* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Antigen affinity chromatography
0.1M tris, pH 7,
20% glycerol, 0.1M glycine
-20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA5-29748 targets Neurofibromin 2 in IHC (P) and WB applications and shows reactivity with Human, mouse, and Rat samples.
The PA5-29748 immunogen is recombinant protein fragment corresponding to a region within amino acids 1 and 172 of Human Neurofibromin 2.
This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that are thought to link cytoskeletal components with proteins in the cell membrane. This gene product has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. This gene is expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities. Two predominant isoforms and a number of minor isoforms are produced by alternatively spliced transcripts.
Neurofibromin 2 Antibody (PA5-29748) in WB Neurofibromin-2_PA5-29748_Antibody_WesternBlot.jpg
Western Blot with anti-Neurofibromin 2 Polyclonal Antibody (PA5-29748)
Western Blot analysis using anti-Neurofibromin 2 Polyclonal Antibody on the following cells: (30 ug of whole cell lysate) A: A431 cell lysate B: H1299 C: Hela. Primary antibody (PA5-29748) diluted at 1:1000
Neurofibromin 2 Antibody (PA5-29748) in IHC Neurofibromin-2_PA5-29748_Antibody_Immunohistochemistry.jpg
Immunohistochemistry with anti-Neurofibromin 2 Polyclonal Antibody (PA5-29748)