NMDA Receptor 2B Antibody

NMDA Receptor 2B Polyclonal Antibody for Western blot, IF, ICC, IHC, IP, ELISA

>> See 16 other antibodies for GRIN2B (NR2B, N-Methyl-D-Aspartate Receptor Type 2B)
Synonyms:
NR2B, N-Methyl-D-Aspartate Receptor Type 2B
Entrez Gene ID:
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt)
Immunogen: Synthetic phosphopeptide corresponding to residues C G(1328) R F M D G S P Y(p) H(1337) of rat NMDA receptor 2B.
Ordering Information
Pierce NMDA Receptor 2B Antibody
Product #
PA3-105
Size
200 µl
Price
$316.00
Purchase
Add NMDA Receptor 2B Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:1000
Immunofluorescence (IF) 1:500
Immunocytochemistry (ICC) 1:500
Immunohistochemistry (IHC) 1:500
Immunoprecipitation (IP) Assay dependent
ELISA (ELISA) 1:50,000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Storage Buffer: whole serum
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA3-105 detects N-methyl-D-aspartate (NMDA) receptor type 2B.

PA3-105 has been used successfully in Western blot, ELISA, immunoprecipitation, immunohistochemistry, and immunocytochemistry procedures. In Western blot analysis of rat brain synaptic membranes this antibody detects a ~180 kDa protein representing NMDA receptor type 2B.

The PA3-105 immunogen is a synthetic phosphopeptide corresponding to residues (C) G(1328) R F M D G S P Y(p) H(1337) of rat NMDA receptor 2B.

General Information
N-methyl-D-aspartate (NMDA) receptors are ligand-gated ion channels that have a high permeability to calcium found in the central nervous system. The receptor consists of a number of distinct ligand binding domains, and the presence of both glutamate and glycine are required for full activation of the channel. Within the channel there is also a binding site for magnesium, which, when occupied, propagates a voltage-dependent channel block. Other binding sites are also found in the receptor, including a zinc-binding site and an inter-channel site that binds specific channel blockers such as phencyclidine (PCP) and related compounds. The NMDA receptor has been demonstrated to play an essential role in long-term potentiation (LTP), a phenomenon that has been implicated to be the basis for learning and memory. The influx of calcium as a result of channel activation is thought to be responsible for neuronal plasticity and glutamate neurotoxicity.

A number of different NMDA receptor subunits have been cloned that may possess different functional and localization properties. The NMDA-R1 subunit (NR1) is expressed throughout the brain, while the NMDA-R2 subunits (NR2A, NR2B, NR2C, and NR2D) have a more specific localization pattern. The NMDA receptor subunits differ also in glycine sensitivity, the relative strength of the magnesium channel block, and their respective agonist-dependent deactivation time. Differential splicing of three distinct exons of NR1 generates eight NR1 splice variants. These exons encode a 21 amino acid N-terminal domain (N1) and two 21 amino acid C-terminal domains (C1 and C2, respectively). Splicing out the C2 cassette eliminates a stop codon, generating a new 22 amino acid C-terminal domain (C2'). These splice variants have differing patterns of expression.
Product Images
  • NMDA Receptor 2B Antibody (PA3-105) in IF
    Grin2b-Antibody-PA3-105-Immunofluorescence-IF.jpg

    Immunofluorescence with anti-NMDA Receptor 2B Polyclonal Antibody (PA3-105) (Grin2b-Antibody-PA3-105-Immunofluorescence-IF.jpg)

    Immunofluorescence with anti-NMDA Receptor 2B Polyclonal Antibody (PA3-105)

    Immunofluorescence image of NMDA receptor type 2B in rat brain tissue using PA3-105.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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