NMDA Receptor 2B Antibody (NR2B)

NMDA Receptor 2B Monoclonal Antibody for Western blot, ICC, IHC (P)

>> See 16 other antibodies for GRIN2B (NR2B, N-Methyl-D-Aspartate Receptor Type 2B)
Synonyms:
NR2B, N-Methyl-D-Aspartate Receptor Type 2B
Entrez Gene ID:
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: NR2B
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt)
Published Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt)
Immunogen: Recombinant protein: NR2B subunit of amino acid residues 934-1457.
Ordering Information
Pierce NMDA Receptor 2B Antibody (NR2B)
Product #
MA1-2014
Size
100 µg
Price
$360.00
Purchase
Add NMDA Receptor 2B Antibody (NR2B) to your cart.
Tested Applications Dilution *
Western Blot (WB) 2 ug/ml
Immunocytochemistry (ICC) 1 ug/ml
Immunohistochemistry (Paraffin) (IHC (P)) 1:10-1:100
Published Applications Dilution
Western Blot (WB) See publications below
Immunocytochemistry (ICC) See publications below
Immunoprecipitation (IP) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Protein A
Storage Buffer: PBS with 1mg/ml BSA
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
MA1-2014 detects the N-methyl-D-aspartate (NMDA) receptor type 2B in human and mouse samples.

MA1-2014 has been used successfully in Western blot, IHC (P) and immunocytochemistry procedures. In Western blot analysis of mouse brain tissue this antibody detects a ~166 kDa protein representing NMDA receptor type 2B.

The MA1-2014 immunogen is a recombinant protein composed of amino acid residues 934-1457 of the rat NMDA receptor type 2B.
General Information
N-methyl-D-aspartate (NMDA) receptors are ligand-gated ion channels that have a high permeability to calcium found in the central nervous system. The receptor consists of a number of distinct ligand binding domains, and the presence of both glutamate and glycine are required for full activation of the channel. Within the channel there is also a binding site for magnesium, which, when occupied, propagates a voltage-dependent channel block. Other binding sites are also found in the receptor, including a zinc-binding site and an inter-channel site that binds specific channel blockers such as phencyclidine (PCP) and related compounds. The NMDA receptor has been demonstrated to play an essential role in long-term potentiation (LTP), a phenomenon that has been implicated to be the basis for learning and memory. The influx of calcium as a result of channel activation is thought to be responsible for neuronal plasticity and glutamate neurotoxicity.

A number of different NMDA receptor subunits have been cloned that may possess different functional and localization properties. The NMDA-R1 subunit (NR1) is expressed throughout the brain, while the NMDA-R2 subunits (NR2A, NR2B, NR2C, and NR2D) have a more specific localization pattern. The NMDA receptor subunits differ also in glycine sensitivity, the relative strength of the magnesium channel block, and their respective agonist-dependent deactivation time. Differential splicing of three distinct exons of NR1 generates eight NR1 splice variants. These exons encode a 21 amino acid N-terminal domain (N1) and two 21 amino acid C-terminal domains (C1 and C2, respectively). Splicing out the C2 cassette eliminates a stop codon, generating a new 22 amino acid C-terminal domain (C2'). These splice variants have differing patterns of expression.
Product Images
  • NMDA Receptor 2B Antibody (MA1-2014) in WB
    GRIN2B-Antibody-MA1-2014-WesternBlot-WB.jpg

    Western Blot with anti-NMDA Receptor 2B Monoclonal Antibody [NR2B] (MA1-2014) (GRIN2B-Antibody-MA1-2014-WesternBlot-WB.jpg)

    Western Blot with anti-NMDA Receptor 2B Monoclonal Antibody [NR2B] (MA1-2014)

    Western blot detection of NMDA receptor type 2B in mouse brain tissue using MA1-2014.
  • NMDA Receptor 2B Antibody (MA1-2014) in IHC (P)
    MA1-2014_IHC_human_breast.jpg

    Immunohistochemistry (Paraffin) with anti-NMDA Receptor 2B Monoclonal Antibody [NR2B] (MA1-2014) (MA1-2014_IHC_human_breast.jpg)

    Immunohistochemistry (Paraffin) with anti-NMDA Receptor 2B Monoclonal Antibody [NR2B] (MA1-2014)

    Immunohistochemistry analysis of NMDA Receptor 2B showing staining in the cytoplasm and membrane of paraffin-embedded human breast tissue (right) compared with a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a NMDA Receptor 2B monoclonal antibody (Product # MA1-2014) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Publications:
Western Blot
  Species / Dilution Summary
  Ms / 1 ug/ml
MA1-2014 was used in immunoprecipitation and western blot to study the roles of NMDA-type glutamate receptors in the degeneration of striatal medium-sized spiny neurons in Huntington's disease.

J Neurosci. 2009 Sep 2;29(35):10928-38.
"Interaction of postsynaptic density protein-95 with NMDA receptors influences excitotoxicity in the yeast artificial chromosome mouse model of Huntington's disease."
Author(s): Fan J, Cowan CM, Zhang LY, Hayden MR, Raymond LA
Number of Citations: 1
(See PubMed article )
  Ms / 0
MA1-2014 was used in immunoprecipitation and western blot to study the role of post-synaptic density 95 in N-methyl-D-aspartate receptor signaling pathway

J Neurochem. 2010 Nov;115(4):1045-56.
"N-methyl-D-aspartate receptor subunit- and neuronal-type dependence of excitotoxic signaling through post-synaptic density 95."
Author(s): Fan J, Vasuta OC, Zhang LY, Wang L, George A, Raymond LA
Number of Citations: 1
(See PubMed article )
  Ms / 0
MA1-2014 was used in immunoprecipitation and western blot to investigate the protective effect of Tat-NR2B9c in YAC Huntingtin disease mice

Neurobiol Dis. 2012 Mar;45(3):999-1009.
"P38 MAPK is involved in enhanced NMDA receptor-dependent excitotoxicity in YAC transgenic mouse model of Huntington disease."
Author(s): Fan J, Gladding CM, Wang L, Zhang LY, Kaufman AM, Milnerwood AJ, Raymond LA
Number of Citations: 1
(See PubMed article )
  Ms / 1:1000
MA1-2014 was used in western blot to study changes in NMDAR signaling and apoptosis in cortico-striatal co-cultures from Huntington's disease mice

Neurobiol Dis. 2012 Oct;48(1):40-51.
"Mitigation of augmented extrasynaptic NMDAR signaling and apoptosis in cortico-striatal co-cultures from Huntington's disease mice."
Author(s): Milnerwood AJ, Kaufman AM, Sepers MD, Gladding CM, Zhang L, Wang L, Fan J, Coquinco A, Qiao JY, Lee H, Wang YT, Cynader M, Raymond LA
Number of Citations: 0
(See PubMed article )
  Rt / 0.5-1 ug/ml
MA1-2014 was used in western blot to investigate the localization of NMDA receptor subunits in the rat brain and the corresponding expression changes during development using novel subunit-specific antibodies

J Neurochem. 1995 Jul;65(1):176-83.
"Characterization of NMDA receptor subunit-specific antibodies: distribution of NR2A and NR2B receptor subunits in rat brain and ontogenic profile in the cerebellum."
Author(s): Wang YH, Bosy TZ, Yasuda RP, Grayson DR, Vicini S, Pizzorusso T, Wolfe BB
Number of Citations: 10
(See PubMed article )
  Rt / 1:1000
MA1-2014 was used in western blot to study NMDA receptor gene responses to ischemia

Neurochem Int. 2010 Jul;56(8):878-85.
"Age and meloxicam attenuate the ischemia/reperfusion-induced down-regulation in the NMDA receptor genes."
Author(s): Montori S, Dos-Anjos S, Martínez-Villayandre B, Regueiro-Purriños MM, Gonzalo-Orden JM, Ruano D, Fernández-López A
Number of Citations: 0
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Hu / 0
MA1-2014 was used in immunocytochemistry to study the role TRPV1 as a synaptic protein regulating vesicle recycling

J Cell Sci. 2010 Jun 15;123(Pt 12):2045-57.
"TRPV1 acts as a synaptic protein and regulates vesicle recycling."
Author(s): Goswami C, Rademacher N, Smalla KH, Kalscheuer V, Ropers HH, Gundelfinger ED, Hucho T
Number of Citations: 2
(See PubMed article )
  Rt / 1:800
MA1-2014 was used in immunocytochemistry to study the Ca(2+) increase in neuronal cells induced by the non-specific interaction of pre-fibrillar amyloid aggregates with glutaminergic receptors

J Biol Chem. 2008 Oct 31;283(44):29950-60.
"Nonspecific interaction of prefibrillar amyloid aggregates with glutamatergic receptors results in Ca2+ increase in primary neuronal cells."
Author(s): Pellistri F, Bucciantini M, Relini A, Nosi D, Gliozzi A, Robello M, Stefani M
Number of Citations: 8
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Ms / 1 ug/ml
MA1-2014 was used in immunoprecipitation and western blot to study the roles of NMDA-type glutamate receptors in the degeneration of striatal medium-sized spiny neurons in Huntington's disease.

J Neurosci. 2009 Sep 2;29(35):10928-38.
"Interaction of postsynaptic density protein-95 with NMDA receptors influences excitotoxicity in the yeast artificial chromosome mouse model of Huntington's disease."
Author(s): Fan J, Cowan CM, Zhang LY, Hayden MR, Raymond LA
Number of Citations: 1
(See PubMed article )
  Ms / 0
MA1-2014 was used in immunoprecipitation and western blot to study the role of post-synaptic density 95 in N-methyl-D-aspartate receptor signaling pathway

J Neurochem. 2010 Nov;115(4):1045-56.
"N-methyl-D-aspartate receptor subunit- and neuronal-type dependence of excitotoxic signaling through post-synaptic density 95."
Author(s): Fan J, Vasuta OC, Zhang LY, Wang L, George A, Raymond LA
Number of Citations: 1
(See PubMed article )
  Ms / 0
MA1-2014 was used in immunoprecipitation and western blot to investigate the protective effect of Tat-NR2B9c in YAC Huntingtin disease mice

Neurobiol Dis. 2012 Mar;45(3):999-1009.
"P38 MAPK is involved in enhanced NMDA receptor-dependent excitotoxicity in YAC transgenic mouse model of Huntington disease."
Author(s): Fan J, Gladding CM, Wang L, Zhang LY, Kaufman AM, Milnerwood AJ, Raymond LA
Number of Citations: 1
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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