PBK Antibody (2C8)

PBK Monoclonal Antibody for Western blot, IF, ICC, IHC, FACS

>> See 3 other antibodies for PBK
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG2b
Class: Monoclonal
Type: Antibody
Clone: 2C8
Tested Species Reactivity: Human (Hu)
Immunogen: Purified recombinant fragment of human PBK expressed in E. Coli.
Ordering Information
Pierce PBK Antibody (2C8)
Product #
MA5-17144
Size
100 ul
Price
$310.00
Purchase
Add PBK Antibody (2C8) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:500 - 1:2000
Immunofluorescence (IF) 1:200 - 1:1000
Immunocytochemistry (ICC) 1:200 - 1:1000
Immunohistochemistry (IHC) 1:200 - 1:1000
Flow Cytometry (FACS) 1:200 - 1:400
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Storage Buffer: ascites
Preservative: 0.03% sodium azide
Storage Conditions: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Specific Information
MA5-17144 targets PBK in FACS, ICC, IHC, IF and WB applications and shows reactivity with Human samples.

The MA5-17144 immunogen is purified recombinant fragment of human PBK expressed in E. Coli.

MA5-17144 detects PBK which has a predicted molecular weight of approximately 36kDa.
General Information
This genes encodes a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. This mitotic kinase may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis.
Product Images
  • PBK Antibody (MA5-17144) in WB
    PBK_Antibody_MA5-17144_Westernblot2-1252013.jpg

    Western Blot with anti-PBK Monoclonal Antibody [2C8] (MA5-17144) (PBK_Antibody_MA5-17144_Westernblot2-1252013.jpg)

    Western Blot with anti-PBK Monoclonal Antibody [2C8] (MA5-17144)

    Western blot analysis of PBK using PBK monoclonal antibody (Product # MA5-17144) in A431 (1) cell lysate.
  • PBK Antibody (MA5-17144) in WB
    PBK_Antibody_MA5-17144_Westernblot1-1252013.jpg

    Western Blot with anti-PBK Monoclonal Antibody [2C8] (MA5-17144) (PBK_Antibody_MA5-17144_Westernblot1-1252013.jpg)

    Western Blot with anti-PBK Monoclonal Antibody [2C8] (MA5-17144)

    Western blot analysis of PBK using a PBK monoclonal antibody (Product # MA5-17144) against a human PBK (AA: 50-230) recombinant protein.
  • PBK Antibody (MA5-17144) in IF
    PBK_Antibody_MA5-17144_Immunofluorescence1-1252013.jpg

    Immunofluorescence with anti-PBK Monoclonal Antibody [2C8] (MA5-17144) (PBK_Antibody_MA5-17144_Immunofluorescence1-1252013.jpg)

    Immunofluorescence with anti-PBK Monoclonal Antibody [2C8] (MA5-17144)

    Immunofluorescence analysis of HeLa cells using PBK monoclonal antibody (Product # MA5-17144) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.
  • PBK Antibody (MA5-17144) in IHC
    PBK_Antibody_MA5-17144_Immunohistochemical2-1252013.jpg

    Immunohistochemistry with anti-PBK Monoclonal Antibody [2C8] (MA5-17144) (PBK_Antibody_MA5-17144_Immunohistochemical2-1252013.jpg)

    Immunohistochemistry with anti-PBK Monoclonal Antibody [2C8] (MA5-17144)

    Immunohistochemical analysis of paraffin-embedded colon cancer tissues using PBK monoclonal antibody (Product # MA5-17144) followed with DAB staining.
  • PBK Antibody (MA5-17144) in IHC
    PBK_Antibody_MA5-17144_Immunohistochemical1-1252013.jpg

    Immunohistochemistry with anti-PBK Monoclonal Antibody [2C8] (MA5-17144) (PBK_Antibody_MA5-17144_Immunohistochemical1-1252013.jpg)

    Immunohistochemistry with anti-PBK Monoclonal Antibody [2C8] (MA5-17144)

    Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using PBK monoclonal antibody (Product # MA5-17144) followed with DAB staining.
  • PBK Antibody (MA5-17144) in FACS
    PBK_Antibody_MA5-17144_FlowCytometry-1252013.jpg

    Flow Cytometry with anti-PBK Monoclonal Antibody [2C8] (MA5-17144) (PBK_Antibody_MA5-17144_FlowCytometry-1252013.jpg)

    Flow Cytometry with anti-PBK Monoclonal Antibody [2C8] (MA5-17144)

    Flow cytometric analysis of HeLa cells using PBK monoclonal antibody (Product # MA5-17144) (green) and negative control (red).
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific