| Details | |
| Host / Isotype: | Mouse / IgG1, kappa |
| Class: | Monoclonal |
| Type: | Antibody |
| Label: | Biotin |
| Species Reactivity: | Human (Hu ) |
| Immunogen: | Human recombinant NGAL in monomer and dimer forms. |
| Ordering Information | ||||
| Pierce NGAL Antibody |
| Storage: | 4° C, store in dark, DO NOT FREEZE! |
| Form: | 50 ug of biotinylated antibody (1 mg/ml) in 0.01 M phosphate buffer with 0.14 M NaCl and 15 mM sodium azide; pH 7.4. |
| Applications | Dilution * |
| ELISA (ELISA) | 1:1,000 |
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* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
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| Product Specific Information |
| HYB 211-01B-005 is specific for human NGAL. Crossreactivity to other species has not been detected. HYB 211-01B-005 has been successfully used in ELISA procedures. The HYB 211-01B-005 immunogen is human recombinant NGAL in monomer and dimer forms. |
| General Information |
| Neutrophil gelatinase-associated lipocalin is a member of the lipocalin family of proteins which bind and transport small lipophilic molecules. NGAL is released by activated neutrophils and occurs as 25 kDa glycosylated single protein chain monomers, which form dimers and small amounts of higher oligomers, as well as complexes with matrix metalloproteinase 9. Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers. Expression of NGAL in the kidney is dramatically increased by acute kidney injury. |