|Host / Isotype:||Mouse / IgG2b|
|Species Reactivity:||Human (Hu)|
|Immunogen:||Recombinant, GST tagged, PAS fusion protein expressed in E. coli.|
|Pierce PASKIN Antibody (mAB6)|
|Storage:||-20° C, Avoid Freeze/Thaw Cycles|
|Form:||100 µg of Protein A/G purified, in vitro produced IgG2a (1 mg/ml) in PBS with 1 mg/ml BSA and 0.05% sodium azide.|
|Western Blot (WB)||Assay dependent|
|Immunofluorescence (IF)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
|Product Specific Information|
|MA1-700 detects PASKIN from human samples.
MA1-700 has been successfully used in Western blot and immunofluorescence procedures.
The MA1-700 immunogen is recombinant, GST tagged, PAS fusion protein expressed in E. coli
This monoclonal antibody was expressed by hybridoma cells grown in vitro in ABR’s dialysis-based bioreactor system. All media, reagents, and other variables that affect the system are continuously monitored and controlled, ensuring a superior degree of precision and repeatability compared to an in vivo ascites production. Additionally, the serum free medium is further protein purified for improved antibody performance.
|PASKIN (PAS-Kinase) is thought to regulate protein synthesis in cells. It is a conserved gene product found in yeast as well as mammals. PASKIN contains two domains (PAS A and PAS B) as well as a serine/threonine kinase domain related to AMP kinases. PASKIN activity leads to decreased carbohydrate storage, as well as increased protein synthesis. PASKIN-dependent phosphorylation inhibits the activity of mammalian glycogen synthase.|
|Species / Dilution||Summary|
Hu / 1:50
MA1-700 was used in immunohistochemistry to study the PASKIN expression in male germ cell and its novel target eEF1A1
Cell Physiol Biochem. 2007;20(1-4):227-40.
"Male germ cell expression of the PAS domain kinase PASKIN and its novel target eukaryotic translation elongation factor eEF1A1."
Author(s): Eckhardt K, Troger J, Reissmann J, Katschinski DM, Wagner KF, Stengel P, Paasch U, Hunziker P, Borter E, Barth S, Schlafli P, Spielmann P, Stiehl DP, Camenisch G, Wenger RH
Number of Citations: 1
(See PubMed article )