NuMA Antibody

NuMA Polyclonal Antibody for Western blot, IF, ICC, IHC (P)

>> See 6 other antibodies for NUMA1 (NUMA, SP-H antigen, centrophilin stabilizes mitotic spindle in mitotic cells, structural nuclear protein)
Synonyms:
NUMA, SP-H antigen, centrophilin stabilizes mitotic spindle in mitotic cells, structural nuclear protein
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu)
Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 339 of NuMA (Uniprot ID#Q14980)
Ordering Information
Pierce NuMA Antibody
Product #
PA5-22285
Size
100 µL
Price
$324.00
Purchase
Tested Applications Dilution *
Western Blot (WB) 1:500-1:3000
Immunofluorescence (IF) 1:2000
Immunocytochemistry (ICC) 1:2000
Immunohistochemistry (Paraffin) (IHC (P)) 1:100-1:1000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 0.42mg/ml
Purification: Antigen affinity chromatography
Storage Buffer: 0.1M tris, pH 7, with 20% glycerol, 0.1M glycine
Preservative: 0.01% thimerosal
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA5-22285 targets NuMA in ICC, IF, IHC (P), and WB applications and shows reactivity with Human samples.

The PA5-22285 immunogen is a recombinant fragment corresponding to a region within amino acids 1 and 339 of NuMA (Uniprot ID#Q14980).
General Information
Product Images
  • NuMA Antibody (PA5-22285) in WB
    PA5-22285_WB.jpg

    Western Blot with anti-NuMA Polyclonal Antibody (PA5-22285) (PA5-22285_WB.jpg)

    Western Blot with anti-NuMA Polyclonal Antibody (PA5-22285)

    Western blot analysis of NuMA using A) 30 µg HeLa whole cell lysate and B) 30 µg A375 whole cell lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a NuMA polyclonal antibody (Product # PA5-22285) at a dilution of 1:1000.
  • NuMA Antibody (PA5-22285) in IF
    PA5-22285_IF.jpg

    Immunofluorescence with anti-NuMA Polyclonal Antibody (PA5-22285) (PA5-22285_IF.jpg)

    Immunofluorescence with anti-NuMA Polyclonal Antibody (PA5-22285)

    Immunofluorescent analysis of NuMA in paraformaldehyde-fixed U2OS cells using a NuMA polyclonal antibody (Product # PA5-22285) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
  • NuMA Antibody (PA5-22285) in IHC (P)
    PA5-22285_IHC.jpg

    Immunohistochemistry (Paraffin) with anti-NuMA Polyclonal Antibody (PA5-22285) (PA5-22285_IHC.jpg)

    Immunohistochemistry (Paraffin) with anti-NuMA Polyclonal Antibody (PA5-22285)

    Immunohistochemical analysis of NuMA in paraffin-embedded Cal27 xenograft using a NuMA polyclonal antibody (Product # PA5-22285) at a dilution of 1:100.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific