* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Antigen affinity chromatography
0.1M tris, pH 7,
10% glycerol, 0.1M glycine
-20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA5-28160 targets PKA alpha in IF, IHC (P), and WB applications and shows reactivity with Human and mouse samples.
The PA5-28160 immunogen is recombinant fragment corresponding to a region within amino acids 111 and 351 of Human PKA alpha.
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
PKA alpha Antibody (PA5-28160) in WB PKA-alpha_PA5-28160_Antibody_WesternBlot.jpg
Western Blot with anti-PKA alpha Polyclonal Antibody (PA5-28160)