PRK2 Antibody (1D1)

PRK2 Monoclonal Antibody for Western blot, IHC, FACS

>> See 3 other antibodies for PKN2 (RP5-905H16.1, PAK2, PRK2, PRKCL2, PRO2042, Pak-2, cardiolipin-activated protein kinase Pak2; protein kinase C-like 2; protein-kinase C-related kinase 2; serine/threonine-protein kinase N2)
Synonyms:
RP5-905H16.1, PAK2, PRK2, PRKCL2, PRO2042, Pak-2, cardiolipin-activated protein kinase Pak2; protein kinase C-like 2; protein-kinase C-related kinase 2; serine/threonine-protein kinase N2
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: 1D1
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt), Non-human primate (Nhp)
Immunogen: Purified recombinant fragment of human PRK2 expressed in E. Coli.
Ordering Information
Pierce PRK2 Antibody (1D1)
Product #
MA5-15887
Size
100 ul
Price
$316.00
Purchase
Add PRK2 Antibody (1D1) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1/500 - 1/2000
Immunohistochemistry (IHC) 1/200 - 1/1000
Flow Cytometry (FACS) 1/200 - 1/400
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Storage Buffer: ascites
Preservative: 0.03% sodium azide
Storage Conditions: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Specific Information
MA5-15887 targets PRK2 in FACS, IHC, and WB applications and shows reactivity with Human, mouse, Non-human primate, and Rat samples.

The MA5-15887 immunogen is purified recombinant fragment of human PRK2 expressed in E. Coli. .

MA5-15887 detects PRK2 which has a predicted molecular weight of approximately 140kDa.
General Information
Protein-kinase-C-related kinases (PRKs) are part of the lipid-regulated protein kinases (PKC) which also include liver PAK & PKN. Human PRK1 and PRK2 share structurally similar catalytic domains, but less similar N-terminal regulatory regions suggesting different regulatory domain functions. PRK1 and PRK2, as well as a third member of this family, PRK3, show distinct patterns of expression in adult tissues. Additionally, the serine-threonine kinase PRK2 can be specifically cleaved by caspase-3 (and/or caspase-3-like subfamily members) during apoptosis.
Product Images
  • PRK2 Antibody (MA5-15887) in WB
    PRK2_Antibody_MA5-15887_Westernblot1-1252013.jpg

    Western Blot with anti-PRK2 Monoclonal Antibody [1D1] (MA5-15887) (PRK2_Antibody_MA5-15887_Westernblot1-1252013.jpg)

    Western Blot with anti-PRK2 Monoclonal Antibody [1D1] (MA5-15887)

    Western blot analysis of PRK2 using a PRK2 monoclonal antibody (Product # MA5-15887) against a human PRK2 (AA: 555-718) recombinant protein.
  • PRK2 Antibody (MA5-15887) in WB
    PRK2_Antibody_MA5-15887_Westernblot2-1252013.jpg

    Western Blot with anti-PRK2 Monoclonal Antibody [1D1] (MA5-15887) (PRK2_Antibody_MA5-15887_Westernblot2-1252013.jpg)

    Western Blot with anti-PRK2 Monoclonal Antibody [1D1] (MA5-15887)

    Western blot analysis of PRK2 using PRK2 monoclonal antibody (Product # MA5-15887) in PC-12 (1), COS-7 (2), K562 (3), Jurkat (4), HeLa (5), A431 (6), C6 (7), NIH/3T3 (8) and HEK293 (9) cell lysate.
  • PRK2 Antibody (MA5-15887) in IHC
    PRK2_Antibody_MA5-15887_Immunohistochemical2-1252013.jpg

    Immunohistochemistry with anti-PRK2 Monoclonal Antibody [1D1] (MA5-15887) (PRK2_Antibody_MA5-15887_Immunohistochemical2-1252013.jpg)

    Immunohistochemistry with anti-PRK2 Monoclonal Antibody [1D1] (MA5-15887)

    Immunohistochemical analysis of paraffin-embedded lung cancer tissues using PRK2 monoclonal antibody (Product # MA5-15887) followed with DAB staining.
  • PRK2 Antibody (MA5-15887) in IHC
    PRK2_Antibody_MA5-15887_Immunohistochemical1-1252013.jpg

    Immunohistochemistry with anti-PRK2 Monoclonal Antibody [1D1] (MA5-15887) (PRK2_Antibody_MA5-15887_Immunohistochemical1-1252013.jpg)

    Immunohistochemistry with anti-PRK2 Monoclonal Antibody [1D1] (MA5-15887)

    Immunohistochemical analysis of paraffin-embedded prostate tissues using PRK2 monoclonal antibody (Product # MA5-15887) followed with DAB staining.
  • PRK2 Antibody (MA5-15887) in FACS
    PRK2_Antibody_MA5-15887_FlowCytometry-1252013.jpg

    Flow Cytometry with anti-PRK2 Monoclonal Antibody [1D1] (MA5-15887) (PRK2_Antibody_MA5-15887_FlowCytometry-1252013.jpg)

    Flow Cytometry with anti-PRK2 Monoclonal Antibody [1D1] (MA5-15887)

    Flow cytometric analysis of NIH/3T3 cells using PRK2 monoclonal antibody (Product # MA5-15887) (blue) and negative control (red).
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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