PHLPP2 Antibody

PHLPP2 Polyclonal Antibody for Western blot, IHC, FACS

>> See 4 other antibodies for PHLPP2 (PHLPPL, PH domain leucine-rich repeat-containing protein phosphatase 2)
Synonyms:
PHLPPL, PH domain leucine-rich repeat-containing protein phosphatase 2
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu)
Immunogen: KLH conjugated synthetic peptide between 1295-1323 amino acids from the C-terminal region of human PHLPP2
Ordering Information
Pierce PHLPP2 Antibody
Product #
PA5-25995
Size
400 ul
Price
$316.00
Purchase
Add PHLPP2 Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:1000
Immunohistochemistry (IHC) 1:50-1:100
Flow Cytometry (FACS) 1:10-1:50
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: Lot-specific
Purification: Ammonium sulfate precipitation, Size-exclusion - Dialysis
Storage Buffer: PBS
Preservative: 0.09% sodium azide
Storage Conditions: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Product Specific Information
General Information
PHLPP2 is a protein phosphatase that specifically mediates dephosphorylation of 'Ser-473' of AKT1. This protein regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and decrease cell proliferation. It also controls the phosphorylation of AKT3.
Product Images
  • PHLPP2 Antibody (PA5-25995) in WB
    PHLPP2-_Antibody_PA5-25995_WB_1_112013.jpg

    Western Blot with anti-PHLPP2  Polyclonal Antibody (PA5-25995) (PHLPP2-_Antibody_PA5-25995_WB_1_112013.jpg)

    Western Blot with anti-PHLPP2 Polyclonal Antibody (PA5-25995)

    Western blot analysis using a PHLPP2 polyclonal antibody (Product # PA5-25995) in MDA-MB231 cell lysates (35ug per lane).
  • PHLPP2 Antibody (PA5-25995) in IHC
    PHLPP2-_Antibody_PA5-25995_IHC_1_112013.jpg

    Immunohistochemistry with anti-PHLPP2  Polyclonal Antibody (PA5-25995) (PHLPP2-_Antibody_PA5-25995_IHC_1_112013.jpg)

    Immunohistochemistry with anti-PHLPP2 Polyclonal Antibody (PA5-25995)

    Immunohistochemistry analysis in formalin-fixed, paraffin-embedded colon carcinoma using a PHLPP2 polyclonal antibody (Product # PA5-25995), followed by HRP-conjugated secondary antibody and DAB staining.
  • PHLPP2 Antibody (PA5-25995) in FACS
    PHLPP2-_Antibody_PA5-25995_FC_1_112013.jpg

    Flow Cytometry with anti-PHLPP2  Polyclonal Antibody (PA5-25995) (PHLPP2-_Antibody_PA5-25995_FC_1_112013.jpg)

    Flow Cytometry with anti-PHLPP2 Polyclonal Antibody (PA5-25995)

    Flow cytometry analysis of MDA-MB231 cells using a PHLPP2 polyclonal antibody (Product # PA5-25995) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific