100 ul of antibody in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 ug/ml BSA and 50% glycerol.
Western Blot (WB)
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
PA5-17251 targets Na,K-ATPase in WB applications and shows reactivity with Hamster, Human, Murine, Non-human primate, zebrafish, and Rat samples.
The PA5-17251 immunogen is synthetic peptide corresponding to residues near the amino terminus of human Na,K-ATPase alpha1 subunit.
PA5-17251 detects Na,K-ATPase which has a predicted molecular weight of approximately 100 kDa.
The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.
Na,K-ATPase Antibody (PA5-17251) in WB
Western Blot with anti-Na,K-ATPase Polyclonal Antibody (PA5-17251)