Part of Thermo Fisher Scientific

Nicotinic Acetylcholine Receptor Monoclonal Antibody (88B)

Nicotinic Acetylcholine Receptor Antibody for IHC (F), IP, Western blot

Synonyms:
nAChR
Entrez Gene ID:
(Murine) 424940
UniProt ID:
P02717
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: 88B
Species Reactivity: Murine (Ms) Rat (Rt) Chicken (Ck) Amphibian (Am) Fish (Fs)
Immunogen: Purified Torpedo californica acetylcholine receptor.
Ordering Information
Pierce Nicotinic Acetylcholine Receptor Monoclonal Antibody (88B)
Product Number Pkg. Size Price Purchase
MA3-043 100 µl $350.00
Add Nicotinic Acetylcholine Receptor Monoclonal Antibody (88B) to your cart.


Storage: -20º C, Avoid Freeze/Thaw Cycles
Form: 100 µl of PBS-diluted ascites containing 0.05% sodium azide.


Applications Dilution *
Western Blot (WB) 1:5,000
Immunohistochemistry (Frozen) (IHC (F)) 1:1,000
Immunoprecipitation (IP) Assay dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
MA3-043 detects nicotinic acetylcholine receptor (nAChR) gamma and delta subunits in torpedo and the delta subunit in mouse, rat, chicken and frog. This antibody does not detect the alpha 1 and beta 1 subunits.

MA3-043 has been successfully used in Western blot, immunohistochemistry and immunoprecipitation procedures. By Western blot, this antibody detects a 60 kDa protein representing the gamma subunit and a 65 kDa protein representing the delta subunit from torpedo skeletal muscle homogenates. Under non-reducing conditions the delta subunit migrates mostly as a dimer of ~130 kDa. Immunohistochemical staining of nAChR in rat skeletal muscle with MA3-043 results in strong staining of the motor endplate.

In Immunohistochemical staining, cryostat or permeabilization of the sections is recommended due to the reactivity of this antibody with the cytoplasmic side of the receptor.

The MA3-043 antigen is purified Torpedo californica acetylcholine receptor.
General Information
Acetylcholine receptors (AChRs) mediate synaptic transmission at the neuromuscular junction. The channel-linked AChR that mediates rapid, excitatory actions of acetylcholine is called nicotinic AChR (nAChR) because it can be activated by nicotine. The non-channel linked AChR that medicates the slow actions of acetylcholine, which can be either inhibitory or excitatory, is called muscarinic AChR (mAChR) because it can be activated by muscarine.

The nAChRs fall into two subclasses, the muscle-type and the neuronal-type. The muscle-type receptor is a pentameric glycoprotein of five membrane spanning subunits, (2 alpha 1’s, beta 1, gamma or epsilon and delta) which form a ligand gated ion channel. The neuronal nAChR is also a pentamer, but unlike the muscle-type, is made up of only two general types of subunits, alpha and beta of which multiple subtypes have been described (alpha 2 to alpha 8 and beta 2 to beta 4).
PubMed References:
ELISA
  Species / Dilution Summary
  Rt / Not Cited
 MA3-043 was used in ELISA to investigate dystrophin's localization, relative abundance and stability in AChR clusters isolated from primary cultures of neonatal rat myotubes
J Neurosci. 1993 Feb;13(2):547-58.
"Dystrophin in a membrane skeletal network: localization and comparison to other proteins."
Author(s): Dmytrenko GM, Pumplin DW, Bloch RJ
Number of Citations: 3
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Rt / 20 nM
 MA3-043 was used in immunocytochemistry to investigate the interaction of acetylcholine receptors with peripheral membrane proteins
J Membr Biol. 1994 Feb;138(1):13-28.
"Association of acetylcholine receptors with peripheral membrane proteins: evidence from antibody-induced coaggregation."
Author(s): Bloch RJ, Sealock R, Pumplin DW, Luther PW, Froehner SC
Number of Citations: 0
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Am / 1:50
Rt / 1:25
 MA3-043 was used in immunoprecipitation, western blot, immunohistochemistry to characterize the monoclonal antibodies specific for different subunits of AChR and study the antigenic determinants of AChR
J Biol Chem. 1983 Jun 10;258(11):7112-20.
"Monoclonal antibodies to cytoplasmic domains of the acetylcholine receptor."
Author(s): Froehner SC, Douville K, Klink S, Culp WJ
Number of Citations: 26
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Ch / Not Cited
 MA3-043 was used in immunoprecipitation, western blot, immunohistochemistry to characterize the monoclonal antibodies specific for different subunits of AChR and study the antigenic determinants of AChR
J Biol Chem. 1983 Jun 10;258(11):7112-20.
"Monoclonal antibodies to cytoplasmic domains of the acetylcholine receptor."
Author(s): Froehner SC, Douville K, Klink S, Culp WJ
Number of Citations: 26
(See PubMed article )
  Rb / Not Cited
 MA3-043 was used in immunoprecipitation to characterize the dihydropyridine-sensitive calcium channel
J Biol Chem. 1987 Sep 5;262(25):11904-7.
"Monoclonal antibody identifies a 200-kDa subunit of the dihydropyridine-sensitive calcium channel."
Author(s): Morton ME, Froehner SC
Number of Citations: 17
(See PubMed article )
Western Blot
  Species / Dilution Summary
  Ch / 1:100
 MA3-043 was used in immunoprecipitation, western blot, immunohistochemistry to characterize the monoclonal antibodies specific for different subunits of AChR and study the antigenic determinants of AChR
J Biol Chem. 1983 Jun 10;258(11):7112-20.
"Monoclonal antibodies to cytoplasmic domains of the acetylcholine receptor."
Author(s): Froehner SC, Douville K, Klink S, Culp WJ
Number of Citations: 26
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)