PHOX2A Antibody

(This product has been discontinued.)
Please click the link below to find suitable replacements for this discontinued product.

>> See 1 other antibodies for PHOX2A

PHOX2A Polyclonal Antibody for Western blot, IHC, FACS

>> See 2 other antibodies for PHOX2A (ARIX, CFEOM2, FEOM2, NCAM2, PMX2A, ARIX1 homeodomain protein, aristaless homeobox homolog, aristaless homeobox protein homolog, arix homeodomain protein, paired mesoderm homeobox protein 2A)
Synonyms:
ARIX, CFEOM2, FEOM2, NCAM2, PMX2A, ARIX1 homeodomain protein, aristaless homeobox homolog, aristaless homeobox protein homolog, arix homeodomain protein, paired mesoderm homeobox protein 2A
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu)
Immunogen: Synthetic peptide from the Center region of human PHOX2A conjugated to KLH.
Ordering Information
Pierce PHOX2A Antibody
Product #
PA5-26387
Size
400 ul
Price
$316.00
Purchase
(DISCONTINUED)
Tested Applications Dilution *
Western Blot (WB) 1:100-500
Immunohistochemistry (IHC) 1:50-100
Flow Cytometry (FACS) 1:10-50
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: Lot-specific
Purification: Antigen affinity chromatography
Storage Buffer: PBS
Preservative: 0.09% sodium azide
Storage Conditions: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Product Specific Information
PA5-26387 targets PHOX2A in FACS, IHC, and WB applications and shows reactivity with Human samples.

The PA5-26387 immunogen is synthetic peptide from the Center region of human PHOX2A conjugated to KLH.
General Information
Phox2a (paired-like homeobox 2a, aristaless homeobox homolog, Arix) is a transcription factor expressed in noradrenergic cell types of the sympathetic nervous systerm.
Product Images
  • PHOX2A Antibody (PA5-26387) in WB
    PHOX2A_Antibody_PA5-26387_WB_1_112013.jpg

    Western Blot with anti-PHOX2A Polyclonal Antibody (PA5-26387) (PHOX2A_Antibody_PA5-26387_WB_1_112013.jpg)

    Western Blot with anti-PHOX2A Polyclonal Antibody (PA5-26387)

    Western blot analysis of anti-PHOX2A Polyclonal Antibody (PA5-26387) in 293 cell line lysates (35 ug per lane).
  • PHOX2A Antibody (PA5-26387) in IF
    PHOX2A_Antibody_PA5-26387_IF_1_112013.jpg

    Immunofluorescence with anti-PHOX2A Polyclonal Antibody (PA5-26387) (PHOX2A_Antibody_PA5-26387_IF_1_112013.jpg)

    Immunofluorescence with anti-PHOX2A Polyclonal Antibody (PA5-26387)

    Immunofluorescent analysis of Hela cell stained with anti-PHOX2A Polyclonal Antibody (PA5-26387). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with anti-PHOX2A Polyclonal Antibody (PA5-26387) (1:25, 1 h at 37?). Primary antibody detected with Alexa Fluor® 488 conjugated donkey anti-rabbit secondary antibody (green) was used (1:400, 50 min at 37?). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37?). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).
  • PHOX2A Antibody (PA5-26387) in IHC
    PHOX2A_Antibody_PA5-26387_IHC_1_112013.jpg

    Immunohistochemistry with anti-PHOX2A Polyclonal Antibody (PA5-26387) (PHOX2A_Antibody_PA5-26387_IHC_1_112013.jpg)

    Immunohistochemistry with anti-PHOX2A Polyclonal Antibody (PA5-26387)

    Immunohistochemical staining of formalin-fixed and paraffin-embedded human brain tissue using anti-PHOX2A Polyclonal Antibody (PA5-26387), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
  • PHOX2A Antibody (PA5-26387) in FACS
    PHOX2A_Antibody_PA5-26387_FC_1_112013.jpg

    Flow Cytometry with anti-PHOX2A Polyclonal Antibody (PA5-26387) (PHOX2A_Antibody_PA5-26387_FC_1_112013.jpg)

    Flow Cytometry with anti-PHOX2A Polyclonal Antibody (PA5-26387)

    Flow cytometric analysis of 293 cells using anti-PHOX2A Polyclonal Antibody (PA5-26387) (right) compared to a negative control cell (left) at a dilution of 1:10-50. Secondary antibody used to visualize was a Goat Anti-Rabbit Secondary Antibody Conjugated to FITC.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific