Oct4 / Pou5F1 Antibody (9B7)

Oct4 / Pou5F1 Monoclonal Antibody for Western blot, IF, FACS

>> See 11 other antibodies for POU5F1 (Oct-3; Oct-4; OCT3; OCT4; OTF-3; OTF3; OTF4; POU domain transcription factor OCT4, POU5F1, Homo sapiens POU class 5 homeobox 1 transcript variant 1, DADB-104B20.2, OTF-3, OTF3, OTF4, POU-type homeodomain-containing DNA-binding protein, octamer-binding protein 3, octamer-binding protein 4, octamer-binding transcription factor 3, octamer-binding transcription factor-3)
Synonyms:
Oct-3; Oct-4; OCT3; OCT4; OTF-3; OTF3; OTF4; POU domain transcription factor OCT4, POU5F1, Homo sapiens POU class 5 homeobox 1 transcript variant 1, DADB-104B20.2, OTF-3, OTF3, OTF4, POU-type homeodomain-containing DNA-binding protein, octamer-binding protein 3, octamer-binding protein 4, octamer-binding transcription factor 3, octamer-binding transcription factor-3
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG
Class: Monoclonal
Type: Antibody
Clone: 9B7
Tested Species Reactivity: Human (Hu)
Immunogen: Full length human recombinant protein of human POU5F1 produced in E.coli.
Ordering Information
Pierce Oct4 / Pou5F1 Antibody (9B7)
Product #
MA1-104
Size
100 µg
Price
$230.00
Purchase
Add Oct4 / Pou5F1 Antibody (9B7) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:500 - 1:5,000
Immunofluorescence (IF) 1:100 - 1:500
Flow Cytometry (FACS) 1:50 - 1:200
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Protein A
Storage Buffer: PBS with 1mg/ml BSA, 30% glycerol
Preservative: 0.05% sodium azide
Storage Conditions: -20°C
Product Specific Information
MA1-104 detects Oct4/Pou5F1 in Human embryonic carcinomic samples, but not in adult samples (e.g. HeLa cells). This antibody did not show reactivity in a mouse embryonic cell line (i.e. F9 embryonic carcinoma cells).

MA1-104 has been successfully used in FACS, IF, and WB. By Western blot, this antibody detects a band at 40 kDa in human NCCIT and NTERA-2 embryonal carcinoma cells, but not in negative control cells.
General Information
Oct4 is a transcription factor which plays a key role in embryonic development, especially during early embryogenesis. Oct4 is one of the four Yamaka factors, necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation.
Product Images
  • Oct4 / Pou5F1 Antibody (MA1-104) in WB
    Oct4_Antibody_MA1-104_Western-Blot.jpg

    Western Blot with anti-Oct4  /  Pou5F1 Monoclonal Antibody [9B7] (MA1-104) (Oct4_Antibody_MA1-104_Western-Blot.jpg)

    Western Blot with anti-Oct4 / Pou5F1 Monoclonal Antibody [9B7] (MA1-104)

    Western blot analysis of Oct4 was performed by loading 75 ug of the indicated whole cell lysates and 5ul of the Lane Marker Reducing Sample Buffer (Product # 39000) per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST (Product # 37525) for at least 1 hour. The membrane was probed with an Oct4 monoclonal antibody (Product # MA1-104) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgG-HRP secondary antibody (Product # 32430) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075). **Note: the antibody is detecting Oct4 in the human embryonal carcinoma cells, but not in negative control HeLa cells. Unknown bands at 80kDa were also observed with this antibody.
  • Oct4 / Pou5F1 Antibody (MA1-104) in IF
    Oct4_Antibody_MA1-104_Immunofluorescence_1.jpg

    Immunofluorescence with anti-Oct4  /  Pou5F1 Monoclonal Antibody [9B7] (MA1-104) (Oct4_Antibody_MA1-104_Immunofluorescence_1.jpg)

    Immunofluorescence with anti-Oct4 / Pou5F1 Monoclonal Antibody [9B7] (MA1-104)

    Immunofluorescent analysis of Oct4 (green) in retinoic acid-treated NCCIT cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 0.3% BSA/TBST (Product # 37525) for 15 minutes at room temperature. Cells were probed with an Oct4 monoclonal antibody (Product # MA1-104) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification. **Note: the absence of Oct4 staining in a subset of the NCCIT cells suggests differentiation. In addition to the nuclear staining of Oct4, the antibody also shows some cytoplasmic staining.
  • Oct4 / Pou5F1 Antibody (MA1-104) in FACS
    Oct4_Antibody_MA1-104_Flow-Cytometry.jpg

    Flow Cytometry with anti-Oct4  /  Pou5F1 Monoclonal Antibody [9B7] (MA1-104) (Oct4_Antibody_MA1-104_Flow-Cytometry.jpg)

    Flow Cytometry with anti-Oct4 / Pou5F1 Monoclonal Antibody [9B7] (MA1-104)

    Flow cytometry analysis of Oct4 (red histogram) on human NCCIT cells. Cells were harvested, fixed with 4% formaldehyde, washed with PBS, and incubated with an Oct4 monoclonal antibody (Product # MA1-104) at a 1:100 dilution or PBS alone (blue histogram) for 1 hour on ice. For flow analysis, a 30-minute incubation with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) was performed and 10,000 cells were acquired for each sample.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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