Immunofluorescent analysis of Hela cell stained with a PHOX2A polyclonal antibody (PA5-26387). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with a PHOX2A polyclonal antibody (PA5-26387) (1:25, 1 h at 37?). Primary antibody detected with fluor-conjugated donkey anti-rabbit secondary antibody (green) was used (1:400, 50 min at 37?). Cytoplasmic actin was counterstained with red dye conjugated Phalloidin (7units/ml, 1 h at 37?). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).
PHOX2A Antibody (PA5-26387) in IHC PHOX2A_Antibody_PA5-26387_IHC_1_112013.jpg
Immunohistochemistry with anti-PHOX2A Polyclonal Antibody (PA5-26387)
Immunohistochemical staining of formalin-fixed and paraffin-embedded human brain tissue using anti-PHOX2A Polyclonal Antibody (PA5-26387), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
PHOX2A Antibody (PA5-26387) in FACS PHOX2A_Antibody_PA5-26387_FC_1_112013.jpg
Flow Cytometry with anti-PHOX2A Polyclonal Antibody (PA5-26387)
Flow cytometric analysis of 293 cells using anti-PHOX2A Polyclonal Antibody (PA5-26387) (right) compared to a negative control cell (left) at a dilution of 1:10-50. Secondary antibody used to visualize was a Goat Anti-Rabbit Secondary Antibody Conjugated to FITC.
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