OTX2 Antibody (1H12C4B5)

OTX2 Monoclonal Antibody for Western blot, IF, ICC, IHC, FACS

>> See 3 other antibodies for OTX2 (CPHD6, MCOPS5, homeobox protein OTX2; orthodenticle homolog 2)
Synonyms:
CPHD6, MCOPS5, homeobox protein OTX2; orthodenticle homolog 2
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: 1H12C4B5
Tested Species Reactivity: Human (Hu)
Immunogen: Purified recombinant fragment of human OTX2 expressed in E. Coli.
Ordering Information
Pierce OTX2 Antibody (1H12C4B5)
Product #
MA5-15854
Size
100 ul
Price
$316.00
Purchase
Add OTX2 Antibody (1H12C4B5) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1/500 - 1/2000
Immunofluorescence (IF) 1/200 - 1/1000
Immunocytochemistry (ICC) 1:200-1:1000
Immunohistochemistry (IHC) 1/200 - 1/1000
Flow Cytometry (FACS) 1/200 - 1/400
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Storage Buffer: ascites
Preservative: 0.03% sodium azide
Storage Conditions: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Specific Information
MA5-15854 targets OTX2 in FACS, IF, IHC, and WB applications and shows reactivity with Human samples.

The MA5-15854 immunogen is purified recombinant fragment of human OTX2 expressed in E. Coli. .

MA5-15854 detects OTX2 which has a predicted molecular weight of approximately 32kDa.
General Information
This gene encodes a member of the bicoid sub-family of homeodomain-containing transcription factors. The encoded protein acts as a transcription factor and may play a role in brain and sensory organ development. A similar protein in mice is required for proper forebrain development.Tissue specificity: Expressed in brain.
Product Images
  • OTX2 Antibody (MA5-15854) in WB
    OTX2_Antibody_MA5-15854_Westernblot1-1252013.jpg

    Western Blot with anti-OTX2 Monoclonal Antibody [1H12C4B5] (MA5-15854) (OTX2_Antibody_MA5-15854_Westernblot1-1252013.jpg)

    Western Blot with anti-OTX2 Monoclonal Antibody [1H12C4B5] (MA5-15854)

    Western blot analysis of OTX2 using a OTX2 monoclonal antibody (Product # MA5-15854) against a human OTX2 (AA: 40-297) recombinant protein.
  • OTX2 Antibody (MA5-15854) in WB
    OTX2_Antibody_MA5-15854_Westernblot2-1252013.jpg

    Western Blot with anti-OTX2 Monoclonal Antibody [1H12C4B5] (MA5-15854) (OTX2_Antibody_MA5-15854_Westernblot2-1252013.jpg)

    Western Blot with anti-OTX2 Monoclonal Antibody [1H12C4B5] (MA5-15854)

    Western blot analysis of OTX2 using OTX2 monoclonal antibody (Product # MA5-15854) in HepG2 (1), Jurkat (2), and NTERA-2 (3) cell lysate.
  • OTX2 Antibody (MA5-15854) in IF
    OTX2_Antibody_MA5-15854_Immunofluorescence1-1252013.jpg

    Immunofluorescence with anti-OTX2 Monoclonal Antibody [1H12C4B5] (MA5-15854) (OTX2_Antibody_MA5-15854_Immunofluorescence1-1252013.jpg)

    Immunofluorescence with anti-OTX2 Monoclonal Antibody [1H12C4B5] (MA5-15854)

    Immunofluorescence analysis of HepG2 cells using OTX2 monoclonal antibody (Product # MA5-15854) (Green). Red: actin filaments have been labeled with phalloidin.
  • OTX2 Antibody (MA5-15854) in IHC
    OTX2_Antibody_MA5-15854_Immunohistochemical1-1252013.jpg

    Immunohistochemistry with anti-OTX2 Monoclonal Antibody [1H12C4B5] (MA5-15854) (OTX2_Antibody_MA5-15854_Immunohistochemical1-1252013.jpg)

    Immunohistochemistry with anti-OTX2 Monoclonal Antibody [1H12C4B5] (MA5-15854)

    Immunohistochemical analysis of paraffin-embedded prostate tissues (left) and colon cancer tissues (right) using OTX2 monoclonal antibody (Product # MA5-15854) followed with DAB staining.
  • OTX2 Antibody (MA5-15854) in FACS
    OTX2_Antibody_MA5-15854_FlowCytometry-1252013.jpg

    Flow Cytometry with anti-OTX2 Monoclonal Antibody [1H12C4B5] (MA5-15854) (OTX2_Antibody_MA5-15854_FlowCytometry-1252013.jpg)

    Flow Cytometry with anti-OTX2 Monoclonal Antibody [1H12C4B5] (MA5-15854)

    Flow cytometric analysis of HepG2 cells using OTX2 monoclonal antibody (Product # MA5-15854) (green) and negative control (purple).
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific