PDX-1 Antibody

PDX-1 Polyclonal Antibody for Western blot, IF, IHC

>> See 3 other antibodies for PDX1 (Pyruvate Dehydrogenase Complex Component-X, PDHX, E3BP, DLDBP, Pyruvate Dehydrogenase Complex Lipoyl-Containing Component X)
Synonyms:
Pyruvate Dehydrogenase Complex Component-X, PDHX, E3BP, DLDBP, Pyruvate Dehydrogenase Complex Lipoyl-Containing Component X
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Mouse (Ms)
Published Species Reactivity: Human (Hu)
Immunogen: Synthetic Peptide: Q(21) R G P A P E F S A S P P A C(35)
Ordering Information
Pierce PDX-1 Antibody
Product #
PA3-830
Size
100 µl
Price
$360.00
Purchase
Add PDX-1 Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:3,000
Immunofluorescence (IF) 1:500
Immunohistochemistry (IHC) 1:500
Published Applications Dilution
Immunohistochemistry (IHC) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Storage Buffer: whole serum
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA3-830 detects human and mouse pdx-1 alpha and shows no cross-reactivity to other pdx isoforms.

PA3-830 has been successfully used in Western blot, immunohistochemical, and immunofluorescence procedures. By Western blot, this antibody detects an ~35 kDa protein.

PA3-830 immunogen is a KLH conjugated synthetic peptide corresponding to amino acid residues 21-35 from human pdx-1 protein. This antibody is generated against the N-terminal portion of the protein, while PA3-831 is C-terminal.

The PA3-830 antibody recognizes both cytoplasmic and nuclear proteins. Formalin-fixed tissues can cause high background, so counterstaining with another antibody which is mainly expressed in the cytoplasm (i.e. insulin) is recommended.
General Information
pdx-1 (pancreas duodenum homeobox-1, also known as IPF-1, IDX-1, and STF-1) is a transcription factor that has been shown to activate insulin, glucokinase, IAPP, and somatostatin gene transcription. Significant interest surrounds this proteins glucose-dependent regulation of insulin gene transcription.

Additionally, pdx-1 has been studied for its role in pancreatic development which includes maturation and differentiation of pancreatic precursor cells. Mutations in the pdx-1 gene have been shown to cause pancreatic agenesis, maturity-onset diabetes of the young, and type II diabetes.
Product Images
  • PDX-1 Antibody (PA3-830) in WB
    PA3-830_1_07232010.jpg

    Western Blot with anti-PDX-1 Polyclonal Antibody (PA3-830) (PA3-830_1_07232010.jpg)

    Western Blot with anti-PDX-1 Polyclonal Antibody (PA3-830)

    Western blot of pdx-1 in human pancreatic cancer cells MIA-1, PANC-1 and CAPAN-1 using PA3-830.
  • PDX-1 Antibody (PA3-830) in IF
    PDX1-Antibody-PA3-830-Immunofluorescence-IF.jpg

    Immunofluorescence with anti-PDX-1 Polyclonal Antibody (PA3-830) (PDX1-Antibody-PA3-830-Immunofluorescence-IF.jpg)

    Immunofluorescence with anti-PDX-1 Polyclonal Antibody (PA3-830)

    Immunofluorescent staining of pdx-1 in mouse islet cells using PA3-830 and counterstaining (merge) with insulin. The PA3-830 antibody recognizes both cytoplasmic and nuclear proteins. Formalin-fixed tissues can cause high background, so counterstaining with another antibody which is mainly expressed in the cytoplasm (i.e. insulin) is recommended.
  • PDX-1 Antibody (PA3-830) in IF
    PA3-830_IF_Hela.jpg

    Immunofluorescence with anti-PDX-1 Polyclonal Antibody (PA3-830) (PA3-830_IF_Hela.jpg)

    Immunofluorescence with anti-PDX-1 Polyclonal Antibody (PA3-830)

    Immunofluorescent analysis of PDX-1 in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a PDX-1 polyclonal antibody (Product # PA3-830) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). PDX-1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
  • PDX-1 Antibody (PA3-830) in IHC
    PA3-830_IHC_Human-Pancreas tissue.jpg

    Immunohistochemistry with anti-PDX-1 Polyclonal Antibody (PA3-830) (PA3-830_IHC_Human-Pancreas tissue.jpg)

    Immunohistochemistry with anti-PDX-1 Polyclonal Antibody (PA3-830)

    Immunohistochemistry was performed on normal biopsies of deparaffinized human pancreas tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a Rabbit Polyclonal Antibody recognizing PDX-1 (PA3-830 ) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  • PDX-1 Antibody (PA3-830) in IHC
    PA3-830_IHC_Human-Stomach tissue.jpg

    Immunohistochemistry with anti-PDX-1 Polyclonal Antibody (PA3-830) (PA3-830_IHC_Human-Stomach tissue.jpg)

    Immunohistochemistry with anti-PDX-1 Polyclonal Antibody (PA3-830)

    Immunohistochemistry was performed on normal biopsies of deparaffinized human stomach tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:500 with a Rabbit Polyclonal Antibody recognizing PDX-1 (PA3-830 ) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  • PDX-1 Antibody (PA3-830) in IHC
    PA3-830_IHC_Human-Tonsil tissue.jpg

    Immunohistochemistry with anti-PDX-1 Polyclonal Antibody (PA3-830) (PA3-830_IHC_Human-Tonsil tissue.jpg)

    Immunohistochemistry with anti-PDX-1 Polyclonal Antibody (PA3-830)

    Immunohistochemistry was performed on normal biopsies of deparaffinized human tonsil tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a Rabbit Polyclonal Antibody recognizing PDX-1 (PA3-830 ) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Publications:
Immunohistochemistry
  Species / Dilution Summary
  Hu / 1:100
PA3-830 was used in immunohistochemistry to investigate the expression of PDX-1 in human cancers through tissue microarray analyses

World J Surg. 2005 Mar;29(3):334-8.
"Tissue MicroArray analyses of pancreatic duodenal homeobox-1 in human cancers."
Author(s): Wang XP, Li ZJ, Magnusson J, Brunicardi FC
Number of Citations: 1
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific