunofluorescence with anti-PSAP Monoclonal Antibody [4D5F4] (MA5-17159)

    Immunofluorescence analysis of HepG2 cells using PSAP monoclonal antibody (Product # MA5-17159) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.
  • PSAP Antibody (MA5-17159) in IHC
    PSAP_Antibody_MA5-17159_Immunohistochemical1-1252013.jpg

    Immunohistochemistry with anti-PSAP Monoclonal Antibody [4D5F4] (MA5-17159) (PSAP_Antibody_MA5-17159_Immunohistochemical1-1252013.jpg)

    Immunohistochemistry with anti-PSAP Monoclonal Antibody [4D5F4] (MA5-17159)

    Immunohistochemical analysis of paraffin-embedded pancreas tissues using PSAP monoclonal antibody (Product # MA5-17159) followed with DAB staining.
  • PSAP Antibody (MA5-17159) in FACS
    PSAP_Antibody_MA5-17159_FlowCytometry-1252013.jpg

    Flow Cytometry with anti-PSAP Monoclonal Antibody [4D5F4] (MA5-17159) (PSAP_Antibody_MA5-17159_FlowCytometry-1252013.jpg)

    Flow Cytometry with anti-PSAP Monoclonal Antibody [4D5F4] (MA5-17159)

    Flow cytometric analysis of HeLa cells using PSAP monoclonal antibody (Product # MA5-17159) (green) and negative control (purple).
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific