PDGF Receptor beta / CD140b Antibody (G.290.3)

PDGF Receptor beta / CD140b Monoclonal Antibody for Western blot, IF, ICC, IHC (F), IHC (P), IP

>> See 31 other antibodies for PDGFRB (CD140 antigen-like family member B; PDGF-R-beta; beta-type platelet-derived growth factor receptor)
Synonyms:
CD140 antigen-like family member B; PDGF-R-beta; beta-type platelet-derived growth factor receptor
Entrez Gene ID:
Details
Host / Isotype: Rabbit / IgG
Class: Monoclonal
Type: Antibody
Clone: G.290.3
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt)
Published Species Reactivity: Rat (Rt)
Immunogen: GST fusion protein containing a carboxy-terminal fragment of human PDGF receptor ß
Ordering Information
Pierce PDGF Receptor beta / CD140b Antibody (G.290.3)
Product #
MA5-15143
Size
100 ul
Price
$250.00
Purchase
Add PDGF Receptor beta / CD140b Antibody (G.290.3) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:1000
Immunofluorescence (IF) 1:100
Immunocytochemistry (ICC) 1:100
Immunohistochemistry (Frozen) (IHC (F)) 1:100
Immunohistochemistry (Paraffin) (IHC (P)) 1:100
Immunoprecipitation (IP) 1:50
Published Applications Dilution
Immunocytochemistry (ICC) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Purification: Affinity chromatography
Storage Buffer: 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol
Preservative: <0.02% sodium azide
Storage Conditions: -20°C
Product Specific Information
It is not recommended to aliquot this antibody.
General Information
This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia.
Product Images
  • PDGF Receptor beta / CD140b Antibody (MA5-15143) in WB
    MA5-15143_WesternBlot.jpg

    Western Blot with anti-PDGF Receptor beta  /  CD140b Monoclonal Antibody [G.290.3] (MA5-15143) (MA5-15143_WesternBlot.jpg)

    Western Blot with anti-PDGF Receptor beta / CD140b Monoclonal Antibody [G.290.3] (MA5-15143)

    Western blot analysis of PDGF Receptor beta in extracts from NIH/3T3 and C6 cell lines using PDGF Receptor beta monoclonal antibody (Product # MA5-15143).
  • PDGF Receptor beta / CD140b Antibody (MA5-15143) in IF
    PDGFReceptorbeta_MA5-15143_Antibody_Immunofluorescence(IF)_2A.jpg

    Immunofluorescence with anti-PDGF Receptor beta  /  CD140b Monoclonal Antibody [G.290.3] (MA5-15143) (PDGFReceptorbeta_MA5-15143_Antibody_Immunofluorescence(IF)_2A.jpg)

    Immunofluorescence with anti-PDGF Receptor beta / CD140b Monoclonal Antibody [G.290.3] (MA5-15143)

  • PDGF Receptor beta / CD140b Antibody (MA5-15143) in IF
    MA5-15143_Immunofluorescence.jpg

    Immunofluorescence with anti-PDGF Receptor beta  /  CD140b Monoclonal Antibody [G.290.3] (MA5-15143) (MA5-15143_Immunofluorescence.jpg)

    Immunofluorescence with anti-PDGF Receptor beta / CD140b Monoclonal Antibody [G.290.3] (MA5-15143)

    Immunofluorescent analysis of PDGF Receptor beta in NIH/3T3 cells, PDGF-treated, using a PDGF Receptor beta monoclonal antibody (Product # MA5-15143) (green). DNA is labeled using a fluorescent blue dye.
  • PDGF Receptor beta / CD140b Antibody (MA5-15143) in IHC
    MA5-15143_Immunohistochemistry.jpg

    Immunohistochemistry with anti-PDGF Receptor beta  /  CD140b Monoclonal Antibody [G.290.3] (MA5-15143) (MA5-15143_Immunohistochemistry.jpg)

    Immunohistochemistry with anti-PDGF Receptor beta / CD140b Monoclonal Antibody [G.290.3] (MA5-15143)

    Immunohistochemical analysis of PDGF Receptor beta in paraffin-embedded human colon carcinoma using a PDGF Receptor beta monoclonal antibody (Product # MA5-15143).
Publications:
Immunocytochemistry
  Species / Dilution Summary
  Rt / Not Cited
MA5-15143 was used in immunocytochemistry to study the role of hepatic stellate cells as liver-resident mesenchymal stem cells and their role in hematopoiesis

Cell Physiol Biochem. 2013;31(2-3):290-304.
"Hepatic stellate cells support hematopoiesis and are liver-resident mesenchymal stem cells."
Author(s): Kordes C, Sawitza I, Götze S, Häussinger D
Number of Citations: 2
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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