Oct-4A Antibody (T.631.9)

Oct-4A Monoclonal Antibody for Western blot, IF, ICC, FACS

>> See 11 other antibodies for POU5F1 (OTTHUMP00000221150; OTTHUMP00000221151; POU domain, class 5, transcription factor 1; POU-type homeodomain-containing DNA-binding protein; octamer-binding protein 3; octamer-binding protein 4; octamer-binding transcription factor 3; octamer-binding transcription factor-3)
Synonyms:
OTTHUMP00000221150; OTTHUMP00000221151; POU domain, class 5, transcription factor 1; POU-type homeodomain-containing DNA-binding protein; octamer-binding protein 3; octamer-binding protein 4; octamer-binding transcription factor 3; octamer-binding transcription factor-3
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit / IgG
Class: Monoclonal
Type: Antibody
Clone: T.631.9
Tested Species Reactivity: Human (Hu), Mouse (Ms)
Immunogen: Recombinant protein specific to the amino terminus of human Oct-4A
Ordering Information
Pierce Oct-4A Antibody (T.631.9)
Product #
MA5-14845
Size
100 ul
Price
$250.00
Purchase
Add Oct-4A Antibody (T.631.9) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:1000
Immunofluorescence (IF) 1:400
Immunocytochemistry (ICC) 1:400
Flow Cytometry (FACS) 1:600
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Purification: Affinity chromatography
Storage Buffer: 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol
Preservative: <0.02% sodium azide
Storage Conditions: -20°C
Product Specific Information
It is not recommended to aliquot this antibody.
General Information
This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.
Product Images
  • Oct-4A Antibody (MA5-14845) in IF
    MA5-14845_Immunofluorescence.jpg

    Immunofluorescence with anti-Oct-4A Monoclonal Antibody [T.631.9] (MA5-14845) (MA5-14845_Immunofluorescence.jpg)

    Immunofluorescence with anti-Oct-4A Monoclonal Antibody [T.631.9] (MA5-14845)

    Immunofluorescent analysis of Oct-4A in mouse embryonic stem cells growing on mouse embryonic fibroblast feeder cells, using an oct-4A monoclonal antibody (Product # MA5-14845) (green). Actin filaments are labeled with a fluorescent red phalloidin.
  • Oct-4A Antibody (MA5-14845) in IF
    MA5-14845_Immunofluorescence_2.jpg

    Immunofluorescence with anti-Oct-4A Monoclonal Antibody [T.631.9] (MA5-14845) (MA5-14845_Immunofluorescence_2.jpg)

    Immunofluorescence with anti-Oct-4A Monoclonal Antibody [T.631.9] (MA5-14845)

    Immunofluorescent analysis of Oct-4A in NTERA2 cells, using an oct-4A monoclonal antibody (Product # MA5-14845) (green). Actin filaments are labeled with a fluorescent red phalloidin.
  • Oct-4A Antibody (MA5-14845) in FACS
    MA5-14845_FlowCytometry.jpg

    Flow Cytometry with anti-Oct-4A Monoclonal Antibody [T.631.9] (MA5-14845) (MA5-14845_FlowCytometry.jpg)

    Flow Cytometry with anti-Oct-4A Monoclonal Antibody [T.631.9] (MA5-14845)

    Flow cytometric analysis of Oct-4A in HeLa cells (blue) and NCCIT cells (green) using a Oct-4A monoclonal antibody (Product # MA5-14845).
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific