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PMCA ATPase Monoclonal Antibody (5F10)

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PMCA ATPase Antibody for ICC, IF, IHC (P, F), IP, Western blot

>> See 1 other antibody for ATP2B1 (Plasma Membrane Calcium Pump ATPase)

Synonyms:

Plasma Membrane Calcium Pump ATPase

Entrez Gene ID:

(Human) 490

UniProt ID:

P20020

Details

Host / Isotype:	Mouse / IgG2a
Class:	Monoclonal
Type:	Antibody
Clone:	5F10
Species Reactivity:	Human (Hu) Murine (Ms) Rat (Rt) Canine (Ca) Rabbit (Rb) Non-human primate (Nhp) Ovine (Ov) Feline (Fe) Chicken (Ck) Amphibian (Am)
Immunogen:	Purified human erythrocyte calcium ATPase.

Ordering Information
Pierce PMCA ATPase Monoclonal Antibody (5F10)
Product Number	Pkg. Size	Price	Purchase

MA3-914	100 µl	$354.00	Qty:

Storage:	-20º C, Avoid Freeze/Thaw Cycles
Form:	100 µl of PBS-diluted ascites containing 0.05% sodium azide.

Applications	Dilution *

Western Blot (WB)	1:1,000
Immunocytochemistry (ICC)	Assay dependent
Immunofluorescence (IF)	Assay dependent
Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))	1:500
Immunoprecipitation (IP)	Assay dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Product Specific Information

MA3-914 detects calcium pump of the plasma membrane (PMCA) ATPase from amphibian, canine, chicken, feline, human, mouse, sheep, primate, rabbit, and rat tissues. This antibody detects all four known isoforms of the PMCA ATPase.

MA3-914 has been successfully used in Western blot, immunohistochemistry, immunocytochemistry, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody recognizes an ~140 kDa protein representing PMCA ATPase and bands at 95kDa and 180 kDa which probably represent products of aggregation and/or natural proteolytic products of the pump from rat liver membrane preparations. Immunohistochemical staining of PMCA ATPase with MA3-914 yields a pattern consistent with that seen in the literature and depends on the tissue being studied and the localization of the isoforms present.

MA3-914 can be used on both frozen and formalin-fixed paraffin-embedded tissues.

The MA3-914 antigen is purified human erythrocyte calcium ATPase. This antibody recognizes an epitope between amino acids 724-783 of the human erythrocyte calcium pump.

General Information

The calcium pump of the plasma membrane (PMCA ATPase) pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to a number of other ATPases including the sarcoplasmic and endoplasmic reticulum calcium (SERCA) ATPase and the sodium/potassium pump. Studies indicate that there are four known isoforms of the PMCA ATPase which are encoded by a multigene family.

PubMed References:

BLOCK (1)
ELISA (2)
ICC (12)
IHC (12)
IP (10)
WB (54)

Blocking Assay
	Species / Dilution	Summary
	Rt / Not Cited	MA3-914 was used in blocking/activating experiment, immunohistochemistry and western blot to investigate the distribution of calcium ATPase and 28-kDa CaBP in rat kidney distal convoluted tubule principal cells Am J Physiol. 1989 Nov;257(5 Pt 2):F842-9. "Plasma membrane calcium pump and 28-kDa calcium binding protein in cells of rat kidney distal tubules." Author(s): Borke JL, Caride A, Verma AK, Penniston JT, Kumar R Number of Citations: 9 (See PubMed article )

ELISA
	Species / Dilution	Summary
	Hu / 1:60,000	MA3-914 was used in western blot and ELISA to investigate the epitopes and isoform specificity of anti-hPMCA antibodies and to study hPMCA4's distribution and its alternative splicing variants in different human tissues Biochem J. 1996 May 15;316 ( Pt 1)():353-9. "Detection of isoform 4 of the plasma membrane calcium pump in human tissues by using isoform-specific monoclonal antibodies." Author(s): Caride AJ, Filoteo AG, Enyedi A, Verma AK, Penniston JT Number of Citations: 13 (See PubMed article )
	Rt / Not Cited	MA3-914 was used in western blot and ELISA to produce and characterize novel anti-PMCA antibodies and to investigate the expression and distribution of PMCA isoforms in rat brain J Biol Chem. 1997 Sep 19;272(38):23741-7. "Plasma membrane Ca2+ pump in rat brain. Patterns of alternative splices seen by isoform-specific antibodies." Author(s): Filoteo AG, Elwess NL, Enyedi A, Caride A, Aung HH, Penniston JT Number of Citations: 21 (See PubMed article )

Immunocytochemistry
	Species / Dilution	Summary
	Am / Not Cited	MA3-914 was used in immunocytochemistry and western blot to investigate the mechanisms for plasma membrane calcium-ATPase (PMCA) internalization during oocyte maturation Dev Biol. 2008 Dec 1;324(1):99-107. "Internalization of plasma membrane Ca2+-ATPase during Xenopus oocyte maturation." Author(s): El-Jouni W, Haun S, Machaca K Number of Citations: 1 (See PubMed article )
	Ch / 1:200	MA3-914 was used in immunocytochemistry to locate plasmalemma calcium/hydrogen-ATPase (PMCA pump) in isolated retina J Physiol. 2004 Nov 1;560(Pt 3):639-57. "Neurotransmitter modulation of extracellular H+ fluxes from isolated retinal horizontal cells of the skate." Author(s): Molina AJ, Verzi MP, Birnbaum AD, Yamoah EN, Hammar K, Smith PJ, Malchow RP Number of Citations: 1 (See PubMed article )
	Hm / Not Cited	MA3-914 was used in immunocytochemistry to identify the gene segments in the cystic fibrosis transmembrane conductance regulator important for its assembly and stability. J Biol Chem. 2001 Jan 12;276(2):1291-8. "Localization of sequences within the C-terminal domain of the cystic fibrosis transmembrane conductance regulator which impact maturation and stability." Author(s): Gentzsch M, Riordan JR Number of Citations: 1 (See PubMed article )
	Hu / Not Cited	MA3-914 was used in immunocytochemistry and western blot to investigate the regulation of calcium pumping from hair cell stereocilia by PMCA J Neurosci. 1998 Jan 15;18(2):610-24. "Plasma membrane Ca2+-ATPase extrudes Ca2+ from hair cell stereocilia." Author(s): Yamoah EN, Lumpkin EA, Dumont RA, Smith PJ, Hudspeth AJ, Gillespie PG Number of Citations: 42 (See PubMed article )
	Hu / Not Cited	MA3-914 was used in immunocytochemistry to investigate the important roles of action cytoskeleton in T-cell activation and its impact on calcium-NFAT signaling Mol Cell Biol. 2004 Feb;24(4):1628-39. "Actin cytoskeleton regulates calcium dynamics and NFAT nuclear duration." Author(s): Rivas FV, O'Keefe JP, Alegre ML, Gajewski TF Number of Citations: 11 (See PubMed article )
	Hu / Not Cited	MA3-914 was used in immunocytochemistry and western blot to investigate the effect of maitotoxin during the convertion of plasmalemmal calcium pump into a calcium-permeable nonselective cation channel Am J Physiol Cell Physiol. 2009 Dec;297(6):C1533-43. "Maitotoxin converts the plasmalemmal Ca(2+) pump into a Ca(2+)-permeable nonselective cation channel." Author(s): Sinkins WG, Estacion M, Prasad V, Goel M, Shull GE, Kunze DL, Schilling WP Number of Citations: 1 (See PubMed article )
	Ms / 1:100	MA3-914 was used in immunocytochemistry, immunoprecipitation and western blot to investigate the expression of caclium-ATPase and NCX1 sodium/caclium exchanger in distal convoluted tubule cells Am J Physiol Renal Physiol. 2002 Jul;283(1):F29-40. "Plasma membrane Ca2+-ATPase and NCX1 Na+/Ca2+ exchanger expression in distal convoluted tubule cells." Author(s): Magyar CE, White KE, Rojas R, Apodaca G, Friedman PA Number of Citations: 1 (See PubMed article )
	Ms / Not Cited	MA3-914 was used in immunocytochemistry to locate plasmalemma calcium/hydrogen-ATPase (PMCA pump) in isolated retina J Physiol. 2004 Nov 1;560(Pt 3):639-57. "Neurotransmitter modulation of extracellular H+ fluxes from isolated retinal horizontal cells of the skate." Author(s): Molina AJ, Verzi MP, Birnbaum AD, Yamoah EN, Hammar K, Smith PJ, Malchow RP Number of Citations: 1 (See PubMed article )
	Ms / Not Cited	MA3-914 was used in immunocytochemistry to study the binding of Bcr to the apical PDZK1 protein and to the Golgi-localized Mint3. J Cell Sci. 2004 Nov 1;117(Pt 23):5535-41. "Bcr (breakpoint cluster region) protein binds to PDZ-domains of scaffold protein PDZK1 and vesicle coat protein Mint3." Author(s): Malmberg EK, Andersson CX, Gentzsch M, Chen JH, Mengos A, Cui L, Hansson GC, Riordan JR Number of Citations: 1 (See PubMed article )
	Ms / 1:500	MA3-914 was used in immunocytochemistry to investigate specific plasma membrane calcium pumps in mouse olfactory sensory neurons. Chem Senses. 2006 Oct;31(8):725-30. "Plasma membrane calcium pumps in mouse olfactory sensory neurons." Author(s): Weeraratne SD, Valentine M, Cusick M, Delay R, Van Houten JL Number of Citations: 1 (See PubMed article )
	Nhp / Not Cited	MA3-914 was used in immunoprecipitation and immunocytochemistry to investigate the role of plasma-membrane calcium-ATPase during the mechanotransduction in sensory hair cells. J Neurosci. 2006 Jun 7;26(23):6172-80. "Splice-site A choice targets plasma-membrane Ca2+-ATPase isoform 2 to hair bundles." Author(s): Hill JK, Williams DE, LeMasurier M, Dumont RA, Strehler EE, Gillespie PG Number of Citations: 1 (See PubMed article )
	Rt / Not Cited	MA3-914 was used in immunocytochemistry and western blot to study the distribution of calcium pumps in pancreatic and salivary gland cells J Biol Chem. 1997 Jun 20;272(25):15771-6. "Polarized expression of Ca2+ pumps in pancreatic and salivary gland cells. Role in initiation and propagation of [Ca2+]i waves." Author(s): Lee MG, Xu X, Zeng W, Diaz J, Kuo TH, Wuytack F, Racymaekers L, Muallem S Number of Citations: 25 (See PubMed article )

Immunohistochemistry
	Species / Dilution	Summary
	Am / 1:100	MA3-914 was used in immunohistochemistry to investigate how cytoplasmic free calcium in neurons from the salamander retina is regulated J Comp Neurol. 2004 Aug 2;475(4):463-80. "Expression of calcium transporters in the retina of the tiger salamander (Ambystoma tigrinum)." Author(s): Krizaj D, Liu X, Copenhagen DR Number of Citations: 1 (See PubMed article )
	Ca / 1:500 Ca / 1:200	MA3-914 was used in immunohistochemistry and western blot to examine the species of proteins in caveolae from canine airway smooth muscle Am J Physiol Lung Cell Mol Physiol. 2000 Dec;279(6):L1226-35. "Caveolae from canine airway smooth muscle contain the necessary components for a role in Ca(2+) handling." Author(s): Darby PJ, Kwan CY, Daniel EE Number of Citations: 8 (See PubMed article )
	Ca / 1:200	MA3-914 was used in immunohistochemistry to investigate the colocalization of nNOS with Cav-1 and calcium-handling proteins Am J Physiol Gastrointest Liver Physiol. 2001 Oct;281(4):G1101-14. "nNOS in canine lower esophageal sphincter: colocalized with Cav-1 and Ca2+-handling proteins?" Author(s): Daniel EE, Jury J, Wang YF Number of Citations: 4 (See PubMed article )
	Ck / Not Cited	MA3-914 was used in immunohistochemistry and western blot to investigate the distribution of PMCA ATPase and calbindin-D28 k in the laying hen oviduct Histochemistry. 1991;96(5):413-8. "Immunohistochemical localization of a calcium pump and calbindin-D28k in the oviduct of the laying hen." Author(s): Wasserman RH, Smith CA, Smith CM, Brindak ME, Fullmer CS, Krook L, Penniston JT, Kumar R Number of Citations: 2 (See PubMed article )
	Fe / 1:500	MA3-914 was used in immunohistochemistry to show the localization of PMCA in the cat placenta Placenta. 2003 May;24(5):453-61. "Localization of alkaline phosphatase and Ca2+-ATPase in the cat placenta." Author(s): Champion EE, Glazier JD, Greenwood SL, Mann SJ, Rawlings JM, Sibley CP, Jones CJ Number of Citations: 0 (See PubMed article )
	Hu / Not Cited	MA3-914 was used in immunohistochemistry and immunoprecipitation to investigate the distribution of a plasma membrane calcium-pump in choroid plexus Brain Res. 1989 Jun 12;489(2):355-60. "Cerebrospinal fluid calcium homeostasis: evidence for a plasma membrane Ca2+-pump in mammalian choroid plexus." Author(s): Borke JL, Caride AJ, Yaksh TL, Penniston JT, Kumar R Number of Citations: 4 (See PubMed article )
	Hu / 1:5,000	MA3-914 was used in western blot and immunohistochemistry to characterize the plasma membrane calcium ATPase isoform 1, 2, 3, 4 in human corneal epithelium. Mol Vis. 2005 Mar 2;11():169-78. "Expression and immunolocalization of plasma membrane calcium ATPase isoforms in human corneal epithelium." Author(s): Talarico EF Jr, Kennedy BG, Marfurt CF, Loeffler KU, Mangini NJ Number of Citations: 3 (See PubMed article )
	Rt / 1:1,000	MA3-914 was used in immunohistochemistry and western blot to investigate the distribution of PMCA ATPase in rat intestine Pflugers Arch. 1990 Sep;417(1):120-2. "Cellular and segmental distribution of Ca2(+)-pump epitopes in rat intestine." Author(s): Borke JL, Caride A, Verma AK, Penniston JT, Kumar R Number of Citations: 2 (See PubMed article )
	Rt / 1:20	MA3-914 was used in western blot, immunohistochemistry and immunoprecipitation to purify and characterize PMCA ATPase in rat liver plasma membranes J Biol Chem. 1990 Sep 15;265(26):16012-9. "Partial purification and characterization of the Ca2(+)-pumping ATPase of the liver plasma membrane." Author(s): Kessler F, Bennardini F, Bachs O, Serratosa J, James P, Caride AJ, Gazzotti P, Penniston JT, Carafoli E Number of Citations: 6 (See PubMed article )
	Rt / Not Cited	MA3-914 was used in immunohistochemistry and immunoprecipitation to investigate the distribution of a plasma membrane calcium-pump in choroid plexus Brain Res. 1989 Jun 12;489(2):355-60. "Cerebrospinal fluid calcium homeostasis: evidence for a plasma membrane Ca2+-pump in mammalian choroid plexus." Author(s): Borke JL, Caride AJ, Yaksh TL, Penniston JT, Kumar R Number of Citations: 4 (See PubMed article )
	Rt / 1:1,000	MA3-914 was used in blocking/activating experiment, immunohistochemistry and western blot to investigate the distribution of calcium ATPase and 28-kDa CaBP in rat kidney distal convoluted tubule principal cells Am J Physiol. 1989 Nov;257(5 Pt 2):F842-9. "Plasma membrane calcium pump and 28-kDa calcium binding protein in cells of rat kidney distal tubules." Author(s): Borke JL, Caride A, Verma AK, Penniston JT, Kumar R Number of Citations: 9 (See PubMed article )

Immunoprecipitation
	Species / Dilution	Summary
	Ca / Not Cited	MA3-914 was used in immunoprecipitation and immunocytochemistry to investigate the role of plasma-membrane calcium-ATPase during the mechanotransduction in sensory hair cells. J Neurosci. 2006 Jun 7;26(23):6172-80. "Splice-site A choice targets plasma-membrane Ca2+-ATPase isoform 2 to hair bundles." Author(s): Hill JK, Williams DE, LeMasurier M, Dumont RA, Strehler EE, Gillespie PG Number of Citations: 1 (See PubMed article )
	Hu / Not Cited	MA3-914 was used in immunoprecipitation and western blot to produce and characterize CHO cells with the overexpression of PMCA4CI isoform J Biol Chem. 1995 Jun 16;270(24):14643-50. "Isolation and characterization of a stable Chinese hamster ovary cell line overexpressing the plasma membrane Ca(2+)-ATPase." Author(s): Guerini D, Schröder S, Foletti D, Carafoli E Number of Citations: 3 (See PubMed article )
	Hu / 1:5,000	MA3-914 was used in immunoprecipitation and western blot to characterize a new plasma membrane calcium-ATPase isoform 2a in hair bundles . J Neurosci. 2001 Jul 15;21(14):5066-78. "Plasma membrane Ca2+-ATPase isoform 2a is the PMCA of hair bundles." Author(s): Dumont RA, Lins U, Filoteo AG, Penniston JT, Kachar B, Gillespie PG Number of Citations: 29 (See PubMed article )
	Ms / Not Cited	MA3-914 was used in immunocytochemistry, immunoprecipitation and western blot to investigate the expression of caclium-ATPase and NCX1 sodium/caclium exchanger in distal convoluted tubule cells Am J Physiol Renal Physiol. 2002 Jul;283(1):F29-40. "Plasma membrane Ca2+-ATPase and NCX1 Na+/Ca2+ exchanger expression in distal convoluted tubule cells." Author(s): Magyar CE, White KE, Rojas R, Apodaca G, Friedman PA Number of Citations: 1 (See PubMed article )
	Ms / Not Cited	MA3-914 was used in immunoprecipitation to investigate how cAMP regulates calcium clearance pathways in parotid acinar cells. J Biol Chem. 2002 Dec 13;277(50):48172-81. "Ca2+-dependent protein kinase--a modulation of the plasma membrane Ca2+-ATPase in parotid acinar cells." Author(s): Bruce JI, Yule DI, Shuttleworth TJ Number of Citations: 1 (See PubMed article )
	Rt / Not Cited	MA3-914 was used in western blot and immunoprecipitation to investigate the effect of phorbol ester on PMCA ATPase J Biol Chem. 1991 Feb 5;266(4):2520-5. "Phorbol ester induces both gene expression and phosphorylation of the plasma membrane Ca2+ pump." Author(s): Kuo TH, Wang KK, Carlock L, Diglio C, Tsang W Number of Citations: 2 (See PubMed article )
	Rt / Not Cited	MA3-914 was used in western blot, immunohistochemistry and immunoprecipitation to purify and characterize PMCA ATPase in rat liver plasma membranes J Biol Chem. 1990 Sep 15;265(26):16012-9. "Partial purification and characterization of the Ca2(+)-pumping ATPase of the liver plasma membrane." Author(s): Kessler F, Bennardini F, Bachs O, Serratosa J, James P, Caride AJ, Gazzotti P, Penniston JT, Carafoli E Number of Citations: 6 (See PubMed article )
	Rt / Not Cited	MA3-914 was used in immunohistochemistry and immunoprecipitation to investigate the distribution of a plasma membrane calcium-pump in choroid plexus Brain Res. 1989 Jun 12;489(2):355-60. "Cerebrospinal fluid calcium homeostasis: evidence for a plasma membrane Ca2+-pump in mammalian choroid plexus." Author(s): Borke JL, Caride AJ, Yaksh TL, Penniston JT, Kumar R Number of Citations: 4 (See PubMed article )
	Rt / Not Cited	MA3-914 was used in immunoprecipitation and western blot to study the interactions of HB-EGF, beta (1)-integrin, and CD9 in medullary thick ascending limbs Am J Physiol Cell Physiol. 2000 Jul;279(1):C136-46. "Osmotically relevant membrane signaling complex: association between HB-EGF, beta(1)-integrin, and CD9 in mTAL." Author(s): Sheikh-Hamad D, Youker K, Truong LD, Nielsen S, Entman ML Number of Citations: 1 (See PubMed article )
	Rt / 1:5,000	MA3-914 was used in immunoprecipitation and immunocytochemistry to investigate the role of plasma-membrane calcium-ATPase during the mechanotransduction in sensory hair cells. J Neurosci. 2006 Jun 7;26(23):6172-80. "Splice-site A choice targets plasma-membrane Ca2+-ATPase isoform 2 to hair bundles." Author(s): Hill JK, Williams DE, LeMasurier M, Dumont RA, Strehler EE, Gillespie PG Number of Citations: 1 (See PubMed article )

Western Blot
	Species / Dilution	Summary
	Am / Not Cited	MA3-914 was used in immunocytochemistry and western blot to investigate the mechanisms for plasma membrane calcium-ATPase (PMCA) internalization during oocyte maturation Dev Biol. 2008 Dec 1;324(1):99-107. "Internalization of plasma membrane Ca2+-ATPase during Xenopus oocyte maturation." Author(s): El-Jouni W, Haun S, Machaca K Number of Citations: 1 (See PubMed article )
	Bv / Not Cited	MA3-914 was used in western blot to study the effect of thapsigargin-sensitive calcium-ATPases on calcium homeostasis in adrenal chromaffin cells Biochem J. 1995 May 1;307 ( Pt 3)():749-58. "Thapsigargin-sensitive Ca(2+)-ATPases account for Ca2+ uptake to inositol 1,4,5-trisphosphate-sensitive and caffeine-sensitive Ca2+ stores in adrenal chromaffin cells." Author(s): Poulsen JC, Caspersen C, Mathiasen D, East JM, Tunwell RE, Lai FA, Maeda N, Mikoshiba K, Treiman M Number of Citations: 6 (See PubMed article )
	Ca / 1:1,000 Ca / 1:2,500	MA3-914 was used in immunohistochemistry and western blot to examine the species of proteins in caveolae from canine airway smooth muscle Am J Physiol Lung Cell Mol Physiol. 2000 Dec;279(6):L1226-35. "Caveolae from canine airway smooth muscle contain the necessary components for a role in Ca(2+) handling." Author(s): Darby PJ, Kwan CY, Daniel EE Number of Citations: 8 (See PubMed article )
	Ck / 1:500	MA3-914 was used in immunohistochemistry and western blot to investigate the distribution of PMCA ATPase and calbindin-D28 k in the laying hen oviduct Histochemistry. 1991;96(5):413-8. "Immunohistochemical localization of a calcium pump and calbindin-D28k in the oviduct of the laying hen." Author(s): Wasserman RH, Smith CA, Smith CM, Brindak ME, Fullmer CS, Krook L, Penniston JT, Kumar R Number of Citations: 2 (See PubMed article )
	Hm / 1:1,000	MA3-914 was used in western blot to investigate the role of the ubiquitination and proteasomal degradation during the thyrotropin-releasing hormone receptor processing. Mol Endocrinol. 2003 Sep;17(9):1777-91. "Thyrotropin-releasing hormone receptor processing: role of ubiquitination and proteasomal degradation." Author(s): Cook LB, Zhu CC, Hinkle PM Number of Citations: 1 (See PubMed article )
	Hu / 1:500	MA3-914 was used in western blot to investigate the human erythrocyte caclium pump through the use of mutants and monoclonal antibodies J Biol Chem. 1992 Jul 15;267(20):14244-9. "Use of expression mutants and monoclonal antibodies to map the erythrocyte Ca2+ pump." Author(s): Adamo HP, Caride AJ, Penniston JT Number of Citations: 7 (See PubMed article )
	Hu / Not Cited	MA3-914 was used in western blot and immunoprecipitation to investigate the effect of phorbol ester on PMCA ATPase J Biol Chem. 1991 Feb 5;266(4):2520-5. "Phorbol ester induces both gene expression and phosphorylation of the plasma membrane Ca2+ pump." Author(s): Kuo TH, Wang KK, Carlock L, Diglio C, Tsang W Number of Citations: 2 (See PubMed article )
	Hu / 1:300	MA3-914 was used in western blot, immunohistochemistry and immunoprecipitation to purify and characterize PMCA ATPase in rat liver plasma membranes J Biol Chem. 1990 Sep 15;265(26):16012-9. "Partial purification and characterization of the Ca2(+)-pumping ATPase of the liver plasma membrane." Author(s): Kessler F, Bennardini F, Bachs O, Serratosa J, James P, Caride AJ, Gazzotti P, Penniston JT, Carafoli E Number of Citations: 6 (See PubMed article )
	Hu / Not Cited	MA3-914 was used in western blot to investigate the distribution of plasma membrane calcium-ATPase and inositol 1,4,5-trisphosphate receptor in human platelet membranes Cell Calcium. 1995 Jan;17(1):65-70. "Distribution of plasma membrane Ca(2+)-ATPase and inositol 1,4,5-trisphosphate receptor in human platelet membranes." Author(s): Dean WL, Quinton TM Number of Citations: 2 (See PubMed article )
	Hu / Not Cited	MA3-914 was used in western blot to identify and locate calcium-ATPases in human platelet plasma and intracellular membranes Biochem J. 1995 Mar 15;306 ( Pt 3)():837-42. "Localization and identification of Ca2+ATPases in highly purified human platelet plasma and intracellular membranes. Evidence that the monoclonal antibody PL/IM 430 recognizes the SERCA 3 Ca2+ATPase in human platelets." Author(s): Bokkala S, el-Daher SS, Kakkar VV, Wuytack F, Authi KS Number of Citations: 3 (See PubMed article )
	Hu / Not Cited	MA3-914 was used in immunoprecipitation and western blot to produce and characterize CHO cells with the overexpression of PMCA4CI isoform J Biol Chem. 1995 Jun 16;270(24):14643-50. "Isolation and characterization of a stable Chinese hamster ovary cell line overexpressing the plasma membrane Ca(2+)-ATPase." Author(s): Guerini D, Schröder S, Foletti D, Carafoli E Number of Citations: 3 (See PubMed article )
	Hu / Not Cited	MA3-914 was used in western blot and ELISA to investigate the epitopes and isoform specificity of anti-hPMCA antibodies and to study hPMCA4's distribution and its alternative splicing variants in different human tissues Biochem J. 1996 May 15;316 ( Pt 1)():353-9. "Detection of isoform 4 of the plasma membrane calcium pump in human tissues by using isoform-specific monoclonal antibodies." Author(s): Caride AJ, Filoteo AG, Enyedi A, Verma AK, Penniston JT Number of Citations: 13 (See PubMed article )
	Hu / Not Cited	MA3-914 was used in western blot to study the tyrosine phosphorylation of PMCA in human platelets J Biol Chem. 1997 Jun 13;272(24):15113-9. "Regulation of platelet plasma membrane Ca2+-ATPase by cAMP-dependent and tyrosine phosphorylation." Author(s): Dean WL, Chen D, Brandt PC, Vanaman TC Number of Citations: 9 (See PubMed article )
	Hu / 1000	MA3-914 was used in immunocytochemistry and western blot to investigate the regulation of calcium pumping from hair cell stereocilia by PMCA J Neurosci. 1998 Jan 15;18(2):610-24. "Plasma membrane Ca2+-ATPase extrudes Ca2+ from hair cell stereocilia." Author(s): Yamoah EN, Lumpkin EA, Dumont RA, Smith PJ, Hudspeth AJ, Gillespie PG Number of Citations: 42 (See PubMed article )
	Hu / 1:1,000	MA3-914 was used in western blot to study the important roles of PMCA in the myocardium Circ Res. 1998 Nov 2;83(9):877-88. "Overexpression of the sarcolemmal calcium pump in the myocardium of transgenic rats." Author(s): Hammes A, Oberdorf-Maass S, Rother T, Nething K, Gollnick F, Linz KW, Meyer R, Hu K, Han H, Gaudron P, Ertl G, Hoffmann S, Ganten U, Vetter R, Schuh K, Benkwitz C, Zimmer HG, Neyses L Number of Citations: 8 (See PubMed article )
	Hu / 1:1,000	MA3-914 was used in western blot to study the role of the calciumATPases during nonmuscle cell calcium signaling Hypertension. 2000 Jan;35(1 Pt 1):91-102. "Platelet Ca(2+)ATPases : a plural, species-specific, and multiple hypertension-regulated expression system." Author(s): Martin V, Bredoux R, Corvazier E, Papp B, Enouf J Number of Citations: 5 (See PubMed article )
	Hu / 1:3,000	MA3-914 was used in western blot to study the possible effects of tyrosine phosphorylation of PMCA in hypertensive patients on inhibition of the calcium pump in hypertension Hypertension. 2000 Jan;35(1 Pt 1):103-7. "Tyrosine phosphorylation of human platelet plasma membrane Ca(2+)-ATPase in hypertension." Author(s): Blankenship KA, Dawson CB, Aronoff GR, Dean WL Number of Citations: 3 (See PubMed article )
	Hu / 1:500	MA3-914 was used in western blot to investigate the regulation of Ca2+-ATPase-mediated calcium extrusion by small GTPases and PI3K. J Biol Chem. 2000 Jun 30;275(26):19529-35. "Regulation of plasma membrane Ca2+-ATPase by small GTPases and phosphoinositides in human platelets." Author(s): Rosado JA, Sage SO Number of Citations: 1 (See PubMed article )
	Hu / 1:1,000	MA3-914 was used in western blot to study the association of plasma membrane calcium-ATPase with the cytoskeleton. J Biol Chem. 2001 May 4;276(18):14704-9. "Plasma membrane Ca(2+)-ATPase associates with the cytoskeleton in activated platelets through a PDZ-binding domain." Author(s): Zabe M, Dean WL Number of Citations: 1 (See PubMed article )
	Hu / Not Cited	MA3-914 was used in immunoprecipitation and western blot to characterize a new plasma membrane calcium-ATPase isoform 2a in hair bundles . J Neurosci. 2001 Jul 15;21(14):5066-78. "Plasma membrane Ca2+-ATPase isoform 2a is the PMCA of hair bundles." Author(s): Dumont RA, Lins U, Filoteo AG, Penniston JT, Kachar B, Gillespie PG Number of Citations: 29 (See PubMed article )
	Hu / Not Cited	MA3-914 was used in western blot to characterize three novel SERCA 3 isoforms from human or rat J Biol Chem. 2002 Jul 5;277(27):24442-52. "Three novel sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) 3 isoforms. Expression, regulation, and function of the membranes of the SERCA3 family." Author(s): Martin V, Bredoux R, Corvazier E, Van Gorp R, Kovacs T, Gelebart P, Enouf J Number of Citations: 1 (See PubMed article )
	Hu / 1:1,000	MA3-914 was used in western blot to investigate the role of the ubiquitination and proteasomal degradation during the thyrotropin-releasing hormone receptor processing. Mol Endocrinol. 2003 Sep;17(9):1777-91. "Thyrotropin-releasing hormone receptor processing: role of ubiquitination and proteasomal degradation." Author(s): Cook LB, Zhu CC, Hinkle PM Number of Citations: 1 (See PubMed article )
	Hu / Not Cited	MA3-914 was used in immunocytochemistry to investigate the important roles of action cytoskeleton in T-cell activation and its impact on calcium-NFAT signaling Mol Cell Biol. 2004 Feb;24(4):1628-39. "Actin cytoskeleton regulates calcium dynamics and NFAT nuclear duration." Author(s): Rivas FV, O'Keefe JP, Alegre ML, Gajewski TF Number of Citations: 11 (See PubMed article )
	Hu / 1:10,000	MA3-914 was used in western blot and immunohistochemistry to characterize the plasma membrane calcium ATPase isoform 1, 2, 3, 4 in human corneal epithelium. Mol Vis. 2005 Mar 2;11():169-78. "Expression and immunolocalization of plasma membrane calcium ATPase isoforms in human corneal epithelium." Author(s): Talarico EF Jr, Kennedy BG, Marfurt CF, Loeffler KU, Mangini NJ Number of Citations: 3 (See PubMed article )
	Hu / 1:2,000	MA3-914 was used in western blot to demonstrate the role of plasma membrane calcium ATPase in human breast cancer MCF-7 cell proliferation. J Biol Chem. 2005 Jul 22;280(29):27076-84. "Antisense-mediated Inhibition of the plasma membrane calcium-ATPase suppresses proliferation of MCF-7 cells." Author(s): Lee WJ, Robinson JA, Holman NA, McCall MN, Roberts-Thomson SJ, Monteith GR Number of Citations: 1 (See PubMed article )
	Ms / 1:1,000	MA3-914 was used in western blot to study the extrusion mechanism of the calcium efflux in proximal tubule cells J Gen Physiol. 1997 Feb;109(2):217-28. "Molecular dissection of Ca2+ efflux in immortalized proximal tubule cells." Author(s): White KE, Gesek FA, Nesbitt T, Drezner MK, Friedman PA Number of Citations: 1 (See PubMed article )
	Ms / 1:1,000	MA3-914 was used in immunocytochemistry, immunoprecipitation and western blot to investigate the expression of caclium-ATPase and NCX1 sodium/caclium exchanger in distal convoluted tubule cells Am J Physiol Renal Physiol. 2002 Jul;283(1):F29-40. "Plasma membrane Ca2+-ATPase and NCX1 Na+/Ca2+ exchanger expression in distal convoluted tubule cells." Author(s): Magyar CE, White KE, Rojas R, Apodaca G, Friedman PA Number of Citations: 1 (See PubMed article )
	Ms / 1:1,000	MA3-914 was used in western blot to investigate the functions of Homer proteins. J Cell Biol. 2003 Jul 21;162(2):293-303. "Homer 2 tunes G protein-coupled receptors stimulus intensity by regulating RGS proteins and PLCbeta GAP activities." Author(s): Shin DM, Dehoff M, Luo X, Kang SH, Tu J, Nayak SK, Ross EM, Worley PF, Muallem S Number of Citations: 1 (See PubMed article )
	Ms / Not Cited	MA3-914 was used in western blot to show the role of plasma membrane calcium ATPase in the principle piece of the sperm tail in hyperactivated sperm motility and male fertility. J Biol Chem. 2004 Aug 6;279(32):33742-50. "Targeted ablation of plasma membrane Ca2+-ATPase (PMCA) 1 and 4 indicates a major housekeeping function for PMCA1 and a critical role in hyperactivated sperm motility and male fertility for PMCA4." Author(s): Okunade GW, Miller ML, Pyne GJ, Sutliff RL, O'Connor KT, Neumann JC, Andringa A, Miller DA, Prasad V, Doetschman T, Paul RJ, Shull GE Number of Citations: 1 (See PubMed article )
	Ms / Not Cited	MA3-914 was used in western blot to investigate the role of PMCA during the aberrant localization of intracellular organelles caused by the lack of Mist1. J Biol Chem. 2005 Apr 1;280(13):12668-75. "Aberrant localization of intracellular organelles, Ca2+ signaling, and exocytosis in Mist1 null mice." Author(s): Luo X, Shin DM, Wang X, Konieczny SF, Muallem S Number of Citations: 1 (See PubMed article )
	Ms / Not Cited	MA3-914 was used in western blot to investigate the role of the potassium-dependent sodium/calcium-exchanger 2 in neuronal calcium control. J Biol Chem. 2006 Mar 10;281(10):6273-82. "Importance of K+-dependent Na+/Ca2+-exchanger 2, NCKX2, in motor learning and memory." Author(s): Li XF, Kiedrowski L, Tremblay F, Fernandez FR, Perizzolo M, Winkfein RJ, Turner RW, Bains JS, Rancourt DE, Lytton J Number of Citations: 1 (See PubMed article )
	Ms / Not Cited	MA3-914 was used in immunocytochemistry and western blot to investigate the effect of maitotoxin during the convertion of plasmalemmal calcium pump into a calcium-permeable nonselective cation channel Am J Physiol Cell Physiol. 2009 Dec;297(6):C1533-43. "Maitotoxin converts the plasmalemmal Ca(2+) pump into a Ca(2+)-permeable nonselective cation channel." Author(s): Sinkins WG, Estacion M, Prasad V, Goel M, Shull GE, Kunze DL, Schilling WP Number of Citations: 1 (See PubMed article )
	Ms / Not Cited	MA3-914 was used in western blot to examine the effects of sodium accumulation on diastolic function following Serca2 knockout J Physiol. 2010 Feb 1;588(Pt 3):465-78. "Sodium accumulation promotes diastolic dysfunction in end-stage heart failure following Serca2 knockout." Author(s): Louch WE, Hougen K, Mørk HK, Swift F, Aronsen JM, Sjaastad I, Reims HM, Roald B, Andersson KB, Christensen G, Sejersted OM Number of Citations: 1 (See PubMed article )
	Nhp / Not Cited	MA3-914 was used in western blot to detect the over-expression of plasma membrane calcium pump (PMCA) isoform 1a in RAEC J Biol Chem. 1996 Mar 8;271(10):5536-44. "Consequences of functional expression of the plasma membrane Ca2+ pump isoform 1a." Author(s): Liu BF, Xu X, Fridman R, Muallem S, Kuo TH Number of Citations: 8 (See PubMed article )
	Nhp / Not Cited	MA3-914 was used in western blot and ELISA to investigate the epitopes and isoform specificity of anti-hPMCA antibodies and to study hPMCA4's distribution and its alternative splicing variants in different human tissues Biochem J. 1996 May 15;316 ( Pt 1)():353-9. "Detection of isoform 4 of the plasma membrane calcium pump in human tissues by using isoform-specific monoclonal antibodies." Author(s): Caride AJ, Filoteo AG, Enyedi A, Verma AK, Penniston JT Number of Citations: 13 (See PubMed article )
	Nhp / 1:200	MA3-914 was used in western blot and ELISA to produce and characterize novel anti-PMCA antibodies and to investigate the expression and distribution of PMCA isoforms in rat brain J Biol Chem. 1997 Sep 19;272(38):23741-7. "Plasma membrane Ca2+ pump in rat brain. Patterns of alternative splices seen by isoform-specific antibodies." Author(s): Filoteo AG, Elwess NL, Enyedi A, Caride A, Aung HH, Penniston JT Number of Citations: 21 (See PubMed article )
	Rt / 1:500	MA3-914 was used in immunohistochemistry and western blot to investigate the distribution of PMCA ATPase and calbindin-D28 k in the laying hen oviduct Histochemistry. 1991;96(5):413-8. "Immunohistochemical localization of a calcium pump and calbindin-D28k in the oviduct of the laying hen." Author(s): Wasserman RH, Smith CA, Smith CM, Brindak ME, Fullmer CS, Krook L, Penniston JT, Kumar R Number of Citations: 2 (See PubMed article )
	Rt / 1:1,000	MA3-914 was used in immunohistochemistry and western blot to investigate the distribution of PMCA ATPase in rat intestine Pflugers Arch. 1990 Sep;417(1):120-2. "Cellular and segmental distribution of Ca2(+)-pump epitopes in rat intestine." Author(s): Borke JL, Caride A, Verma AK, Penniston JT, Kumar R Number of Citations: 2 (See PubMed article )
	Rt / Not Cited	MA3-914 was used in western blot and immunoprecipitation to investigate the effect of phorbol ester on PMCA ATPase J Biol Chem. 1991 Feb 5;266(4):2520-5. "Phorbol ester induces both gene expression and phosphorylation of the plasma membrane Ca2+ pump." Author(s): Kuo TH, Wang KK, Carlock L, Diglio C, Tsang W Number of Citations: 2 (See PubMed article )
	Rt / 1:300	MA3-914 was used in western blot, immunohistochemistry and immunoprecipitation to purify and characterize PMCA ATPase in rat liver plasma membranes J Biol Chem. 1990 Sep 15;265(26):16012-9. "Partial purification and characterization of the Ca2(+)-pumping ATPase of the liver plasma membrane." Author(s): Kessler F, Bennardini F, Bachs O, Serratosa J, James P, Caride AJ, Gazzotti P, Penniston JT, Carafoli E Number of Citations: 6 (See PubMed article )
	Rt / 1:1,000	MA3-914 was used in blocking/activating experiment, immunohistochemistry and western blot to investigate the distribution of calcium ATPase and 28-kDa CaBP in rat kidney distal convoluted tubule principal cells Am J Physiol. 1989 Nov;257(5 Pt 2):F842-9. "Plasma membrane calcium pump and 28-kDa calcium binding protein in cells of rat kidney distal tubules." Author(s): Borke JL, Caride A, Verma AK, Penniston JT, Kumar R Number of Citations: 9 (See PubMed article )
	Rt / Not Cited	MA3-914 was used in western blot to perform structural and functional analysis on purified caveolae from luminal plasmalemma of rat lung endothelium Proc Natl Acad Sci U S A. 1995 Feb 28;92(5):1759-63. "Caveolae from luminal plasmalemma of rat lung endothelium: microdomains enriched in caveolin, Ca(2+)-ATPase, and inositol trisphosphate receptor." Author(s): Schnitzer JE, Oh P, Jacobson BS, Dvorak AM Number of Citations: 25 (See PubMed article )
	Rt / Not Cited	MA3-914 was used in western blot to detect the over-expression of plasma membrane calcium pump (PMCA) isoform 1a in RAEC J Biol Chem. 1996 Mar 8;271(10):5536-44. "Consequences of functional expression of the plasma membrane Ca2+ pump isoform 1a." Author(s): Liu BF, Xu X, Fridman R, Muallem S, Kuo TH Number of Citations: 8 (See PubMed article )
	Rt / Not Cited	MA3-914 was used in western blot to detect the expression of the plasma membrane calcium-ATPase (PMCA) in myogenic cells J Biol Chem. 1996 Nov 29;271(48):30816-22. "Expression of the plasma membrane Ca2+-ATPase in myogenic cells." Author(s): Hammes A, Oberdorf-Maass S, Jenatschke S, Pelzer T, Maass A, Gollnick F, Meyer R, Afflerbach J, Neyses L Number of Citations: 2 (See PubMed article )
	Rt / Not Cited	MA3-914 was used in immunocytochemistry and western blot to study the distribution of calcium pumps in pancreatic and salivary gland cells J Biol Chem. 1997 Jun 20;272(25):15771-6. "Polarized expression of Ca2+ pumps in pancreatic and salivary gland cells. Role in initiation and propagation of [Ca2+]i waves." Author(s): Lee MG, Xu X, Zeng W, Diaz J, Kuo TH, Wuytack F, Racymaekers L, Muallem S Number of Citations: 25 (See PubMed article )
	Rt / 1:200	MA3-914 was used in western blot and ELISA to produce and characterize novel anti-PMCA antibodies and to investigate the expression and distribution of PMCA isoforms in rat brain J Biol Chem. 1997 Sep 19;272(38):23741-7. "Plasma membrane Ca2+ pump in rat brain. Patterns of alternative splices seen by isoform-specific antibodies." Author(s): Filoteo AG, Elwess NL, Enyedi A, Caride A, Aung HH, Penniston JT Number of Citations: 21 (See PubMed article )
	Rt / 1:1,000	MA3-914 was used in western blot to study the important roles of PMCA in the myocardium Circ Res. 1998 Nov 2;83(9):877-88. "Overexpression of the sarcolemmal calcium pump in the myocardium of transgenic rats." Author(s): Hammes A, Oberdorf-Maass S, Rother T, Nething K, Gollnick F, Linz KW, Meyer R, Hu K, Han H, Gaudron P, Ertl G, Hoffmann S, Ganten U, Vetter R, Schuh K, Benkwitz C, Zimmer HG, Neyses L Number of Citations: 8 (See PubMed article )
	Rt / 1:1,000	MA3-914 was used in western blot to study the role of the calciumATPases during nonmuscle cell calcium signaling Hypertension. 2000 Jan;35(1 Pt 1):91-102. "Platelet Ca(2+)ATPases : a plural, species-specific, and multiple hypertension-regulated expression system." Author(s): Martin V, Bredoux R, Corvazier E, Papp B, Enouf J Number of Citations: 5 (See PubMed article )
	Rt / 1:500	MA3-914 was used in immunoprecipitation and western blot to study the interactions of HB-EGF, beta (1)-integrin, and CD9 in medullary thick ascending limbs Am J Physiol Cell Physiol. 2000 Jul;279(1):C136-46. "Osmotically relevant membrane signaling complex: association between HB-EGF, beta(1)-integrin, and CD9 in mTAL." Author(s): Sheikh-Hamad D, Youker K, Truong LD, Nielsen S, Entman ML Number of Citations: 1 (See PubMed article )
	Rt / Not Cited	MA3-914 was used in western blot to locate cardiac sodium channels in caveolar membrane domains Circ Res. 2002 Mar 8;90(4):443-9. "Localization of cardiac sodium channels in caveolin-rich membrane domains: regulation of sodium current amplitude." Author(s): Yarbrough TL, Lu T, Lee HC, Shibata EF Number of Citations: 19 (See PubMed article )
	Rt / Not Cited	MA3-914 was used in western blot to characterize three novel SERCA 3 isoforms from human or rat J Biol Chem. 2002 Jul 5;277(27):24442-52. "Three novel sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) 3 isoforms. Expression, regulation, and function of the membranes of the SERCA3 family." Author(s): Martin V, Bredoux R, Corvazier E, Van Gorp R, Kovacs T, Gelebart P, Enouf J Number of Citations: 1 (See PubMed article )
	Rt / 1:1,000 Rt / 1:400	MA3-914 was used in western blot to study how the intracellular redox cycling agent paraquat affects the functions of PMCA in primary cortical neurons Free Radic Biol Med. 2009 Nov 15;47(10):1507-14. "Effects of paraquat-induced oxidative stress on the neuronal plasma membrane Ca(2+)-ATPase." Author(s): Zaidi A, Fernandes D, Bean JL, Michaelis ML Number of Citations: 1 (See PubMed article )

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