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Nuclear Pore-O-Linked Glycoprotein Monoclonal Antibody (RL1)

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Nuclear Pore-O-Linked Glycoprotein Antibody for IF, IP, Western blot

>> See 6 other antibodies for Nup62 (Np62)

Synonyms:

Np62

Entrez Gene ID:

(Rat) 65274

UniProt ID:

P17955

Details

Host / Isotype:	Mouse / IgM
Class:	Monoclonal
Type:	Antibody
Clone:	RL1
Species Reactivity:	Rat (Rt) Amphibian (Am) Yeast (Ys)
Immunogen:	Pore complex-lamina fraction purified from rat liver nuclear envelopes.

Ordering Information
Pierce Nuclear Pore-O-Linked Glycoprotein Monoclonal Antibody (RL1)
Product Number	Pkg. Size	Price	Purchase

MA1-071	100 µl	$299.00	Qty:

Storage:	-20º C, Avoid Freeze/Thaw Cycles
Form:	100 µl of purified antibody in PBS containing 0.05% sodium azide.

Applications	Dilution *

Western Blot (WB)	1:1,000
Immunoprecipitation (IP)	Assay dependent
Immunofluorescence (IF)	1:100
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Product Specific Information

MA1-071 detects nuclear pore-O-linked glycoprotein from rat, amphibian and yeast.

MA1-071 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects up to eight different proteins from the nuclear pore complex (NPC) of approximately 210, 180, 145, 100, 63, 58, 54 and 45 kDa. Immunofluorescence staining of NPC O-linked glycoproteins with MA1-071 results in exclusive labeling of the NPC proteins on a wide variety of mammalian cells as well as S. cerevisiae and Xenopus. Labeling occurs exclusively at the NPC with most of the labeling at the cytoplasmic and nucleoplasmic margins.

Microinjected MA1-071 inhibits both protein import and RNA export in Xenopus oocytes.

The MA1-071 immunogen is pore complex-lamina fraction purified from rat liver nuclear envelopes.

General Information

Diffusion of metabolites and small non-nuclear molecules as well as active, mediated import of protein and export of protein and RNA through the nuclear envelope occurs through nuclear pore complexes or NPC’s. NPC’s contain up to 100 different polypeptides which have a combined mass of about 125 megadaltons. The channel available for passive transport through the NPC is about 9-10 nm in diameter while carrier mediated changes in the NPC result in a ~25 nm channel used for larger, actively transported molecules. Of the 100 polypeptides, at least 8 of these are O-linked N-acetylglycosamine-modified in mammalian cells. All of the mammalian O-linked glycoproteins contain multiple copies of phenylalanine, glycine dipeptide repeats dispersed throughout part of their sequence. Studies indicate that the NPC O-linked glycoproteins have a direct role in nuclear protein import.

PubMed References:

ICC (4)
IHC (4)
IP (3)
WB (8)

Immunocytochemistry
	Species / Dilution	Summary
	Hu / Not Cited	MA1-071 was used in immunocytochemistry to investigate the functional properties of lamin-binding fragment of LAP2 during the cell cycle and progression into S phase J Cell Biol. 1997 Dec 1;139(5):1077-87. "Lamin-binding fragment of LAP2 inhibits increase in nuclear volume during the cell cycle and progression into S phase." Author(s): Yang L, Guan T, Gerace L Number of Citations: 20 (See PubMed article )
	Rt / 5 ug/ml	MA1-071 was used in immunocytochemistry to investigate the role of gp210 in the assembly and functions of nuclear pore complex J Cell Biol. 1992 Jan;116(1):15-30. "Nuclear protein import is inhibited by an antibody to a lumenal epitope of a nuclear pore complex glycoprotein." Author(s): Greber UF, Gerace L Number of Citations: 21 (See PubMed article )
	Rt / 1:500	MA1-071 was used in immunocytochemistry and western blot to characterize a large coiled coil protein Tpr and investigate its localization J Cell Biol. 1994 Dec;127(6 Pt 1):1515-26. "Tpr, a large coiled coil protein whose amino terminus is involved in activation of oncogenic kinases, is localized to the cytoplasmic surface of the nuclear pore complex." Author(s): Byrd DA, Sweet DJ, Panté N, Konstantinov KN, Guan T, Saphire AC, Mitchell PJ, Cooper CS, Aebi U, Gerace L Number of Citations: 29 (See PubMed article )
	Ys / Not Cited	MA1-071 was used in immunocytochemistry to study how Rat7p regulates nucleocytoplasmic export of mRNA J Cell Biol. 1995 May;129(4):939-55. "A conditional allele of the novel repeat-containing yeast nucleoporin RAT7/NUP159 causes both rapid cessation of mRNA export and reversible clustering of nuclear pore complexes." Author(s): Gorsch LC, Dockendorff TC, Cole CN Number of Citations: 85 (See PubMed article )

Immunohistochemistry
	Species / Dilution	Summary
	Am / 17 ug/ml	MA1-071 was used in immunohistochemistry and western blot to investigate the important role of nuclear pore complex in transporting protein and RNA J Cell Biol. 1988 Oct;107(4):1289-97. "A monoclonal antibody against the nuclear pore complex inhibits nucleocytoplasmic transport of protein and RNA in vivo." Author(s): Featherstone C, Darby MK, Gerace L Number of Citations: 73 (See PubMed article )
	Bv / 10 ug/ml Ms / 10 ug/ml	MA1-071 was used in immunohistochemistry to investigate the distribution of RPGRIP1s and their association with RanBP2 in amacrine neurons Hum Mol Genet. 2003 Aug 1;12(15):1847-63. "RPGRIP1s with distinct neuronal localization and biochemical properties associate selectively with RanBP2 in amacrine neurons." Author(s): Castagnet P, Mavlyutov T, Cai Y, Zhong F, Ferreira P Number of Citations: 9 (See PubMed article )
	Rt / Not Cited	MA1-071 was used in immunohistochemistry, immunoprecipitation and western blot to identify novel glycoproteins from rat liver nuclear envelopes using home-made monoclonal antibodies J Cell Biol. 1987 May;104(5):1143-56. "Monoclonal antibodies identify a group of nuclear pore complex glycoproteins." Author(s): Snow CM, Senior A, Gerace L Number of Citations: 124 (See PubMed article )

Immunoprecipitation
	Species / Dilution	Summary
	Hu / 1:100	MA1-071 was used in immunoprecipitation to study the role of importin beta in nuclear export. J Cell Biol. 1999 May 17;145(4):645-57. "A role for RanBP1 in the release of CRM1 from the nuclear pore complex in a terminal step of nuclear export." Author(s): Kehlenbach RH, Dickmanns A, Kehlenbach A, Guan T, Gerace L Number of Citations: 42 (See PubMed article )
	Rt / 5 mg/ml	MA1-071 was used in immunohistochemistry, immunoprecipitation and western blot to identify novel glycoproteins from rat liver nuclear envelopes using home-made monoclonal antibodies J Cell Biol. 1987 May;104(5):1143-56. "Monoclonal antibodies identify a group of nuclear pore complex glycoproteins." Author(s): Snow CM, Senior A, Gerace L Number of Citations: 124 (See PubMed article )
	Rt / Not Cited	MA1-071 was used in immunoprecipitation and western blot to investigate the existence of O-linked GlcNAc residues in the cytoplasmic and nucleoplasmic proteins J Cell Biol. 1987 May;104(5):1157-64. "Nuclear pore complex glycoproteins contain cytoplasmically disposed O-linked N-acetylglucosamine." Author(s): Holt GD, Snow CM, Senior A, Haltiwanger RS, Gerace L, Hart GW Number of Citations: 65 (See PubMed article )

Western Blot
	Species / Dilution	Summary
	Am / Not Cited	MA1-071 was used in immunohistochemistry and western blot to investigate the important role of nuclear pore complex in transporting protein and RNA J Cell Biol. 1988 Oct;107(4):1289-97. "A monoclonal antibody against the nuclear pore complex inhibits nucleocytoplasmic transport of protein and RNA in vivo." Author(s): Featherstone C, Darby MK, Gerace L Number of Citations: 73 (See PubMed article )
	Hu / 1:500	MA1-071 was used in western blot to identify a new karyopherin exportin-5 and its cellular function. J Cell Biol. 2002 Jan 7;156(1):53-64. "Exportin-5, a novel karyopherin, mediates nuclear export of double-stranded RNA binding proteins." Author(s): Brownawell AM, Macara IG Number of Citations: 1 (See PubMed article )
	Inv / Not Cited	MA1-071 was used in western blot to study the effect of O-GlcNAcase knockout in C. elegans. Proc Natl Acad Sci U S A. 2006 Aug 8;103(32):11952-7. "Caenorhabditis elegans ortholog of a diabetes susceptibility locus: oga-1 (O-GlcNAcase) knockout impacts O-GlcNAc cycling, metabolism, and dauer." Author(s): Forsythe ME, Love DC, Lazarus BD, Kim EJ, Prinz WA, Ashwell G, Krause MW, Hanover JA Number of Citations: 1 (See PubMed article )
	Rt / Not Cited	MA1-071 was used in western blot to characterize a cytosolic factor indispensable for nuclear protein import J Cell Biol. 1992 Jan;116(2):271-80. "O-linked glycoproteins of the nuclear pore complex interact with a cytosolic factor required for nuclear protein import." Author(s): Sterne-Marr R, Blevitt JM, Gerace L Number of Citations: 26 (See PubMed article )
	Rt / 5 ug/ml	MA1-071 was used in immunohistochemistry, immunoprecipitation and western blot to identify novel glycoproteins from rat liver nuclear envelopes using home-made monoclonal antibodies J Cell Biol. 1987 May;104(5):1143-56. "Monoclonal antibodies identify a group of nuclear pore complex glycoproteins." Author(s): Snow CM, Senior A, Gerace L Number of Citations: 124 (See PubMed article )
	Rt / Not Cited	MA1-071 was used in immunohistochemistry and western blot to investigate the important role of nuclear pore complex in transporting protein and RNA J Cell Biol. 1988 Oct;107(4):1289-97. "A monoclonal antibody against the nuclear pore complex inhibits nucleocytoplasmic transport of protein and RNA in vivo." Author(s): Featherstone C, Darby MK, Gerace L Number of Citations: 73 (See PubMed article )
	Rt / Not Cited	MA1-071 was used in immunoprecipitation and western blot to investigate the existence of O-linked GlcNAc residues in the cytoplasmic and nucleoplasmic proteins J Cell Biol. 1987 May;104(5):1157-64. "Nuclear pore complex glycoproteins contain cytoplasmically disposed O-linked N-acetylglucosamine." Author(s): Holt GD, Snow CM, Senior A, Haltiwanger RS, Gerace L, Hart GW Number of Citations: 65 (See PubMed article )
	Rt / Not Cited	MA1-071 was used in immunocytochemistry and western blot to characterize a large coiled coil protein Tpr and investigate its localization J Cell Biol. 1994 Dec;127(6 Pt 1):1515-26. "Tpr, a large coiled coil protein whose amino terminus is involved in activation of oncogenic kinases, is localized to the cytoplasmic surface of the nuclear pore complex." Author(s): Byrd DA, Sweet DJ, Panté N, Konstantinov KN, Guan T, Saphire AC, Mitchell PJ, Cooper CS, Aebi U, Gerace L Number of Citations: 29 (See PubMed article )

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