* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Antigen affinity chromatography
PBS, pH 7,
-20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA5-28209 targets OCT1 in IF, IHC (P), and WB applications and shows reactivity with Human samples.
The PA5-28209 immunogen is recombinant protein fragment corresponding to a region within amino acids 442 and 755 of Human OCT1.
The OCT1 transcription factor was among the first identified members of the POU transcription factor family (summarized by Sturm et al., 1993 [PubMed 8314572]). Members of this family contain the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT.
OCT1 Antibody (PA5-28209) in WB PA5-28209_WB.jpg
Western Blot with anti-OCT1 Polyclonal Antibody (PA5-28209)
Western blot analysis of OCT1 using 30µg of A) 293T (B) A431 (C) HeLa and D) HepG2 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an OCT1 polyclonal antibody (Product # PA5-28209) at a dilution of 1:1000.
OCT1 Antibody (PA5-28209) in IF PA5-28209_IF.jpg
Immunofluorescence with anti-OCT1 Polyclonal Antibody (PA5-28209)
PA5-28209 immunoprecipitates OCT1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A: Control with 2.5 ?g of pre-immune rabbit IgG B: Immunoprecipitation of OCT1 by 2.5 ?g of OCT1 antibody (Product # PA5-28209) on 7.5% SDS-PAGE.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)