PKA alpha Antibody

PKA alpha Polyclonal Antibody for Western blot, IF, ICC, IHC (P)

>> See 8 other antibodies for PRKACA (PKACA, PKA C-alpha, cAMP-dependent protein kinase catalytic subunit alpha, protein kinase A catalytic subunit)
Synonyms:
PKACA, PKA C-alpha, cAMP-dependent protein kinase catalytic subunit alpha, protein kinase A catalytic subunit
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Mouse (Ms)
Immunogen: Recombinant fragment corresponding to a region within amino acids 111 and 351 of Human PKA alpha
Ordering Information
Pierce PKA alpha Antibody
Product #
PA5-28160
Size
100 ul
Price
$316.00
Purchase
Add PKA alpha Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:500-1:3000
Immunofluorescence (IF) 1:100-1:1000
Immunocytochemistry (ICC) 1:100-1:1000
Immunohistochemistry (Paraffin) (IHC (P)) 1:100-1:1000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 0.38mg/ml
Purification: Antigen affinity chromatography
Storage Buffer: 0.1M tris, pH 7, with 10% glycerol, 0.1M glycine
Preservative: 0.01% thimerosal
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA5-28160 targets PKA alpha in IF, IHC (P), and WB applications and shows reactivity with Human and mouse samples.

The PA5-28160 immunogen is recombinant fragment corresponding to a region within amino acids 111 and 351 of Human PKA alpha.
General Information
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Product Images
  • PKA alpha Antibody (PA5-28160) in WB
    PKA-alpha_PA5-28160_Antibody_WesternBlot.jpg

    Western Blot with anti-PKA alpha Polyclonal Antibody (PA5-28160) (PKA-alpha_PA5-28160_Antibody_WesternBlot.jpg)

    Western Blot with anti-PKA alpha Polyclonal Antibody (PA5-28160)

    Western Blot analysis of PKA alpha using anti-PKA alpha Polyclonal Antibody (PA5-28160), in Hela Cell Lysate (30 ug of cell lysate) and at a dilution of 1:1000.
  • PKA alpha Antibody (PA5-28160) in WB
    PKA-alpha_PA5-28160_Antibody_WesternBlot_2.jpg

    Western Blot with anti-PKA alpha Polyclonal Antibody (PA5-28160) (PKA-alpha_PA5-28160_Antibody_WesternBlot_2.jpg)

    Western Blot with anti-PKA alpha Polyclonal Antibody (PA5-28160)

    Western Blot analysis of PKA alpha using anti-PKA alpha Polyclonal Antibody (PA5-28160), in Mouse Brain (50 ug of cell lysate) and at a dilution of 1:1000.
  • PKA alpha Antibody (PA5-28160) in IF
    PKA-alpha_PA5-28160_Antibody_Immunofluorescence.jpg

    Immunofluorescence with anti-PKA alpha Polyclonal Antibody (PA5-28160) (PKA-alpha_PA5-28160_Antibody_Immunofluorescence.jpg)

    Immunofluorescence with anti-PKA alpha Polyclonal Antibody (PA5-28160)

    Immunofluorescence analysis of methanol-fixed HeLa cells using PKA alpha (PA5-28160) antibody at 1:200 dilution.
  • PKA alpha Antibody (PA5-28160) in IHC
    PKA-alpha_PA5-28160_Antibody_Immunohistochemistry.jpg

    Immunohistochemistry with anti-PKA alpha Polyclonal Antibody (PA5-28160) (PKA-alpha_PA5-28160_Antibody_Immunohistochemistry.jpg)

    Immunohistochemistry with anti-PKA alpha Polyclonal Antibody (PA5-28160)

    Immunohistochemical analysis of paraffin-embedded colon using PKA alpha (PA5-28160) antibody at 1:100 dilution.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific