OATP8 Antibody (MDQ)

OATP8 Monoclonal Antibody for IF, IHC (F), IP

Synonyms:
Organic anion transporter polypeptide 8, Solute carrier organic anion transporter family member 1B3, Solute carrier family 21 member 8, Liver-specific
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: MDQ
Tested Species Reactivity: Human (Hu)
Published Species Reactivity: Human (Hu)
Immunogen: Synthetic peptide corresponding to residues M(1) D Q N Q H L N K T A E A Q P S E N K K T R Y(23) C of human OATP2.
Ordering Information
Pierce OATP8 Antibody (MDQ)
Product #
MA3-935
Size
500 µl
Price
$360.00
Purchase
Add OATP8 Antibody (MDQ) to your cart.
Tested Applications Dilution *
Immunofluorescence (IF) 1:20
Immunohistochemistry (Frozen) (IHC (F)) 1:20
Immunoprecipitation (IP) Assay dependent
Published Applications Dilution
Western Blot (WB) See publications below
Immunohistochemistry (IHC) See publications below
Immunoprecipitation (IP) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Storage Buffer: tissue culture supernatant
Preservative: 0.09% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
MA3-935 detects OATP8 from human tissue and cells. This antibody does not detect dog, mouse or rat protein. MA3-935 also detects the OATP2 isoform.

MA3-935 has been successfully used in immunohistochemistry, immunofluorescence and immunoprecipitation procedures. Immunofluorescence staining of OATP8 in human liver samples results in a pattern consistent with plasma membrane staining.

The MA3-935 immunogen is a synthetic peptide corresponding ot residues M(1) D Q N Q H L N K T A E A Q P S E N K K T R Y(23) C of human OATP2.
General Information
The OATPs (organic anion transporter polypeptides) are a family of transport proteins that have been found to mediate the uptake of organic ions into hepatocyte cells. These proteins fall into the solute carrier family 21A (SLC21A), and are expressed in liver, kidney, and brain. These polypeptides are involved in the excretion of potentially toxic compounds and therefore play a role in the overall detoxification system of the body. Unlike other members of the OATP family, OATP2 and 8 have been detected exclusively in the liver, and there is an 80% amino acid sequence homology between these polypeptides. Other names for OATP2 include SLC21A6, OATP-C, and LST-1.
Product Images
  • OATP8 Antibody (MA3-935) in IF
    MA3-935_IF_HepG2.jpg

    Immunofluorescence with anti-OATP8 Monoclonal Antibody [MDQ] (MA3-935) (MA3-935_IF_HepG2.jpg)

    Immunofluorescence with anti-OATP8 Monoclonal Antibody [MDQ] (MA3-935)

    Immunofluorescent analysis of OATP8 in HepG2 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a OATP8 monoclonal antibody (Product # MA3-935) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). OATP8 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
Publications:
Western Blot
  Species / Dilution Summary
  Hu / Not Cited
MA3-935 was used in immunohistochemistry, immunoprecipitation and western blot to detect organic anion transporters SLC21A6 and SLC21A8 in human liver and hepatocellular carcinoma

Lab Invest. 2003 Apr;83(4):527-38.
"Detection of the human organic anion transporters SLC21A6 (OATP2) and SLC21A8 (OATP8) in liver and hepatocellular carcinoma."
Author(s): Cui Y, König J, Nies AT, Pfannschmidt M, Hergt M, Franke WW, Alt W, Moll R, Keppler D
Number of Citations: 4
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Hu / Not Cited
MA3-935 was used in immunohistochemistry, immunoprecipitation and western blot to detect organic anion transporters SLC21A6 and SLC21A8 in human liver and hepatocellular carcinoma

Lab Invest. 2003 Apr;83(4):527-38.
"Detection of the human organic anion transporters SLC21A6 (OATP2) and SLC21A8 (OATP8) in liver and hepatocellular carcinoma."
Author(s): Cui Y, König J, Nies AT, Pfannschmidt M, Hergt M, Franke WW, Alt W, Moll R, Keppler D
Number of Citations: 4
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Hu / Not Cited
MA3-935 was used in immunohistochemistry, immunoprecipitation and western blot to detect organic anion transporters SLC21A6 and SLC21A8 in human liver and hepatocellular carcinoma

Lab Invest. 2003 Apr;83(4):527-38.
"Detection of the human organic anion transporters SLC21A6 (OATP2) and SLC21A8 (OATP8) in liver and hepatocellular carcinoma."
Author(s): Cui Y, König J, Nies AT, Pfannschmidt M, Hergt M, Franke WW, Alt W, Moll R, Keppler D
Number of Citations: 4
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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