Human (Hu) Mouse (Ms) Rat (Rt) Non-human primate (Nhp)
Pierce NFKB1 Antibody
Western Blot (WB)
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
82387 targets NFKB1 in WB applications and shows reactivity with Human, Murine, Non-human primate, and Rat samples.
The 82387 immunogen is nFkB 1.
82387 detects NFKB1 which has a predicted molecular weight of approximately 105, 50 kDa.
This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for this gene.
NFKB1 Antibody (82387) in WB
Western Blot with anti-NFKB1 Polyclonal Antibody (82387)
A549 cells were lysed 72 hours after transfection. Cells were transfected with Transfection Reagent alone (Lane 1), 100nM ON-TARGETplus siCONTROL Non-Targeting Pool (Lane 2), or 100nM ON-TARGETplus NFKB1 siRNA (Lane 3). Western blot data for GAPDH is included as a control for equal protein loading.