* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Antigen affinity chromatography
0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Product Specific Information
PA5-26006 targets PRL in IF, IHC, and WB applications and shows reactivity with Human samples.
The PA5-26006 immunogen is synthetic peptide from the S163 region of human PRL conjugated to KLH.
Prolactin (PRL) is a hormone involved in a variety of important functions including ion transport and osmoregulation, stimulation of milk, protein synthesis as well as the regulation of numerous reproductive functions. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor, a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. The PRL receptor consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.
PRL Antibody (PA5-26006) in WB PRL_Antibody_PA5-26006_WB_1_112013.jpg
Western Blot with anti-PRL Polyclonal Antibody (PA5-26006)
Immunofluorescent analysis of Hela cells using anti-PRL Polyclonal Antibody (PA5-26006) at a dilution of 1:10-50. Primary antibody was detected with goat anti-rabbit lgG, Alexa Fluor 488 conjugated secondary antibody (green). DAPI was used to stain the cells nuclear (blue).
PRL Antibody (PA5-26006) in IHC PRL_Antibody_PA5-26006_IHC_1_112013.jpg
Immunohistochemistry with anti-PRL Polyclonal Antibody (PA5-26006)
Immunohistochemical staining of formalin-fixed and paraffin-embedded human breast carcinoma tissue using anti-PRL Polyclonal Antibody (PA5-26006), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)