* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
0.05% sodium azide
-20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA3-951 detects poly(ADP-ribose) polymerase (PARP) from mouse, rat, bovine and human tissues.
PA3-951 has been successfully used in Western blot and ELISA procedures. By Western blot, this antibody detects a 116 kDa protein representing PARP and the 85 kDa apoptosis-induced cleavage product of protease resembling ICE (prICE) and CPP32 from GH4C1 cell extract.
PA3-951 immunizing peptide corresponds to amino acid residues 509-524 from bovine PARP.
Poly(ADP-ribose) polymerase (PARP) is a eukaryotic nuclear protein involved in differentiation, DNA repair, and chromatin structure formation. During the process of programmed cell death, or apoptosis, the cell undergoes distinct morphological changes which include shrinkage, membrane blebbing, and nuclear reorganization. Several members of the interleukin 1-beta-converting enzyme (ICE)/ced-3 family of proteases, or caspases, are activated in a cascade of cleavage events which leads to the degradation of critical cellular substrates. PARP contains a conserved protease recognition site, DEVD (single letter code for amino acids), which is known to be a target for several caspases including prICE (protease resembling ICE) and CPP32/YAMA. Other nuclear events that are concurrent with PARP cleavage during apoptosis are activation of the domain nuclease and fragmentation nuclease which cleave DNA into >50 kb and nucleosome-sized fragments, respectively.