Nestin Antibody (10C2)

Nestin Monoclonal Antibody for Western blot, IF, ICC, IHC (P), FACS

>> See 15 other antibodies for NES (NES, FLJ21841, Nbla00170)
Synonyms:
NES, FLJ21841, Nbla00170
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG
Class: Monoclonal
Type: Antibody
Clone: 10C2
Tested Species Reactivity: Human (Hu), Mouse (Ms)
Immunogen: Human nestin protein corresponding to residues 1464-1614
Ordering Information
Pierce Nestin Antibody (10C2)
Product #
MA1-110
Size
100 µg
Price
$316.00
Purchase
Add Nestin Antibody (10C2) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:500 - 1:1500
Immunofluorescence (IF) 1:20 - 1:250
Immunocytochemistry (ICC) 1:100 - 1:250
Immunohistochemistry (Paraffin) (IHC (P)) 1:20-1:100
Flow Cytometry (FACS) 1 ug/test
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Protein A
Storage Buffer: PBS with 1mg/ml BSA, 30% glycerol
Preservative: 0.05% sodium azide
Storage Conditions: -20°C
Product Specific Information
MA1-110 has been successfully utilized in Western Blot, immunocytochemistry, flow cytometry, and immunofluorescence applications in human samples. MA1-110 also acts a neuronal stem cell marker and detects a band in Western blot at ~250 kDa.
General Information
This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells.
Product Images
  • Nestin Antibody (MA1-110) in WB
    MA1-110_Nestin_10C2-_WB_[1].jpg

    Western Blot with anti-Nestin Monoclonal Antibody [10C2] (MA1-110) (MA1-110_Nestin_10C2-_WB_[1].jpg)

    Western Blot with anti-Nestin Monoclonal Antibody [10C2] (MA1-110)

    Western blot analysis of Nestin was performed by loading 25 ug of the indicated whole cell lysates and 5ul of the Lane Marker Reducing Sample Buffer (Product # 39000) per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a Nestin monoclonal antibody (Product # MA1-110) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgG-HRP secondary antibody (Product # 32430) at a dilution of 1:15,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).
  • Nestin Antibody (MA1-110) in WB
    MA1-110_WB_20140114134418.jpg

    Western Blot with anti-Nestin Monoclonal Antibody [10C2] (MA1-110) (MA1-110_WB_20140114134418.jpg)

    Western Blot with anti-Nestin Monoclonal Antibody [10C2] (MA1-110)

    Western blot analysis of Nestin was performed by loading 25 ug of SH-SY5Y cell lysate onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a Nestin monoclonal antibody (Product # MA1-100) at a dilution of 1:100 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~250 kDa.
  • Nestin Antibody (MA1-110) in IF
    MA1110_IF.jpg

    Immunofluorescence with anti-Nestin Monoclonal Antibody [10C2] (MA1-110) (MA1110_IF.jpg)

    Immunofluorescence with anti-Nestin Monoclonal Antibody [10C2] (MA1-110)

    Immunofluorescent analysis of Nestin (green) U2-0S cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 0.3% BSA/TBST (Product # 37525) for 15 minutes at room temperature. Cells were probed with a Nestin monoclonal antibody (Product # MA1-110) at a dilution of 1:200 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat-anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
  • Nestin Antibody (MA1-110) in IF
    MA1-110 _IF_1.jpg

    Immunofluorescence with anti-Nestin Monoclonal Antibody [10C2] (MA1-110) (MA1-110 _IF_1.jpg)

    Immunofluorescence with anti-Nestin Monoclonal Antibody [10C2] (MA1-110)

    Immunofluorescent analysis of nestin (green) in U251 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a nestin Monclonal Antibody (Product # MA1-110 ) at a dilution of 1:20 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.
  • Nestin Antibody (MA1-110) in IF
    MA1-110 _IF_2.jpg

    Immunofluorescence with anti-Nestin Monoclonal Antibody [10C2] (MA1-110) (MA1-110 _IF_2.jpg)

    Immunofluorescence with anti-Nestin Monoclonal Antibody [10C2] (MA1-110)

    Immunofluorescent analysis of nestin (green) in human cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a nestin Monclonal Antibody (Product # MA1-110 ) at a dilution of 1:20 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.
  • Nestin Antibody (MA1-110) in IF
    MA1-110 _IF_3.jpg

    Immunofluorescence with anti-Nestin Monoclonal Antibody [10C2] (MA1-110) (MA1-110 _IF_3.jpg)

    Immunofluorescence with anti-Nestin Monoclonal Antibody [10C2] (MA1-110)

    Immunofluorescent analysis of nestin (green) in murine cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a nestin Monclonal Antibody (Product # MA1-110 ) at a dilution of 1:20 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.
  • Nestin Antibody (MA1-110) in IHC
    MA1-110_IHC_Human_Tonsil tissue.jpg

    Immunohistochemistry with anti-Nestin Monoclonal Antibody [10C2] (MA1-110) (MA1-110_IHC_Human_Tonsil tissue.jpg)

    Immunohistochemistry with anti-Nestin Monoclonal Antibody [10C2] (MA1-110)

    Immunohistochemistry was performed on human tonsil tissue. To expose target protein, antigen was retreived using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a nestin Rabbit polyclonal antibody (Product # MA1-110) at a dilution of 1:100 overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.
  • Nestin Antibody (MA1-110) in IHC
    MA1-110_IHC_Human_Skin tissue.jpg

    Immunohistochemistry with anti-Nestin Monoclonal Antibody [10C2] (MA1-110) (MA1-110_IHC_Human_Skin tissue.jpg)

    Immunohistochemistry with anti-Nestin Monoclonal Antibody [10C2] (MA1-110)

    Immunohistochemistry was performed on human skin tissue. To expose target protein, antigen was retreived using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a nestin Rabbit polyclonal antibody (Product # MA1-110) at a dilution of 1:20 overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.
  • Nestin Antibody (MA1-110) in IHC
    MA1-110_IHC_Human_Kidney tissue.jpg

    Immunohistochemistry with anti-Nestin Monoclonal Antibody [10C2] (MA1-110) (MA1-110_IHC_Human_Kidney tissue.jpg)

    Immunohistochemistry with anti-Nestin Monoclonal Antibody [10C2] (MA1-110)

    Immunohistochemistry was performed on human kidney tissue. To expose target protein, antigen was retreived using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a nestin Rabbit polyclonal antibody (Product # MA1-110) at a dilution of 1:100 overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.
  • Nestin Antibody (MA1-110) in FACS
    MA1-110_FACS_Neuro-2a_20131209123705.jpg

    Flow Cytometry with anti-Nestin Monoclonal Antibody [10C2] (MA1-110) (MA1-110_FACS_Neuro-2a_20131209123705.jpg)

    Flow Cytometry with anti-Nestin Monoclonal Antibody [10C2] (MA1-110)

    Flow cytometry analysis of Nestin in Neuro-2a cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a Nestin monoclonal antibody (Product # MA1-110) at a dilution of 1 ug/test for 40 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.
  • Nestin Antibody (MA1-110) in FACS
    MA1-110_FACS_SH-SY5Y_20131209123721.jpg

    Flow Cytometry with anti-Nestin Monoclonal Antibody [10C2] (MA1-110) (MA1-110_FACS_SH-SY5Y_20131209123721.jpg)

    Flow Cytometry with anti-Nestin Monoclonal Antibody [10C2] (MA1-110)

    Flow cytometry analysis of Nestin in SH-SY5Y cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a Nestin monoclonal antibody (Product # MA1-110) at a dilution of 1 ug/test for 40 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.
  • Nestin Antibody (MA1-110) in FACS
    MA1-110_FACS_U251_20131209123739.jpg

    Flow Cytometry with anti-Nestin Monoclonal Antibody [10C2] (MA1-110) (MA1-110_FACS_U251_20131209123739.jpg)

    Flow Cytometry with anti-Nestin Monoclonal Antibody [10C2] (MA1-110)

    Flow cytometry analysis of Nestin in U251 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a Nestin monoclonal antibody (Product # MA1-110) at a dilution of 1 ug/test for 40 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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