PDX1 Antibody

PDX1 Polyclonal Antibody for Western blot

>> See 4 other antibodies for PDHX (Pyruvate dehydrogenase protein X component, Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex, E3-binding protein)
Synonyms:
Pyruvate dehydrogenase protein X component, Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex, E3-binding protein
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu ), Mouse (Ms)
Immunogen: Synthetic peptide from from the C-terminal region of PDX1 conjugated to KLH.
Ordering Information
Pierce PDX1 Antibody
Product #
PA5-11896
Size
400 ul
Price
$316.00
Purchase
Add PDX1 Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:100-1:500
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: Lot-specific
Purification: Protein G
Storage Buffer: PBS
Preservative: 0.09% sodium azide
Storage Conditions: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Product Specific Information
PA5-11896 targets PDX1 in WB applications and shows reactivity with Human and Mouse samples. The PA5-11896 immunogen is a synthetic peptide from from the C-terminal region of PDX1 conjugated to KLH. PA5-11896 detects PDX1 which has a predicted molecular weight of approximately 54 kDa.
General Information
The pyruvate dehydrogenase complex is located in the mitochondrial matrix and catalyzes the conversion of pyruvate to acetyl coenzyme A. The PDH complex thereby links glycolysis to Krebs cycle. The PDH complex contains three catalytic subunits, E1, E2, and E3, two regulatory subunits, E1 kinase and E1 phosphatase, and a non-catalytic subunit, E3 binding protein. This gene encodes the E3 binding protein subunit; also known as component X of the pyruvate dehydrogenase complex. This protein tethers E3 dimers to the E2 core of the PDH complex. Defects in this gene are a cause of pyruvate dehydrogenase deficiency which results in neurological dysfunction and lactic acidosis in infancy and early childhood. This protein is also a minor antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis. In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC eventually leads to cirrhosis and liver failure. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Product Images
  • PDX1 Antibody (PA5-11896) in WB
    PDX1_Antibody_PA5-11896_WB_3_112013.jpg

    Western Blot with anti-PDX1 Polyclonal Antibody (PA5-11896) (PDX1_Antibody_PA5-11896_WB_3_112013.jpg)

    Western Blot with anti-PDX1 Polyclonal Antibody (PA5-11896)

    Western blot analysis using anti-PDX1 Polyclonal Antibody (PA5-11896) in A2058 cell line lysates (35ug per lane).
  • PDX1 Antibody (PA5-11896) in WB
    PDX1_Antibody_PA5-11896_WB_2_112013.jpg

    Western Blot with anti-PDX1 Polyclonal Antibody (PA5-11896) (PDX1_Antibody_PA5-11896_WB_2_112013.jpg)

    Western Blot with anti-PDX1 Polyclonal Antibody (PA5-11896)

    Western blot analysis of PDX1 (arrow) using anti-PDX1 Polyclonal Antibody (PA5-11896). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDX1 gene (Lane 2).
  • PDX1 Antibody (PA5-11896) in WB
    PDX1_Antibody_PA5-11896_WB_1_112013.jpg

    Western Blot with anti-PDX1 Polyclonal Antibody (PA5-11896) (PDX1_Antibody_PA5-11896_WB_1_112013.jpg)

    Western Blot with anti-PDX1 Polyclonal Antibody (PA5-11896)

    Western blot analysis of PDX1 using anti-PDX1 Polyclonal Antibody (PA5-11896) in mouse heart tissue lysate.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific