Notch-1 Antibody (A6)

Notch-1 Monoclonal Antibody for Western blot, IF, ICC, FACS

>> See 7 other antibodies for NOTCH1 (Notch homolog 1, translocation-associated, neurogenic locus notch homolog protein 1, translocation-associated notch protein TAN-1)
Synonyms:
Notch homolog 1, translocation-associated, neurogenic locus notch homolog protein 1, translocation-associated notch protein TAN-1
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG2b
Class: Monoclonal
Type: Antibody
Clone: A6
Tested Species Reactivity: Human (Hu), Mouse (Ms)
Published Species Reactivity: Human (Hu), Mouse (Ms)
Immunogen: Recombinant protein encoding the ligand binding region of human Notch-1
Ordering Information
Pierce Notch-1 Antibody (A6)
Product #
MA5-11961
Size
500 ul
Price
$316.00
Purchase
Add Notch-1 Antibody (A6) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:100-1:1000
Immunofluorescence (IF) 1:20-1:200
Immunocytochemistry (ICC) 1:20-1:200
Flow Cytometry (FACS) Assay Dependent
Published Applications Dilution
Western Blot (WB) See publications below
Immunocytochemistry (ICC) See publications below
Immunohistochemistry (IHC) See publications below
Flow Cytometry (FACS) See publications below
ELISA (ELISA) See publications below
Blocking Assay (BLOCK) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 0.2mg/ml
Purification: Protein A
Storage Buffer: PBS, pH 7.4, with 0.2% BSA
Preservative: 0.09% sodium azide
Storage Conditions: 4° C
Product Specific Information
MA5-11961 targets Notch-1 in WB, FACS and ICC/IF applications and shows reactivity with Human and mouse samples.

The MA5-11961 immunogen is recombinant protein encoding the ligand binding region of human Notch-1.
General Information
The notch gene belongs to a family of epidermal growth factor (EGF) like homeotic genes, which encode transmembrane proteins with a variable number of cysteine-rich EGF-like repeats in the extracellular region. Four notch genes have been described in mammals, which include notch-1. notch-2, notch-3, and notch-4(Int-3), which have been implicated in the differentiation of the nervous system and other structures. The EGF-like proteins delta and Serrate have been identified as ligands of notch-1. Mature notch proteins are heterodimeric receptors derived from the cleavage of notch pre-proteins into an extracellular subunit (NEC) containing multiple EGF-like repeats and a transmembrane subunit including intracellular region (Ntm). Alteration of notch-1 signaling or expression may contribute to tumorigenesis.
Product Images
  • Notch-1 Antibody (MA5-11961) in WB
    MA5-11961_WB_20131010123628.jpg

    Western Blot with anti-Notch-1 Monoclonal Antibody [A6] (MA5-11961) (MA5-11961_WB_20131010123628.jpg)

    Western Blot with anti-Notch-1 Monoclonal Antibody [A6] (MA5-11961)

    Western blot analysis of Notch-1 was performed by loading 25 ug of Hela cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a Notch-1 monoclonal antibody (Product # MA5-11961) at a dilution of 1:200 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~273kDa.
  • Notch-1 Antibody (MA5-11961) in IF
    MA5-11961_IF_Hela_20130724073842.jpg

    Immunofluorescence with anti-Notch-1 Monoclonal Antibody [A6] (MA5-11961) (MA5-11961_IF_Hela_20130724073842.jpg)

    Immunofluorescence with anti-Notch-1 Monoclonal Antibody [A6] (MA5-11961)

    Immunofluorescent analysis of Notch-1 (green) showing staining in the membrane of HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Notch-1 monoclonal antibody (Product # MA5-11961) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 100x.
  • Notch-1 Antibody (MA5-11961) in IF
    MA5-11961_IF_MCF-7_20130724073902.jpg

    Immunofluorescence with anti-Notch-1 Monoclonal Antibody [A6] (MA5-11961) (MA5-11961_IF_MCF-7_20130724073902.jpg)

    Immunofluorescence with anti-Notch-1 Monoclonal Antibody [A6] (MA5-11961)

    Immunofluorescent analysis of Notch-1 (green) showing staining in the membrane of MCF-7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Notch-1 monoclonal antibody (Product # MA5-11961) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 100x.
  • Notch-1 Antibody (MA5-11961) in IF
    MA5-11961_IF_NIH-3T3_20130724073924.jpg

    Immunofluorescence with anti-Notch-1 Monoclonal Antibody [A6] (MA5-11961) (MA5-11961_IF_NIH-3T3_20130724073924.jpg)

    Immunofluorescence with anti-Notch-1 Monoclonal Antibody [A6] (MA5-11961)

    Immunofluorescent analysis of Notch-1 (green) showing staining in the membrane of NIH-3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Notch-1 monoclonal antibody (Product # MA5-11961) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 100x.
  • Notch-1 Antibody (MA5-11961) in FACS
    MA5-11961_FACS_Jurkat.jpg

    Flow Cytometry with anti-Notch-1 Monoclonal Antibody [A6] (MA5-11961) (MA5-11961_FACS_Jurkat.jpg)

    Flow Cytometry with anti-Notch-1 Monoclonal Antibody [A6] (MA5-11961)

    Flow cytometry analysis of Notch-1 in Jurkat cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a Notch-1 monoclonal antibody (Product # MA5-11961) at a dilution of 1 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.
  • Notch-1 Antibody (MA5-11961) in FACS
    MA5-11961_FACS_NIH-3T3.jpg

    Flow Cytometry with anti-Notch-1 Monoclonal Antibody [A6] (MA5-11961) (MA5-11961_FACS_NIH-3T3.jpg)

    Flow Cytometry with anti-Notch-1 Monoclonal Antibody [A6] (MA5-11961)

    Flow cytometry analysis of Notch-1 in NIH-3T3 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a Notch-1 monoclonal antibody (Product # MA5-11961) at a dilution of 0.5 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.
Publications:
Western Blot
  Species / Dilution Summary
  Hu / 1:100
MA5-11961 was used in immunohistochemistry and western blot to investigate the role of Notch-1 and its ligands in glioma cell survival and proliferation

Cancer Res. 2005 Mar 15;65(6):2353-63.
"Expression of Notch-1 and its ligands, Delta-like-1 and Jagged-1, is critical for glioma cell survival and proliferation."
Author(s): Purow BW, Haque RM, Noel MW, Su Q, Burdick MJ, Lee J, Sundaresan T, Pastorino S, Park JK, Mikolaenko I, Maric D, Eberhart CG, Fine HA
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
MA5-11961 was used in western blot to study the involvement of NB-3/Notch1 pathway in the development of oligodendrocytes

J Biol Chem. 2004 Jun 11;279(24):25858-65.
"NB-3/Notch1 pathway via Deltex1 promotes neural progenitor cell differentiation into oligodendrocytes."
Author(s): Cui XY, Hu QD, Tekaya M, Shimoda Y, Ang BT, Nie DY, Sun L, Hu WP, Karsak M, Duka T, Takeda Y, Ou LY, Dawe GS, Yu FG, Ahmed S, Jin LH, Schachner M, Watanabe K, Arsenijevic Y, Xiao ZC
Number of Citations: 20
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Hu / 0
MA5-11961 was used in ELISA and immunocytochemistry to study the role of Notch pathway in T cell allorecognition

J Immunol. 2007 Jul 15;179(2):819-29.
"Antiparallel segregation of notch components in the immunological synapse directs reciprocal signaling in allogeneic Th:DC conjugates."
Author(s): Luty WH, Rodeberg D, Parness J, Vyas YM
Number of Citations: 3
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Hu / 1:200
MA5-11961 was used in immunohistochemistry and western blot to investigate the role of Notch-1 and its ligands in glioma cell survival and proliferation

Cancer Res. 2005 Mar 15;65(6):2353-63.
"Expression of Notch-1 and its ligands, Delta-like-1 and Jagged-1, is critical for glioma cell survival and proliferation."
Author(s): Purow BW, Haque RM, Noel MW, Su Q, Burdick MJ, Lee J, Sundaresan T, Pastorino S, Park JK, Mikolaenko I, Maric D, Eberhart CG, Fine HA
Number of Citations: 1
(See PubMed article )
  Hu / 1:100
MA5-11961 was used in immunohistochemistry to study the role of the Notch pathway in pancreatic cancer angiogenesis and neuroinvasion

Ann Surg. 2005 Dec;242(6):791-800, discussion 800-1.
"The Notch signaling pathway is related to neurovascular progression of pancreatic cancer."
Author(s): Büchler P, Gazdhar A, Schubert M, Giese N, Reber HA, Hines OJ, Giese T, Ceyhan GO, Müller M, Büchler MW, Friess H
Number of Citations: 16
(See PubMed article )
  Hu / 0
MA5-11961 was used in immunohistochemistry to study the histogenetic heterogeneity of sarcomatoid carcinomas of the lung

Virchows Arch. 2006 Oct;449(4):455-61.
"Sarcomatoid carcinomas of the lung--are these histogenetically heterogeneous tumors?"
Author(s): Blaukovitsch M, Halbwedl I, Kothmaier H, Gogg-Kammerer M, Popper HH
Number of Citations: 5
(See PubMed article )
  Hu / 10-20 ug/ml
MA5-11961 was used in immunohistochemistry to investigate the expression of stem cell markers in normal and diseased buccal mucosa

J Oral Sci. 2007 Jun;49(2):133-9.
"Changes in the expression of stem cell markers in oral lichen planus and hyperkeratotic lesions."
Author(s): Köse O, Lalli A, Kutulola AO, Odell EW, Waseem A
Number of Citations: 0
(See PubMed article )
Flow Cytometry
  Species / Dilution Summary
  Hu / 1:100
MA5-11961 was used in flow cytometry to identify genetic alterations in childhood ependymomas

J Clin Oncol. 2009 Apr 10;27(11):1884-92.
"Candidate genes on chromosome 9q33-34 involved in the progression of childhood ependymomas."
Author(s): Puget S, Grill J, Valent A, Bieche I, Dantas-Barbosa C, Kauffmann A, Dessen P, Lacroix L, Geoerger B, Job B, Dirven C, Varlet P, Peyre M, Dirks PB, Sainte-Rose C, Vassal G
Number of Citations: 7
(See PubMed article )
ELISA
  Species / Dilution Summary
  Hu / 0
MA5-11961 was used in ELISA and immunocytochemistry to study the role of Notch pathway in T cell allorecognition

J Immunol. 2007 Jul 15;179(2):819-29.
"Antiparallel segregation of notch components in the immunological synapse directs reciprocal signaling in allogeneic Th:DC conjugates."
Author(s): Luty WH, Rodeberg D, Parness J, Vyas YM
Number of Citations: 3
(See PubMed article )
Blocking Assay
  Species / Dilution Summary
  Hu / 20 ug/ml
MA5-11961 was used in blocking or activating experiment to investigate the influence of blockage of Notch1 signaling on T-helper cell balance in patients with chronic hepatitis B viral infection

Hepatol Res. 2010 Aug;40(8):799-805.
"Blockage of Notch1 signaling modulates the T-helper (Th)1/Th2 cell balance in chronic hepatitis B patients."
Author(s): Pei J, Tang Z, Zang G, Yu Y
Number of Citations: 1
(See PubMed article )
  Hu / 10 ug/ml
MA5-11961 was used in blocking or activating experiment to study the mechanism by which Notch1 regulates regulatory T-cell induction by mesenchymal stem cells

Eur J Immunol. 2013 Jan;43(1):182-7.
"Notch1 modulates mesenchymal stem cells mediated regulatory T-cell induction."
Author(s): Del Papa B, Sportoletti P, Cecchini D, Rosati E, Balucani C, Baldoni S, Fettucciari K, Marconi P, Martelli MF, Falzetti F, Di Ianni M
Number of Citations: 4
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific