PIK3R1 Antibody (6G10)

PIK3R1 Monoclonal Antibody for Western blot, IF, ICC, IHC, FACS

>> See 12 other antibodies for PIK3R1
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: 6G10
Tested Species Reactivity: Human (Hu), Mouse (Ms)
Immunogen: Purified recombinant fragment of human PIK3R1 (amino acids: 159-388) expressed in E. Coli.
Ordering Information
Pierce PIK3R1 Antibody (6G10)
Product #
MA5-17150
Size
100 ug
Price
$310.00
Purchase
Add PIK3R1 Antibody (6G10) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:500 - 1:2000
Immunofluorescence (IF) 1:100
Immunocytochemistry (ICC) 1:100
Immunohistochemistry (IHC) 1:200 - 1:1000
Flow Cytometry (FACS) 1:200 - 1:400
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: purified
Storage Buffer: PBS with 0.5% proprietary stabilizer
Preservative: 0.05% sodium azide
Storage Conditions: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Specific Information
MA5-17150 targets PIK3R1 in FACS, ICC, IHC, IF and WB applications and shows reactivity with Human and Mouse samples.

The MA5-17150 immunogen is purified recombinant fragment of human PIK3R1 (amino acids: 159-388) expressed in E. Coli.

MA5-17150 detects PIK3R1 which has a predicted molecular weight of approximately 83.6kDa.
General Information
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms.
Product Images
  • PIK3R1 Antibody (MA5-17150) in WB
    PIK3R1_Antibody_MA5-17150_Westernblot1-1252013.jpg

    Western Blot with anti-PIK3R1 Monoclonal Antibody [6G10] (MA5-17150) (PIK3R1_Antibody_MA5-17150_Westernblot1-1252013.jpg)

    Western Blot with anti-PIK3R1 Monoclonal Antibody [6G10] (MA5-17150)

    Western blot analysis of PIK3R1 using a PIK3R1 monoclonal antibody (Product # MA5-17150) against a human PIK3R1 recombinant protein.
  • PIK3R1 Antibody (MA5-17150) in IF
    PIK3R1_Antibody_MA5-17150_Immunofluorescence1-1252013.jpg

    Immunofluorescence with anti-PIK3R1 Monoclonal Antibody [6G10] (MA5-17150) (PIK3R1_Antibody_MA5-17150_Immunofluorescence1-1252013.jpg)

    Immunofluorescence with anti-PIK3R1 Monoclonal Antibody [6G10] (MA5-17150)

    Immunofluorescence analysis of HeLa cells using PIK3R1 monoclonal antibody (Product # MA5-17150) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.
  • PIK3R1 Antibody (MA5-17150) in IF
    PIK3R1_Antibody_MA5-17150_Immunofluorescence2-1252013.jpg

    Immunofluorescence with anti-PIK3R1 Monoclonal Antibody [6G10] (MA5-17150) (PIK3R1_Antibody_MA5-17150_Immunofluorescence2-1252013.jpg)

    Immunofluorescence with anti-PIK3R1 Monoclonal Antibody [6G10] (MA5-17150)

    Immunofluorescence analysis of HepG2 cells using PIK3R1 monoclonal antibody (Product # MA5-17150) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.
  • PIK3R1 Antibody (MA5-17150) in IHC
    PIK3R1_Antibody_MA5-17150_Immunohistochemical1-1252013.jpg

    Immunohistochemistry with anti-PIK3R1 Monoclonal Antibody [6G10] (MA5-17150) (PIK3R1_Antibody_MA5-17150_Immunohistochemical1-1252013.jpg)

    Immunohistochemistry with anti-PIK3R1 Monoclonal Antibody [6G10] (MA5-17150)

    Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using PIK3R1 monoclonal antibody (Product # MA5-17150) followed with DAB staining.
  • PIK3R1 Antibody (MA5-17150) in IHC
    PIK3R1_Antibody_MA5-17150_Immunohistochemical2-1252013.jpg

    Immunohistochemistry with anti-PIK3R1 Monoclonal Antibody [6G10] (MA5-17150) (PIK3R1_Antibody_MA5-17150_Immunohistochemical2-1252013.jpg)

    Immunohistochemistry with anti-PIK3R1 Monoclonal Antibody [6G10] (MA5-17150)

    Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PIK3R1 monoclonal antibody (Product # MA5-17150) followed with DAB staining.
  • PIK3R1 Antibody (MA5-17150) in FACS
    PIK3R1_Antibody_MA5-17150_FlowCytometry-1252013.jpg

    Flow Cytometry with anti-PIK3R1 Monoclonal Antibody [6G10] (MA5-17150) (PIK3R1_Antibody_MA5-17150_FlowCytometry-1252013.jpg)

    Flow Cytometry with anti-PIK3R1 Monoclonal Antibody [6G10] (MA5-17150)

    Flow cytometric analysis of NIH3T3 cells using PIK3R1 monoclonal antibody (Product # MA5-17150) (green) and negative control (red).
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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